• Environmental Analysis Health and Toxicology
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• v.26
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• pp.5.1-5.11
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• 2011

Objectives: In order to identify the possibility of slender bitterling (SB) (Acheilognathus yamatsutae) being used as a test species for estrogenic endocrine disrupting chemicals (EEDCs), we carried out the cloning and sequence characterization of the estrogen receptor (ER). Methods: The ER from a slender bitterling was obtained by reverse transcriptase-polymerase chain reaction (RT-PCR), 5'- and 3'-rapid amplification of cDNA ends (5'-RACE and 3'-RACE) and T-vector cloning. The expression of ER mRNA was also analyzed in six tissues (brain, liver, kidney, gill, gonad, and intestines) by real-time PCR. Results: We obtained an ER from the slender bitterling. The SB ER cDNA was 2189 base pairs (bp) in length and contained a 1707 bp open reading frame that encoded 568 amino acid residues. The SB ER amino acid sequence clustered in a monophyletic group with the $ER{\alpha}$ of other fish, and was more closely related to zebrafish $ER{\alpha}$(88% identity) than to the $ER{\alpha}$ of other fish. The SB ER cDNA was divided into A/B, C, D, E and F domains. The SB ER has conserved important sequences for ER functions, such as the DNA binding domain (D domain), which are consistent with those of other teleosts. Conclusions: The ER of the slender bitterling could provide basic information in toxicological studies of EEDCs in the slender bitterling.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.8C
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• pp.788-799
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• 2007

This paper presents a watermarking method for copyright protection and illegal copy prevention of mobile 3D contents. The proposed method embeds copyright information and user's phone number into spatial domain and encryption domain of mobile animation data based on Buyer-Seller watermarking protocol. Furthermore, we insert user's operation key so that only authorized user can play 3D animation game in mobile device. We implemented the proposed method by using mobile animation tool, G3-SDK. From experimental results, we verified that the proposed method is capable of copyright protection and illegal copy prevention since the watermark can be well extracted against geometrical attacks, such as noise addition, data accuracy variableness and data up/down scaling.

• Microbiology and Biotechnology Letters
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• v.48 no.1
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• pp.24-31
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• 2020

Five genes (mxbDM, mxcQE and mxaB) are responsible for the transcription of methanol oxidation genes in Methylobacterium strains. Among these, MxbDM and MxcQE constitute the two-component system (TCS) regulating methanol metabolism. In this study, we integrated the methanol-sensing domain of MxbD and MxcQ with the EnvZ/OmpR from Escherichia coli. The domain-swapping strategy resulted in chimeric histidine kinases (HK's) MxbDZ and MxcQZ AM1 containing recombinant E. coli. Real-time quantitative PCR was used to monitor OmpC expression mediated by the chimeric HK and response regulator (RR) OmpR. Further, an ompC promoter based fluorescent biosensor for sensing methanol was developed. GFP fluorescence was studied both qualitatively and quantitatively in response to environmental methanol. GFP measurement also confirmed ompC expression. Maximum fluorescence was observed at 0.05% methanol and 0.01% methanol using MxbDZ and MxcQZ AM1, respectively. Thus the chimeric HK containing E. coli were found to be highly sensitive to methanol, resulting in a rapid response making them an ideal sensor.

• The Journal of the Acoustical Society of Korea
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• v.15 no.5
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• pp.21-29
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• 1996

Industrial equipment, such as power plant, is required to operate reliably, continuously and economically under rather severe conditions of temperature, stress, and enbironment. To test structural integrity and fitness, ultrasonic nondestructive testing is used because of effectiveness and simplicity. In this paper, wavelet transform based least mean square(LMS) algorithm is applied to reduce the influence of the interference occurring between randomly positioned small scatters. The RUN test is performed to check the nonstationarity of the speckle noise signal. The performance of this new approach is compared with that of the time domain LMS algorithm by means of condition numbers, signal-to-noise ratio and 3-D image. As a result, the wavelet transform based LMS algorithm shows better performance than the time domain LMS algorithm in this experiment.

• BMB Reports
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• v.37 no.5
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• pp.538-545
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• 2004

A new CRT binding factor (CBF) gene designated Cbcbf25 was cloned from Capsella bursa-pastoris, a wild grass, by the rapid amplification of cDNA ends (RACE). The full-length cDNA of Cbcbf25 was 898 bp with a 669 bp open reading frame (ORF) encoding a putative DRE/CRT (LTRE)-binding protein of 223 amino acids. The predicted CbCBF25 protein contained a potential nuclear localization signal (NLS) in its N-terminal region followed by an AP2 DNA-binding motif and a possible acidic activation domain in the C-terminal region. Bioinformatic analysis revealed that Cbcbf25 has a high level of similarity with other CBF genes like cbf1, cbf2, and cbf3 from Arabidopsis thaliana, and Bncbf5, Bncbf7, Bncbf16, and Bncbf17 from Brassica napus. A cold acclimation assay showed that Cbcbf25 was expressed immediately after cold triggering, but this expression was transient, suggesting that it concerns cold acclimation. Our study implies that Cbcbf25 is an analogue of other CBF genes and may participate in cold-response, by for example, controlling the expression of cold-regulated genes or increasing the freezing tolerance of plants.

• Kyungpook Mathematical Journal
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• v.55 no.3
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• pp.641-652
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• 2015

In this paper we study the problem of normal families of meromorphic functions concerning shared values. Let F be a family of meromorphic functions in the plane domain $D{\subseteq}{\mathbb{C}}$ and n, k be two positive integers such that $n{\geq}k+1$, and let a, b be two finite complex constants such that $a{\neq}0$. Suppose that (1) $f+a(f^{(k)})^n$ and $g+a(g^{(k)})^n$ share b in D for every pair of functions f, $g{\in}F$; (2) All zeros of f have multiplicity at least k + 2 and all poles of f have multiplicity at least 2 for each $f{\in}F$ in D; (3) Zeros of $f^{(k)}(z)$ are not the b points of f(z) for each $f{\in}F$ in D. Then F is normal in D. And some examples are provided to show the result is sharp.

• Molecules and Cells
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• v.42 no.7
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• pp.512-522
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• 2019

Chromosomes located in the nucleus form discrete units of genetic material composed of DNA and protein complexes. The genetic information is encoded in linear DNA sequences, but its interpretation requires an understanding of three-dimensional (3D) structure of the chromosome, in which distant DNA sequences can be juxtaposed by highly condensed chromatin packing in the space of nucleus to precisely control gene expression. Recent technological innovations in exploring higher-order chromatin structure have uncovered organizational principles of the 3D genome and its various biological implications. Very recently, it has been reported that large-scale genomic variations may disrupt higher-order chromatin organization and as a consequence, greatly contribute to disease-specific gene regulation for a range of human diseases. Here, we review recent developments in studying the effect of structural variation in gene regulation, and the detection and the interpretation of structural variations in the context of 3D chromatin structure.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.16 no.2
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• pp.49-52
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• 2006

Periodic electric field assisted poling low loss (${\sim}0.1dB/cm$) single-mode Ti-diffused waveguides in $LiNbO_3$ has been achieved using a periodically patterned electrode on the +Z surface of Ti : $LiNbO_3$ crystal and homogeneous LiCl solution. Using selective chemical etching, we confirmed the periodic (${\sim}16{\mu}m$) domain inverted structure and measured SH (second harmonic) properties of fabricated periodically poled Ti : $LiNbO_3$ waveguides.

• Molecules and Cells
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• v.37 no.4
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• pp.322-329
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• 2014

N-acetylglucosamine kinase (GlcNAc kinase or NAGK; EC 2.7.1.59) is highly expressed and plays a critical role in the development of dendrites in brain neurons. In this study, the authors conducted structure-function analysis to verify the previously proposed 3D model structure of GlcNAc/ATP-bound NAGK. Three point NAGK mutants with different substrate binding capacities and reaction velocities were produced. Wild-type (WT) NAGK showed strong substrate preference for GlcNAc. Conversion of Cys143, which does not make direct hydrogen bonds with GlcNAc, to Ser (i.e., C143S) had the least affect on the enzymatic activity of NAGK. Conversion of Asn36, which plays a role in domain closure by making a hydrogen bond with GlcNAc, to Ala (i.e., N36A) mildly reduced NAGK enzyme activity. Conversion of Asp107, which makes hydrogen bonds with GlcNAc and would act as a proton acceptor during nucleophilic attack on the ${\gamma}$-phosphate of ATP, to Ala (i.e., D107A), caused a total loss in enzyme activity. The overexpression of EGFP-tagged WT or any of the mutant NAGKs in rat hippocampal neurons (DIV 5-9) increased dendritic architectural complexity. Finally, the overexpression of the small, but not of the large, domain of NAGK resulted in dendrite degeneration. Our data show the effect of structure on the functional aspects of NAGK, and in particular, that the small domain of NAGK, and not its NAGK kinase activity, plays a critical role in the upregulation of dendritogenesis.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.25 no.3
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• pp.427-432
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• 2021

In this paper, we conduct performance analysis in automatic modulation classification of unknown communication signal to identify its modulation types based on deep neural network. The modulation classification performance was verified using time domain digital sample data of the modulated signal, frequency domain data to which FFT was applied, and time and frequency domain mixed data as neural network input data. For 11 types of analog and digitally modulated signals, the modulation classification performance was verified in various SNR environments ranging from -20 to 18 dB and reason for false classification was analyzed. In addition, by checking the learning speed according to the type of input data for neural network, proposed method is effective for constructing an practical automatic modulation recognition system that require a lot of time to learn.