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The Effect of Overexpression of Rat Clusterin in L929 Fibroblasts

PARK , JUNG-HYUN (Experimental Immunology Branch, National Cancer Institute)
JU, SUNG-KYU (School of Bioscience and Biotechnology, Chungnam National University)
PARK, JEE-SUN (School of Bioscience and Biotechnology, Chungnam National University)
PARK, YOO-KYOUNG (Department of Food and Nutrition, Hannam University)
KANG, MYUNG-HEE (Department of Food and Nutrition, Hannam University)
YOU, KWAN-HEE (School of Bioscience and Biotechnology, Chungnam National University)

Publication Information

Journal of Microbiology and Biotechnology / v.14, no.6, 2004 , pp. 1333-1337 More about this Journal

Abstract

Oxidants such as hydrogen peroxide are powerful inducers of cell damage, ageing, and apoptosis. Since clusterin, a 75-80 kDa mammalian glycoprotein, is frequently found to be inducible in apoptotic cells and tissues, this study inquired into whether this would be a protective mechanism against further cell death. The aim was to find out whether overexpression of clusterin could protect cells from oxidantinduced stress and apoptosis. To clarify this issue, we generated and analyzed stable cell lines expressing fusion proteins of a rat clusterin with an enhanced green fluorescent protein (EGFP). When treated with varying concentrations of hydrogen peroxides, clusterin transfectants indeed showed increased resistance to apoptosis and exhibited a much higher survival rate than mock-transfected cells. On the other hand, neither intracellular re-distribution nor local concentration of clusterin-EGFP was observed, which leaves the question open about its anti-apoptotic mechanism. In conclusion, the overexpression of clusterin provides a means for protecting cells against oxidative stress and subsequent cell death.

Keywords

Apoptosis; clusterin; green fluorescent protein; oxidants;

Citations & Related Records

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