• Title/Summary/Keyword: Branch Lines

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The Effect of Overexpression of Rat Clusterin in L929 Fibroblasts

  • PARK , JUNG-HYUN;JU, SUNG-KYU;PARK, JEE-SUN;PARK, YOO-KYOUNG;KANG, MYUNG-HEE;YOU, KWAN-HEE
    • Journal of Microbiology and Biotechnology
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    • v.14 no.6
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    • pp.1333-1337
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    • 2004
  • Oxidants such as hydrogen peroxide are powerful inducers of cell damage, ageing, and apoptosis. Since clusterin, a 75-80 kDa mammalian glycoprotein, is frequently found to be inducible in apoptotic cells and tissues, this study inquired into whether this would be a protective mechanism against further cell death. The aim was to find out whether overexpression of clusterin could protect cells from oxidant­induced stress and apoptosis. To clarify this issue, we generated and analyzed stable cell lines expressing fusion proteins of a rat clusterin with an enhanced green fluorescent protein (EGFP). When treated with varying concentrations of hydrogen peroxides, clusterin transfectants indeed showed increased resistance to apoptosis and exhibited a much higher survival rate than mock-transfected cells. On the other hand, neither intracellular re-distribution nor local concentration of clusterin-EGFP was observed, which leaves the question open about its anti-apoptotic mechanism. In conclusion, the overexpression of clusterin provides a means for protecting cells against oxidative stress and subsequent cell death.

The Effective Parallel Processing Method for an Enhanced Digital Image of Skeleton Line (향상된 영상 골격화를 위한 효과적인 병렬 처리 방법)

  • 신충호;오무송
    • Journal of Korea Multimedia Society
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    • v.7 no.4
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    • pp.459-466
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    • 2004
  • In this paper, an effective skeleton method is proposed in order to obtain an enhanced digital image of skeleton line. The binary image using the threshold values is applied in the preprocessing stage and then the modified parallel processing method is applied to obtain the improved image of skeleton line. The existing skeleton methods are Rutovitz, Steiabelli and other five skeleton methods. In the digital process of skeleton line, the major problem caused by these methods is elongated lines and noise branches of the processed image. In this study, however, such noises are deleted first by the modified parallel processing step of the proposed method. Then a pixel is compared to its eight neighbor pixels. if its neighbor pixels are in one of the eight conditions, the central pixel is deleted. As a result, the quality of the skeleton is better then those produced by the existing skeleton methods.

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Characterization of a Substance from Photobacterium damsela subsp. piscicida that Non-specifically Binds to Streptavidin

  • Jung Tae Sung;Kim D. Thompson;Adams Aelexandra;Oh Myung Joo
    • Fisheries and Aquatic Sciences
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    • v.3 no.1
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    • pp.52-63
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    • 2000
  • Non-specific reaction has been a problem in doing, especially, research and diagnosis for infectious agents. Avidin-biotin-peroxidase complex (ABC) techniques has widely been used to amplify a reaction. Photobacterium damse1a subsp. piscicdia (formerly Pasteurella piscicida) exhibited a capacity to bind with streptavidin non-specifically. The band, estimated 26 K Da in Western blotted paper, was blocked with biotin but incompletely. In an attempt to explore an involvement of the non-specific substance in attaching piscine cells, cell attachment test performed using anti- Ph. d. subsp piscicida sera raised mouse and rabbit exhibited slightly blocking effects for Mediterranean (1736) and significantly for Japanese (Sp 92144) isolate. Biotin decreased the attachment ability significantly for Sp92144 but it was not effective to 1736. Both isolates showed greatly enhanced attachment ability with poly-L-lysin. The non-specific binding substance was contained in bacterial extracellular products (ECPs). The substance was able to purified with 2-imminobiotin affinity column, the purified substance appeared to have 4 bands in silver staining, and had a carbohydrate branch. This purified substance showed cytotoxic effects selectively between 5 piscine cell lines. Moreover, it stimulated rainbow trout macrophage in terms of reduction of cytochrome cas well as yeast phagocytosis, significantly.

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Remote Acoustic Sensing Methods for Studies in Oceanology

  • Akulichev, Victor A.;Bezotvetnykh, Vladimir V.;Burenin, Alexander V.;Voytenko, Evgeny A.;Kamenev, Sergey I.;Morgunov, Yury N.;Polovinka, Yury A.;Strobykin, Dmitry S.
    • Ocean Science Journal
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    • v.41 no.2
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    • pp.105-111
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    • 2006
  • In this paper, the lines of investigation on a problem of the development of remote acoustic sensing methods in oceanology are formulated. This paper summarizes the results of investigations into the possibilities for monitoring temperature and flow fields in shallow seas. In the discussed experiments, the instrumentation being constituents of the complex for long-duration remote monitoring of marine medium climatic variability and that of the acoustic tomography of shallow sea dynamic processes is used. The acoustic instruments were located on the POI FEB RAS acousto-hydrophysical polygon (Pacific Oceanological Institute, Far Eastern Branch of the Russian Academy of Sciences) near the Gamov Peninsula. Acoustic receiving and transmitting systems operating with multiplex phase-manipulated signals (of M-codes) at frequency range 250-2500 Hz form the basis for this complex.

Design of Planar Power Divider Combiner for K-Band and Improvement of Impedence Matching Condition (K-밴드 평면형 Power Divider / Combiner와 정합특성에 관한 연구)

  • 나극환;홍의석;강준길;김춘길
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.14 no.6
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    • pp.579-589
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    • 1989
  • In this paper, planar power dividers/combiners for millimeter waves K-band or bands which can be printed on the substrates of hybrid or monolithic IC by surface mounting are designed and studied. Power dividers/combiners, and the conductor loss of microstrip lines in particualr the existing Wilkinson power dividers/combiners is modified ad amployes by ist equivalent circuit. Microwave CAD program SUPEROMPACT is employed for the Wilkinson power combiner which is modified and analyzed to reduce the high frequency coupling between the branches of the combiner, and the method to diminish the sensitivity of the input reflection of $2^n$-way power dividers/combiners is studied employing the commerical microwave CAD program package SUPERCOMPACT.

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Hydraulic Adequacy of Connection Pipes in Water Supply Systems for Contingencies (비상시 용수공급을 위한 상수도 연계관로의 수리적 적정성 평가)

  • Han, Wanseob;Jung, Kwansoo;Kim, Juhwan
    • Journal of Korean Society of Water and Wastewater
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    • v.27 no.6
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    • pp.679-687
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    • 2013
  • Although stable and safe drinking water supply to the customers is a basic function of multi-regional water supply systems in Korea, most systems have their vulnerabilities in emergency time due to the branch-type. Application of connections from the other water supply system can provide a solutions for these tentative problems. This paper describes reduction planning of water supply accidents that can minimize a service interruption to customers in multi-regional water supply system by connecting pipe lines between local water supply systems in Mokpo city areas. The result of this study shows that Juam dam multi-regional water supply systems can cover all of the water shortage in southern parts of Jeonnam multi-regional water supply systems by transmitting water through connected pipes between local networks. This can be effective to supply water interactively in various contingencies, when a pipe line accident occurs in southern area of Jeonnam multi-regional water supply systems. On the contrary, southern area of Jeonnam multi-regional water supply systems can cover 99.5 %($62,500m^3/day$) of the water shortage of Juam dam multi-regional water supply systems when service interruptions caused by various pipe accidents occur in the system.

Simultaneous Observations of SiO and $H_2O$ Masers toward Known Stellar $H_2O$-only Maser Sources

  • Kim, Jae-Heon;Cho, Se-Hyung;Kim, Sang-Joon
    • The Bulletin of The Korean Astronomical Society
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    • v.36 no.2
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    • pp.145.2-145.2
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    • 2011
  • We present the results of simultaneous observations of SiO v = 1, 2, J = 1-0, $^{29}SiO$ v = 0, J = 1-0, and $H_2O$ $6_{16}-5_{23}$ maser lines toward 152 known $H_2O$-only maser sources (the sources which are previously detected only in the 22 GHz $H_2O$ maser emission) using Yonsei and Tamna 21-m radio telescopes of the Korean VLBI Network from 2009 June to 2011 January. Both SiO and $H_2O$ maser emission were detected from 62 sources giving a detection rate of 40.8 %. SiO-only maser emission was detected from 27 sources, while $H_2O$-only maser was detected from 22 sources. We have identified 19 new detections of SiO maser emission for previous non-detection sources and 51 new detections of SiO maser for previously not observed sources. Characteristics of all observed sources in the IRAS two-color diagram is investigated including their evolutionary sequence and mutual relations between SiO and $H_2O$ maser emission. These observational results will be useful for statistical study of asymptotic giant branch (AGB) stars and future VLBI observation.

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Transmission System Expansion Planning by Nodal Delivery Marginal Rate Criterion -II (모선수송전달능력(母線輸送傳達能力) 신뢰도 기준에 의한 송전계통(送電系統)의 광역설계(擴充計劃) -II)

  • Park, Jeong-Je;Shi, Bo;Jeong, Sang-Hun;Choi, Jae-Seok;Mount, Timothy;Thomas, Robert
    • Proceedings of the KIEE Conference
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    • 2007.07a
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    • pp.574-575
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    • 2007
  • This paper proposes a method for choosing the best transmission system expansion plan using nodal/bus delivery marginal rate criterion ($BMR_k$) defined newly in this paper. The objective method minimizes a total cost which is an investment budget for constructing new transmission lines subject to the $BMR_k$ which means a nodal deterministic reliability level requirement at specified load point. The proposed method models the transmission system expansion problem as an integer programming problem. It solves for the optimal strategy using a branch and bound method that utilizes a network flow approach and the maximum flow-minimum cut set theorem. Test results on an existing 21-bus system are included in the paper. It demonstrated the suitability of the proposed method for solving the transmission system expansion planning problem in competitive electricity market environment.

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Considering on the Ground Reflection Effect on the Electromagnetic Fields due to Lightning Channel

  • Izadi, Mahdi;Ab Kadir, Mohd Zainal Abidin;Hajikhani, Maryam
    • Journal of Electrical Engineering and Technology
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    • v.8 no.4
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    • pp.824-831
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    • 2013
  • Lightning electromagnetic fields are important issues for the evaluation of lightning induced overvoltage on power lines and for setting the appropriate protection level for power networks. Such electromagnetic fields are strongly dependent on lightning return stroke currents at different heights along the lightning channel. On the other hand, the ground reflection factor due to the difference between the return stroke channel impedance and the equivalent ground impedance at channel base can have an effect on the shape of the return stroke currents by entering additional reflected currents into the channel. In this paper, the effect of the ground reflection factor on the return stroke currents at different heights along a channel and the electromagnetic fields associated with the lightning channel at close distances are considered. Moreover, the behavior of the electromagnetic fields versus the reflection factor changes and the radial distance changes are considered and the results are discussed accordingly. The results illustrate that the reflection factor has a direct relationship with the values of the electromagnetic fields while this is usually ignored in earlier studies.

Consideration of Ambiguties on Transmission System Expansion Planning using Fuzzy Set Theory (애매성을 고려한 퍼지이론을 이용한 송전망확충계획에 관한 연구)

  • Tran, T.;Kim, H.;Choi, J.
    • Proceedings of the KIEE Conference
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    • 2004.11b
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    • pp.261-265
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    • 2004
  • This paper proposes a fuzzy dual method for analyzing long-term transmission system expansion planning problem considering ambiguities of the power system using fuzzy lineal programming. Transmission expansion planning problem can be formulated integer programming or linear programming with minimization total cost subject to reliability (load balance). A long-term expansion planning problem of a grid is very complex, which have uncertainties fur budget, reliability criteria and construction time. Too much computation time is asked for actual system. Fuzzy set theory can be used efficiently in order to consider ambiguity of the investment budget (economics) for constructing the new transmission lines and the delivery marginal rate (reliability criteria) of the system in this paper. This paper presents formulation of fuzzy dual method as first step for developing a fuzzy Ford-Fulkerson algorithm in future and demonstrates sample study. In application study, firstly, a case study using fuzzy integer programming with branch and bound method is presented for practical system. Secondly, the other case study with crisp Ford Fulkerson is presented.

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