• 한국동물생명공학회지
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• 제38권4호
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• pp.189-198
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• 2023

Background: Brassica oleracea var. italica (broccoli), a rich source of antioxidants, can prevent various diseases and improve human health. In this study, we investigated the antioxidative effects of broccoli sprout extract on oxidative stress induced by lipopolysaccharide and cisplatin in cell and organ tissue models. Methods: Antioxidative effect of BSE was evaluated using DPPH and ABTS in RAW 364.7 cells, and effects of BSE on testes were investigated using Cisplatin-induced testicular damage model with an in vitro organ culture system. Results: The DPPH assay showed that the antioxidant activity of the alcoholic broccoli sprout extract was higher than that of the water extract. Additionally, the expression levels of antioxidation-related genes, Nrf2, Gsr, HO-1, and catalase, were significantly increased in broccoli sprout extract-treated RAW 264.7 cells, and the extract suppressed lipopolysaccharide-induced mitochondrial dysfunction. Based on the results in the RAW 264.7 cell culture, the antioxidative effects of the extracts were investigated in a mouse testis fragment culture. The expression of Nrf2, HO-1, and Ddx4 was clearly decreased in cisplatin-treated mouse testis fragments and not in both broccoli sprout extract- and cisplatin-treated mouse testis fragments. In addition, the oxidative marker O-HdG was strongly detected in cisplatin-treated mouse testis fragments, and these signals were reduced by broccoli sprout extract treatment. Conclusions: The results of this study show that broccoli sprout extracts could serve as potential nutraceutical agents as they possess antioxidant effects in the testes.

• 농업과학연구
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• 제39권1호
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• pp.81-86
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• 2012

In this study, the antioxidative effect and protective potential against oxidative damage of extract and fractions from broccoli were investigated under in vitro and cellular system. The methanol (MeOH) extracts of broccoli flowers and sprouts were partitioned as dichloromethane, n-butanol (BuOH) and aqueous fractions. The comparison of antioxidative effect of broccoli flowers and sprouts showed that broccoli sprouts exerted the more effective protective activity from 2,2'-azobis (2-aminopropane) dihydrochloride (AAPH)-induced oxidative stress in LLC-$PK_1$ porcine renal epithelial cell. In addition, the extract and fractions from broccoli sprouts showed strong scavenging effect of 1,1-diphenyl-2-picrylhydrazyl radical and the BuOH fraction exerted the strongest activity. Therefore, the BuOH fraction was evaluated as the most active fraction with strong radical scavenging activity among the fractions of broccoli flowers and sprouts. The present study suggests the antioxidative potential against free radical-induced oxidative damage of flowers and sprouts of broccoli. In addition, the BuOH fraction of broccoli is considered as the active fraction with antioxidative effect.

• 한국동물생명공학회지
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• 제38권1호
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• pp.17-25
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• 2023

Brassica oleracea var. italica (broccoli) is a type of cabbage that contains vitamins, minerals, and phytochemicals. Consequently, it is used as a potential nutraceutical source for improving human health by reducing oxidative stress and inflammatory responses. Here, the effects of broccoli sprout extract (BSE) on the inflammatory response were investigated through lipopolysaccharide (LPS)-induced inflammatory mouse models. First, we found that the BSE obviously reduce NO production in RAW 264.7 cells in response to LPS stimulation in in vitro study. Pretreatment with BSE administration improved sperm motility and testicular cell survivability in LPS-induced endotoxemic mice. Additionally, BSE treatment decreased the levels of the pro-inflammatory cytokines TNF-a, IL-1β, and IL-6, and COX-2 in testis of LPS-induced endotoxemic mice models. In conclusion, BSE could be a potential nutraceutical for preventing the excessive immune related infertility.

• 한국식품영양학회지
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• 제32권1호
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• pp.1-10
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• 2019

In order to examine the functionality of broccoli sprout (Brassica oleracea, BS), solvent extracts were prepared and their anti-oxidative and immunomodulating activities were compared with those of broccoli (B). EtOH extracts (E) were potently higher than hot-water extracts (HW) in the antioxidant contents and radical scavenging activity. In particular, the total polyphenolic contents in addition to ABTS and DPPH radical scavenging activity were significantly higher in EtOH extract of broccoli sprout (BS-E; 9.15 mg GAE/g, 4.52 mg AEAC/g, and 1.14 mg AEAC/g) compared with that of broccoli (B-E; 7.83 mg GAE/g, 3.63 mg AEAC/g, and 0.97 mg/AEAC/g). Whereas, total flavonoid content was significantly higher in B-E (1.60 mg QE/g) than BS-E (1.43 mg QE/g). Anti-inflammatory activity was investigated using LPS-induced cell line model at a concentration of $10{\sim}100{\mu}g/mL$, in which all solvent extracts of both broccoli sprouts and broccoli were not toxic to RAW 264.7 cell lines. In anti-inflammatory activity of broccoli sprouts, EtOH extracts also showed significantly more potent activity than hot-water extracts in all sample concentrations tested. In addition, BS-E ($100{\mu}g/mL$) inhibited nitric oxide (NO) and IL-6 production to 60.9% and 68.9% compared with the LPS inflammation group (without extracts), whereas B-E inhibited 49.6% and 54.9%. On the other hand, in immunostimulating activity by splenocytes and macrophages, hot-water extract showed significantly higher activity than EtOH extract. Especially, BS-HW stimulated the splenocyte proliferation (1.2-fold against saline group) and IFN-${\gamma}$ production (264.39 pg/mL) at $100{\mu}g/mL$, and the production of IL-6 (1.33-fold), IL-12 (1.09-fold) and TNF-${\alpha}$ (1.49-fold) from macrophages was also significantly enhanced over broccoli. In conclusion, broccoli sprouts showed more potent anti-oxidative, anti-inflammatory and immunostimulating activity than broccoli, suggesting the possibility of using broccoli sprouts as functional food materials.

• 한국식품과학회지
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• 제51권3호
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• pp.207-213
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• 2019

This study was conducted to investigate the antioxidant contents and activities of twelve vegetable sprouts (broccoli, red radish, radish, mizuna, kale, taatsai, pak choi, Chinese cabbage, turnip, rapeseed, chicory, and alfalfa). The total flavonoid contents of the broccoli, red radish, and radish sprout were $25.36{\pm}0.13$, $25.26{\pm}1.80$, and $25.16{\pm}1.25mg$ CE/100 g FW, respectively, and were significantly higher than those of the other tested vegetables. Radish sprouts had the highest total phenolic content (112.42 mg GAE/100 g FW), followed by red radish and broccoli sprouts. The main polyphenols in the vegetable sprouts were epicatechin and chlorogenic acid, but they varied across sprout varieties. The correlation between total flavonoids and total phenolics for the 12 vegetable sprouts was very high (r=0.926). The total antioxidant activity (DPPH and ABTS radical scavenging activities) was also highly correlated with total flavonoids and total phenolics.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 추계국제학술대회
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• pp.78-78
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• 2020

Cruciferous plants such as broccoli and radish contain glucosinolate, which is a bioactive precursor that is most often used in Korean foods and is unique as a food ingredient. In addition, it contains various other phytochemicals and is promising as a health-oriented food material. In particular, Sulforaphane is a hydrolyzate of the glucosinolate, which has a more beneficial effect on the human body. Glucosinolate may be hydrolyzed by enzymes called myrosinase, which is voluntarily possessed by cruciferous plants. However, the ESP(Epithiospecifier protein) in broccoli sprouts could acts competitively with myrosinase, and convert to the less bioactive sulforaphane nitrile form. Therefore, we improved the yielding of sulforaphane in broccoli sprouts with a new method. We induce inactivation of the ESP protein by heat treatment. At this time, a myrosinase was introduced from the radish sprout because myrosinase in broccoli sprouts is also denatured by heat treatment. According to the results, we have confirmed by GC / MS that formation of sulforaphane increases more than 7 fold using set heating and mixing conditions.

• Journal of Biosystems Engineering
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• 제39권4호
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• pp.318-323
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• 2014

Purpose: This study was carried out to predict the growth period and fresh weight of sprouts grown in a cultivator designed to grow sprouts under optimal conditions. Methods: The temperature, light intensity, and amount of irrigation were controlled, and images of seed sprouts were acquired to predict the days of growth and weight from pixel counts of leaf area. Broccoli, clover, and radish sprouts were selected, and each sprout was cultivated in a 90-mm-diameter Petri dish under the same cultivating conditions. An image of each sprout was taken every 24 hours from the 4th day, and the whole cultivating period was 6 days, including 3 days in the dark. Images were processed by histogram inspection, binary images, image erosion, image dilation, and the overlay image process. The RGB range and ratio of leaves were adjusted to calculate the pixel counts for leaf area. Results: The correlation coefficients between the pixel count of leaf area and the growth period of sprouts were 0.91, 0.98, and 0.97 for broccoli, clover, and radish, respectively. Further, the correlation coefficients between the pixel count of leaf area and fresh weight were 0.90 for broccoli, 0.87 for clover, and 0.95 for radish. Conclusions: On the basis of these results, we suggest that the simple image acquisition system and processing algorithm can feasibly estimate the growth period and fresh weight of seed sprouts.

• 한국식품저장유통학회지
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• 제16권2호
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• pp.292-298
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• 2009

본 연구에서는 새싹종자 소독처리 여부에 따른 재배기간별 미생물 오염도 수준을 파악하고, 종자소독이 발아율에 미치는 영향을 분석하였다. 수입산 새싹종자 알팔파(alfalfa), 브로콜리(broccoli), 클로버(clover), 적양배추(red cabbage), 적무(red radish) 5종에 대하여 소독제 처리 여부에 따른 발아율과 재배기간별 미생물 분석을 실시하였다. 소독제 종류에 관계없이 새싹종자(알팔파, 클로버, 적무)는 48시간 후 90% 이상 높은 발아율을 보였다. 종자 소독 시 식중독균은 검출되지 않았고, 대조군에 비하여 유의하게 낮은 미생물 수준을 보였으나, 발아과정에서 일반세균, 대장균군 모두 $10^7-10^8CFU/g$으로 종자소독 여부에 관계없이 종자에 비하여 유의하게 높은 오염 수준을 나타내었다(p<0.05). 또한 종자 소독하지 않은 클로버에서 Listeria monocytogenes 이 검출되었다. 따라서 새싹채소의 위생적인 안전성을 개선하기 위하여 미국 FDA 권고에 따라 재배전 종자의 소독이 필요하며, 발아과정의 HACCP 관리계획을 마련하여 중점적으로 관리하는 것이 필요하겠다.

• 생물환경조절학회지
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• 제16권4호
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• pp.415-419
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• 2007

저장중 5가지 싹채소, (무, 적무, 적양배추, 알팔파, 브로콜리)의 생체중은 $50{\mu}m$ LDPE필름으로 밀폐하여 $2^{\circ}C$와 $8^{\circ}C$모두 99%이상 유지되었다. 저장중 포장재내 이산화탄소 농도는 $8^{\circ}C$에서 $2^{\circ}C$에서 보다 높았으며 작물별로 무와 적무가 높았다. 산소농도는 이와 정반대로 $2^{\circ}C$에서 높게 유지되었고, 무와 적무가 가장 낮은 농도를 보였는데 특히 $8^{\circ}C$저장 3일째는 3%미만까지 낮아지기도 하였다. 에틸렌 농도도 $2^{\circ}C$에 비해 $8^{\circ}C$에서 다소 높았으며 작물별로는 알팔파가 0.1ppm으로 가장 낮았고 나머지 4 작물은 작물간 농도차이에 유의성 없이 1ppm미만으로 낮았다. 이취는 산소농도가 가장 낮았던 무와 적무에서 오히려 낮은 수준을 보였으며 온도별로 큰 차이가 없었다. 외관상 품질은 역시 $2^{\circ}C$가 $8^{\circ}C$보다 높게 유지되었으며, 두 온도 모두에서 무가 가장 높게 유지되었고, 다음으로 적무, 브로콜리, 적양배추, 알팔파 순서였다. 이상의 결과에서 싹채소의 작물간 저장성에 차이를 알 수 있었는데 무와 알팔파의 경우 $4{\sim}5$일의 저장기한의 차이를 보였다. 따라서 싹채소 유통에 있어 작물별 관리가 다르게 실시되어야 할 것이라 생각된다.

• 한국식품영양학회지
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• 제20권4호
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• pp.356-362
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• 2007

This study was carried to develop the new material of functional food which antioxidant of natural substances of sprouts. We compared antioxdiative activity and antioxidant substances exist in sprouts. Antioxidaive activities of sprouts were measured by total polyphenolic acid contents, electron donating activity(EDA), SOD-liked activity and hydroxyl radical scavenging ability. Total polyphenolic acid content was very higher in Rape sprouts extract(Rap) than other sprout extracts. Also Rape extract was showed the most excellent antioxdiative activity in SOD liked activity at 86.94%. The EDA was ordered Rape sprouts extract(Rap)>Tatsoi sprouts extract(Tat)>Broccoli sprouts extract(Bro)>Alfalfa sprout extract(Alf). Hydroxy radical scavenging ability was the most effective in Rape sprouts extract(Rap). Therefore we could be certain that Rape sprouts extract(Rap) was the most effective in antioxidative activity from sprouts.