• YAKHAK HOEJI
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• v.51 no.4
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• pp.259-263
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• 2007

We previously reported the isolation of a sialic acid-specific lectin eluted from the bark of Maackia fauriei using alkaline buffer on a fetuin-affinity column. Application of a borate-based elution buffer in the present study increased the specific activity of purified lectin from crude protein extract by 2.6-fold, whilst only slightly decreasing the recovery by 1.13%. The biological properties of the lectin eluted with borate buffer were the same as those of the lectin eluted with alkaline buffer such as in terms of the hemagglutination activity, hemagglutination inhibition activity, molecular mass, purity, and cytotoxicity to human breast cancer cells. A prepared biotin-labeled lectin conjugate was used to investigate the binding to various glycoproteins. Our results indicate that eluting with borate buffer is more efficient than using alkaline buffer to isolate the lectin adsorbed in a fetuin-affinity column.

• Korean Journal of Head & Neck Oncology
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• v.18 no.2
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• pp.203-206
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• 2002

Metastatic carcinomas to the thyroid gland are rare, and thyroid involvement by secondary carcinomas commonly results from direct the extension of malignant cells from adjacent organs such as the larynx or the trachea. The common primary sites of thyroid metastasis are kidney, breast, lung, and lymphoid tissue. Among head and neck cancers, nasopharyngeal carcinoma has a relatively high incidence of distant metastases to other sites and commonly involving sites are bone, lung, and liver. Recently, we experienced a case of a 43-year-old male who had been presented with neck mass for 3 months. He was diagnosed non-keratinizing nasopharyngeal carcinoma in 1993. And, thyroid metastasis of nasopharyngeal carcinoma was confirmed by total thyroidectomy. So we report this rare case with the review of literatures.

• IMMUNE NETWORK
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• v.9 no.1
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• pp.4-7
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• 2009

Although the success of trastuzumab and rituximab for treatment of breast cancer and non-Hodgkins lymphoma, respectively, suggests that monoclonal antibodies(mAbs) will become important therapeutic agents against a wider range of cancers, useful therapeutic Abs are not yet available for the majority of the human cancers because of our lack of knowledge of which antigens (Ags) are likely to become useful targets. We established a procedure for comprehensive identification of such Ags through the extensive isolation of human mAbs that may be therapeutic. Using the phage-display Ab library we isolated a large number of human mAbs that bind to the surface of tumor cells. They were individually screened by immunostaining, and clones that preferentially and strongly stained the malignant cells were chosen. The Ags recognized by those clones were isolated by immunoprecipitation and identified by mass spectrometry(MS). We isolated 2,114 mAbs with unique sequences and identified 25 distinct Ags highly expressed on several carcinomas. Of those 2,114 mAbs 434 bound to specifically to one of the 25 Ags. I am going to discuss how we could select proper target Ags for therapeutic Abs and candidate clones are therapeutic agents.

• Journal of the Korean Society of Clothing and Textiles
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• v.35 no.4
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• pp.442-454
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• 2011

This study analyzed the present uniform satisfaction, uniform satisfaction followed by physical satisfaction, attitude for uniform, purchase attitude for uniform, satisfaction for design, preference for design, and attitude for uniform alteration behavior by distributing questionnaires to high school girl students in Daejeon City. The results of the study are as follows. The study distributed questionnaires to 319 high school girl students in Daejeon City and analyzed the satisfaction factors for their uniforms. As the result, this study induced four factors. The average value of each factor appeared in order of symbolic satisfaction, management satisfaction, activity satisfaction, and aesthetic satisfaction to show that high school girl students were the most dissatisfactory in aesthetic satisfaction. When the study examined the realities of uniform alteration, it showed that 71.2% of them altered their uniforms. In the alteration of a jacket and a skirt, they altered their jackets shortly and tightly in the mass in the order of: waist measurement, jacket length, breast width, shoulder width, and girth of the chest in a jacket. For a skirt, they altered their skirts tightly as a jacket in the order of: skirt length, waist measurement, and the girth of hip.

• The Korean Journal of Cytopathology
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• v.12 no.1
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• pp.49-52
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• 2001

Salivary duct carcinoma is an uncommon aggressive malignant epithelial neoplasm with similarity to intraductal carcinoma of the breast. This neoplasm occurs most often in the parotid gland of middle-aged and older males. About 7% of reported tumors occured in the submandibular gland. The report of salivary duct carcinoma with calcification is rare. We report a case of salivary duct carcinoma with calcification in the submandibular gland. The patient was a 73-year-old male with a mass of the right submandibular gland for 1 year. On the fine needle aspiration cytology, the aspirate showed scant cellularity, small clusters of tumor cells, and scattered small calcifications. Nuclei of the tumor cells showed mild pleomorphism and round to oval in shape, and cytoplasm was abundant and finely granular. Nucleoli were indistinct and necrosis was not noted. There were no cribriform or papillary arrangements of tumor cells. Cytologlc findings of salivary duct carcinoma are variable depending on histologic findings, and calcifications could be an additional cytologic findings.

• The Korean Nurse
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• v.35 no.5
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• pp.98-109
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• 1997

The purpose of this study is to identifY the problems and factors which are closely related to the breastfeeding practice in Korea. The subjects are breastfeeding mothers who have participated in the First Healthy Breastfeeding Child Contest. The 50.8% of subjects are resided in Seoul. The 68.3% of subjects are in their twenties and the 50% of them are high school graduates. Also, 81.4% of the subjects have no job. The 50% of subjects consider that the breastfeeding practice is good for the physical development and health of the child. The 73.9% of the subjects believe that the promotion of breastfeeding practice can be achieved through breastfeeding education through mass media. The 50.4% of subjects consider that the appropriate length of breastfeeding and duration is 9 months. Subjects did proper care of their breast and nipples and did proper measures for the breastfeeding practice. The 95.8% of subjects practiced breastfeeding voluntarily and their breastfeeding practice was not inhibited through factors which were given in the questionnaire lists. In conclusion, the practice of breastfeeding is mostly dependent on the efforts and the positive attitude of breastfeeding mothers. Also the friendly breastfeeding environments in terms of social norms and social practice are the most pertinent factors in promotion of breastfeeding practice.

• Clinical and Experimental Pediatrics
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• v.57 no.11
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• pp.500-504
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• 2014

Kaposiform hemangioendothelioma (KHE) is a rare, locally aggressive vascular tumor of intermediate malignancy with resemblance to Kaposi sarcoma. It occurs predominantly in pediatric age groups as a cutaneous lesion with focal infiltration into the adjacent soft tissue and bone. Although visceral involvement is very uncommon, several cases with bone, retroperitoneal, or mediastinal involvement have been described. KHE has been reported to occasionally occur in unusual sites such as the thymus, tonsils, larynx, paranasal sinuses, deltoid muscle, spleen, uterine cervix, thoracic spine, and even the breast. Multifocal KHE is an extremely rare entity with few reports available in the literature, none of which describes pulmonary involvement. Herein, we report a unique case of multifocal KHE in a 13-year-old boy presenting with a huge soft tissue mass in the upper extremity complicated by bilateral pulmonary nodules that developed into large, necrotic tumor masses.

• Journal of Chest Surgery
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• v.15 no.1
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• pp.118-123
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• 1982

Fourteen cases of pericardia I tumor were clinically experienced from June 1966 to July 1981, for 15 years in St. Mary's Hospital, Department of Thoracic and Cardiovascular Surgery, Catholic Medical College. There were three primary tumors of the pericardium, liposarcoma, teratoma and malignant mesothelioma, and 11 metastatic pericardial tumors. In metastatic pericardial tumors, eight were originated from the lung, one was breast, and the other two cases were unknown origin. There were 6 adenocarcinoma, one small cell carcionoma ~nd one alveolar cell carcinoma in 8 cases from the lung, and 5 male and 3 female patients were composed the metastatic pericardial cancer from the lung. In clinical symptoms were dyspnea in all cases, and 9 cases had the generalized edema and enlarged liver size. Six patients had been operated, three of the primary pericardial tumor and three of metastatic pericardial tumor. Two of the primary tumors were cured satisfactorily by the mass removal, but one died due to cardiac arrest at postoperative one day. In metastatic tumors, operation were performed as two pericardial window formation and one left lower lobectomy with pericardial fenestration, but one was died in second operative day. Other nine metastatic tumors were diagnosed by needle biopsy in one case and by cell block of effusion in eight cases.

• Journal of Chest Surgery
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• v.35 no.10
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• pp.768-772
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• 2002

Sinus histiocytosis with massive lymphadenopathy or Rosai-Dorfman disease(RDD) is a rare type of histiocytosis syndrome, that presents in its most typical form as massive, painless, bilateral lymph node enlargement in the neck during the first or second decade of life. The disease involves extranodal site in over 25% to 43% of the cases, however cases of extranodal RDD without nodal disease have rarely been reported. The involved sites of extranodal RDD have been reported various that were orbit, ocular adnexae, head and neck, upper respiratory tract, breast, gastrointestinal tract, CNS, etc. A 35 year-old man was present with pleural pain on left anterior chest and anterior mediastinal mass, that underwent an excision to remove the suspected invasive thymoma, and the diagnosis was confirmed to extranodal Rosai-Dorfman disease.

• The Korean Journal of Physiology and Pharmacology
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• v.13 no.1
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• pp.49-54
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• 2009

The mushroom Cordyceps militaris has been used for a long time in eastern Asia as a nutraceutical and in traditional Chinese medicine as a treatment for cancer patients. In the present study, a cytotoxic antifungal protease was purified from the dried fruiting bodies of C. militaris using anion-exchange chromatography on a DEAE-Sepharose column. Electrophoretic analyses indicated that this protein, designated C. militaris protein(CMP), has a molecular mass of 12 kDa and a pI of 5.1. The optimum conditions for protease activity were a temperature of $37^{\circ}C$ and pH of $7.0{\sim}9.0$. The enzyme activity was specifically inhibited by the serine protease inhibitor phenylmethylsulfonyl fluoride. Amino acid composition of intact CMP and amino acid sequences of three major peptides from a tryptic digest of CMP were determined. CMP exerted strong antifungal effect against the growth of the fungus Fusarium oxysporum, and exhibited cytotoxicity against human breast and bladder cancer cells. These results indicate that C. militaris represents a source of a novel protein that might be applied in diverse biological and medicinal applications.