• Mycobiology
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• 제37권1호
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• pp.62-66
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• 2009

The internal stipe necrosis of cultivated mushrooms (Agaricus bisporus) is caused by the bacterium Ewingella americana, a species of the Enterobacteriaceae. Recently, Ewingella americana was isolated from cultivated white button mushrooms in Korea evidencing symptoms of internal stipe browning. Its symptoms are visible only at harvest, and appear as a variable browning reaction in the center of the stipes. From these lesions, we isolated one bacterial strain (designated CH4). Inoculation of the bacterial isolate into mushroom sporocarps yielded the characteristic browning symptoms that were distinguishable from those of the bacterial soft rot that is well known to mushroom growers. The results of Gram stain, flagellal staining, and biochemical tests identified these isolates as E. americana. This was verified by pathogenicity, physiological and biochemical characteristics, and the results of an analysis of the 16S rRNA gene sequences and the fatty acids profile. This is the first report of the isolation of E. americana from cultivated white button mushrooms in Korea.

• Mycobiology
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• 제49권1호
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• pp.61-68
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• 2021

Agaricus bisporus, commonly known as the button mushroom, is widely cultivated throughout the world. To breed new strains with more desirable traits and improved adaptability, diverse germplasm, including wild accessions, is a valuable genetic resource. To better understand the genetic diversity available in A. bisporus and identify previously unknown diversity within accessions, a phylogenetic analysis of 360 Agaricus spp. accessions using single-nucleotide polymorphism genotyping was performed. Genetic relationships were compared using principal coordinate analysis (PCoA) among accessions with known origins and accessions with limited collection data. The accessions clustered into four groups based on the PCoA with regard to genetic relationships. A subset of 67 strains, which comprised a core collection where repetitive and uninformative accessions were not included, clustered into 7 groups following analysis. Two of the 170 accessions with limited collection data were identified as wild germplasm. The core collection allowed for the accurate analysis of A. bisporus genetic relationships, and accessions with an unknown pedigree were effectively grouped, allowing for origin identification, by PCoA analysis in this study.

• Mycobiology
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• 제47권4호
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• pp.527-532
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• 2019

We designed 170 new simple sequence repeat (SSR) markers based on the whole-genome sequence data of button mushroom (Agaricus bisporus), and selected 121 polymorphic markers. A total of 121 polymorphic markers, the average major allele frequency (M_AF) and the average number of alleles (N_A) were 0.50 and 5.47, respectively. The average number of genotypes (N_G), observed heterozygosity (H_O), expected heterozygosity (H_E), and polymorphic information content (PIC) were 6.177, 0.227, 0.619, and 0.569, respectively. Pearson's correlation coefficient showed that M_AF was negatively correlated with N_G (-0.683), N_A (-0.600), H_O (-0.584), and PIC (-0.941). N_G, N_A, H_O, and PIC were positively correlated with other polymorphic parameters except for M_AF. UPGMA clustering showed that 26 A. bisporus accessions were classified into 3 groups, and each accession was differentiated. The 121 SSR markers should facilitate the use of molecular markers in button mushroom breeding and genetic studies.

• 한국버섯학회지
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• 제12권3호
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• pp.163-170
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• 2014

The potential ability of Button mushroom compost (BMC) to solubilize heavy metals was estimated with metal contaminated soils collected from abandoned mines of Boryeong area in South Korea. The bacterial strains in BMC were isolated for investigating the mobilization of metals in soil or plant by the strains and identified according to 16S rRNA gene sequence analysis. When metal solubilization potential of BMC was assessed in a batch experiment, the BMC was found to be capable of solubilizing metals in the presence of metals (Co, Pb and Zn) and the results showed that inoculation of BMC could increase the concentrations of water soluble Co, Pb and Cd by 35, 25 and 45% respectively, than those of non-inoculated soils. BMC-assisted growth promotion and metal uptake in sunflower (Helianthus annuus) was also evaluated in a pot experiment. In comparison with non-inoculated seedlings, the inoculation led to increase the growth of H. annuus by 27, 25 and 28% respectively in Co, Pb and Zn contaminated soils. Moreover, enhanced accumulation of Co, Pb and Zn in the shoot and root systems was observed in inoculated plants, where metal translocation from root to the above-ground tissues was also found to be enhanced by the BMC. The apparent results suggested that the BMC could effectively be employed in enhancing phytoextraction of Co, Pb and Zn from contaminated soils.

• 한국버섯학회지
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• 제15권4호
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• pp.254-258
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• 2017

과거 돌발적으로 피해를 주었던 버섯응애류가 최근 양송이 주산지를 중심으로 빈번하게 발생하여 피해 농가가 확산되고 있다. 버섯응애류 방제용으로 등록된 약제는 아직까지 없으며 적용 가능한 살충제가 있다고 하더라도 잔류 문제로 인하여 사용 시기에 제약이 많아 방제에 어려움이 있다. 따라서 양송이 생장에는 영향이 없이 안전하면서 버섯파리, 버섯응애류만 선택적으로 방제할 수 있는 친환경 방제기술 개발이 절실하다. 본 연구에서는 버섯파리를 포식하는 포식성 천적 응애 스키미투스응애를 이용한 버섯응애류의 생물적 방제효과를 검정하였다. 그 결과 스키미투스응애 30,000마리(3병)를 재배사 $165{\sim}230m^2$ 기준으로 균상 준비 전 물청소 후 1회, 균 접종 후 1회, 복토 전후 1회 등 3회를 1~2 m 간격으로 골고루 균상 위에 처리하면 버섯응애류 방제를 효과적으로 할 수 있을 것으로 판단되며 스키미투스응애의 처리만으로 양송이의 주요 해충인 버섯파리의 알, 유충, 번데기, 버섯응애류를 동시에 방제할 수 있으며 작기 중 사용 시기에 구애받지 않고 사용할 수 있는 것이 장점이다.

• 농업과학연구
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• 제4권1호
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• pp.43-50
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• 1977

1. 본(本) 연구(硏究)는 양송이의 재배(栽培) 및 수확방법(收穫方法)이 다를 때 자실체발생(子實體發生)의 변동상(變動相)을 밝히고 수량(收量)의 조기예측방법(早期豫測方法)을 확립(確立)하고자 실시(實施)하였다. 2. 양송이 수량(收量)은 최초(最初) 30일간(日間)의 채취량(採取量)과 상관(相關)이 높고 다수확재배사(多收穫栽培舍)의 수량구성(收量構成)에 있어서는 중후기(中後期)의 채취량(採取量)과 다소(多小)가 큰 역할(役割)을 하였다. 3 채취시기(採取時期)에 따른 수량(收量)의 변화(變化)는 재배시기(栽培時期)에 따라 다르나 작업관리(作業管理)가 정상상태인 추기재배(秋期栽培) 때는 어린 버섯을 채취(採取)하므로서 초기수량(初期收量)은 감소(減少)하고 후기채취량(後期採取量)은 증가(增加)하였다. 4. Button 채취비율(採取比率) 20~45% 범위(範圍)에서 전수량(全收量)이 증가(增加)하면 Button 채취비율(採取比率)도 증가(增加)하는 경향(碩向)을 보였다. 5 양송이 재배합(栽培舍)은 자실체발생(子實體發生)의 변동(變動)을 조사(調査)하여 재배결과(栽培結果)의 조기예측(早期豫測)을 위한 회귀식(回歸式)을 산출(算出)하였다.

• 한국균학회지
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• 제49권3호
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• pp.425-425
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• 2021

• 한국식품저장유통학회지
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• 제24권3호
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• pp.381-386
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• 2017

Ergocalciferol은 vitamin D의 활성을 가지는 것으로 알려져 있다. 본 연구에서는 팽이(Flammulina velutipes), 느타리 (Pleurotus ostreatus), 애느타리(Pleurotus ostreatus), 새송이(Pleurotus eryngii), 양송이(Agaricus bisporus), 표고(Lentinula edodes), 목이(Auricularia auricula-judae) 등 7종의 버섯을 대상으로 자외선 조사에 따른 ergocalciferol(EC, 비타민 $D_2$) 함량의 증진 효과를 조사하였다. 자외선을 조사하지 않은 버섯의 ergocalciferol 함량은 극히 낮았으나 자외선 조사에 의해 ergocalciferol 함량이 크게 증가하였으며, 자외선 A, B, C 중 특히 자외선 B가 EC 함량 증진에 효과적이었다. 버섯에서 ergocalciferol의 생성은 자외선에 직접 노출되는 부위 즉 깊이 1 mm 이내의 버섯 표면에서만 일어나는 것으로 조사되었다. 따라서 세절하지 않은 생 버섯에 자외선을 조사할 경우 팽이 버섯과 같이 체적에 비해 표면적인 넓은 종류의 버섯에서 ergocalciferol 생성이 많았고, 건조 및 세절할 경우에는 EC 생성의 기질인 ergosterol의 함량이 높은 버섯에서 ergocalciferol 생성이 많았는데, 새송이, 표고, 양송이 등이 이에 속하였다.

• 한국버섯학회지
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• 제16권3호
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• pp.218-224
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• 2018

An auxin-producing bacterium, Klebsiella michiganensis Jopap-1, was isolated from a button mushroom bed in Buyeo-Gun, Chungcheongnam-Do. The strain Jopap-1 was classified as a novel strain of K. michiganensis based on a chemotaxonomic and phylogenetic analysis. The isolated K. michiganensis Jopap-1 was confirmed to produce indole-3-acetic acid (IAA), which is one of auxin hormones by TLC and HPLC analyses. The maximum concentration of IAA ($96.05mg\;L^{-1}$) was detected in the culture broth incubated in R2A medium containing 0.1% L-tryptophan for 48 h at $35^{\circ}C$ by HPLC quantity analysis. A negative relationship between IAA production and pH variation was estimated to show that the increase of IAA caused acidic pH in the culture. The effect of the supplement on L-tryptophan (precursor of IAA) production was observed to be highest at 0.1% concentration, but was significantly lowered above a concentration of 0.2%. To investigate the growth-promoting effects on the crops, the culture broth of E. michiganensis Jopap-1 was infected to water cultures and seed pots of mung bean and lettuce. Consequently, the adventitious root induction and root growth of mung bean and lettuce were observed to be 2.1 and 1.8 times higher than those of the control.

• 한국버섯학회지
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• 제10권4호
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• pp.151-159
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• 2012

The button mushroom (Agaricus bisporus) is one of the most widely cultivated important edible mushroom species. In the breeding of new button mushroom, 'Seolgang' was developed by crossing two monokaryons 'CM020913-27' and 'SSU423-31'. Because of the secondarily homothallism, only a small percentage of the basidia produce 3 or 4 spores, which are mostly haploid (n) and do not fruit. Single spore cultures derived from these types of spores produce a vegetative mycelium that also contain a variable number of genetically identical nuclei per cell called monokaryon. The lack of clamp connections between monokaryon and dikaryon required a series of mycelial culture and fruiting test. After crossing, hybrids were cultivated on a small scale and on a commercial scale at a farm. For this, the spawn was made by a commercial spawn producer and the spawned compost by a commercial compost producer. Mycelial growth of 'Seolgang' on CDA was better at $20^{\circ}C$ and $25^{\circ}C$ when it was compared with that of '505 Ho'. The mature cap shape of new strain 'Seolgang' is oblate spheroid and the immature cap shape is round to oblate spheroid. The cap diameter was 41.2 mm on average. In comparison with white strain '505 Ho', the strain had a yield that was 9% higher. It produced fruiting bodies which had a higher weight on average per fruiting body and were 19% firmer with a good shelf life. Days of fruiting body were 3-4 days later than those of '505 Ho'. The physical characteristics such as elasticity, chewiness, adhesiveness were better than that of '505 Ho'. Genetic analysis of the new strain 'Seolgang' showed different profiles compared to '505 Ho', CM02913-27, SSU413-31, when RAPD primers A02 and O04 were used.