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Effects of Lowering the Dietary Levels of Energy, Protein and Amino Acid (Methionine and Cysteine) on the Performance of Laying Hens

  • Serpunja, Subin;Balasubramanian, Balamuralikrishnan;Kim, In Ho
    • Korean Journal of Poultry Science
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    • v.44 no.2
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    • pp.67-73
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    • 2017
  • The purpose of our study was to determine the effects of varying levels of energy, protein, and amino acids on the performances of laying hens. A total of 240 Hy-Line Brown laying hens at 36 weeks of age were used in this 4-week feeding trial. The hens were randomly allocated to five treatment diets, with eight replications of six hens in each replicate cage. The treatment diets were as follows: A- basal diet + 18% crude protein, metabolizable energy 2,800 kcal, total (methionine + cysteine) 0.65%; B- basal diet + 17% crude protein, metabolizable energy 2,700 kcal, total (methionine + cysteine) 0.59%; C- basal diet + 16.5% crude protein, metabolizable energy 2,700 kcal, total (methionine + cysteine) 0.59%; D- basal diet + 16.5% crude protein, metabolizable energy 2,700 kcal, total (methionine + cysteine) 0.54%; and E- basal diet + 16% crude protein, metabolizable energy 2,680 kcal, total (methionine + cysteine) 0.54%. The study results revealed that the hen-day egg production of hens that were fed with low-energy diets (B, C, and D) was comparable with that of hens fed with high-energy diet A, whereas average daily feed intake in hens fed treatment diet D and E was significantly higher (P<0.05) than that in hens fed treatment diet A. Overall, the eggshell thickness was unaffected by any of the treatment diets. Egg weight was comparable among the treatment diets, except for treatment diet E. Haugh unit improved with decreasing levels of dietary energy, protein, and methionine + cysteine in the diet. We can summarize that laying hens fed with low dietary energy and low crude protein treatment diets B, C, and D had satisfactory performance compared with those fed with high-energy treatment diet A. This indicates that there is the potential to reduce feed costs by formulating diets with lower energy and low protein levels.

Occurrence of Stem Rot of Houttuynia cordata Thunb. Caused by Sclerotium rolfsii (Sclerotium rolfsii에 의한 약모밀 흰비단병)

  • Park Kyeng-Seok;Park Jun-Hong;Kim San-Young;Choi Jang-Soo
    • Research in Plant Disease
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    • v.12 no.1
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    • pp.55-57
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    • 2006
  • Stem rot of Houttuynia cordata occurred in the experiment field of Research Institute of Natural Product, Uiseong, Gyeongbuk, Korea in July 2003. The typical symptoms of the disease were water-soaking, stem rot, necrotic tuber. White mycelial mats were spread over lesions and sclerotia were formed on died plant and near soil line. The sclerotia were spherical and irregular, 1-2.5 mm on potato dextrose agar (PDA), white to brown in color, The optimal temperature for hyphal growth was $28{\sim}30^{\circ}C$ and the hyphal diameter was $3{\sim}9{\mu}m$. Clamp connections were observed on PDA. Pathogenicity of the casual organism was provided on H. cordata according to Koch's postulate. The fungus was identified as Sclerotium rolfsii Saccardo on the basis of mycological characteristics and pathogenicity test to host plants. This is the first report on the stem rot of H. cordata caused by S. rolfsii in Korea.

Development of Meta Problem Types to Improve Problem-solving Power (문제 해결력 신장을 위한 베타 문제 유형 개발)

  • 현종익
    • Education of Primary School Mathematics
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    • v.2 no.1
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    • pp.3-13
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    • 1998
  • In mathematics education we have focused on how to improve the problem-solving ability, which makes its way to the new direction with the introduction of meta-cognition. As meta-cognition is based on cognitive activity of learners and concerned about internal properties, we may find a more effective way to generate learners problem-solving power. Its means that learners can regulate cognitive process according to their gorls of learning by themselves. Moreover, they are expected to make active participation through this process. If specific meta problems designed to develop meta-cognition are offered, learners are able to work alone by means of their own cognition and regulation while solving problems. They can transfer meta-cognition to the other subjects as well as mathematics. The studies on meta-cognition conducted so far may be divided into these three types. First in Flavell([3]) meta-cognition is defined as the matter of being conscious of one's own cognition, that is, recognizing cognition. He conducted an experiment with presschoolers and children who just entered primary school and concluded that their cognition may be described as general stage that can not link to specific situation in line with Piaget. Second, Brown([1], [2]) and others argued that meta-cognition means control and regulation of one's own cognition and tried to apply such concept to classrooms. He tried to fined out the strategies used by intelligent students and teach such types of activity to other students. Third, Merleary-Ponty (1962) claimed that meta-cognition is children's way of understanding phenomena or objects. They worked on what would come out in children's cognition responding to their surrounding world. In this paper following the model of meta-cognition produced by Lester ([7]) based on such ideas, we develop types of meta-cognition. In the process of meta-cognition, the meta-cognition working for it is to be intentionally developed and to help unskilled students conduct meta-cognition. When meta-cognition is disciplined through meta problems, their problem-solving power will provide more refined methods for the given problems through autonomous meta-cognitive activity without any further meta problems.

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Block Unit to Present Panoramic View in 3D Map (3차원 지도에서 파노라마 전경 표현을 위한 단위 블록 디자인에 관한 연구)

  • Kim, Sung-Kon
    • Science of Emotion and Sensibility
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    • v.12 no.1
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    • pp.137-144
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    • 2009
  • This study aims to develop a 3D map which allows users to better understand the overall topography and to memorize the general concepts of the current location for tourists. The Map also utilizes different forms and color codes of the block units in the panoramic view. First, in preparation for making the road block unit, 28 types of roads presented in common map were defined. Based on the definitions, 12 dominant groups were formed. In designing each form, visual correlation was considered. Second, 42 types of terrain features presented in normal maps were defined for making the panoramic block unit. Then, 2 dominant groups, each contains 5 subgroups, were made according to the assemble pattern and the size of area. On the basis of the grouped units, the panoramic block units were produced with 3D pole line graphics maintaining integration. Lastly, each panoramic block units were categorized by color classes such as blue, green, gray and brown class. The overall color combination of the work was evaluated as well harmonized since the Moon&Spencer's Aesthetic measure value exceeds 0.5.

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Determination of Energy and Nutrient Utilization of Enzyme-treated Rump Round Meat and Lotus Root Designed for Senior People with Young and Age d Hens as an Animal Model

  • Park, Okrim;Kim, Jong Woong;Lee, Hong-Jin;Kil, Dong Yong;Auh, Joong-Hyuck
    • Food Science of Animal Resources
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    • v.36 no.1
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    • pp.109-113
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    • 2016
  • This study aimed to examine the nutrient utilization of rump round meat and lotus root using young (32 wk) and aged hens (108 wk) as an animal model. Rump round meat and lotus root were prepared with or without enzymatic treatment. For each age group of laying hens, a total of 24 Hy-Line Brown laying hens were randomly allotted to one of two dietary treatments with six replicates. For rump round meat, the true total tract retention rate (TTTR) of dry matter (DM) and nitrogen (N) were unaffected by either enzymatic treatment or hen age. However, aged hens had greater (p<0.01) TTTR of energy and crude fat than young hens. Enzymatic treatment did not influence the TTTR of energy or crude fat. In addition, we did not observe any significant interaction between the TTTR of DM, energy, N, or crude fat in rump round meat and hen age or enzymatic treatment. The TTTR of DM remained unchanged between controls and enzyme-treated lotus root for young hens. However, enzyme-treated lotus root exhibited greater (p<0.05) TTTR of DM than control lotus root for aged hens, resulting in a significant interaction (p<0.05). The TTTR of energy and N in lotus roots were greater (p<0.01) for aged hens than for young hens. In conclusion, enzymatic treatment exerted beneficial effects on energy and nutrient utilization in aged hens, suggesting the aged hen model is practical for simulation of metabolism of elderly individuals.

Isolation of Eckol from Ecklonia cava via Centrifugal Partition Chromatography (CPC) and Characterization of it's Anti-inflammatory Activity (고속원심분배 크로마토그래피를 이용한 감태(Ecklonia cava)로부터 Eckol의 분리 및 항염증 활성)

  • Kim, Yoon Taek;Lee, Ji-Hyeok;Ko, Ju-Young;Oh, Jae-Young;Lee, Won-U;Sok, Chang Hyun;Hong, Jin Tae;Jeon, You-Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.48 no.3
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    • pp.301-307
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    • 2015
  • Phlorotannins and marine algal polyphenols, including dieckol, 6,6-bieckol, phloroglucinol, phlorofucofuroeckol-A, and eckol, were isolated from brown seaweeds. These compounds have beneficial bioactivities, and Ecklonia cava has become widely used for the extraction and isolation of phlorotannins. Eckol, in particular, has been to shown to have antioxidant, anti-inflammatory, anticoagulatory, and photoprotective properties. However, due to its low abundance in weaweed, the isolation and purification of eckol are difficult. Its limited availability renders the isolation and purification of eckol labor-intensive processes. Centrifugal partition chromatography (CPC) is an efficient technique for the isolation and purification of eckol. In this study, eckol was isolated from the ethyl acetate fraction of the 70% ethanol extract of E. cava using CPC with a two-phase solvent system of a n-hexane:EtOAc:methanol:water (2:8:3:7, v/v) solution. The purity and anti-inflammatory activity of the isolated eckol were verified by high-performance liquid chromatography and by assaying lipopolysaccharide-induced inflammatory responses in an immortalized murine BV2 microglial cell line, respectively. In conclusion, CPC is a useful technique for simple and efficient isolation of eckol from E. cava.

Effect of superdosing phytase on productive performance and egg quality in laying hens

  • Kim, Jong Hyuk;Pitargue, Franco Martinez;Jung, Hyunjung;Han, Gi Ppeum;Choi, Hyeon Seok;Kil, Dong Yong
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.7
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    • pp.994-998
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    • 2017
  • Objective: An experiment was conducted to determine the effect of superdosing phytase on productive performance and egg quality in laying hens. Methods: A total of 200 42-wk-old Hy-Line Brown laying hens were allotted into 1 of 5 dietary treatments with 5 replicates consisting of 8 hens per replicate. The positive control (PC) and negative control diets (NC) were prepared based on the recommended P levels in layer diets. Supplemental phytase was added to the negative control diet at 10,000 (SD10), 20,000 (SD20), or 30,000 (SD30) fytase units (FTU)/kg. Productive performance was summarized for 6 weeks from 42 weeks to 47 weeks of age. Egg quality was assessed from 4 eggs per replicate randomly collected at the conclusion of the experiment. Results: The SD20 treatment had greater (p<0.05) hen-day egg production than PC, NC, and SD10 treatment groups. There was no difference in hen-day egg production between SD20 and SD30 treatment groups. However, SD30 treatment had greater (p<0.05) hen-day egg production than PC treatment, but showed no difference in hen-day egg production as compared to NC and SD10 treatment groups. However, egg weight, egg mass, feed intake, and feed conversion ratio were not affected by dietary treatments. Egg quality including eggshell strength, eggshell color, egg yolk color, and haugh unit was not influenced by dietary treatments. Conclusion: Superdosing level of 20,000 FTU/kg phytase in diets has a positive effect on egg production rate, but no beneficial effect on egg quality in laying hens.

Stem Rot of Perilla Caused by Sclerotium rolfsii in Korea (Sclerotium rolfsii에 의한 들깨 흰비단병)

  • Kwon, Jin-Hyeuk;Choi, Si-Lim;Lee, Heung-Su;Shim, Hong-Sik
    • The Korean Journal of Mycology
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    • v.40 no.3
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    • pp.177-178
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    • 2012
  • Stem rot of perilla (Perilla frutescens var. japonica Hara) occurred sporadically in the exhibition field at Gyeongsangnam-do Agricultural Research and Extension Services in Korea. The infected stems are water-soaked, rotted, wilted and finally leads to the death of the whole plants. The sclerotia, 1-3 mm, white to brown, spherical, were formed on lesions and the surface of soil line. The optimum temperature for mycelial growth and sclerotia formation was $30^{\circ}C$ on potato dextrose agar and the hyphal width was 4-9 ${\mu}m$. The typical clamp connections were observed in the hyphae of the fungus. On the basis of mycological characteristics and pathogenicity to host plants, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report of stem rot on perilla caused by S. rolfsii in Korea.

Stem Rot of Convallaria keiskei Caused by Sclerotium rolfsii (Sclerotium rolfsii에 의한 은방울꽃 흰비단병)

  • Kwon, Jin-Hyeuk;Lee, Heung-Soo;Kang, Dong-Wan;Kwack, Yong-Bum
    • The Korean Journal of Mycology
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    • v.39 no.2
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    • pp.145-147
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    • 2011
  • Stem rot symptoms of Convallaria keiskei occurred sporadically in the herb exhibition field at Hamyang-gun Gyeongnam province in Korea. The typical symptom is water-soaking on the main stem, rotting, wilting, and blighting, which eventually leads to death of the plant. The sclerotia, which were white to brown in color, globoid in shape with size of 1~3 mm hyphal width of which was 4~9 ${\mu}m$, were formed over lesions and surface soil line. The optimum temperature for mycelial growth and sclerotia formation was $30^{\circ}C$ on PDA. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. Mycological characteristics and pathogenicity to host plants indicated that the infected fungus was Sclerotium rolfsii Saccardo. This is the first report of stem rot on Convallaria keiskei caused by S. rolfsii in Korea.

Growth and Maturation of Laminaria japonica Transplanted for Seaforest Construction on Barren Ground (갯녹음 해역에 해중림 조성을 한 다시마의 생장과 성숙)

  • Kim, Young-Dae;Hong, Jung-Pyo;Song, Hong-In;Jeon, Chang-Yeong;Kim, Su-Kyoung;Son, Yong-Soo;Han, Hyoung-Kyun;Kim, Dong-Sam;Kim, Jin-Hee;Kim, Myoung-Rae;Gong, Yong-Gun;Kim, Dae-Kweon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.40 no.5
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    • pp.323-331
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    • 2007
  • Barren ground is an abnormal phenomenon in coastal ecosystems where seaweeds are destroyed and largely replaced by coralline algae containing calcium carbonate components. To restore the seaweed forest, we attempted reconstruction in an area of Samcheok, Korea, where barrenness is extensive. We developed two methods of seaforest construction underwater longline and seed transplantation for the brown seaweed Laminaria japonica, a fast-growing edible seaweed. The blade length of L. japonica attached to an underwater long line was $93.9{\pm}38.2cm$ and blade weight $7.6{\pm}2.1g$ in February 2004. Seaweed size declined after August 2004. A Similar pattern was observed using the transplantation method with maxima of $179.3{\pm}40.3cm\;and\;14.9{\pm}3.2g$ for blade length and weight, respectively in July 2004. The transplanted seaweeds matured in October as did a wild population. These results indicate that transplanted seaweed acclimate to new environments.