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http://dx.doi.org/10.5713/ajas.2007.761

Intramuscular Administration of Zinc Metallothionein to Preslaughter Stressed Pigs Improves Anti-oxidative Status and Pork Quality  

Li, L.L. (Laboratory of Animal Nutrition and Health and Key Laboratory of subtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences)
Hou, Z.P. (Laboratory of Animal Nutrition and Health and Key Laboratory of subtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences)
Yin, Y.L. (Laboratory of Animal Nutrition and Health and Key Laboratory of subtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences)
Liu, Y.H. (Laboratory of Animal Nutrition and Health and Key Laboratory of subtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences)
Hou, D.X. (Department of Biochemical Technology, Faculty of Agriculture, Kagoshima University)
Zhang, B. (College of Animal Science and Technology, Hunan Agricultural University)
Wu, G.Y. (Laboratory of Animal Nutrition and Health and Key Laboratory of ubtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences)
Kim, S.W. (Department of Animal and Food Sciences, Texas Tech University)
Fan, M.Z. (Department of Animal and Poultry Science, University of Guelph)
Yang, C.B. (Laboratory of Animal Nutrition and Health and Key Laboratory of ubtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences)
Kong, X.F. (Laboratory of Animal Nutrition and Health and Key Laboratory of ubtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences,)
Tang, Z.R. (Laboratory of Animal Nutrition and Health and Key Laboratory of ubtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences)
Peng, H.Z. (College of Animal Science and Technology, Hunan Agricultural University)
Deng, D. (Laboratory of Animal Nutrition and Health and Key Laboratory of ubtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences)
Deng, Z.Y. (The key Laboratory of Food Science of Ministry of Education, Nanchang University)
Xie, M.Y. (The key Laboratory of Food Science of Ministry of Education, Nanchang University)
Xiong, H. (The key Laboratory of Food Science of Ministry of Education, Nanchang University)
Kang, P. (Laboratory of Animal Nutrition and Health and Key Laboratory of ubtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences)
Wang, S.X. (Laboratory of Animal Nutrition and Health and Key Laboratory of ubtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.20, no.5, 2007 , pp. 761-767 More about this Journal
Abstract
This study was conducted to determine the effects of exogenous zinc-metallothionein (Zn-MT) on anti-oxidative function and pork quality. After feeding a corn-soybean meal-based diet for two weeks, 48 pigs ($Duroc{\times}Landrace{\times}Chinese\;Black Pig$) were assigned randomly to four groups. Pigs in Group 1 were maintained under non-stress conditions, whereas pigs in Groups 2, 3 and 4 were aggressively handled for 25 min to produce stress. Pigs in Groups 1, 2, 3, and 4 received intramuscular administration of saline (control group; CON), 0 (negative control group; NCON), 0.8 (low dose group; LOW), and 1.6 (high dose group; HIGH) mg rabbit liver Zn-MT per kg body weight, respectively. Pigs were slaughtered at 3 and 6 h post-injection. Zn-MT treatment increased (p<0.05) the activities of superoxide dismutase (SOD) and glutathione-peroxidase (GSH-PX) while decreasing the concentration of malondialdehyde (MDA) in liver. These responses were greater (p<0.05) at 6 h than at 3 h post Zn-MT injection. Zn-MT treatment increased (p<0.05) hepatic SOD mRNA levels in a time and dose-dependent manner and decreased (p<0.05) serum glutamate-pyruvate transaminase and lactate dehydrogenase activities (indicators of tissue integrity). Zn-MT administration decreased (p<0.05) lactate concentration and increased (p<0.05) pH and water-holding capacity in the longissimus thorasis meat. Collectively, our results indicate that intramuscular administration of Zn-MT to pre-slaughter stressed pigs improved tissue anti-oxidative ability and meat quality.
Keywords
Stress; Metallothionein; Anti-oxidative Enzyme; Superoxide Dismutase Gene Expression; Pork Meat Quality;
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Times Cited By Web Of Science : 25  (Related Records In Web of Science)
Times Cited By SCOPUS : 19
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