• 대한인간공학회지
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• 제32권5호
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• pp.421-427
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• 2013

Objective: This paper is to analyze the impact of musical training to the fast ${\alpha}$ wave activation of the EEG. Background: EEG is neurological research method that can observe the brain function in real time. EEG can be used to determine the nervousness and relaxedness of a person who receives stimuli in a structured environment. Therefore, it is possible to interpret the functional state of human brain by the analysis of EEG. Method: The brain activities of two groups of university students in the point of RFA(Relative Fast Alpha) caused by different music are analyzed in this paper. One is the group of music majors and the other is the group of non-majors. Results: Music major and non-major groups show meaningful differences in RFA during exposed to classic and metal music. Conclusion: Learning experience on music affects RFA increment of music majors. Application: The result of this study will be used as basic data to evaluate the learning effects of students who want to study music.

• Animal cells and systems
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• 제2권2호
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• pp.223-227
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• 1998

We isolated two soluble forms of glutamate dehydrogenase isoproteins, GDH I and GDH II, from bovine brain. The regulation of GDH I and GDH II by phosphorylation and dephosphorylation has been examined in various conditions. There were dose- and time- dependent activation of the GDH isoproteins when phosphorylated by cAMP-dependent protein kinase. The phosphorylated GDH had 1.1 mol of covalently bound phosphate/mol of subunit and a 2-fold increased specific activity. The phosphorylated amino acid was identified as serine. When treated with alkaline phosphatase, the activities of the phosphorylated GDH isoproteins were reduced in dose and time dependent manner and returned to those of unphosphorylated enzymes. There were no significant differences between GDH I and GDH II in their sensitivities to the action of phosphorylation and dephosphorylation demonstrating that the microenvironmental structures of the phosphorylation site in GDH isoproteins are similar to each other, These results results suggest that the inter-conversion between less active form of brain GDH isoproteins and more active form is regulated by phosphorylation through cAMP-dependent protein kineses.

• 전기전자학회논문지
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• 제22권4호
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• pp.1195-1201
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• 2018

본 논문은 인간의 신체활동에 있어서 뇌의 신호가 연결된 근육으로 정보를 제공하고 받아오는 과정에서 유의미한 결과를 나타내는지에 관한 관계를 해석한다. 사람의 의식적 활동은 활동에 필요한 근육의 동작을 위하여 뇌로부터 생성된 전기신호의 전달에 의해 가능해 진다. 근육의 활성정보를 가지고 있는 근전도 신호는 근육활성화의 결과를 전기적인 신호로 출력하는데, 이 출력은 보통 근육의 수축과 이완에 따른 근육활성 정보를 출력한다. 본 연구에서는 이런 뇌전도와 근전도를 실시간으로 추출하여 데이터를 획득하고, 데이터 분석을 통해 눈으로 쉽게 확인하기 어려운 두 신호간의 관계를 분석하는데 목적이 있다.

• BMB Reports
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• 제50권11호
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• pp.590-595
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• 2017

High-mobility group box-1 (HMGB-1) is expressed in almost all cells, and its dysregulated expression correlates with inflammatory diseases, ischemia, and cancer. Some of these conditions accompany HMGB-1-mediated abnormal angiogenesis. Thus far, the mechanism of HMGB-1-induced angiogenesis remains largely unknown. In this study, we performed time-dependent DNA microarray analysis of endothelial cells (ECs) after HMGB-1 or VEGF treatment. The pathway analysis of each gene set upregulated by HMGB-1 or VEGF showed that most HMGB-1-induced angiogenic pathways were also activated by VEGF, although the activation time and gene sets belonging to the pathways differed. In addition, HMGB-1 upregulated some VEGFR signaling-related angiogenic factors including EGR1 and, importantly, novel angiogenic factors, such as ABL2, CEACAM1, KIT, and VIPR1, which are reported to independently promote angiogenesis under physiological and pathological conditions. Our findings suggest that HMGB-1 independently induces angiogenesis by activating HMGB-1-specific angiogenic factors and also functions as an accelerator for VEGF-mediated conventional angiogenesis.

• 재활치료과학
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• 제7권2호
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• pp.9-16
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• 2018

목적 : 본 연구의 목적은 뇌손상 환자의 신경학적 변화 측정도구인 뇌전도의 측정도구 및 방법, 평가와 분석방법을 알아봄으로써 임상영역에서 뇌전도 측정 시 기초자료를 제시하는 것에 있다. 연구방법 : 전자 데이터 베이스인 Pubmed, Science Direct를 사용하였으며, 주요검색 용어로 'Electroencephalography', 'stroke', 'intervention OR training'을 사용하였다. 결과 : 뇌전도는 두뇌-컴퓨터 인터페이스(Brain-computer interface)를 이용하여 재활의 효과를 뇌의 활성화 상태 변화로 측정할 수 있는 도구로 기능적 뇌 재조직화 매커니즘을 확인할 수 있는 것으로 나타났다. 뇌전도 측정도구의 경우 다양한 채널, 전극의 형태 및 전극 부착 부위로 구성되어 있으며, 결과해석에 사용되는 주파수 또한 다양하게 나타났다. 결론 : 뇌전도는 중재에 대한 효과성을 신경학적으로 확인할 수 있을 뿐만 아니라 효율적인 작업치료를 위한 중재전략을 마련하는데 사용될 수 있을 것으로 사료된다.

• BMB Reports
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• 제52권3호
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• pp.190-195
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• 2019

Acetaminophen (APAP) overdose can cause hepatotoxicity by inducing mitochondrial damage and subsequent necrosis in hepatocytes. Sirtuin2 (Sirt2) is an $NAD^+$-dependent deacetylase that regulates several biological processes, including hepatic gluconeogenesis, as well as inflammatory pathways. We show that APAP decreases the expression of Sirt2. Moreover, the ablation of Sirt2 attenuates APAP-induced liver injuries, such as oxidative stress and mitochondrial damage in hepatocytes. We found that Sirt2 deficiency alleviates the APAP-mediated endoplasmic reticulum (ER) stress and phosphorylation of the p70 ribosomal S6 kinase 1 (S6K1). Moreover, Sirt2 interacts with and deacetylates S6K1, followed by S6K1 phosphorylation induction. This study elucidates the molecular mechanisms underlying the protective role of Sirt2 inactivation in APAP-induced liver injuries.

• Journal of Ginseng Research
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• 제46권4호
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• pp.572-584
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• 2022

Background: Huntington's disease (HD) is a neurodegenerative disorder caused by the expansion of trinucleotide CAG repeat in the Huntingtin (Htt) gene. The major pathogenic pathways underlying HD involve the impairment of cellular energy homeostasis and DNA damage in the brain. The protein kinase ataxia-telangiectasia mutated (ATM) is an important regulator of the DNA damage response. ATM is involved in the phosphorylation of AMP-activated protein kinase (AMPK), suggesting that AMPK plays a critical role in response to DNA damage. Herein, we demonstrated that expression of polyQ-expanded mutant Htt (mHtt) enhanced the phosphorylation of ATM. Ginsenoside is the main and most effective component of Panax ginseng. However, the protective effect of a ginsenoside (compound K, CK) in HD remains unclear and warrants further investigation. Methods: This study used the R6/2 transgenic mouse model of HD and performed behavioral tests, survival rate, histological analyses, and immunoblot assays. Results: The systematic administration of CK into R6/2 mice suppressed the activation of ATM/AMPK and reduced neuronal toxicity and mHTT aggregation. Most importantly, CK increased neuronal density and lifespan and improved motor dysfunction in R6/2 mice. Conversely, CK enhanced the expression of Bcl2 protected striatal cells from the toxicity induced by the overactivation of mHtt and AMPK. Conclusions: Thus, the oral administration of CK reduced the disease progression and markedly enhanced lifespan in the transgenic mouse model (R6/2) of HD.

• BMB Reports
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• 제55권10호
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• pp.512-517
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• 2022

Traumatic brain injury (TBI) is brain damage which is caused by the impact of external mechanical forces. TBI can lead to the temporary or permanent impairment of physical and cognitive abilities, resulting in abnormal behavior. We recently observed that a single session of early exercise in animals with TBI improved their behavioral performance in the absence of other cognitive abnormalities. In the present study, we investigated the therapeutic effects of continuous exercise during the early stages of TBI in rats. We found that continuous low-intensity exercise in early-stage improves the locomotion recovery in the TBI of animal models; however, it does not significantly enhance short-term memory capabilities. Moreover, continuous early exercise not only reduces the protein expression of cerebral damage-related markers, such as Glial Fibrillary Acid Protein (GFAP), Neuron-Specific Enolase (NSE), S100β, Protein Gene Products 9.5 (PGP9.5), and Heat Shock Protein 70 (HSP70), but it also decreases the expression of apoptosis-related protein BAX and cleaved caspase 3. Furthermore, exercise training in animals with TBI decreases the microglia activation and the expression of inflammatory cytokines in the serum, such as CCL20, IL-13, IL-1α, and IL-1β. These findings thus demonstrate that early exercise therapy for TBI may be an effective strategy in improving physiological function, and that serum protein levels are useful biomarkers for the predicition of the effectiveness of early exercise therapy.

• Journal of Acupuncture Research
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• 제21권2호
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• pp.57-71
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• 2004

Objective : Physiological evidence regarding acupuncture's effect in human patients is not yet well established, despite considerable evidence for its therapeutic efficacy. Besides target or disease specificity of acupuncture, acupuncture analgesia (AA) appears to be another large subclass that poses many questions, such as whether there is point specificity with respect to which acupoint is most effective for a particular condition. Methods : We observed brain activation with functional magnetic resonance imaging (fMRI) using a set of stimuli that consist of pain, pain following Meridian acupuncture, and pain following Sham acupuncture. Results : Among the new observations, the most interesting fact is that data sets of both Meridian acupuncture and Sham acupuncture show decreased activation of the same brain areas related to the pain processing signals. Present functional MRI study demonstrate two important biological observations that could elucidate AA mechanism in human participants: the effects of acupuncture occur through mediation of the higher brain areas. Sham acupuncture stimulation appears to be almost as effective as traditional Meridian acupoint stimulation, suggesting that acupuncture is not entirely point specific. Decreased activation in the limbic paleo cortical areas appears to be the probable neurological manifestation of AA and strongly implies that acupuncture stimulation inhibits the transmission of ascending pain signals to the higher cortical areas by the previously known descending pain inhibitory circuit. Conclusion : We, therefore, a hypothesized that this pain inhibitory circuit is initiated and mediated via the broad sense Hypothalamus Pituitary Adrenal (BS HPA) axis in conjunction to the "sensory stimulation."

• 한국산학기술학회논문지
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• 제19권5호
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• pp.312-322
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• 2018

본 연구는 뇌 활성화 중재프로그램을 경증치매노인에게 적용하여 경증치매노인의 인지기능, 행동심리적 증상, 우울 및 일상생활수행능력에 미치는 효과를 파악하는데 목적을 두고 수행 하였다. 본 연구의 연구 대상자는 실험군 20명과 대조군 20명으로 총 40명이었다. 본 연구의 자료 수집은 서울시 노원구에 소재한 치매지원센터에서 2016년 11월 1일부터 2017년 3월 31일까지 자료가 수집 되었다. 수집된 자료의 분석방법은 SPSS 23.0K을 이용하여 기술통계, Chi-square test, independent t-test, Two-way Repeated Measures ANOVA, ANCOVA로 분석 하였다. 본 연구에서 경증치매노인을 대상으로 뇌 활성화 중재프로그램을 8주간의 강의 및 프로그램을 시행한 결과 대상자 경증치매노인의 인지기능이 유의하게 향상되었고, 우울감이 유의하게 감소하였다. 본 연구 결과를 토대로 치매노인에게 적용한 뇌 활성화 중재프로그램으로 인지기능의 향상으로 일상생활에도 도움을 줄 수 있고, 치매노인의 우울을 완화시키는 것으로 나타나 차후에 지역사회 프로그램에 활용되도록 하는 기초자료로 지역사회에서 연계하는 프로그램으로 도움이 될 것으로 사료된다.