• Journal of Plant Biotechnology
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• 제36권4호
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• pp.407-414
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• 2009

A cDNA clone encoding phytocystatin was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (name as Brcpi1; GenBank accession no.: EF079953) had a total length of 881 bp with an open reading frame of 609 bp, and encoded predicted polypeptide of 203 amino acid (aa) residues including a putative N-terminal signal peptide. Other relevant regions found its sequence included the G and PW conserved aa motifs, and the consensus LARFAV sequence for phytocystatins and the reactive site QVVAG. The BrCPI1 protein shared 95, 94, 81, 80 and 78% identity with other CPI proterins isolated from Brassica oleracea (BoCPI-1), Arabidopsis thaliana (AtCY SB), Glycine max (GmCPI), Oryza sativa (OsCYS-2) and Zea may (ZmCPI) at amino acid level, respectively. Southern blot analysis showed that Brcpi1 was a low copy gene. Expression pattern analysis revealed that Brcpi1 was a tissue-specific expressing gene during reproductive growth and strongly expressed at mature seedling stages. Furthermore, overexpression of Brcpi1 in transgenic Arabidopsis was enhanced tolerance to salt and cold stresses. Meanwhile the juvenile seedling of Brcpi1 transgenic plants was not affected by various concentrations ABA in MS medium. Taken together, the results showed that Brcpi1 functioned as a cysteine protease inhibitor and it exhibited a protective agent against diverse types of abiotic stress, which induced this gene in a tissue- and stress-specific manner.

• 생물환경조절학회지
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• 제31권4호
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• pp.416-422
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• 2022

본 연구는 같은 일적산광량(DLI) 조건에서 각각의 광강도와 광주기 조합이 십자화과 식물 3종의 생육 및 글루코시놀레이트(GSLs) 함량에 미치는 영향을 살펴보고자 수행되었다. 갓(Brassica juncea (L.) Czern.), 적겨자(Brassica juncea L.), 케일(Brassica oleracea L. var. acephala (DC.) Alef.) 종자를 암면 배지에 파종하여 3주간 육묘하였다. 육묘 후 DLI를 10mol·m^-2·d^-1로 설정하여 10h-280, 14h-200, 18h-155, 22h-127µmol·m^-2·s^-1 처리 조합으로 3주간 처리하였다. 14h-200µmol·m^-2·s^-1 처리한 결과, 적갓과 케일은 지상부 생체중/건물중, 엽수, 엽면적이 증가하였지만, 유의적 차이는 관찰되지 않았다. 적갓의 GSLs 함량에서 14h-200µmol·m^-2·s^-1 처리가 10h-280, 18h-155, 22h-127µmol·m^-2·s^-1 처리보다 각각 유의적으로 139.95, 135.87, 154.03% 증가하였으며, 케일의 경우 14h-200µmol·m^-2·s^-1 처리가 다른 처리구보다 각각 132.96, 122.06, 131.78% 유의하게 증가하였다. 적겨자는 18h-155µmol·m^-2·s^-1 처리에서 지상부 생체중/건물중, GSLs 함량이 증가하였으며, 14h-200µmol·m^-2·s^-1 처리에서 엽수가 10h-280, 18h-155, 22h-127µmol·m^-2·s^-1 처리보다 각각 15.62, 12.12, 32.14% 유의적으로 증가하였다. 유사한 십자화과 작물이라도 종에 따라 DLI 반응이 다르기 때문에 최적의 DLI 조건 구명과 최소의 전력 소비량 및 최대 효율을 내기 위한 광질 연구를 병행하여 더세밀한 결과를 도출해야 할 것으로 사료된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권1호
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• pp.97-99
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• 2001

Seven vegetables were planted in green houses to compare their yields and damages by insect pests based on three treatments, green music (GM), ultra sonic (US) and control. The vegetable yields of GM treatment were increased by an average of 17.81% than that of control for two years. There was a remarkable statistic difference in the yield between GM and respective controls such as radish, young radish, cucumber, spinach and Brassica oleracea var. acephala. There was no notable yield difference between US and controls for the semen vegetables within two years. The population densities of the insect pests in GM and US treatments were lower than controls. Only a few insect pests of some vegetables in GM and US treatments injured more seriously than in control.

• Preventive Nutrition and Food Science
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• 제3권4호
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• pp.334-338
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• 1998

Kale(Brassica oleracea var. acephala) is one of Cruciferous vegetables that is closely related to the wild ancestral form of cabbabe. The ethanol extract of kale which contains the active compoundsss under Salmonella assay system was fractionated with chloroform to collect the nonpolar solvent soluble compounds, and then further fractionation was carried out by silica gel column chromatography. Among kale extracts separated by silical gel column chromatography, the fractions of 4, 5 and 6 exhibited strong antimutagenic activities. The major active compounds from the fraction were identified as chlorophyll derivatives by the analysis with HPLC-fritp-MS. The molecular weights of each chlorophyll derivatives in the sample were acquired from the peaks of positive ion atomosphere pressure chemical ionization (APCI) mas spectrometry.

• Mycobiology
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• 제45권2호
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• pp.110-113
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• 2017

Severe root rot was observed in fields of cabbages (Brassica oleracea L.) in 2015 in China. Cardinal symptoms of this disease included root rot and wilting leaves. A fungus was isolated from diseased tissues consistently. Based on the morphological features and molecular analysis of the ITS-5.8S rDNA and D1/D2 domain of the 28S rRNA gene, it was identified as Plectosphaerella cucumerina. This is the first report of P. cucumerina causing cabbage root rot in China and the world.

• Preventive Nutrition and Food Science
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• 제16권2호
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• pp.189-193
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• 2011

Kohlrabi (Brassica oleracea var. gongylodes L.) slices were dehydrated with maltodextrin (MD) at concentrations of 20, 30, and 40% (w/w), and the dried samples were compared with the freeze-dried and hot-air dried samples regarding various physicochemical qualities. The MD-treated samples had better results than those of freeze-dried or hot-air dried samples in terms of rehydration ratio and color. The total phenolic content of the MD-treated sample was similar to that of the freeze-dried and higher than that of hot-air dried sample. The ascorbic acid content of the MD-treated samples was also higher than that of the hot-air dried one. These results suggest that kohlrabi can be dehydrated with MD instead of hot air.

• 한국생물환경조절학회:학술대회논문집
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• 한국생물환경조절학회 2003년도 춘계 학술대회 논문집
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• pp.82-85
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• 2003

녹색꽃양배추는 십자화과에 속하는 양배추의 변종으로 꽃이 피지 않은 화뢰를 수확대상으로 한다. 녹색꽃양배추는 녹황색 채소 가운데 영양가가 많은 채소로, 여느 채소보다 비타민 AㆍC의 함량이 뛰어나며 특히 비타민 C는 과일을 능가할 정도로 양상추의 27배, 그린아스파라거스의 13배나 많이 함유하고 있다. 또한 녹색꽃양배추에 함유된 sulforaphane은 항발암 및 해독효소 효과가 큰 것으로 알려져 있으며(Aspry, 1983; Jhang, 1992; Kim, 1997; Howard, 1997), 외국에서는 일찍부터 중요한 채소의 하나로 이용되고 있다. (중략)

• 동아시아식생활학회지
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• 제20권2호
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• pp.291-298
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• 2010

The influence of cabbage powder on sensory acceptance and physical functions of Jeungpyun, were assessed. As fermentation continued, pH dropped and evolved carbon dioxide significantly increased. Moisture content of cabbage powder-amended Jeungpyun ranged from 51.1~55.5%, and were lower than those of control samples. Jeungpyun prepared with 8~10% cabbage powder displayed significantly lower specific volumes. As the level of cabbage powder increased, the L-value was reduced, while the a- and b-values increased. Hardness was not significantly different among the control, 2%, 4% and 6% samples. Chewiness tended to increased in proportion to the level of cabbage powder. Sensory scores were lowest for Jeungpyun prepared with 8~10% cabbage powder. The results indicate that addition of 4~6% cabbage powder to Jeungpyun produces the optimal product.

• Journal of Plant Biology
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• 제17권1호
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• pp.3-8
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• 1974

The experiments were conducted to examine the effects of sulfur dioxide on catalase activity in germinating seeds under dark conditions and in green plants under light conditions. Three kinds of plants, bean (Glycine max M.), cabbage (Brassica oleracea L. Chungbang No.1) and rice (Oryza sativa L. Suwon No.213-1) were used in this study. Both thegerminating seeds and the leaves were treated with various concentrations of sulfur dioxide for both one green plants with two and twelve hours. In the results of the measurement of catalase activity it was found that plant injuries by sulfur dioxide for the period of photosynthesis were higher than those for the period of respiration. In the control, apparent catalase activity under the light condition of photosynthesis showed a considerable decrease in comparison with catalase activity under the dark condition of respiration. This tendency also appeared markedly in the gas treatment. In the gas treatment for twelve hours, the decrease of catalase activity was higher than that of one hour in both photosynthesis and respiraton. It was thought that sulfur dioxide was an inhibitor of catalase activity in higher concentration of the gas.

• 한국식품조리과학회지
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• 제31권5호
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• pp.614-622
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• 2015

This study was conducted to investigate the quality and antioxidant properties of white breads enhanced with broccoli powder (BP) (0%, 2.5%, 5.0%, 7.5% and 10.0%). L-value significantly decreased with increasing levels of BP, but a- and b-values increased (p<0.05). Baking loss rate, bread volume, and specific volume were reduced with the addition of BP, whereas the pH of the dough and bread weight increased. The hardness, gumminess and brittleness of breads with BP were higher than those of control breads (p<0.05). Upon sensory evaluation, the 2.5% and 5.0% BP breads showed no significant differences in color, flavor, taste, texture, or overall acceptability compared with controls (p<0.05). The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of bread significantly increased with increasing BP content (p<0.05). Accordingly, bread quality can be improved by the substitution of 5% BP in place of flour.