• Korean Journal of Veterinary Service
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• v.32 no.4
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• pp.347-351
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• 2009

Bruella spp. involves a considerable danger of public health and farm animal industry. In this study, we assessed the disinfection efficacy of alkaline disinfectant solution and three commercial farm disinfectants (quaternary ammonium compound, sodium dichloroisocyanurate, potassium monopersulphate/sodium dichloroisocyanurate) against Brucella ovis. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of selected disinfectants following exposure to test bacteria for 30 minutes at $4^{\circ}C$. Disinfectants and test bacteria are diluted with distilled water (DW), hard water (HW) or organic matter suspension (OM) according to treatment condition. Three commercial disinfectant showed excellent antimicrobial activity (up to dilution of $\times200$ in OM treatment). Alkaline disinfectant solution demonstrated favorable bactericidal efficacy against B. abortus (at dilution of $\times20$ in OM treatment). Three commercial farm disinfectants possess excellent efficacy against B. ovis. Alkaline disinfectant solution has lower potency than commercial farm disinfectant but could help to limit the spread of brucellosis.

• Korean Journal of Veterinary Research
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• v.40 no.3
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• pp.543-549
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• 2000

The pathogenicity of Br abortus RB51 strains, producted by commercial vaccine companies in Republic of Korea and USA, were evaluated in mouse and guinea pig. BALB/c and ICR mice were intraperitoneally inoculated with RB51 vaccines or virulent field strain and the existence of RB51 including its ratio of spleen to weights and persistence in spleens were examined. Groups of guinea pigs on day 55-58 of received subcutaneously with various RB51 vaccines, RB51 field isolates (Daehungjin) or virulent field isolates(Sangju) to compare the histopathogenicity in uterus. All the mice received RB51 vaccines or RB51 field isolates survived for 10 days, but the groups of mice received virulent field isolates died 5 from 11 (45.5%) in case of BALB/c mice and 12 from 12 (100%) in ICR mice, respectively. The number of RB51 in the groups of mice given with vaccine strains and RB51 field isolates were declined rapidly were in spleens between 12 and 20 days after inoculation. In contrast the mice given with the virulent field isolates rose in number of bacteria up to 20 days after inoculation. In the groups of mice infected with virulent field isolates, the ratio of spleen weights to body weight were significantly higher than those in control or in the groups inoculated with RB51 strain, including RB51 field isolates, at 12 and 20 days after inoculation. At ten days after inoculation, placentas of both the pregnant and non-pregnant guinea pigs were conducted for histopathological examination. Although any abnormal lesions were not observed in non-pregnant guinea pigs, all the strains caused the inflammation of the placenta, implying pathogenecity of RB51 in pregnant guinea pigs.

• Korean Journal of Veterinary Research
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• v.48 no.4
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• pp.431-440
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• 2008

Brucellosis is one of the most important zoonosis in worldwide. As one of the control measures, attempts have been made to develop new diagnostic methods using filed isolates as a national policy in many countries. Currently, bovine brucellosis in Korea have been received attention in both public health and economical aspects due to sudden increase of outbreak. Based on the situation, we compared standard strain (B. abortus 1119-3) with field isolates to reveal the differences among them. Biological and biochemical charateristics, antibiotic resistance profiles, outer membrane proteins (OMPs) and lipopolysaccharide analysis of the strains were included in this study. For the diagnostic purpose, an attempt was made to find out a novel antigen from the Korean isolates by serological analysis. There were differences about 55 kDa, 36-38 kDa and 20 kDa in analysis of OMPs by SDS-PAGE and Western blot with positive sera ($\geq$ 1:400 in SAT titer). Also, a serological diagnostic method, ELISA was conducted using OMPs of the strains as novel antigen. Relationships between O.D. and SAT titer were analyzed using field sera showing different SAT titer. High correlation coefficient was observed between SAT titer and ELISA. Results from this study suggested that a new diagnsotic method should be developed using their own field isolates in each country.

• Journal of Veterinary Science
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• v.22 no.2
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• pp.15.1-15.13
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• 2021

Background: Attenuated Salmonella strain can be used as a vector to transport immunogens to the host antigen-binding sites. Objectives: The study aimed to determine the protective efficacy of attenuated Salmonella strain expressing highly conserved Brucella immunogens in goats. Methods: Goats were vaccinated with Salmonella vector expressing individually lipoprotein outer-membrane protein 19 (Omp19), Brucella lumazine synthase (BLS), proline racemase subunit A (PrpA), Cu/Zn superoxide dismutase (SOD) at 5 × 10⁹ CFU/mL and challenge of all groups was done at 6 weeks after vaccination. Results: Among these vaccines inoculated at 5 × 10⁹ CFU/mL in 1 mL, Omp19 or SOD showed significantly higher serum immunoglobulin G titers at (2, 4, and 6) weeks post-vaccination, compared to the vector control. Interferon-γ production in response to individual antigens was significantly higher in SOD, Omp19, PrpA, and BLS individual groups, compared to that in the vector control (all p < 0.05). Brucella colonization rate at 8 weeks post-challenge showed that most vaccine-treated groups exhibited significantly increased protection by demonstrating reduced numbers of Brucella in tissues collected from vaccinated groups. Real-time polymerase chain reaction revealed that Brucella antigen expression levels were reduced in the spleen, kidney, and parotid lymph node of vaccinated goats, compared to the non-vaccinated goats. Besides, treatment with vaccine expressing individual antigens ameliorated brucellosis-related histopathological lesions. Conclusions: These results delineated that BLS, Omp19, PrpA, and SOD proteins achieved a definite level of protection, indicating that Salmonella Typhimurium successfully delivered Brucella antigens, and that individual vaccines could differentially elicit an antigen-specific immune response.

• The Journal of the Korean Society for Microbiology
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• v.35 no.5_6
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• pp.377-377
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• 2000

• Journal of the korean veterinary medical association
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• v.43 no.9
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• pp.817-824
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• 2007

브루셀라증(brucellosis)은 세포내 기생하는 브루셀라균에 의해 발생하는 인수공통감염질환으로써, 결핵과 유사하게 만성육아종성 염증을 일으키고 장기간의 병합 항균요법을 필요로 하는 질환이다. 그럼 음성 단간균(coccobacilli)인 브루셀라균은 bartonella 및 리켓챠 등과 함께 proteobacteria의 2종에 속하며 전통적으로는 주로 침범하는 숙주에 따라 6종으로 분류되는데, 그 중 Brucella melitensis(양, 염소), B. abortus (소), B. suis (돼지) 및 B. canis (개) 등의 4종이 사람 브루셀라증을 일으키는 것으로 알려져 있다.

• Korean Journal of Veterinary Research
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• v.45 no.1
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• pp.45-53
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• 2005

GroE that is a heat shock protein composed of GroEL and GroES is known as an immunodominant target of both the humoral and cellular immune responses in bovine brucellosis. This study was carried out to characterize groE gene encoding heat shock proteins of B. abortus isolated in Korea and to evaluate the immunogenicity of the GroE protein expressed in E. coli system. In PCR the specific signals with the size of 2,077 bp were detected in five strains isolated from the mammary lymphnodes of the dairy cattle that were serologically positive and the reference strains. In comparison of the sequences of nucleotides and amino acids among the strains, GroES showed 100% identity in both sequences. GroEL was evaluated 99.0~99.9% in nucleotides and 98.0~100% homology in amino acids. The groE gene including groES and groEL was inserted into pET29a vector and constructed pET29a-GroE recombinant plasmids. The inserted groE was confirmed by digestion with Nco1 and EcoR1 endonucleases and nucleotide sequencing. E. coli BL (DE3) was transformed with pET29a-GroE, named as E. coli BL (DE3)/pET29a-GroE. In SDS-PAGE, it was evident that the recombinant plasmid effectively expressed the polypeptides for GroES (10 kDa) and GroEL (60 kDa) in 0.5, 1 and 2 hours after IPTG induction. The immuno-reactivity of the expressed proteins were proved in mouse inoculation and Western blot analysis.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.8
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• pp.1089-1095
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• 2010

Four biological candidate genes, natural resistance associated macrophage protein 1 (SLC11A1 or NRAMP), prosaposin (PSAP), interferon Gamma (IFNG), and toll-like receptor 4 (TLR4), were examined to identify single nucleotide polymorphisms (SNP) and associations of the SNP with antibody response kinetics in hens. An $F_2$ population was produced by mating $G_0$ highly inbred (<99%) males of two MHC-congenic Fayoumi lines with highly inbred Leghorn hens. The $F_2$ hens (n = 158) were injected twice with SRBC and whole, fixed Brucella abortus (BA). Blood samples were obtained before each immunization, at 7 d after primary immunization, and at several time points after secondary immunization. Minimum titers (Ymin) and the time needed to reach them (Tmin), and maximum (Ymax) titers and the time needed to reach them (Tmax), were estimated from the seven post-secondary immunization titers using a nonlinear regression model. The $F_2$ hens were genotyped for the four candidate genes by using PCR-RFLP for one SNP per gene, which identified the parental allele. General linear models were used to test associations of SNP genotypes with antibody response parameters and BW measured at 4 ages. The IFNG SNP was highly significantly (p<0.0125) associated with primary response to SRBC, Tmin to BA, Ymin to BA, and 12-week BW. The current study demonstrated that the novel IFNG promoter SNP was associated with antibody kinetics for BA and SRBC in laying hens, and also with BW, suggesting that this cytokine may play a pivotal role in the relationship between immune function and growth.

• Korean Journal of Veterinary Service
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• v.32 no.3
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• pp.219-225
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• 2009

A cross sectional survey was conducted to determine the seroprevalence of brucellosis in cattle in Bangladesh Agricultural University (BAU) Veterinary Clinics, in BAU Dairy Farm and Vabokhali from June 2008 to November 2008. A total of 200 serum samples were collected from BAU Veterinary Clinic, from BAU Dairy Farm and Vabokhali. Among the serum samples 143 sera samples were collected from BAU Veterinary Clinic, 42 serum samples from BAU Dairy Farm and 15 serum samples from Vabokhali. Sera were separated from blood samples and tested with specific Brucella abortus antigen (BAA) test and B. melitensis antigen (BMA) test. The overall seroprevalence of brucellosis in cattle was 5% in BAA and 0.5% in BMA. It was observed that, a significant higher prevalence of B. abortus was found in female than male. An insignificant higher prevalence of brucellosis was found in adult cattle (aged above 5 years), in cross breed cattle, in cattle with grazing, cattle breed by natural breeding, and in pregnant cows. Although insignificant but a higher prevalence of brucellosis was found in aged cattle than young cattle, cross bred cattle, pregnant cattle than non pregnant cattle, cattle with grazing. A higher prevalence of brucellosis was found in female cattle than male.

• Korean Journal of Veterinary Service
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• v.32 no.4
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• pp.335-341
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• 2009

Canine brucellosis produce abortions and infertility in dogs and is currently diagnosed by serological methods such as rapid slide agglutination test with 2-mercaptoethanol (2-ME RSAT) and immunochromatographic assay (ICA). Bacterial isolation is considered gold standard for Brucella diagnosis and the polymerase chain reaction (PCR) is an alternative method to bacterial isolation. A total of 36 whole blood samples were collected from dogs reared in area of Chuncheon and were subjected to serology (2-ME RSAT and ICA for B. canis, Rose Bengal test and C-ELISA for B. abortus), blood culture and 3 types of PCRs (BSCP31, 16s rRNA, and OMP-2). All blood samples were negative by serology and blood cultures. The BCSP31 and the OMP-2 PCR detected 5 samples were positive whereas the 16S rRNA PCR detected all samples were negative as serological methods and blood culture did. From the results observed in the present study, we conclude that 16S rRNA PCR could be used for direct PCR for canine blood samples.