• 한국임상수의학회지
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• 제23권2호
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• pp.133-143
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• 2006

Diagnosis on reproductive failures of dairy cows by ultrasonography was performed for 151 dairy cows. To diagnose types of reproductive failures, ultrasonography (SA 600, Medison, 5.0 MHz rectal linear transducer) was carried out in combination with rectal examination. Of 151 dairy cows, pregnant cows were 13 and the cows in normal estrual cycle were 40 cows, thereby the cows with reproductive failures were 98 cows. 1. Of 98 cows with reproductive failures, the cows with ovarian diseases were 34 cows (34.7%) and the cows with uterine diseases were 41 cows (41.8%). 2. The diameter of follicle in proestrus was 1.94 cm and it was longer than that of follicle in diestrus (p<0.05). 3. The mean size of corpus luteum of pregnant cows was bigger than that of corpus luteum in normal diestrus (p<0.05). 4. The length of cystic corpus luteum was 3.26 and the width of that was 1.91 cm. The length of corpus luteum tissue was 1.95 and the width of that was 1.91 cm excluding the size of cavity in corpus luteum. 5. The mean length of follicular cyst was 3.31 and the mean width of that was 2.3 cm. 6. The mean length and width of luteal cyst was 3.45 and 2.25 cm, respectively. The mean length and width of corpus luteum tissue was 1.15 and 0.67 cm, respectively, excluding the size of cyst in the luteal cyst. 7. The width of uterine horn associated with endometritis was significantly reduced as the period after parturition was elapsed (p<0.05). The mean width of uterine horn within 40 days after parturition was 4.55 cm. These results indicated that ultrasonography is of great use for accurate diagnosis both on ovarian diseases and uterine diseases and that it is very effective to diagnose endometritis in dairy cows.

• 한국패류학회지
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• 제28권4호
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• pp.335-342
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• 2012

본 연구에서는 31개월령 북방전복의 성장형질에 대한 선발반응을 추정하기 위해 2008년에 생산된 2세대 육종전복의 모집단 6,858마리와 다음 세대 생산을 위해 최종 교배지침에 포함된 614마리의 자료를 이용하였다. 각 형질별 전체평균은 각 장, 각폭 및 중량이 각각 76.31 mm, 49.48 mm 및 56.90 g로 조사되었고, 중량의 변이계수가 28.70%로 나타나 다른 형질에 비해 자료의 변이가 가장 크게 나타났다. 각장, 각폭, 중량 및 비만도에 있어서 성과 성숙도의 효과에 대한 분산분석을 실시한 결과 비만도를 제외한 모든 형질에서 고도의 유의성이 인정되었으며 (p < 0.01), 성의 효과는 비만도를 제외한 모든 형질에 있어서 수컷보다 암컷이 유의적으로 높게 나타났다. 각 형질별 육종가와 표현형가의 상관관계는 각장, 각폭 및 중량에 있어서 순위상관계수와 단순상관계수의 범위가 0.63에서 0.71로 대체적으로 낮게 나타나 육종가와 표현형가의 상관관계를 더 높인다면 보다 효율적인 개량을 도모할 수 있을 것으로 사료되며, 선발반응 예측치 즉, 기대되는 유전적 개량량은 각장, 각폭 및 중량이 각각 9.6%, 9.5% 및 26.1%로 나타났다. 따라서 다음 세대에 있어서 보다 높은 선발반응을 기대하기 위해서는 선발의 정확도는 물론 최종교배를 위해 선발되는 개체의 비율과 선발강도를 적절히 고려하여야 할 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권3호
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• pp.324-328
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• 2006

Carbonic anhydrase III (CA3) is a member of a multigene family that encode carbonic anhydrase isozymes. In this study, a complete coding sequence of the pig CA3 gene which encodes a 260 amino-acid protein was determined. The amino acid comparison showed high sequence similarities with previously identified human (86.5%) CA3 gene and mouse (91.5%) Car3 gene. The partial genomic DNA sequences were also investigated. The length of intron 1 was 727 bp. Comparative sequencing of three pig breeds revealed that there was a T${\rightarrow}$C substitution at position 363 within intron 1. The substitution was situated within a NcoI recognition site and was developed as a PCR-restriction fragment length polymorphism (RFLP) marker for further use in population variation investigations and association analysis. Two alleles (A and B) were identified, and 617 bp fragments were observed for the AA genotype and 236 bp and 381 bp fragments for the BB genotype. The polymorphism of CA3 was detected in 8 pig breeds. Allele B was predominant in the Western pig breeds. In addition, association studies of the CA3 polymorphism with carcass traits in 140 $Yorkshire{\times}Meishan$ $F_2$ offspring showed that the NcoI PCR- RFLP genotype may be associated with variation in several carcass traits of interest for pig breeding. Allele B was associated with increases in lean meat percentage, loin eye height and loin eye area. Statistically significant association with backfat thickness was also found; pigs with the AB genotype had much less backfat thickness than AA or BB genotypes.

• 한국수산과학회지
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• 제56권4호
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• pp.380-387
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• 2023

Stocking density is associated with Paralichthys olivaceus growth; thus, fish should be rapidly reared at high densities for commercial reasons. Studies have reported that high stocking density retards growth; however, few have investigated the malformations caused by stocking density stress. This study compared the growth and malformation rates of P. olivaceus at different densities and stress- and immune-related gene expression between malformed and normal fish. Forty days post-hatching, fish (total length, 1.49±0.02 cm) were reared at 800 (low density; LD), 1500 (medium density; MD), and 4000 (high density; HD) fish/m², and the growth rate was measured weekly. On day 30, RNA was extracted from the kidneys, and the expression of stress-, immune-, and malformation-related genes was analyzed using qRT-PCR. The malformation rate in the HD groups was approximately three times higher (62%) than that in the LD and MD groups (approximately 20%), and growth was lower regarding length and weight. The stress-related (HSP70 and GPX) and immune-related (PIR and IgM) genes showed higher mRNA expression in the HD group and malformed fish than in the LD group and normal fish. However, TLR3 showed the opposite results. In summary, high stocking density suppressed growth and increased malformation risk in P. olivaceus.

• Plant Resources
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• 제8권3호
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• pp.250-257
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• 2005

This study was performed to provide basic information of the development and breeding of new cultivars of Hibiscus syriacus L., which have more beautiful and diverse flowers. Morphological characteristics of the flowers and leaves, and genetic variation of the flowers color of two Jeoktanshim-line cultivars, Bulsae and Pyungsung, were crossed each other. The result of the cross between Bulsae and Pyungsung are as follows: Mean flower height and width were 5.35 cm and 7.69 cm, respectively. Mean length and width of petal were 5.43 cm and 3.80 cm respectively, and mean length and width of flower pistil were 4.67 cm and 0.44 cm, respectively. The flower color of all ten individuals was the color of the Jeoktanshim-line, and Pyungsung, and all flower type were I-c type.

• 한국작물학회지
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• 제45권6호
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• pp.374-381
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• 2000

This study was conducted to select sesame varieties with high cultural stabilities by comparing several parameters of agronomic traits under the different cultural environments. Of the six areas, Iksan and Jinju areas which showed positively larger environment index values were relatively adequate cultural conditions for sesame. At the comparison of cultural stability of agronomic traits by Eberhart and Russell regression model among sesame breeding lines, Suwon 169 showed more stable regression coefficient values to the number of capsules per plant, number of seeds per capsule and seed weight per plant, and Iksan 12 showed more stable regression coefficient values to culm length and weight per plant. At the comparison of cultural stability of yield per 10a, Suwon 169 and Iksan 12 among sesame breeding lines showed more stable respectively, deviation values of 0.99, 0.98 respectively, and more less regression deviation values of 0.074, 0.167 respectively. Therefore those breeding lines are comparatively higher stabilities to yield determining agronomic traits under the different cultural environments, and it was concluded that those two breeding lines had the possibility to recommend promising breeding lines in the future.

• 한국산림과학회지
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• 제90권4호
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• pp.445-449
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• 2001

가로수용 무궁화를 만들기 위한 무궁화 수행유도 실험에서 무궁화의 지하고를 100cm, 150cm, 200cm로 하고 시비를 달리한 결과, 각각의 근원경 평균이 15.7mm, 13.8mm, 10.8mm로 지하고가 낮을수록 근원경의 생장이 우수하였으며, 분산분석의 결과도 지하고에 따른 영향이 유의한 것으로 나타났다. 그리고 시비에 따른 영향은 유의하지 않았다. 또한 가로수로 식재하여 '99년부터 2000년까지 2년 동안 조사한 결과는 지하고 80~120cm, 121~150cm, 151-200cm에서 각각의 근원경 평균이 4.9mm, 4.5mm, 3.0mm의 값을 나타내 지하고 80~120cm, 121~150cm의 근원경 생장이 좋은 것으로 나타났다. 앞으로 가로수용으로 무궁화를 육성하가 위해서는 먼저 수고와 지하고를 고려하는 것이 타당한 것으로 본 실험의 결과에서 나타났다. 그러나 본 실험의 결과는 짧은 기간의 시험에 의한 것이므로 앞으로 계속하여 관찰 연구하여 보완하고자 한다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권1호
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• pp.13-18
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• 2006

Two different animal models, which differ in whether or not taking maternal genetic effect into account, for estimating genetic parameters of cashmere weight, live body weight, cashmere thickness, staple length, fiber diameter, and fiber length in Inner Mongolia White Cashmere Goat were compared via likelihood ratio test. The results indicate that maternal genetic effect has significant influence on live body weight and cashmere thickness, but no significant influence on the other traits. Using models suitable for each trait, both genetic parameters and trends were analyzed with the MTDFREML program. Heritability estimates from single trait models for cashmere weight, live body weight, cashmere thickness, staple length, fiber diameter and fiber length were found to be 0.30, 0.07, 0.21, 0.29, 0.28 and 0.21, respectively. Genetic correlation estimates from two-trait models between live body weight and all other traits (-0.06~0.07) was negligible, as were those between fiber diameter and all other traits (-0.01~0.03) except cashmere thickness (0.19). Cashmere weight and staple length had moderate to low genetic correlations with other traits (-0.24~0.39 and -0.24~0.34, respectively) except for live body weight and fiber diameter. Cashmere thickness had a strong genetic correlation with fiber length (0.81), and low genetic correlation with other traits (0.19~0.34) except live body weight. Genetic trend analysis suggests that selection for cashmere weight was very effective, which has led to the slow genetic progress of cashmere thickness and fiber length due to their genetic correlations with cashmere weight. The selection for live body weight was not effective, which was consistent with its low inheritability.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1995년도 제9회 식물생명공학 심포지움 식물육종과 분자생물학의 만남 The 9th Plant Biotechnology Symposium -Breeding and Molecular Biology-
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• pp.133-170
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• 1995

The major objectives of fruit breeding lie in improvement of cultivar, easy to be cultivated and of high quality, in order to produce unexpensive, delicious fruit both for fresh fruit market and processing. Recently, fruit breeding in Korea has contributed to breeding of several superior cultivars in major fruit crops, resulting in appreciable improvement in qualities such as skin color, taste and fruit-bearing habit concerned with productivity. In spite of accomplishments mentioned above, the need for both highly disease-resistant cultivars and long-keeping, physiological disorder-resistant cultivars to meet long distance transsportation in the temperate fruit crops of apples, oriental pears, stone fruits such as peaches, and grapes grown in Korea is rapidly pressing more than ever, as cultivars of today susceptible to pests and diseases and vulnerable to physiological disorders are very expensive and time-consuming in post-harvest handling and management. Thus, imporvements made in the above problems through breeding level will lead to the really enhanced productivity in fruit industry. The major impediments of tree size, the long length of juvenile period and the highly heterogeneous genetic composition to the improvement of fruit crops are responsible for the lower amount and rate of improvements of fruit crops as compared to annuals. Considering the expected limitations of the above problems to be solved through conventional breeding methods and strategy, a turning point of breeding a near perfect cultivar would be laid down if innovative breakthroughs in biological technology will be realized in applying some of the techniques of genetic manipulation at the molecular level to the cultivar improvement of fruit crops, such as the selective insertion of DNA carrying genes that govern desirable characteristics. More than anything else, those traits such as fruiting habit deciding productivity, elements of fruit qualities conditioned by monogene, and disease-and pest-resistance of vital importance for successful fruit growing are urgently desired to be improved by advancement of biotechnology for they are more than difficult and need long period to be attained through conventional breeding method.

• Asian-Australasian Journal of Animal Sciences
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• 제20권10호
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• pp.1473-1477
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• 2007

Four-and-a-half LIM-only protein 3 (FHL3) is a member of the LIM protein superfamily and can participate in mediating protein-protein interaction by binding one another through their LIM domains. In this study, the 5'- and 3'- cDNA ends were characterized by RACE (Rapid Amplification of the cDNA Ends) methodology in combination with in silico cloning based on the partial cDNA sequence obtained. Bioinformatics analysis showed FHL3 protein contained four LIM domains and four LIM zinc-binding domains. In silico mapping assigned this gene to the gene cluster MTF1-INPP5B-SF3A3-FHL3-CGI-94 on pig chromosome 6 where several QTL affecting intramuscular fat and eye muscle area had previously been identified. Transcription of the FHL3 gene was detected in spleen, liver, kidney, small intestine, skeletal muscle, fat and stomach, with the greatest expression in skeletal muscle. The A/G polymorphism in exon II was significantly associated with birth weight, average daily gain before weaning, drip loss rate, water holding capacity and intramuscular fat in a Landrace-derived pig population. Together, the present study provided the useful information for further studies to determine the roles of FHL3 gene in the regulation of skeletal muscle cell growth and differentiation in pigs.