• Korean Journal of Veterinary Service
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• v.36 no.4
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• pp.321-325
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• 2013

A mass of the adrenal gland was observed during a routine necropsy of a female 23-year-old Asian Water Buffalo (Bubalus bubalis) at Seoul Zoo in Gyeonggi Province, Korea. The animal showed no clinical signs but the necropsy examination revealed hydropericardium, ascites, hydrothorax and edema of the intestinal wall, lung and adrenal gland. Histopathologically, the neoplastic cells of the right adrenal gland were arranged in lobules supported by a fine fibrovascular stroma. The neoplastic cells had round hyperchromatic nuclei and granular eosinophilic to basophilic cytoplasm. Immunohistochemically, tumor cells were positive for chromogranin A and S-100 and negative for vimentin, synaptophysin and cytokeratin. Based on the above findings, this case was diagnosed as a pheochromocytoma. To the best of our knowledge, this is the first report of a pheochromocytoma in an Asian Water Buffalo (Bubalus bubalis).

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.12
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• pp.1666-1673
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• 2011

The presence of sialoglycoproteins (SGPs) in the membranes from goat (Capra aegagrus hircus), buffalo (Bubalus bubalis bubalis) and pig (Sus scrofa domestica) erythrocytes was investigated by partial purification with a chloroform-methanol extraction method followed by Sodium dodecyl sulphate - Polyacrylamide gel electrophoresis in comparison to human (Homo sapiens) erythrocytes. The results show that mammalian erythrocytes possess clear differences in the SGPs numbers and molecular weights although all animals studied in this experiment are from the same class i.e. mammalia. The SGPs number in human, goat, buffalo and pig are four (PAS-1 to PAS-4), ten (PAS-GI to PAS-GX), seven (PAS-BI to PAS-BVII) and four (PAS-PI to PAS-IV) respectively as indicated by staining the polyacrylamide gel with sialoglycoprotein-specific Periodic acid-Schiff's (PAS) stain. The new SGPs could be observed only after the partial purification of membrane fractions named as PAS-HI with molecular weight (Mr) 190 kDa and PAS-HII 150 kDa in human, PAS-BIA in buffalo and PAS-PIA and PAS-PIVA in pig. The gels were also stained with Coomassie brilliant blue (CBB) and Silver stain to check the contamination of other membrane proteins in the purified fractions. The quantitative distribution of SGPs was also determined by densitometry. Present study indicates that there are some basic differences in mammalian erythrocyte membrane SGPs, especially with respect to their number and molecular weights indicating major structural variations.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.5
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• pp.617-621
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• 2006

Recently the numbers of the Thai swamp buffalo (Bubalus bubalis), a native species of Thailand, have been rapidly declining, leading to a requirement for conservation programs for this breed. Such studies of the genetic diversity of this species are essential for conservation decisions and to assist the rational implementation of breeding programs. In this study, the genetic diversity of 80 Thai swamp buffalo, randomly selected from seven different research stations of the Thai Department of Livestock Development, were studied using ten cattle microsatellite markers. Polymorphic PCR products were observed at all microsatellite loci, with percentages of polymorphic loci ranging from 80.00 to 100.00%. The population from Payao showed the lowest level of polymorphism. The mean number of alleles per locus was 4.7 with the highest number of alleles being eight (ETH152) and the lowest being three (HAUT27 and ILSTS030). The average unbiased heterozygosity for all seven populations was 0.61 and varied between 0.5314 (Samui) and 0.6798 (Surin). The genetic distance according to NEI's (1972) ranged from 0.0722 to 0.4427. The populations from Surin and Burirum are the closest populations, while populations from Samui and Payao are the most divergent. The information generated by this study will greatly aid in the establishment of effective breeding and conservation programs for the Thai swamp buffalo.

• Asian-Australasian Journal of Animal Sciences
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• v.2 no.3
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• pp.524-526
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• 1989

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.3
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• pp.321-325
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• 2002

Karyotype analysis was carried out on blood samples of 30 water buffaloes belonging to different breed groups (i.e. Philippine Carabao (PC), Indian Murrah (IM), Bulgarian Murrah (BM), "$F_1$ 50% IM-50% PC", "$F_1$ 50% BM-50% PC" and "75% IM-25% PC"), using the modified Leucocyte Culture Technique. The modal chromosome numbers of the PC, "$F_1$ 50% IM-50% PC", "$F_1$ 50% BM-50% PC", IM, BM and "75% IM-25% PC" were 2n=48, 49, 49, 50, 50 and 50, respectively. The water buffalo chromosomes are mostly acrocentric (79.67%) and the remainder submetacentric (20.33%). Results of the ordinary least square analysis showed significant breed effects (p<0.01) on other karyotypic characteristics (i.e. relative length, arm ratio and centromeric index). Significant correlation between karyotypic characteristics and some animal performance traits were also found. The significant correlation values imply that karyotypic characteristics can be used as important criteria to select potentially productive young water buffaloes. In the future, more production and reproduction traits from non-institutional herds should be included in the analysis to reveal meaningful correlations with various karyotypic characteristics.

• Asian-Australasian Journal of Animal Sciences
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• v.2 no.3
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• pp.228-230
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• 1989

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.3
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• pp.393-398
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• 2007

The present study was carried out to investigate the serum minerals profile in buffaloes (Bubalus bubalis) suffering from parturient haemoglobinuria (PHU) along with minerals profile of soils and fodders from the disease prone areas and their interrelationships. Serum samples were collected from 60 each of healthy and PHU affected buffaloes randomly selected from field cases. Serum samples were collected from each animal. Fifty composite soil samples were collected where PHU was prevalent. Fifty samples of fodders including leaves and stems being fed to the diseased buffaloes were collected. The difference in the levels of calcium and potassium between upper and lower soil surface of disease prone areas under study were statistically non-significant. The mean values of phosphorous, copper, iron, selenium and molybdenum in upper soil surface were significantly (p<0.05) higher than in lower soil surface. None of the fodders offered to the diseased animals met the dietary requirements of phosphorus and copper whereas none of the fodders was deficient in potassium, iron and selenium rather were having excess of potassium, iron and selenium. The concentration of calcium was adequate in lucerne, berseem, sarson and sorghum, while maize, sugarcane and wheat straw did not meet the required levels for dairy animals. Molybdenum contents in all fodders were adequate to meet the dietary requirements of the dairy buffaloes. Serum phosphorus, copper and selenium were significantly (p<0.001) lower whereas potassium, iron and molybdenum in buffaloes suffering from PHU were significantly (p<0.001) higher than in healthy buffaloes. It was concluded that phosphorous deficient soils play a major role by transferring this deficiency to plants and ultimately reaching to animals where hypophosphataemia is a consistent finding.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.7
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• pp.1046-1048
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• 2003

A polymerase chain reaction (PCR) assay was developed to differentiate buffalo meat from the meat of Ceylon spotted deer (Axis axis ceylonensis), Ceylon sambhur (Cervus unicolor unicolor), cattle (Bovine), goat (Caprine), pig (Porcine), and sheep (Ovine). A set of primers were designed according to the sequence of the mitochondrial cytochrome b gene of bubalus bubalis and by PCR amplification a band of approximately 242 bp band was observed with buffalo DNA. These primers did not cross-react with DNA of other animal species tested in the study under the specified reaction conditions. A band of 649 bp was observed for all animal species tested when DNA was amplified with the universal primers indicating the presence of mitochondrial DNA in the samples. The technique was sensitive enough to identify rotten (10 days post slaughter), dried and cooked buffalo meat. The absence of a cross reaction with human DNA using the buffalo specific primers eliminates possible false positive reactions.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.1
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• pp.124-131
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• 2007

There is worldwide interest in buffalo as an animal for meeting the growing demands of meat, milk and work in the developing countries. One of the major constraints to full exploitation of the productive potential of buffalo has been its inherently low reproductive efficiency as reflected by late maturity, poor expression of oestrus, silent oestrus, irregular oestrous cyclicity, seasonality in breeding, anoestrus, low conception rate, long postpartum interval, repeat breeding etc. Ovarian cyclicity is regulated by endocrine and neuroendocrine mechanisms namely hypothalamic hormones, gonadotropins and ovarian steroids. Detailed endocrine investigations are suggested with special reference to the hypothalamo-hypophysial-ovarian axis to gain a better understanding of reproduction in buffalo and to modify it to derive the maximum benefit from this animal.

• Asian-Australasian Journal of Animal Sciences
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• v.4 no.1
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• pp.1-5
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• 1991

Responsiveness of the testis to pregnant mare's serum gonadotropin (PMSG) was studied in immature Nili-Ravi buffalo bulls. Four month old bull calves weighing between 66 to 100 kg raised under uniform condition, were treated with 1000 IU PMSG or vehicle, daily, for six days. PMSG induced an increase in the size of the testis, enlargement of the seminiferous tubules and activation of the spermatogonia. The number of differentiated Leydig cells in the testis of gonadotropin treated animals increased considerably over that of the control testes. A significant increase in plasma testosterone concentrations was observed 24 h following the first injection of PMSG and the levels continued to increase until day 6. In vehicle treated animals plasma testosterone levels remained more or less at pretreatment values. The data suggest that buffalo bull testis is functionally responsive to gonadotropin at an early stage of prepubertal development.