• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.6
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• pp.803-806
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• 1993

The properties of proteolytic enzymes from the fruit of Broussonetia Kazinoki Siebold were investigated. The protease activity of the enzymes from the fruit of Broussonetia Kazinoki Siebold was 1.6 unit. The optimum temperature and pH of the enzymes were $60^{\circ}C$ and 7.0, respectively. The enzymes were stable at pH values from 6 to 8 for 1 hr. at $37^{\circ}C$ of incubation and also retained all activity after incubation for 1 hr. at $60^{\circ}C$. The enzyme preparations showed strong activities toward hemoglobin and collagen.

• 한국약용작물학회:학술대회논문집
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• 2012.05a
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• pp.139-140
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• 2012

• Proceedings of the Korean Society of Life Science Conference
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• 2007.10a
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• pp.124.1-124.1
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• 2007

See Full Text

• 한국약용작물학회:학술대회논문집
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• 2012.05a
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• pp.141-142
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• 2012

• 한국약용작물학회:학술대회논문집
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• 2012.05a
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• pp.143-144
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• 2012

• Korean Journal of Plant Resources
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• v.32 no.6
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• pp.714-722
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• 2019

Oxidative DNA damage negatively affects humans and the research is currently ongoing to find ways to reduce oxidative stress. Oxidative stress has been identified as a key factor in triggering various diseases. Thus, its alleviation is important for human health. Broussonetia kazinoki (B. kazinoki) has been used in traditional Korean medicine as a dermatological therapy to treat burns, pruritus, and acne. B. kazinoki is generally segregated into peeled root (PR), root bark (RB), peeled stem (PS), and stem bark (SB). To assess these components for their antioxidant activity and protection against DNA damage, their ethyl acetate fractions were examined by 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay. As a result of confirming the expression of factors involved in attenuating DNA damage, the protective effect of SB on oxidative stress suppressed the expression of p-p53 and γ-H2AX. Additionally, the levels of p53 and H2AX mRNA were significantly downregulated. In conclusion, these results indicated that the SB component of B. kazinoki had the potential to be used as an effective natural antioxidant compared to the other parts of the plant.

• Korean Journal of Plant Resources
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• v.36 no.4
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• pp.290-298
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• 2023

Several members of the genus Broussonetia are woody plants with high-quality cellulose fibers and are used to make a traditional type of Korean paper known as Hanji. Three of these species, Broussonetia kazinoki, Broussonetia monoica, and Broussonetia papyrifera, are found in the Korean Peninsula. Because it is challenging to distinguish different Broussonetia species based on morphology alone, we have developed a set of insertion/deletion (InDel) markers for genetic identification of these species. From twenty-two Broussonetia samples collected throughout Korea, we selected six for next-generation sequencing analysis. InDel marker candidates were identified by comparing this sequence information with the B. kazinoki chloroplast genome sequence. The marker candidates were used to screen the genomes of the twenty-two Broussonetia plants, and five useful chloroplast-based InDel markers were identified. Detailed genotyping using these five markers showed that the twenty-two plants of the genus Broussonetia could be clustered into five groups, verifying that the markers developed here can be used for breeding, identification, and analysis of species in the genus Broussonetia.

• Journal of the Korean Wood Science and Technology
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• v.42 no.3
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• pp.223-230
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• 2014

This study examined emission characteristics of far-infrared ray, formaldehyde, volatile organic compounds and deodorization rate of particleboard prepared at different target board densities and resin content levels, using the xylem part of Broussonetia Kazinoki Sieb. The deodorization rate increased, as the target density of board and resin content increased. Emission rates of the far-infrared ray and total volatile organic compounds (TVOCs) were 0.892~0.899, and $0.074mg/m^2{\cdot}h$ for particleboard prepared with the resin content of 13% and target density of $0.6g/cm^3$ while the deodorization rate was 80~90% for the same particleboard. The formaldehyde emission factor ranged from $0.004mg/m^2{\cdot}h$ to $0.006mg/m^2{\cdot}h$ for all particleboard fabricated at different resin content and target densities. These results indicate that particleboard prepared from the xylem part of Broussonetia Kazinoki Sieb have a potential as a building construction material.

• Journal of the Korean Wood Science and Technology
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• v.38 no.3
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• pp.178-184
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• 2010

This study was carried out to investigate the properties of woodceramics made from woody part of Broussonetia Kazinoki at different impregnation ratios of phenolic resin of 40, 50, 60, 70%. The physical and mechanical properties increased with increasing impregnation ratio. The highest mean values of density, bending strength, Brinell hardness and compressive strength were 0.66 g/$cm^3$, 53 kgf/$cm^2$, 187 kgf/$cm^2$, 126 kgf/$cm^2$, respectively. There were close correlations between density and bending strength, Brinell hardness and compressive strength, and between MOE and MOR.

• YAKHAK HOEJI
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• v.41 no.4
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• pp.439-443
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• 1997

From the root bark of Broussonetia kazinoki (Moraceae) two antioxidant components were identified. Their structures were determined as kazinol A (1) and kazinol E (2) (is oprenylated flavan and isoprenylated 1,3-diphenyl propan derivatives) by comparing NMR data with those of the reported compounds from relative plants. The antioxidant activity of 1 and 2 were monitored by the method of DPPH radical scavenging activity, whose $SC_{50}$ values were $41.4{\mu}M\;and\;33.4{\mu}M$, respectively. These compounds also exhibited inhibitory activity against tyrosinase, which is the sole key enzyme for the melanin biosynthesis and play a role in conversion of tyrosine to dopa, and dopa to dopaquinone. The antioxidant effect and the suppression of melanin biosynthesis are useful for anti-aging, increasing vitality in the incidence of major degenerative diseases, and cosmetic products in relation to hyperpigmentation.