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http://dx.doi.org/10.5010/JPB.2002.29.4.259

Chromosome Doubling of Allium wakegi Araki by In Vitro Cultures  

Yim, Sun-Hee (National Plant Quarantine Service, Department of Horiculture, Chonnam National University)
Ahn, Chang-Soon (Department of Horiculture, Chonnam National University)
Jeong, Chang-Nam (National Plant Quarantine Service)
Han, Tae-Ho (National Institute fo Agricultural Biotechnology Rural Development Administration)
Publication Information
Journal of Plant Biotechnology / v.29, no.4, 2002 , pp. 259-264 More about this Journal
Abstract
Induction of embryogenic callus from Allium wakegi Araki explants was promoted on medium containing 2,4-D, and production of abnormal embryos from the embryogenic callus increased as 2,4-D concentration was raised. Shoot tip was found to be the best explant source for embryogenic callus formation followed in the order by bulb scale and leaf section. Medium containing 0.09 M sucrose was effective for embryogenic callus production. The regenerants from embryogenic callus on medium containing 2,4-D and BA at different concentrations was consisted of diploids, tetraploids and a few mixaploids of 2n＋4n, and their chromosomal aberration rate ranged from 8.0 to 33.3%. Frequency of chromosomal aberrants was the highest (18.7%) in the regenerants obtained from bulb scale-derived embryogenic callus among others. Plant regeneration rate was high (33.5%) from the shoot tip-derived embryogenic callus and the frequency of chromosomal aberrants was very low (7.0%). The plantlets regenerated on medium containing 0.26 M sucrose resulted in high chromosomal aberrants. The regenerants on medium containing sucrose at 0.09∼0.20 M produced chromosomal aberrants at around 15.2∼16.6%.
Keywords
Allium wakegi Araki; in vitro chromosome doubling;
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