• Journal of the Korean Chemical Society
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• v.53 no.6
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• pp.742-748
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• 2009

In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. We described the development of DNA markers to discriminate between Korean beef cattle (Hanwoo), Holstein, and mixed cow beefs. As most breeds are standardized for coat colour, the melanocortin 1 receptor (MC1R) gene, involved in the regulation of eu/pheomelanins synthesis, has been suggested as marker for breed traceability of products of animal origin. We also designed sex-determining region Y (SRY) gene specific primers for Y chromosome detection. In this study, fragments of MC1R gene and SRY gene were amplified by multiplex-PCR and subjected to digestion by MspA1I restriction endonuclease. Reaction products were analysised by denaturing high performance liquid chromatography (DHPLC). As a result, we identified 6 DHPLC peak types from MC1R gene and SRY gene analysis. DHPLC method showed more sensitive than RFLP method for DNA fragments analysis. Therefore, DHPLC method can apply to identify for Hanwoo, Holstein and mixed beef.

• Journal of Veterinary Clinics
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• v.30 no.6
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• pp.503-505
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• 2013

A cross breed dog (6-year-old, 6 kg, intact male) was referred with hematuria. The dog had been treated for years owing to the urinary bladder calculi. On abdominal radiography, prostatic calculus was demonstrated in the prostatic area. In addition, ultrasonography and computed tomography (CT) scan would confirm that the part of calculus protruded within the prostatic urethra. The patient underwent a prostatolithotomy and traumatic prostatic urethra was carefully sutured and the omentum was filled with the prostate lumen. A crystallographic analysis of the stone showed 80% magnesium ammonium phosphate (struvite) and 20% carbonate apatite. The leakage of the urine was not observed post-operation and the hematuria improved and there was no specific problem at the 6 months follow-up.

• Korean Journal of Veterinary Service
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• v.20 no.1
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• pp.47-53
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• 1997

We have investigated the prevalence of canine heartworm (Dirofilaria immitis) infection among 100 mixed-breed dogs raised in Chonnam Province (Koheung, Posung, Changheung, and Hwasoon) and 100 dogs randomly selected from visiting dogs to the College of Veterinary Medicine, Chonnam National University for routine physical examination. Blood samples taken from dogs were examined for the presence of Dirofilaria immitis microfilaria by the modified Knott's test, and an antigen test which was based on the sandwich ELISA(DiroCHEK(equation omitted), Synbiotics, Inc., San Diego, California, USA) was used to detect the adult heartworm-specific antigen from serum samples. The results were then compared with those of the modified Knott's test. Five(2.5%) of the 200 examined dogs were microfilaria-positive, while 8 dogs(4%) were anti-gen-positive, which suggested that the antigen test was more sensitive than the microfilarial test in detecting heartworm infection. All dogs that were microfilaria-positive were also antigen-positive. The place of origin of the heartworm-positive dogs were of Naju (4/26, 15.4%), Koheung(3/32, 9.4%), and Hwasoon (1/19, 5.3%), while none of dogs raised in Kwangju, Posung, and Changheung areas were infected with the parasite. Although not significant, more infected dogs were observed in older ages, while the difference in infection rate between male (4/98, 4.1%) and female (4/102, 3.9%) was not recognized. Our results indicated that a relatively low infection rate was observed in dogs raised in Kwangju and Chonnam area compared to the infection rate reported previously in other areas of South Korea.

• Horticultural Science & Technology
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• v.34 no.5
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• pp.761-770
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• 2016

This study investigated the variation in fruit characteristics between different triploid progenies derived from a cross between tetraploid and diploid grape cultivars, with the aim of developing high quality, labor-saving triploid grapes. No differences were found in fruit quality traits such as soluble solids content and titratable acidity, whereas significant variation was noted in cluster weight induced by a one-time application of GA3 (100 ppm) on the triploid progeny of different cross combinations. The cluster weight was higher in progeny obtained from 'Kyoho' ${\times}$ 'Sekirei' than for offspring derived from other cross combinations; deviation in the mean cluster weight was also small for 'Kyoho' ${\times}$ 'Sekirei' progeny. These results suggest that specific cross combinations, such as 'Kyoho' ${\times}$ 'Sekirei', can be used to efficiently breed new, labor-saving seedless grape cultivars.

• International Journal of Industrial Entomology and Biomaterials
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• v.4 no.1
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• pp.23-30
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• 2002

The general and specific combining abilities and hybrid vigour of seven polyvoltine breeds [BL24, BL62, BL6S, BL67, BL68, BL69, and Pure Mysore (PM)] with five bivoltine breeds (NB4D2, CSR2, CSR5, CSR18 and CSR19) were evaluated in a line ${times}$ tester design by analysing six quantitative traits viz., fecundity, yield 110,000 larvae both by number and weight, cocoon weight, shell weight and cocoon shell ratio. ANOVA estimates showed significant effects for all the traits indicating the role of both additive and non- additive gene actions. Estimation of GCA revealed that the breed BL67 exhibited significant positive effects toy all the six traits followed by BL68 for four important economic traits viz., fecundity, cocoon weight, shell weight and cocoon shell ratio. Among testers, CSR5 showed significant GCA effects for five traits whereas CSR2 exhibited for shell weight and shell ratio. Three hybrids viz., BL67${times}$ NB4D2, BL67 ${times}$CSR5 and BL67${times}$ CSR19 excelled in four quantitative traits for SCA. Majority of the hybrids expressed positive hybrid vigour for cocoon yield/10,000 larvae by weight, cocoon weight and shell weight. Four hybrids namely PM ${times}$ NB4D2, BL67 ${times}$NB4D2, BL67${times}$ CSR5 and BL67${times}$ CSR19 manifesting positive hybrid vigour for five economic traits were adjudicated as best Heterotic hybrids and recommended for commercial exploitation to increase the silk yield.

• Journal of Contemporary Eastern Asia
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• v.18 no.2
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• pp.16-32
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• 2019

Media manipulation of breaking news through article selection, ranking and tweaking of social media data and comment streams is a growing concern for society. We argue that the combination of human and machine curation on media portals marks a new period for news media and journalism. Although intermediary platforms routinely claim that they are merely the neutral technological platform which facilitates news and information flows, rejecting any criticisms that they are operating as de facto media organisations; instead, we argue for an alternative, more active interpretation of their roles. In this article we provide a contemporary account of the South Korean ('Korean') online news media ecology as an exemplar of how contemporary media technologies, and in particular portals and algorithmic recommender systems, perform a powerful role in shaping the kind of news and information that citizens access. By highlighting the key stakeholders and their positions within the production, publication and distribution of news media, we argue that the overall impact of the major portal platforms of Naver and Kakao is far more consequential than simply providing an entertaining media diet for consumers. These portals are central in designing how and which news is sourced, produced and then accessed by Korean citizens. From a regulatory perspective the provision of news on the portals can be a somewhat ambiguous and moving target, subject to soft and harder regulatory measures. While we investigate a specific case study of the South Korean experience, we also trace out connections with the larger global media ecology. We have relied on policy documents, stakeholder interviews and portal user 'walk throughs' to understand the changing role of news and its surfacing on a distinctive breed of media platforms.

• Journal of Veterinary Clinics
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• v.30 no.5
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• pp.390-393
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• 2013

This case study describes the imaging features of radiography and computed tomography (CT) in four small breed dogs with lung lobe torsion. The medical records, thoracic radiographs and CT images of four dogs with confirmed lung lobe torsion were retrospectively reviewed. Pleural effusion and increased lobar opacity/density were seen on all CT and thoracic radiography in all four dogs. CT revealed narrowed/collapsed bronchus in all four dogs, while this was only appreciated on one thoracic radiography. The vesicular emphysema pattern was seen on four CT but only on three radiographic examinations. The specific findings of lung lobe torsion were vesicular emphysema and a narrowed/collapsed bronchus. These findings were more easily recognized on CT than with thoracic radiographs.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.12
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• pp.1791-1797
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• 2007

A study was conducted with 70 Hanwoo (Korean cattle) for genotyping bovine leukocyte antigen (BoLA)-DRB3.2 gene by using the polymerase chain reaction (PCR) and sequence based typing (SBT). Two-step PCR was carried out for amplifying a 284 bp fragment of the target gene and the PCR products were digested with three restriction enzymes namely RsaI, BstYI and HaeIII. Seventeen alleles were detected with frequencies ranging from 1.43 to 18.57% and one (x'aa) of these alleles was identified as a new allele that has not been reported before. The frequency of the new x'aa allele identified in this breed was 12.86%. In addition, the seven most frequently observed alleles (DRB3.2 *10, *15, *16, *26, *27, *54 and x'aa) accounted for 74.28% of the alleles in this population. The phylogenetic tree showed that the BoLA-DRB3.2 allele sequences of Hanwoo were shared with other Bos taurus breeds and no specific clade for Hanwoo was identified. It indicates high heterogeneity of the BoLA-DRB3 gene in this population and may give some ideas for breeding animals having better disease resistance.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.5
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• pp.603-607
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• 2004

Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) analysis was carried out with a battery of 11 random decamer primers to study band frequency (BF), genetic identity index (I) and mean average percentage difference (MAPD) between Bhadawari and Murrah breeds of buffalo. The primers OPA04 and BG15 resolved a band of 460 bp, which was present only in animals of Bhadawari breed. Whereas, the primers OPA14, BG27 and BG28 produced Murrah specific fragments of sizes 730 bp and 1,230 bp, respectively. The estimate of genetic identity index was highest (0.845) with the primer OPA01 and the lowest (0.479) with the primer BG27. The genetic identity index pooled over the primers was 0.596${\pm}$0.037 between these two breeds. The highest MAPD estimate (53.9) between the two breeds was obtained with the primer BG27 and the lowest (14.3) with the primer OPA01. It might be concluded that the genetic identity index between these two breeds calculated on the basis of BF showed moderate level of genetic identity with the primers employed. MAPD calculated on the basis of uncommon bands also demonstrated lower to medium level of genetic difference between Bhadawari and Murrah breeds of buffalo.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.6
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• pp.895-902
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• 2012

The Korean native chickens (Woorimotdak$^{TM}$, KNC) and commercial broilers (Ross, CB) show obvious differences in meat flavor after cooking. To understand the contribution of protein and peptide for meat flavor, 2-dimensional (2-D) gel electrophoresis and matrix-assisted laser desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry was performed. A total of 16 protein spots were differentially expressed in the breast and thigh meat between the two breeds. A total of seven protein spots were represented by different levels between KNC and CB for breast meat. Among them three protein spots (TU39149, TU40162 and TU39598) showed increases in their expressions in KNC while other four protein spots (BU40125, BU40119, BU40029 and BU39904) showed increases in CB. All nine protein spots that were represented by different levels between KNC and CB for thigh meat showed increases in their expression in KNC. Phosphoglucomutase 1 (PGM 1), myosin heavy chain (MyHC), heat shock protein B1 (HSP27), cytochrome c reductase (Enzyme Q), Glyoxylase 1, DNA methyltransferase 3B (DNA MTase 3) were identified as the main protein spots by MALDI-TOF mass spectrometry. These results can provide valuable basic information for understanding the molecular mechanism responsible for breed specific differences in meat quality, especially the meat flavour.