• 대한통합의학회지
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• 제9권1호
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• pp.23-31
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• 2021

Purpose : To compare the immediate effects of air stacking maneuver using resuscitator bags and balloons. Methods : Twenty healthy young adults participated in this study. Forced vital capacity (FVC) and peak cough flow (PCF) tests were performed at pre-intervention, and then, the maximum insufflation capacity (MIC), forced expiratory volume in 1 s (FEV1), FEV1/FVC, peak expiratory flow (PEF), and peak cough flow (PCF) were measured using the air stacking maneuver via resuscitator bags and balloons. Interventions were randomly performed, and a 40-min break was provided between interventions. The evaluation process in this study was conducted in accordance with the guidelines of the American Thoracic Society (ATS) 2019. To compare the three outcomes measured at pre-and post-interventions, repeated measures analysis of variance was performed. Results : A significant difference was found in the MIC, FEV1, PEF, and PCF after the air stacking maneuver using resuscitator bags and balloons, whereas no significant difference was observed between resuscitator bags and balloons. Conclusion : No significant difference was found in the immediate effect of the air stacking maneuver using resuscitator bags and balloons in this study. Air stacking maneuver using balloons can increase the success rate of the techniques by providing visual feedback on the amount of air insufflation when performed with balloon blowing exercise. Balloons are cheaper and easier to buy compared to manual resuscitator bags; therefore, education on the air stacking maneuver using balloons will have a positive effect on pulmonary rehabilitation.

• Ecology and Resilient Infrastructure
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• 제7권4호
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• pp.274-283
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• 2020

도로포장 절단 작업에서 미세먼지를 많이 발생시키고 있어, 이 작업에서 미세먼지 발생량을 줄이는 것이 전체 미세먼지 관리에 매우 중요하다. 이러한 점에 착안하여, 본 연구에서는 도로절단 현장에서 발생하는 비산먼지를 회수하는 장치의 개념설계를 제안하고, 이의 도입에 대한 경제적 타당성을 분석하였다. 제안된 개념모델의 경제성을 분석한 결과, 편익비율이 2.96으로 1을 크게 상회하였다. 그리고 수익률은 62.78%로 도로포장 절단 실무자 면담을 통해 설정한 최소기대수익률 10%를 크게 상회하였으며, 손익분기점은 약 21.6개월로 분석되었다. 따라서 제안된 저소음·분진회수형 도로절단 장비의 경제적 타당성은 충분할 것으로 예상된다.

• 한국노년학
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• 제29권3호
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• pp.1087-1105
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• 2009

본 연구는 황혼이혼이 점차 증가하고 있는 현 시점에서 황혼이혼을 한 여성노인들의 이혼결정과 이혼 이후의 적응과정 및 내용을 살펴보고, 황혼이혼 여성 노인들의 구체적 삶에 대한 이해의 기반을 조성해 그들의 적응을 도울 수 있는 실천적 개입 프로그램 마련에 기여하고자 수행되었다. 연구는 질적 연구 방법론들 중 하나 인 생애사 연구로 접근했다. 연구에는 3명의 황혼이혼여성 노인들이 참여했고 일대일 심층면담을 통해 자료를 수집했다. 수집된 자료는 Denzin(1989)이 제시한 분석방법을 원용하여 범주적 내용분석을 했다. 연구결과에서는 참여자들의 이혼 전·후의 주요 생애주제와 이혼 이후의 삶 적응을 분석하여 공통의 주제를 발견했는데 그것은 '문화체계로 굳어진 가부장제'와 '편견과 억압속의 분절된 삶'이다. 이를 통해 본 연구에서는 황혼이혼 여성노인들을 위한 자조모임과 특화된 개입프로그램의 필요성을 제언하고자 하였다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2022년도 추계학술대회
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• pp.570-573
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• 2022

본 논문에서는 신경망 모델을 적용한 몰드변압기의 보이드 결함 크기 판별에 관한 연구를 수행하였다. PCB 기반의 로고우스키 코일형 부분방전 센서를 제작하여 부분방전 신호를 측정하였고, 보이드에 의한 부분방전 결함을 모의하기 위한 PD 전극계를 제작하였다. 또한 보이드는 원통형 모양의 알루미늄 틀을 제작하여 에폭시가 경화되는 과정에서 실린지를 삽입하고 공기를 주입하여 서로다른 직경을 가지는 4개의 시편을 제작하였다. 보이드 결함 크기 판별을 위해 부분방전 전하량, 방전 펄스 수, 위상 분포의 부분방전 특성 파라미터를 추출하여 Labview 기반의 VI (Virtual Instrument)로 역전파 알고리즘을 설계하였다. 실험 결과로부터 제작된 알고리즘은 90%이상의 판별률로 결함의 직경크기를 구분할 수 있었다. 본 연구의 결과는 현장에서 PD 측정 시 몰드변압기의 유지보수 및 절연물 교체의 근거 자료로 활용될 수 있을 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제33권9호
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• pp.1228-1237
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• 2023

The CRISPR-Cas system has emerged as the most efficient genome editing technique for a wide range of cells. Delivery of the Cas9-sgRNA ribonucleoprotein complex (Cas9 RNP) has gained popularity. The objective of this study was to develop a quantitative polymerase chain reaction (qPCR)-based assay to quantify the double-strand break reaction mediated by Cas9 RNP. To accomplish this, the dextransucrase gene (dsr) from Leuconostoc citreum was selected as the target DNA. The Cas9 protein was produced using recombinant Escherichia coli BL21, and two sgRNAs were synthesized through in vitro transcription to facilitate binding with the dsr gene. Under optimized in vitro conditions, the 2.6 kb dsr DNA was specifically cleaved into 1.1 and 1.5 kb fragments by both Cas9-sgRNA365 and Cas9-sgRNA433. By monitoring changes in dsr concentration using qPCR, the endonuclease activities of the two Cas9 RNPs were measured, and their efficiencies were compared. Specifically, the specific activities of dsr365RNP and dsr433RNP were 28.74 and 34.48 (unit/㎍ RNP), respectively. The versatility of this method was also verified using different target genes, uracil phosphoribosyl transferase (upp) gene, of Bifidobacterium bifidum and specific sgRNAs. The assay method was also utilized to determine the impact of high electrical field on Cas9 RNP activity during an efficient electroporation process. Overall, the results demonstrated that the qPCR-based method is an effective tool for measuring the endonuclease activity of Cas9 RNP.

• 유기물자원화
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• 제17권1호
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• pp.96-102
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• 2009

본 연구에서는 혐기성 소화 성능 향상을 위해 초음파 및 알칼리 전처리에 의한 폐활성 슬러지의 가용화 효과를 조사하였다. 초음파 및 알칼리 전처리는 세포벽의 파괴로 인하여 모세관 흡입 시간을 증가시킬 뿐만 아니라 상등액의 용존성 화학적 산소요구량, 단백질 및 탁도 농도를 증가키는 것으로 나타났다. 알칼리와 초음파 전처리를 병행한 슬러지 가용화가 초음파 전처리만을 수행한 경우에 비해 용존성 화학적 산소요구량과 단백질 증가가 높은 것으로 나타났다. 알칼리와 초음파 전처리를 동시에 수행한 경우 폐활성 슬러지의 고형물 농도 증가에 따라 가용화 효율이 감소하는 것으로 조사되었다.

• Journal of Ginseng Research
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• 제48권5호
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• pp.511-519
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• 2024

Background: The cycle of seasonal dormancy of perennating buds is an essential adaptation of perennial plants to unfavorable winter conditions. Plant hormones are key regulators of this critical biological process, which is intricately connected with diverse internal and external factors. Recently, global warming has increased the frequency of aberrant temperature events that negatively affect the dormancy cycle of perennials. Although many studies have been conducted on the perennating organs of Panax ginseng, the molecular aspects of bud dormancy in this species remain largely unknown. Methods: In this study, the molecular physiological responses of three P. ginseng cultivars with different dormancy break phenotypes in the spring were dissected using comparative genome-wide RNA-seq and network analyses. These analyses identified a key role for abscisic acid (ABA) activity in the regulation of bud dormancy. Gene set enrichment analysis revealed that a transcriptional network comprising stress-related hormone responses made a major contribution to the maintenance of dormancy. Results: Increased expression levels of cold response and photosynthesis-related genes were associated with the transition from dormancy to active growth in perennating buds. Finally, the expression patterns of genes encoding ABA transporters, receptors (PYRs/PYLs), PROTEIN PHOSPHATASE 2Cs (PP2Cs), and DELLAs were highly correlated with different dormancy states in three P. ginseng cultivars. Conclusion: This study provides evidence that ABA and stress signaling outputs are intricately connected with a key signaling network to regulate bud dormancy under seasonal conditions in the perennial plant P. ginseng.

• 대순사상논총
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• 제24_2집
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• pp.85-115
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• 2015

The Reordering of the Universe(天地公事) pursuing the Latter world(後天)'s ideal world while denying the Former world(先天) is composed of enormous rituals. The Dosu(度數) is formed through these rituals and the world is transformed according to the Dosu. The people who follow the Sangje(上帝), the subject of this universe reordering and also participate in the Reordering of the Universe are called as the followers. The follower group can be said as a ritual group in the aspect that they participate in the ritual for the Reordering of the Universe. They not only observed the rituals but also actively participated in them, so took certain roles in forming the Dosu. That is, they performed certain, given roles in developing the rituals, or actively proposed their ideas under the Sangje's leadership, and participated in the process realizing the Dosu. Therefore it can be said that they were a group initiatively participating in the process overcoming the Former world's Sangguk(相克) world, and making the latter world's ideal world. It seemed that the followers at that time might have gone through a certain entrance ritual process in order to join in such a follower group. The 『Jeon-Gyeong』 recorded the entrance rituals of a man called as Cha Gyeong Seok among tremendous followers relatively in detail. According to the 『Jeon-Gyeong』, he first had to get a unique test in the entrance process, and the test was proceeded in several stages: the wisdom test reflecting the Sangje's unusual intelligence, the patience test to endure physical, psychological pains for the great ethic, and the test to evaluate the testee's ability to give up past oneself and to follow the Sangje with a new body & mind. In the process separating himself from the past his appearance, Gyeong-Seok, the candidate to enter, faced a liminal situation, and then performed the incorporation rite. In this process, the purification rite going him out of the past relationship of benefits & grudges was performed, and he was given the regulations to comply as a member of the follower group. Through such serial processes, his entrance in the follower group was allowed. What requested to the candidate in this entrance process was the severance from one's past. For that, the candidate was needed to go out of his past life habits, to free from the relationship of benefits and grudges, and to sever him from his past faults. Such severance was the precondition for him to go out of the Former world's negative reality and to break from the Sanggeuk habits. Another thing requesting to the candidate as well as the severance from his past was to establish a new ideal. The new ideal should be matched with the ideal of the Reordering of the Universe whose main ideology was the Haewon-Sangsaeng(解冤相生). Such ideal can be said as the driving force to proceed toward the new ideal world of Latter World. And the candidate was requested to purify his mind and to participate in the Universe Fair Judgement (天地公庭). That can be said as an important condition for the new member directly to participate in the ritual of the Reordering of the Universe dramatically transforming the world. Finally, the candidate was given the regulations for his ascetic practice(修道) as well as his participation in the Reordering, and was requested to comply them. After becoming a member of this follower group, one had to practice his asceticism through the compliance with such regulations and the training of mantras(呪文) in order to accomplish the goal called as Dotong(道通). By fulfilling these requirements, the newly entered member could have the qualification to perform a certain role in the ritual of the Reordering of the Universe, and could accomplish his religious ideal.

• 한국약용작물학회지
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• 제24권4호
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• pp.284-293
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• 2016

Background: Developing new ginseng cultivars is a significant time-consuming process owing to the three years of growth required for ginseng to flower. To shorten the ginseng breeding process, it is necessary to establish rapid progression through each generation. In this study, we examined it was possible to rapidly break ginseng seed dormancy using gibberellic acid ($GA_3$) treatment and alternating temperature. Methods and Results: Seeds were obtained from local variety. Seeds were treated with either $GA_3$ at a concentration of $100mg/{\ell} $, constant temperature ($-2^{\circ}C$ and $2^{\circ}C$), alternating temperature ($2^{\circ}C$ followed by $-2^{\circ}C$, followed by $2^{\circ}C$) or a combination $GA_3$ and temperature treatment. Following experimental treatment, seeds were sown into trays and placed in a greenhouse. Low germination rates were observed in seeds that did not receive $GA_3$ treatment, which were similar following $2^{\circ}C$ and $-2^{\circ}C$ constant temperature treatment. Germination rates increased in proportion to $GA_3$ and more so when combined with alternating temperature treatment. In additon, stem and leaf lengths of the resulting ginseng plants were increased following $GA_3$ treatment, although no synergistic effect was observed with alternating temperature treatment. Conclusions: These results suggest that a combination $GA_3$ and alternating temperature treatment enhances ginseng seed germination, which can contribute to shortening the time required to progress through a single ginseng generation for breeding.

• 한국정보과학회논문지:정보통신
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• 제31권1호
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• pp.81-90
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• 2004

모바일 애드 혹 네트워크 (MANET)는 무선 모바일 노드들이 하부구조(infrastructure)없이 동적으로 네트워크 토폴로지를 구성하는 것을 의미한다. AODV(Ad Hoc On-Demand Distance Vector) 프로토콜은 대표적인 요구기반 라우팅 프로토콜로서, 모바일 노드는 전송할 데이타가 있을 경우에만 라우팅 경로를 찾게 된다. 본 논문에서는 AODV의 지역 경로 복구 기법에 대해 다룬다 라우팅 링크가 단절되면 단절된 링크를 발견한 상위 노드는 목적지 노드까지의 라우팅 경로를 지역 경로 탐색과정을 통해 복구하게 된다. 이 과정에서 AODV 제어 메시지가 단절 경로의 상위노드로부터 목적지 노드까지를 반경으로 하는 지역에 퍼지게 된다. 본 논문에서는 AFLRS(AODV-based Fast Local Repair Scheme)이라고 하는 AODV를 위한 효율적인 지역 경로 복구 기법을 제안한다. AFLRS는 경로 단절이 일어나기 전에 라우팅 노드들이 유지하고 있던 라우팅 정보를 이용하므로 AODV 제어 메시지의 플러딩 범위를 줄이고 경로 복구 시간을 줄일 수 있다. 제안된 AFLRS의 성능평가를 위해 지역경로 기법들을 NS2와 AODV-UU 시뮬레이터를 이용하여 시뮬레이션 하였으며 실험결과에서 AFLRS가 AODV에서의 경로복구기법 보다 빠르게 경로 복구를 할 수 있음을 보인다.