• Title/Summary/Keyword: Butanol

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Acetone-Butanol Fermentation of Rice Straw by Simultaneous Saccharification and Fermentation (동시당화 발효법에 의한 볏짚의 Acetone-Butanol 발효)

  • 권기석;전영숙;김병홍
    • Korean Journal of Microbiology
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    • v.26 no.3
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    • pp.278-282
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    • 1988
  • Rice straw was used in the acetone-butanol fermentation by semultaneous saccharification and fermentation (SSF) using Clostridium acetobutylicum and cellulolytic enzyme. Over 230 mM of solvent was produced from alkali treated rice straw of from ball-milled microcrystalline cellulose whilst only acidic fermentation products were formed from ball-milled rice straw. From the results it is concluded that rice straw used in the study contained an inhibitor for the solventogenesis by the organism which is insoluble in water and some organic solvent and destroyed by alkaline treatment.

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The investigation on the Ignition Delay of n-heptane/n-butanol Blend Fuel using a Rapid Compression Machine at Low Temperature Combustion Regime (저온연소조건에서 급속압축기를 이용한 n-heptane/n-butanol 혼합연료의 착화지연에 관한 연구)

  • Song, Jaehyeok;Kang, Kijoong;Yang, Zheng;Lu, Xingcai;Choi, Gyungmin;Kim, Duckjool
    • 한국연소학회:학술대회논문집
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    • 2013.06a
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    • pp.25-28
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    • 2013
  • This study presents both experimental and numerical investigation of ignition characteristics of n-heptane and n-butanol mixture. The $O_2$ concentration was fixed to 9-10% to make high exhaust gas recirculation(EGR) rate condition. Experiments were performed using a rapid compression machine. In addition, a numerical study of the ignition delay time was performed using CHEMKIN codes to validate experimental results and predict chemical species after combustion process. The results showed that the ignition delay time increased with increasing n-butanol ratio and the reactivity decreased by low $O_2$ concentration.

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Factos affecting the production of butanol and acetone by Clostridium acetobutylicum

  • Gottschalk, G.
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1986.12a
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    • pp.509.2-509
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    • 1986
  • Owing to the growing interest in the production of fuels and chemicals from biomass the well-know butanol-acetone fermentation as carried out by Clostridium acetobutylicum has been intensely studied again in recent years. Several solvent-yielding fermentation processes were established which are operated by using batch cultures or continuous cultures. 1 could be shown that under conditions of phosphate limitation an asporogenous mutant of C. acetobutylicum establishes itself in a chemostat which produces the solvents continuously. Attempts have been made to change the butanol/acetone ratio in favor of butanol production. A corresponding shift of the product spectrum can be achieved by carbon monoxide addition to the head space of the fermentation (B.H. Kim et al., App. Envioron. Microbiol. 48, 764-770 1984) or by iron limitation. Progress has been made in understanding the mechanism underlying the shift from acid to solvent prodcction. Experimental results are in agreement with the view that intracellular accumulation of acetic and butyric acid results in a shortage of phosphate and coenzyme A. This shortage may serve then as signal for the synthesis of the enzymes involved in the formation of acetone and butanol.

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약모밀(Houttuynia cordata) 뿌리에서 추출한 순차분획물의 항균활성

  • Kim, Min-Ju;Song, Jong-Ho;Gwon, Hyeok-Dong;Son, Jeong-Eun;Park, In-Ho
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.855-858
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    • 2001
  • Antimicrobial activities, extraction yields and chemical compositions of the extracts from the powder of vacuum dried Houttuynia cordata root on various solvents were investigated. Antimicrobial activity was investigated by disc diffusion method against several microorganisms, nine species of Gram positive and thirteen Gram negative bacteria. Ethanol, ethylacetate and butanol extracts exhibited potent antimicrobial activities against B. megaterium C. xerosis, S. aureus, S. epidermidis and E.coli. Chloroform and aqueous extracts from H. cordata showed weak antimicrobial activity against the tested bacteria. Ethylacetate and butanol extracts exhibited strong antimicrobial activity against the tested microorganisms. The extraction yields were 15.7%, 3.7%, 0.13%, 0.5% and 5.9% in ethanol, chloroform, ethylacetate, butanol and aqueous fractions, respectively. To further elucidate the effective components, solvent extracts was analyzed by GC/MSD.

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Separation Performance of Disc Plate and Frame Type Reverse Osmosis System (원판틀형 역삼투 시스템의 투과성능)

  • 노상호;이종일;김영채;배성렬;정건용
    • Membrane Journal
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    • v.10 no.3
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    • pp.155-162
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    • 2000
  • The reverse osmosis membrane housing(HY) was developed for excellent distribution of a feed solution, and the separation performance was compared with the commercialized Rochem(RC) system. The permeation flux of HY system was a little lower than that of RC system. On the other hand, the NaCl rejection ra4io was generally higher. Also, the permeation flux and rejection ratio for type A, B and C modules(disc plate and frame type) were measured using NaCl, sucrose and butanol solutions. The separation performance of type C module for NaCL and sucrose solutions was the most effective, and then those of type A and B were followed, respectively. However, the separation performance for butanol solution was type B, C and A order. The flux improvement ratio of type B to A increased as butanol concentration decreased or operating pressure increased up to 28 bar.

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The Effect of Water and Butanol of Solvent on the Synthesis of Polyisocyanurate in the Presence of Carboxylate Salt Catalyst (카르복실레이트 염 촉매를 사용한 폴리이소시아누레이트의 합성시 용매에 포함된 수분과 부탄올의 영향)

  • Lee, Suk-Jeong;Yang, Hyun-Soo;Choi, Kwang-Sik;Lee, Young-Chul
    • Applied Chemistry for Engineering
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    • v.3 no.4
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    • pp.588-594
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    • 1992
  • Polyisocyanurate has been obtained from toluene diisocyanate(TDI) in the presence of potassium octoate catalyst and the effects of water and butanol in solvent were studied for the determination of the change of viscosity, the reaction time (length of time required for 5 poise of viscosity of product), molecular dispersity of product, and TDI conversion. When butyl acetate contains 0.1% of water by weight, uretidione was formed and a higher conversion was obtained at the condition. The uretidione was not formed by adding butanol to the solvent. At a higher concentration of butanol, a higher TDI conversion and a wider molecular dispersity were obtained.

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Calculation and Measurement of Flash Point for n-Decane + n-Octanol and Acetic Acid + n-Butanol Using a Tag-Open-Cup Apparatus (Tag 개방식 장치를 활용한 n-Decane + n-Octanol계 및 Acetic Acid + n-Butanol계의 인화점 측정과 계산)

  • Ha, Dong-Myeong;Lee, Sungjin
    • Fire Science and Engineering
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    • v.29 no.6
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    • pp.45-50
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    • 2015
  • The flash point is one of the most important properties for characterizing the fire and explosion hazard of liquid solutions. In this study, a Tag open-cup apparatus was used to measure the flash points of two flammable binary mixtures, n-decane + n-octanol and acetic acid + n-butanol. The flash point temperature was estimated using the UNIFAC (Universal Functional Activity Coefficient) group contribution model and optimization method. The experimentally derived flash point was also compared with the predicted flash point. The two methods can estimate the flash point fairly well for the n-decane + n-octanol and acetic acid + n-butanol systems.

Effects of Five Fractions of Artemisia capillaris THUNB on $TGF{\beta}1-induced$ Apoptosis in HepG2 Cells (인진분획물이 인체간세포의 $TGF{\beta}1-induced$ Apoptosis에 미치는 영향)

  • 이지현;이장훈;우홍정
    • The Journal of Korean Medicine
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    • v.21 no.1
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    • pp.53-61
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    • 2000
  • Objectives: This study was camed out to examine the effect of five fractions of aqueous extract from Artemisia capillaris THUNB(ACT), on TGF, 1-induced apoptosis, cell viability, cell cycle progression and mRNA expression of apoptosis-related genes in human hepatocyte cell line HepG2. Methods: This study employed Tryphan blue exclusion assay, DNA fragmentation assay, Cpp32 protease activity assay and Quantitative RT-PCR analysis. Results: In the Tryphan blue exclusion assay, the butanol fraction of ACT with $TGF{\beta}$, l showed magnificent (Nice word, ut is it appropriate in a medical abstract\ulcorner) viability and the H2O fraction of ACT with $TGF{\beta}$, l also showed higher viability than only $TGF{\beta}$, l-treated group. DNA fragmentation assay showed that the butanol fraction and the H2O fraction carried inhibitory effects on apoptosis induction, with the butanol fraction displaying greater effects. The Cpp32 protease activity assay showed that the butanol fraction decreased Cpp32 protease activity. The H2O fraction of ACT had no significant effect on the Cpp32 protease activity. Quantitative RT-PCR showed that the butanol fraction suppressed Bax, p 15/INK4B, p21/Waf1, PAI-1 and increased Bcl-2 gene. Conclusions: The data shows that butanol fraction of ACT increases the hepatocyte viability and has the hepatocellular protective effect by the suppression of $TGF{\beta}$, l induced-apoptosis through gene regulation.

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Antioxidant Activities of n-Butanol Fraction of Pinus densiflora Siebold & Zucc. Needle in Caenorhabditis elegans. (솔잎 부탄올 분획의 예쁜꼬마선충 내의 항산화 효과)

  • Kim, Jun Hyeong;Kim, Na Yeong;Kwon, Kang Mu;Hwang, In Hyun;Kim, Dae Keun
    • Korean Journal of Pharmacognosy
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    • v.52 no.2
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    • pp.105-111
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    • 2021
  • To investigate the antioxidant activities of Pinus densiflora needle (Pinaceae), which is widely used as a traditional medicine in Korea, Caenorhabditis elegans model system was used. The n-butanol fraction of P. densiflora needle methanol extract showed the most potent DPPH radical scavenging and superoxide quenching activities in a dose-dependent manner. The n-butanol fraction was measured for the activities of superoxide dismutase (SOD), catalase, and oxidative stress tolerance by using C. elegans with reactive oxygen species (ROS) level. In addition, to see that the regulation of the stress response gene is responsible for the increased stress tolerance of C. elegans treated by the butanol extract, SOD-3 expression was measured using a transgenic strain. As a result, the P. densiflora needle n-butanol fraction increased SOD and catalase activities, and decreased ROS accumulation, dose-dependently. Furthermore, the n-butanol fraction-treated CF1553 worm showed higher SOD-3::GFP intensity than the control.

Flash Point Measurement of n-Propanol+n-Hexanol and n-Butanol+n-Hexanol Systems Using Seta Flash Closed Cup Tester (Seta Flash 밀폐식 장치를 이용한 n-Propanol+n-Hexanol계와 n-Butanol+n-Hexanol계의 인화점 측정)

  • Ha, Dong-Myeong;Lee, Sungjin
    • Journal of the Korean Society of Safety
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    • v.34 no.1
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    • pp.34-39
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    • 2019
  • Flash point is the important indicator to determine fire and explosion hazards of liquid solutions. In this study, flash points of n-propanol+n-hexanol and n-butanol+n-hexanol systems were obtained by Seta flash tester. The methods based on UNIFAC equation and multiple regression analysis were used to calculate flash point. The calculated flash point was compared with the experimental flash point. Absolute average errors of flash points calculated by UNIFAC equation are $2.9^{\circ}C$ and $0.6^{\circ}C$ for n-propanol+n-hexanol and n-butanol+n-hexanol, respectively. Absolute average errors of flash points calculated by multiple regression analysis are $0.5^{\circ}C$ and $0.2^{\circ}C$ for n-propanol+ n-hexanol and n-butanol+n-hexanol, respectively. As can be seen from AAE, the values calculated by multiple regression analysis are noticed to be better than the values by the method based on UNIFAC eauation.