• Journal of Environmental Science International
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• v.16 no.6
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• pp.751-761
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• 2007

This study is carried out through ecological situation of native Bupleurum latissimum habitats and the research of environmental conditions for conservation of native sites, restoration of alternative habitats and preliminary data for application of useful medicinal plant. Native Bupleurum latissimum habitats were distributed on lower and middle part of the mountain from Altitude 54m to 185m, and the directions of slope were researched as usually northen and western slope. As a result of phytosociological research in 12 stands of native Bupleurum latissimum habitats, they were seperated to two communities(Bupleurum latissimum typical community and Bupleurum latissimum-Galium spurium community) in accordance with containable rate of the organic matter and soil hardeness. Soil condition is usually constituted as loamy sand and clay loam. Bupleurum latissimum is propagated in valuable soil conditions. Bupleurum latissimum lives with Morus bombycis, Reynoutria sachalinensis in Bupleurum latissimum typical community and Thalictrum aquilegifolium, Stellaria media, Hedera rhombea etc. is appeared with Bupleurum latissimum in Bupleurum latissimum-Galium spurium community. Especially, companion species were researched as Aster glehni, Dystaenia takeshimana and so on. The flora of vascular plants in this study is 44 families, 88genus 82species, 2 subspecies, 14 varietas, 4 forma and total 102 taxa. Plants of compositae occupied 10.78% among whole of them.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.9 no.6
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• pp.78-85
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• 2006

This study was to analyze vegetation and soil characteristic, and ordination of Bupleurum latissimum community located in Seo-Myun, Isl. Ulleungdo. The Bupleurum latissimum community was classified into Physocarpus insularis subcommunity and Artemisia stolonifera subcommunity, and there was Artemisia stolonifera subcommunity in destroyed site of Bupleurum latissimum community. In the study sites, soil organic matter, nitrogen, available phosphorous, changeable potassium, changeable calcium, changeable magnesium concentration, cation exchangeable capacity, and soil pH were ranged from 19.0~25.6%, 0.67~0.96%, 47.8~103.0ppm, 2.4~2.8(me/100g), 13.8~15.0(me/100g), 8.4~9.0 (me/100g), 34.0~38.4(me/100g), and 5.9~6.0, respectively. The Bupleurum latissimum community had higher nitrogen, exchangeable magnesium, and available phosphorous concentration than in Bupleurum euphorbioides and Bupleurum longiradiatum community. The Berberis amurensis var. latifolia and Valeriana officinalis var. latifolia that was companion species of Bupleurum latissimum community was found in area of having high soil organic matter and nitrogen concentration. The Ligustrum foliosumwas found in shrubbery, and so the reason could give little competition with Bupleurum latissimum community. The Bupleurum latissimum is the endangered species managed by Ministry of Environments in Korea. Its community has been destroyed, and so we need any action to protect the community.

• Korean Journal of Plant Taxonomy
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• v.38 no.1
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• pp.43-50
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• 2008

The pollination system of Bupleurum latissimum Nakai (Apiaceae) was investigated in the natural population of Korea. The various insects of 19 species, 11 families, 5 orders visited the flowers of B. latissimum. Episyrphus balteatus and Lasioglossum occidens were considered as the most effective pollen vector which have associated specially with B. latissimum. The visitation frequency peaked at 10 AM - 13 PM and no visitor was recognized during night time. The flowers of B. latissimum last during only three days and they are protandry. It is also confirmed that the flower of B. latissimum is self-compatible and cross-pollination by vectors is critical for successful seed setting.

• Korean Journal of Medicinal Crop Science
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• v.14 no.6
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• pp.342-346
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• 2006

Anatomical characters of the Bupleurum latissimum Nakai, an endemic species of Korea, were investigated to confirm its phylogenetic relationships. Compare to other species with anatomical characters, B. latissimum is very similar with B, euphorbioides and B, longeradiatum in point of lacking of pith in the stem, shape of involucres, number of vascular bundles in radical leaf and cauline leaf, and lacking stomata in adaxial leaf surface. The other hand, protruded pollen aperture character appears in B. latissimum and B. euphorbioides. On the based of anatomical characters, therefore, B. latissimum has closest relationships with B. euphorbioides and B. longeradiatum. It also needs molecular study including Asian species in order to confirm phylogenetic position and speciation process apparently.

• Korean Journal of Plant Taxonomy
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• v.38 no.1
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• pp.31-42
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• 2008

It was revaluated about taxonomic position of Bupleurum latissimum Nakai (Apiaceae) by the cluster analysis, the principal component analysis, and the discriminant analysis. Its diagnostic characteristics include bracteole length longer than umbellules, 16-21 pedicel numbers, plant with evergreen leaves, chromosome number 2n = 16 and cauline leaves with auriculate base. It is morphologically similar to B. euphorbioides in having ovate bracteoles, 16-21 pedicel numbers, chromosome number 2n = 16, and cauline leaves with auriculate base, but distinguished from B. longiradiatum with having linear bracteoles, 7-15 pedicel numbers, and chromosome number 2n = 12. Morphological characters support that Bupleurum latissimum Nakai is a Korean endemic species.

• Animal cells and systems
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• v.8 no.4
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• pp.289-294
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• 2004

Bupleurum latissimum is a narrowly endemic and endangered plant, restricted to only two small populations on steep cliffs of a small island, Ulleung Island, in Korea. The genetic diversity and population differentiation in the two remnant populations of the species were investigated using RAPD (random amplified polymorphic DNA) analysis. The Neis gene diversities were 0.146 in the smaller population of 45 individuals, and 0.151 in the larger population of 61 individuals. The genetic variation was not significantly different between these two populations. Genetic diversity within populations was not low considering the very small size of populations. Analysis of molecular variance (AMOVA) revealed higher variation within populations (65.9%) than genetic differentiation between them (34.1%). B. latissimum revealed higher population differentiation than other outbreeding species. The differentiation of the populations corresponded to low gene flow (Nem = 0.482). The cluster and principal coordination analyses provide strong support for high population differentiation, showing that all individuals of the two populations have built up population-specific clusters. Although gene flow between the two populations of B. latissimum was limited, they have preserved relatively high levels of genetic variation.

• Korean Journal of Medicinal Crop Science
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• v.14 no.6
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• pp.354-359
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• 2006

Chromosome analysis using mitosis, meiosis and bicolor FISH were carried out in Bupleurum latissimum Nakai, which is one of the endemic plants in Ulleung island of korea. The somatic methaphase chromosomes number of this plant was 2n = 2x = 16 and the chromosome complements consisted of six pairs of metacentrics and two pairs of submetacentrics. The size of chromosomes ranged 2.40${\sim}$4.20 ${\mu}$m and NOR (nucleolus organizer region) chromosome did not observed using conventional staining. In meiosis chromosomes, metaphase-I and anaphase-I were observed. Metaphase-I anaphase-I showed 8 bivalents and chromosomes migration to make two daughter cells. Using bicolor FISH, one pair of 5S and 45S rDNA signals were detected on the centromeric region of chromosome 3 and the end of short of chromosome 2,respectively. We also observed the NOR using 45S rDNA probe.

• The Korea Journal of Herbology
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• v.24 no.3
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• pp.59-68
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• 2009

Objectives : Bupleuri Radix (Siho) is prescribed as the root of different Bupleurum species on the pharmarcopoeia in Korea and China. Moreover, other species and varieties of the genus Bupleurum have been also distributed on the herbal market as Bupleuri Radix. However, due to the morphological similarity and frequent occurrence of intermediate forms, the correct identification of this radix is very difficult. To develop a reliable method for correct identification and improving the quality standards of official Bupleuri Radix, we analyzed sequences of the ribosomal RNA gene and internal transcribed spacer (rDNA-ITS) region. Methods : PCR amplification of rDNA-ITS region was performed using ITS1 and ITS4 primer from 6 Bupleurum species and 1 variety, B. falcatum L. (Siho), an improved breed of B. falcatum L. (Samdo-Siho), B. chinense DC. (Buk-Siho), B. scorzonerifolium Willd. (Nam-Siho), B. longiadiatum Turcz. (Gae-Siho), B. euphorbiodes Nakai (Deungdae-Siho) and B. latissimum Nakai (Seom-Siho), and nucleotide sequence was determined after sub-cloning into the pGEM-Teasy vector. Authentic marker nucleotides were estimated by the analysis of ClastalW using entire rDNA-ITS sequence of three samples per species. Results : In comparative analysis of the rDNA-ITS sequences, we found specific nucleotides to distinguish Korean (B. falcatum L. and its variety) and Chinese official species (B. chinense DC. and B. scorzonerifolium Willd.) from others at positions 411 and 447, and positions 89, 101, 415 and 599, respectively. Futhermore, we also found nucleotide indels (insertion and/or deletion) and substitutions to identify each of different Bupleurum species, 2 positions for B. falcatum L. and its variety, 6 positions for B. chinense DC., 49 positions for B. scorzonerifolium Willd., 8 positions for B. euphorbioides Nakai, 7 positions for B. longiradiatum Nakai and 9 positions for B. latissimum Nakai. These sequence differences at corresponding positions are avaliable nucleotide markers to determine the botanical origins of Bupleuri Radix. Moreover, we confirmed the phylogenetic relationship of B. latissimum Nakai, a Korean endemic speices, among Bupleurum species based on the rDNA-ITS sequence. Conclusions : These marker nucleotides would be useful to identify the official herbal medicines by the providing of definitive information that can identify each plant species and distinguish it from unauthentic adulterant Bupleurum species.

• Proceedings of the Korean Society of Environment and Ecology Conference
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• 2010.04a
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• pp.52-54
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• 2010

• Korean Journal of Plant Resources
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• v.20 no.4
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• pp.367-374
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• 2007

This study was carried out to establish the micropropagation system of Bupleurum latissimum Nakai that is a Korean native endangered species. Callus were induced from the leaf, petiole and floral bud and the percentage of callus formation was highest in the floral bud on the MS medium containing 2.0 mg ${\cdot}$ $L^{-1}$ 2,4-D. Especially, callus induced from floral bud was formed 77.8% and the percentage of shoot formation was 42.6% on the MS medium containing 2.0 mg ${\cdot}$ $L^{-1}$ 2,4-D plus 1.0 mg ${\cdot}$ $L^{-1}$ TDZ. For simultaneously callus formation and shoot regeneration, 1/2 MS medium was more effective than MS medium. The percentage callus formation, shoot regeneration and rooting were 46.3%, 13.0%, 13.0% in 1/2 MS medium, respectively. Soot regeneration from callus was good in 1/2 MS medium supplemented with 2.0 mg ${\cdot}$ $L^{-1}$ 2,4-D plus 1.0 mg ${\cdot}$ $L^{-1}$ BA where percentage of shoot regeneration was 74.1 %, and the number of shoot per explant was 2.4. The percentage of rooting was lowest (57.8%) in control while it was highest (97.8%) in 1.5 mg ${\cdot}$ $L^{-1}$ NAA. In acclimatization of regenerated plantlets, the percentage of survived plantlets was highest (86.1%), and plant height, root length and fresh weight were good in the soil for horticulture.