• Journal of Pharmaceutical Investigation
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• v.25 no.3
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• pp.7-20
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• 1995

Taurine, a ${\beta}-amino$ acid, plays an important role as a neuromodulator and is necessary for the normal development of the brain. Since de novo synthesis of taurine in the brain is minimal and in vivo studies suggest that taurine dose not cross the blood-brain barrier, we examined whether the choroid plexus, the blood-cerebrospinal fluid (CSF) barrier, plays a role in taurine transport in the central nervous system. The uptake of $[^3H]-taurine$ into ATP depleted choroid plexus from rabbit was substantially greater in the presence of an inwardly directed $Na^+$ gradient taurine accumulation was negligible. A transient in side-negative potential gradient enhanced the $Na^+-driven$ uptake of taurine into the tissue slices, suggesting that the transport process is electrogenic, $Na^+-driven$ taurine uptake was saturable with an estimated $V_{max}$ of $111\;{\pm}\;20.2\;nmole/g/15\;min$ and a $K_M\;of\;99.8{\pm}29.9\;{\mu}M$. The estimated coupling ratio of $Na^+$ and taurine was $1.80\;{\pm}\;0.122.$ $Na^+-dependent$ taurine uptake was significantly inhibited by ${\beta}-amino$ acids, but not by ${\alpha}-amino$ acids, indicating that the transporter is selective for ${\beta}-amino$ acids. Since it is known that the physiological concentration of taurine in the CSF is lower than that in the plasma, the active transport system we characterized may face the brush border (i.e., CSF facing) side of the choroid plexus and actively transport taurine out of the CSF. Therefore, we examined in vivo elimination of taurine from the CSF in the rat to determine whether elimination kinetics of taurine from the CSF is consistent with the in vitro study. Using a stereotaxic device, cannulaes were placed into the lateral ventricle and the cisterna magna of the rat. Radio-labelled taurine and inulin (a marker of CSF flow) were injected into the lateral ventricle, and the concentrations of the labelled compounds in the CSF were monitored for upto 3 hrs in the cisterna magna. The apparent clearance of taurine from CSF was greater than the estimated CSF flow (p<0.005) indicating that there is a clearance process in addition to the CSF flow. Taurine distribution into the choroid plexus was at least 10 fold higher than that found in other brain areas (e. g., cerebellum, olfactory bulb and cortex). When unlabelled taurine was co-administered with radio-labelled taurine, the apparent clearance of taurine was reduced (p<0.0l), suggesting a saturable disposition of taurine from CSF. Distribution of taurine into the choroid plexus, cerebellum, olfactory bulb and cortex was similarly diminished, indicating that the saturable uptake of taurine into these tissues is responsible for the non-linear disposition. A pharmacokinetic model involving first order elimination and saturable distribution described these data adequately. The Michaelis-Menten rate constant estimated from in vivo elimination study is similar to that obtained in the in vitro uptake experiment. Collectively, our results demonstrate that taurine is transported in the choroid plexus via a $Na^+-dependent,saturable$ and apparently ${\beta}-amino$ acid selective mechanism. This process may be functionally relevant to taurine homeostasis in the brain.

• Korean Journal of Plant Tissue Culture
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• v.21 no.3
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• pp.161-166
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• 1994

Since garlics (Allium sativum L.) are propagated through cloves, infection by virus or other pathogens may become severe problem if not using high quality seed bulbs every year resulting in the reduction of yield and bulb quality, In order to solve this problem, the establishment of virus-free bulb production and its supply system have been required because no chemicals were found to eliminate viruses from seed bulbs. This experiment was conducted to develop an effective production technique of high quality seed bulbs using shoot-tip culture. Over 90% of shoot-tips explanted on January L 1990 were survived at constant temperature of either 20, 24 or 28$^{\circ}C$, wheres 88% at alternate temperature (28/20$^{\circ}C$). The growth of shoot and root was most vigorous at constant 24$^{\circ}C$, and least at alternate temperature (28/20$^{\circ}C$) condition. When shoot-tips were explanted June 21 to August 1,1991, survival and growth of shoot-tips was most vigorous on MS medium supplymented with 0.1 mg/L NAA and 2 mg/L kinetin and least 1 mg/L Gh$_3$. The shoot-tips taken from the seed bulbs stored at 4$^{\circ}C$ for 15 to 60 days were placed on MS medium, shoot growth and in vitro bulblet formation increased slightly as affected by the increase of told treatment period at 4$^{\circ}C$.

• Journal of Plant Biotechnology
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• v.29 no.4
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• pp.259-264
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• 2002

Induction of embryogenic callus from Allium wakegi Araki explants was promoted on medium containing 2,4-D, and production of abnormal embryos from the embryogenic callus increased as 2,4-D concentration was raised. Shoot tip was found to be the best explant source for embryogenic callus formation followed in the order by bulb scale and leaf section. Medium containing 0.09 M sucrose was effective for embryogenic callus production. The regenerants from embryogenic callus on medium containing 2,4-D and BA at different concentrations was consisted of diploids, tetraploids and a few mixaploids of 2n＋4n, and their chromosomal aberration rate ranged from 8.0 to 33.3%. Frequency of chromosomal aberrants was the highest (18.7%) in the regenerants obtained from bulb scale-derived embryogenic callus among others. Plant regeneration rate was high (33.5%) from the shoot tip-derived embryogenic callus and the frequency of chromosomal aberrants was very low (7.0%). The plantlets regenerated on medium containing 0.26 M sucrose resulted in high chromosomal aberrants. The regenerants on medium containing sucrose at 0.09∼0.20 M produced chromosomal aberrants at around 15.2∼16.6%.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.31 no.6A
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• pp.417-424
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• 2011

This study focused on development of 60 m span PSC girder considering not only structural performance, but also economical efficiency and constructability including from the improvement of cross-section to the tendon profiles in sequence. Bulb-T type cross section was derived from optimization and actual possibilities to design a bridge were assessed through cross section evaluation. Tendons were also arranged efficiently so that the girder could resist the service load effectively. After developed girder was applied to a sample bridge, result of finite element analysis proved all load steps were satisfied with the allowable stress. Furthermore, it seemed that sufficient redundancy will be available to design a bridge safely. Based on these, a full-scale 60 m span girder was fabricated and 4 point bending test was performed. An initial crack occurred over twice of the service load in this experiment, which establishes adequate structural performance. 60 m span Half-Decked PSC girder developed in this study has a lower height for the given span which resulted from cross section improvement and efficient tendon layout. This girder also has not only the structural advantage, but also advantages in economical efficiency and constructability.

• FLOWER RESEARCH JOURNAL
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• v.17 no.1
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• pp.40-43
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• 2009

'Glory Pink' an interspecific lily cultivar was released in 2007 at National Horticultural Research Institute (NHRI), Rural Development Administration (RDA), Suwon, Korea. The cross and ovule culture was made between FA interspecific hybrid 'FA97-30' (L. formolongi 'Raizan' ${\times}$ Asiatic lily 'A61'), a red colored cultivar, and Asiatic lily 'Sanzio', light red colored cultivar in 2001. Multiplication and bulbing, and characteristic tests were conducted from 2005 to 2006. Flowering time is around the first of July and the plant height is 131.7 cm. Flowers are upward-facing and red-pink (RHS 63C), with size of 11.3 cm. Petal length and width is 9.1 cm and 4.1 cm, respectively. Leaves are 13.8 cm long, 1.4 cm wide. The throat is dark green, and the stigma is light yellow and pollen is light brown. The weight and size of bulb is 51.1 g and 13.2 cm, respectively. For long-term storage, bulbs can be stored under -1 to -2 for year-round forcing.

• Journal of agriculture & life science
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• v.45 no.2
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• pp.29-34
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• 2011

This study was carried out to investigate the flower yield and quality of Lilium Oriental Hybrids as affected by planting date. In cut flower production experiments, effect of planting date and bulb source was studied in rain shelter houses with 30% shading. Four planting dates compared were May 15, June 5, June 25, and July 15. Bulbs used for cut flower production were either imported or produced domestically. The greatest cut flower yield and quality were obtained from bulbs planted either on May 15 or June 25. Bulbs planted on July 15 produced cut flowers of very poor quality and yield, due to high temperatures during the planting time. Therefore, for planting in July, means other than 30% shading have to be adapted to lower temperature during early growth stage. Although plants from imported bulbs had better growth and cut flower yield as compared to domestic bulbs, there was no significant difference.

• Korean Journal of Breeding Science
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• v.43 no.6
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• pp.509-512
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• 2011

A FA intersectional hybrid lily cultivar 'Golden Center' was developed in 2008 at National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) Korea. The cross was conducted between female parent Lilium FA hybrid 'Migreen (FA97-30)' and male parent L. Asiatic hybrid 'Sanzio' by a cut style pollination method (CSM) and immature embryo rescure at Suwon in 1999. The first selection was done and the line name was tentatively given as 'FA04-24' in 2004. After in vitro multiplication, bulb-producing ability, line, growth and flowering characteristic of 'FA04-24' were evaluated from 2005 to 2007. The evaluation of characteristics and consumer preferences were surveyed at a lily flower show of NIHHS in 2008. 'Golden Center' flowers in the middle of June and grows up to 144 cm high in length. Flower blooms facing upward, with light yellow petals (RHS, GW157C). The pollen of 'Golden Center' is sterile. Year-round flowering can be done by storing the bulb under $-1.5^{\circ}C$ conditions. It is needed to control Botrytis disease in wet season.

• Korean Journal of Breeding Science
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• v.49 no.4
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• pp.414-419
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• 2017

Red onion cultivar 'Eumjinara' with mild taste and early maturation was developed in 2013 by the Allium vegetable crop research team, National Institute Crop Science, Rural development Administration (RDA). 'Eumjinara' was originally selected from red onion breeding line, 'IS1115', It was preliminarily selected and designated 'Mon21-31-3' and advanced yield trial was performed from 2008 to 2009. Regional yield trial test was conducted in six regions from 2010 to 2012. Foliage morphology and leaf color were similar to those of a check cultivar, 'Cheonjujeok', but its shape was comparatively broad ovate. Foliage of 'Eumjinara' lodged seven days earlier than check cultivar on May 31 and the average bulb weight ($234{\pm}49.1g/bulb$) was also higher than that of check cultivar. The new cultivar was relatively resistant to clod hardness and downy mildew infection in the field condition. Average yield of 'Eumjinara' in three-year the regional yield trials was 6,999 kg/10a. The total soluble solid contents and quercetin concentrations were $41.43{\mu}g/g$ FW and $556.11{\mu}g/g$ FW, respectively.

• Journal of the Korean GEO-environmental Society
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• v.23 no.9
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• pp.5-16
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• 2022

In recent years due to the development of urban and underground space, the number of ground disasters is increasing, and it is also leading to social problems. To solve the problem, a grouting method is generally used. However, the grouting method has material (grout) limitations in permeability, gelation properties and tensile resistance. Therefore, research on grout materials mixed with fibers is actively carried out to improve the problems. However, in the actual ground injection process, many difficulties have been faced causing the blockage of the inlet port and the injection tube. In this study, 'CNT-mixed grout material' was developed using CNT powder that can reinforce the tensile strength of soils. The uniaxial compressive and tensile strength tests were performed to obtain the optimal content and mechanical properties of the CNT Powder-mixed grout. It was found that the optimal CNT powder content is 0.5% that gives the average maximum strength. A one-dimensional injection test and the bulb formation test were carried out, and it was identified that the injection rate and bulb form could be controlled by pressure and mixing ratio. Field application of the CNT-Mixed grout is simulated using numerical analysis of slopes, foundations, and tunnels reinforced in several types. The positive effect of reducing plastic ranges and settlements was confirmed.

• Korean Journal of Agricultural and Forest Meteorology
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• v.25 no.4
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• pp.276-283
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• 2023

Garlic (Allium sativum L.) is one of the most important vegetables used in various foods in Korea and many countries. The growth of garlic is influenced by various abiotic factors such as cultivation temperature, humidity, minimum temperature duration, and photoperiod. This study investigated the effects of increasing temperatures on the plant growth of the northern- ecotype garlic 'Uiseong' in a temperature gradient tunnel. As a result, temperature increase led to decreases in the bulb diameter, weight, and clove pieces of garlic. The rise of cultivation temperature increased the occurrence rate of incomplete bolting in the Northern-ecotype garlic 'Uiseong', resulting in decreases in productivity and a decrease in the yield of marketable garlic, indicating that temperature increases affect the development of garlic bulb formation. The findings of this study are expected to contribute as foundational data for understanding the growth responses of the northern-ecotype 'Uiseong' to increasing cultivation temperatures. The results of this study can be used to develop designing garlic growth models. In addition, the results of this study can improve understanding the interaction between increased temperature and garlic growth.