• Macromolecular Research
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• v.11 no.5
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• pp.334-340
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• 2003

In order to fabricate new sustained delivery device of nerve growth factor (NGF), we developed NGF-loaded biodegradable poly(L-lactide-co-glycolide) (PLGA, the mole ratio of lactide to glycolide 75:25, molecular weight: 83,000 and 43,000 g/mole, respectively) film by novel and simple sandwich solvent casting method for the possibility of the application of neural tissue engineering. PLGA was copolymerized by direct condensation reaction and the molecular weight was controlled by reaction time. Released behavior of NGF from NGF-loaded films was characterized by enzyme linked immunosorbent assay (ELISA) and degradation characteristics were observed by scanning electron microscopy (SEM) and gel permeation chromatography (GPC). The bioactivity of released NGF was identified using a rat pheochromocytoma (PC-12) cell based bioassay. The release of NGF from the NGF-loaded PLGA films was prolonged over 35 days with zero-order rate of 0.5-0.8 ng NGF/day without initial burst and could be controlled by the variations of molecular weight and NGF loading amount. After 7 days NGF released in phosphate buffered saline and PC-12 cell cultured on the NGF-loaded PLGA film for 3 days. The released NGF stimulated neurite sprouting in cultured PC-12 cells, that is to say, the remained NGF in the NGF/PLGA film at 37 $^{\circ}C$ for 7 days was still bioactive. This study suggested that NGF-loaded PLGA sandwich film is released the desired period in delivery system and useful neuronal growth culture as nerve contact guidance tube for the application of neural tissue engineering.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.6
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• pp.147-153
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• 2018

PLGA microcapsule particles encapsulating BSA aqueous solutions were prepared using a water-in-oil-in-water emulsion method. The morphology, particle size, BSA encapsulating efficiency, and in-vitro release test were also studied using the microcapsule particles. In the outer aqueous phase, an emulsifier, e.g., PVA, was replaced with metal salts for surface solidification. Scanning electron microscopy (SEM) showed that the microcapsule particles had smooth surfaces and were between $1{\mu}m$ and $7{\mu}m$ in size. The microcapsule particle morphology was affected directly by the ratio between the polymer solution and inner aqueous solution, and composition of the outer aqueous solution. The factors also partially affected the BSA encapsulation efficiencies and in-vitro release rates. All the microcapsule particles showed an initial burst release through the in-vitro release test. On the other hand, the particles also showed a relatively long release period. Metal salts could be good choices to replace the emulsifier to solidify the microcapsule particle surfaces.

• MALSORI
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• no.56
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• pp.49-73
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• 2005

This study explores the most important temporal cues in the perception of the voiced/voiceless distinction of English plosives in terms of newly defined measures of perception: original signal to response agreement, unit signal to response agreement, and robustness. Seven native speakers of English and three leveled groups of Korean English learners participated in the present study. The results showed that both native speakers of English and Korean groups failed to successfully perceive the voiced/voiceless distinction of English plosives, particularly alveolar plosives, in word-medial trochaic positions. The results also showed that in word-initial and word-medial iambic positions both native speakers of English and Korean groups employ the information in the release burst and aspiration in the perception of the voiced/voiceless distinction, of English plosives, and that in word-final positions native speakers of English employ the information in the preceding vowel, while Korean groups employ the information in the closure interval.

• Journal of the Korean Society of Combustion
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• v.23 no.1
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• pp.36-43
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• 2018

Numerical simulations and experiments are performed to investigate the flame development inside tubes with different diameters at the same burst pressure. It is shown that generation of a stable flame play a role in self-ignition. In the smaller tube, multi-dimensional shock interaction is occurred near the diaphragm. After flame of a cross-section is developed, stable flame remains for a moment then it grows having enough energy to overcome the sudden release at the exit. Whereas shock interaction generate complex flow further downstream for a larger tube, it results in stretched flame. This dispersed flame has lower average temperature which makes it easily extinguished.

• 한국연소학회:학술대회논문집
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• 2012.04a
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• pp.245-246
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• 2012

The possibility that self-ignition can be generated near an obstacle is high in practical applications such as a hydrogen car. In this paper, experimental investigations were suggested to understand the effects of a wall on self-ignition phenomena through high-speed images. The results showed that the existence of a wall could not change the ignition phenomena itself irrespective of wall height and burst pressure. However, when a strong flame was induced in the tube, a wall could promote the flame stabilization.

• Applied Chemistry for Engineering
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• v.1 no.2
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• pp.147-153
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• 1990

Poly(hydroxypropyl methacrylate) membranes and matrix devices having tertiary amine moiety were prepared by sintering method, and their permeation characteristics were investigated. The water content of this membrane was increased with decreasing the pH of the medium. The permeability of this membrane was increased with decreasing the sintering pressure. Using sintered matrix device 'burst effect' was found at the early stage, and initial insulin release of glucose oxidase immobilized matrix device could be controlled by glucose concentration of the solution.

• Journal of Pharmaceutical Investigation
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• v.19 no.2
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• pp.87-92
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• 1989

The multilayered monolithic type transdermal delivery device has been designed and analyzed by a numerical analysis. The device consists of three layered polymer matrices which posess the different diffusion parameters, respectively. The purpose of this study was to design an ideal transdermal drug delivery device which is capable of initial burst and zero order release later on. Numerical modelings were simulated for a dispersed and a dissolved multilayered monolithic system. The results showed that the dispersed multilayered monolithic system could meet the requirements for an ideal transdermal delivery device.

• Archives of Pharmacal Research
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• v.26 no.8
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• pp.649-652
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• 2003

Polymeric nanoparticles composed of polystyrene (PS) as core and poly(methacrylic acid) (PMA) as corona were prepared by the dispersion copolymerization. The potential of the nanoparticles as carriers for recombinant human epidermal growth factor (EGF) was investigated. The nanoparticles showed monodispersity and good water-dispersibility. The loading content of EGF to the nanoparticles was very high due to electrostatic interaction between EGF and nanoparticles. EGF was released as a pseudo-zero order pattern after initial burst effect. The nanoparticles were sufficient for A431 cells proliferation.

• The Korean Journal of Pharmacology
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• v.31 no.3
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• pp.333-344
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• 1995

The effects of adenosine and $N^6-cyclopentyladenosine$ (CPA) on superoxide production, myeloperoxidase release and $Ca^{2+}$ mobilization stimulated by fMLP in neutrophils were investigated. The effects were also observed on the stimulatory actions of C5a and PMA and the responses in lipopolysaccharide-primed neutrophils. In addition, the involvement of cAMP in the inhibitory action of adenosine was examined. The fMLP-stimulated neutrophil respiratory burst, degranulation and intracellular $Ca^{2+}$ mobilization may be regulated by activation of adenosine receptors. Adenosine may not affect the stimulated neutrophil responses due to activation of protein kinase C. fMLP-stimulated respiratory burst in lipopolysaccharide-primed neutrophils may be less sensitive to adenosine, compared with nonprimed cells. The inhibitory effect of theophylline in the presence of adenosine on neutrophil responses appears to be ascribed to accumulation of intracellular cAMP.

• Proceedings of the KSPS conference
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• 1996.10a
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• pp.390-398
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• 1996

Acoustic analysis study was performed on 20 normal subjects by speaking nonsense syllables composed of Korean bilabial stops(/p, $p^{*}$/, ph/) and their Preceding and/or following vowel /a/(that is, [pa, $p^{*}a$, pha, apa, $ap^{*}a$, apha]) with an ultraminiature pressure sensor in their mouths. Speech materials were phonated twice, once with a moderate voice, another time with a loud voice. The acoustic signal and intraoral pressure were recorded simultaneously on computer. By these procedures, we were to measure the intraoral pressure, closure duration and VOT of Korean bilabial stops, and to compare the values one another according to the intensity of phonation and the position of the target consonants. Intraoral pressure was measured by the peak intraoral pressure value of its wave; closure duration by the time interval between the onset of intraoral pressure build-up and the burst meaning the release of closure; Voice onset time(VOT) by the time interval between the burst and the onset of glottal vibration. Heavily aspirated bilabial stop consonant /ph/ showed the highest intraoral pressure value, unaspirated /p$^{*}$/, the second, slightly aspirated /p/, the lowest. The syllable initial bilabial stops showed higher intraoral pressure than word initial stops, and the value of loudly phonated consonants were higher than moderate consonants. The longest closure duration period was that of /$p^{*}$/ and the shortest, /p/, and the duration was longer in word initial position and in the moderate voice. In VOT, the order of the longest to shortest was /ph/, /p/, /$p^{*}$/, and the value was shorter when the consonant was in intervocalic position and when it was phonated with a loud voice.