• 한국환경과학회지
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• 제16권6호
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• pp.751-761
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• 2007

This study is carried out through ecological situation of native Bupleurum latissimum habitats and the research of environmental conditions for conservation of native sites, restoration of alternative habitats and preliminary data for application of useful medicinal plant. Native Bupleurum latissimum habitats were distributed on lower and middle part of the mountain from Altitude 54m to 185m, and the directions of slope were researched as usually northen and western slope. As a result of phytosociological research in 12 stands of native Bupleurum latissimum habitats, they were seperated to two communities(Bupleurum latissimum typical community and Bupleurum latissimum-Galium spurium community) in accordance with containable rate of the organic matter and soil hardeness. Soil condition is usually constituted as loamy sand and clay loam. Bupleurum latissimum is propagated in valuable soil conditions. Bupleurum latissimum lives with Morus bombycis, Reynoutria sachalinensis in Bupleurum latissimum typical community and Thalictrum aquilegifolium, Stellaria media, Hedera rhombea etc. is appeared with Bupleurum latissimum in Bupleurum latissimum-Galium spurium community. Especially, companion species were researched as Aster glehni, Dystaenia takeshimana and so on. The flora of vascular plants in this study is 44 families, 88genus 82species, 2 subspecies, 14 varietas, 4 forma and total 102 taxa. Plants of compositae occupied 10.78% among whole of them.

• 한국환경복원기술학회지
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• 제9권6호
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• pp.78-85
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• 2006

This study was to analyze vegetation and soil characteristic, and ordination of Bupleurum latissimum community located in Seo-Myun, Isl. Ulleungdo. The Bupleurum latissimum community was classified into Physocarpus insularis subcommunity and Artemisia stolonifera subcommunity, and there was Artemisia stolonifera subcommunity in destroyed site of Bupleurum latissimum community. In the study sites, soil organic matter, nitrogen, available phosphorous, changeable potassium, changeable calcium, changeable magnesium concentration, cation exchangeable capacity, and soil pH were ranged from 19.0~25.6%, 0.67~0.96%, 47.8~103.0ppm, 2.4~2.8(me/100g), 13.8~15.0(me/100g), 8.4~9.0 (me/100g), 34.0~38.4(me/100g), and 5.9~6.0, respectively. The Bupleurum latissimum community had higher nitrogen, exchangeable magnesium, and available phosphorous concentration than in Bupleurum euphorbioides and Bupleurum longiradiatum community. The Berberis amurensis var. latifolia and Valeriana officinalis var. latifolia that was companion species of Bupleurum latissimum community was found in area of having high soil organic matter and nitrogen concentration. The Ligustrum foliosumwas found in shrubbery, and so the reason could give little competition with Bupleurum latissimum community. The Bupleurum latissimum is the endangered species managed by Ministry of Environments in Korea. Its community has been destroyed, and so we need any action to protect the community.

• 식물분류학회지
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• 제38권1호
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• pp.43-50
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• 2008

환경부 멸종위기종인 섬시호(Bupleurum latissimum)를 현지 내에서 보다 효과적으로 보존할 수 있도록 종합적인 종생물학적 정보를 수집하고자, 섬시호의 수분기작 및 수분매개자의 특성을 조사하였다. 섬시호는 5월 말에 개화하며, 꽃은 개화된 상태로 3일간 지속되었다. 꽃은 웅예선숙으로 수술이 성숙한 개화 1-2일째에 암술은 작은 돌기 형태를 이루었다. 꽃이 지기 시작하는 3일째부터 화주가 성숙하여 길게 나오고, 꽃잎은 노란색에서 붉은색으로 변하며 탈락하였다. 섬시호를 방문하는 곤충은 총 5목 11과 19종이었으며, 그 중 가장 효율적이며 주된 수분매개자는 호리꽃등에(Episyrphus balteatus)와 흰줄꼬마꽃벌(Lasioglossum occidens)이다.

• 한국약용작물학회지
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• 제14권6호
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• pp.342-346
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• 2006

Anatomical characters of the Bupleurum latissimum Nakai, an endemic species of Korea, were investigated to confirm its phylogenetic relationships. Compare to other species with anatomical characters, B. latissimum is very similar with B, euphorbioides and B, longeradiatum in point of lacking of pith in the stem, shape of involucres, number of vascular bundles in radical leaf and cauline leaf, and lacking stomata in adaxial leaf surface. The other hand, protruded pollen aperture character appears in B. latissimum and B. euphorbioides. On the based of anatomical characters, therefore, B. latissimum has closest relationships with B. euphorbioides and B. longeradiatum. It also needs molecular study including Asian species in order to confirm phylogenetic position and speciation process apparently.

• 식물분류학회지
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• 제38권1호
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• pp.31-42
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• 2008

환경부 지정 멸종위기식물이며 한국특산식물인 섬시호(Bupleurum latissimum Nakai)의 외부형태학적인 유연관계를 재조사하기 위해 유집분석과 주성분분석 그리고 판별분석을 수행하였다. 섬시호는 경생엽이 이저이고 소총포가 꽃보다 길고 난형이며 소화경수가 16-25개인 점에서 등대시호와 유사하나, 식물체가 대형이고 상록성 2년생 식물이며 소총포가 녹색인 점에서 뚜렷이 구별되었다. 또한 섬시호는 경생엽이 이저이고 식물체가 대형이며 소총포가 녹색인 점에서 개시호와 유사하나 소총포가 꽃보다 짧고 선형이며 소화경수가 7-15개인 개시호와 구별되었다. 따라서 수리분류학적 여러 형질들은 섬시호가 한국산 나머지 시호속 종들과 뚜렷이 구별되나 등대시호와 유연관계가 깊다는 사실을 지지해 주었다.

• Animal cells and systems
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• 제8권4호
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• pp.289-294
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• 2004

Bupleurum latissimum is a narrowly endemic and endangered plant, restricted to only two small populations on steep cliffs of a small island, Ulleung Island, in Korea. The genetic diversity and population differentiation in the two remnant populations of the species were investigated using RAPD (random amplified polymorphic DNA) analysis. The Neis gene diversities were 0.146 in the smaller population of 45 individuals, and 0.151 in the larger population of 61 individuals. The genetic variation was not significantly different between these two populations. Genetic diversity within populations was not low considering the very small size of populations. Analysis of molecular variance (AMOVA) revealed higher variation within populations (65.9%) than genetic differentiation between them (34.1%). B. latissimum revealed higher population differentiation than other outbreeding species. The differentiation of the populations corresponded to low gene flow (Nem = 0.482). The cluster and principal coordination analyses provide strong support for high population differentiation, showing that all individuals of the two populations have built up population-specific clusters. Although gene flow between the two populations of B. latissimum was limited, they have preserved relatively high levels of genetic variation.

• 한국약용작물학회지
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• 제14권6호
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• pp.354-359
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• 2006

Chromosome analysis using mitosis, meiosis and bicolor FISH were carried out in Bupleurum latissimum Nakai, which is one of the endemic plants in Ulleung island of korea. The somatic methaphase chromosomes number of this plant was 2n = 2x = 16 and the chromosome complements consisted of six pairs of metacentrics and two pairs of submetacentrics. The size of chromosomes ranged 2.40${\sim}$4.20 ${\mu}$m and NOR (nucleolus organizer region) chromosome did not observed using conventional staining. In meiosis chromosomes, metaphase-I and anaphase-I were observed. Metaphase-I anaphase-I showed 8 bivalents and chromosomes migration to make two daughter cells. Using bicolor FISH, one pair of 5S and 45S rDNA signals were detected on the centromeric region of chromosome 3 and the end of short of chromosome 2,respectively. We also observed the NOR using 45S rDNA probe.

• 대한본초학회지
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• 제24권3호
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• pp.59-68
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• 2009

Objectives : Bupleuri Radix (Siho) is prescribed as the root of different Bupleurum species on the pharmarcopoeia in Korea and China. Moreover, other species and varieties of the genus Bupleurum have been also distributed on the herbal market as Bupleuri Radix. However, due to the morphological similarity and frequent occurrence of intermediate forms, the correct identification of this radix is very difficult. To develop a reliable method for correct identification and improving the quality standards of official Bupleuri Radix, we analyzed sequences of the ribosomal RNA gene and internal transcribed spacer (rDNA-ITS) region. Methods : PCR amplification of rDNA-ITS region was performed using ITS1 and ITS4 primer from 6 Bupleurum species and 1 variety, B. falcatum L. (Siho), an improved breed of B. falcatum L. (Samdo-Siho), B. chinense DC. (Buk-Siho), B. scorzonerifolium Willd. (Nam-Siho), B. longiadiatum Turcz. (Gae-Siho), B. euphorbiodes Nakai (Deungdae-Siho) and B. latissimum Nakai (Seom-Siho), and nucleotide sequence was determined after sub-cloning into the pGEM-Teasy vector. Authentic marker nucleotides were estimated by the analysis of ClastalW using entire rDNA-ITS sequence of three samples per species. Results : In comparative analysis of the rDNA-ITS sequences, we found specific nucleotides to distinguish Korean (B. falcatum L. and its variety) and Chinese official species (B. chinense DC. and B. scorzonerifolium Willd.) from others at positions 411 and 447, and positions 89, 101, 415 and 599, respectively. Futhermore, we also found nucleotide indels (insertion and/or deletion) and substitutions to identify each of different Bupleurum species, 2 positions for B. falcatum L. and its variety, 6 positions for B. chinense DC., 49 positions for B. scorzonerifolium Willd., 8 positions for B. euphorbioides Nakai, 7 positions for B. longiradiatum Nakai and 9 positions for B. latissimum Nakai. These sequence differences at corresponding positions are avaliable nucleotide markers to determine the botanical origins of Bupleuri Radix. Moreover, we confirmed the phylogenetic relationship of B. latissimum Nakai, a Korean endemic speices, among Bupleurum species based on the rDNA-ITS sequence. Conclusions : These marker nucleotides would be useful to identify the official herbal medicines by the providing of definitive information that can identify each plant species and distinguish it from unauthentic adulterant Bupleurum species.

• 한국환경생태학회:학술대회논문집
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• 한국환경생태학회 2010년 학술대회 논문집
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• pp.52-54
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• 2010

• 한국자원식물학회지
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• 제20권4호
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• pp.367-374
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• 2007

엽, 엽병과 화뢰 등의 치상 절편체 부위 모두에서 캘러스가 형성되었으며 특히 화뢰에서 캘러스 형성률이 71.6%로 가장 높았다. 화뢰배양시 2.0mg ${\cdot}$ $L^{-1}$ 2,4-D와 1.0mg ${\cdot}$ $L^{-1}$ TDZ의 혼용처리구에서 캘러스 형성이 77.8%로 양호했으며 신초 재분화율 역시 42.6%를 보였다. 캘러스 형성과 신초 재분화를 동시에 시킬 때 1/2 MS배지가 효과적이었는데 1/2 MS배지에서 캘러스 형성률은 46.3%, 신초 재분화율은 13.0%,그리고 발근율은 13.0%를 보였다. 캘러스를 통한 신초의 재분화를 위해 1/2 MS배지에 2.0mg ${\cdot}$ $L^{-1}$ 2,4-D와 1.0mg ${\cdot}$ $L^{-1}$ BA를 첨가했을 때 신초 재분화율은 74.1%를 보였고 절편체당 신초수는 2.4개로 좋았다. 발근율은 대조구에서 57.8%로 낮았으나 1.5mg ${\cdot}$ $L^{-1}$ NAA 첨가된 배지에서 97.8%로 높았다. 재분화된 기내 식물체를 원예용 상토에 순화했을 때 생존률이 86.1%로 높았으며 초장, 근장, 생체중 역시 양호하였다.