• Biomolecules & Therapeutics
• /
• v.27 no.3
• /
• pp.290-301
• /
• 2019

Paeonol has neuroprotective function, which could be useful for improving central nervous system disorder. The purpose of this study was to characterize the functional mechanism involved in brain transport of paeonol through blood-brain barrier (BBB). Brain transport of paeonol was characterized by internal carotid artery perfusion (ICAP), carotid artery single injection technique (brain uptake index, BUI) and intravenous (IV) injection technique in vivo. The transport mechanism of paeonol was examined using conditionally immortalized rat brain capillary endothelial cell line (TR-BBB) as an in vitro model of BBB. Brain volume of distribution (VD) of [$^3H$]paeonol in rat brain was about 6-fold higher than that of [$^{14}C$]sucrose, the vascular space marker of BBB. The uptake of [$^3H$]paeonol was concentration-dependent. Brain volume of distribution of paeonol and BUI as in vivo and inhibition of analog as in vitro studies presented significant reduction effect in the presence of unlabeled lipophilic compounds such as paeonol, imperatorin, diphenhydramine, pyrilamine, tramadol and ALC during the uptake of [$^3H$]paeonol. In addition, the uptake significantly decreased and increased at the acidic and alkaline pH in both extracellular and intracellular study, respectively. In the presence of metabolic inhibitor, the uptake reduced significantly but not affected by sodium free or membrane potential disruption. Similarly, paeonol uptake was not affected on OCTN2 or rPMAT siRNA transfection BBB cells. Interestingly. Paeonol is actively transported from the blood to brain across the BBB by a carrier mediated transporter system.

• Proceedings of the KOSOMBE Conference
• /
• v.1997 no.11
• /
• pp.213-217
• /
• 1997

In this study, three-dimensional inite element model was developed and analyzed or DAI using ABAQUS. To verify the developed FE model, simulated results were compared to experimental results of human cadaver by Nahum et. al. (1977). The effect of acceleration pattern and accelerating duration time or DAI was analyzed by means of maximum shear stress and pressure distribution. DAI was favored or angular acceleration rather than linear acceleration, and occured in brain stem, pons and midbrain easily as accelerating duration time was increased.

• Proceedings of the Korean Institute of Intelligent Systems Conference
• /
• 2006.05a
• /
• pp.45-48
• /
• 2006

The system that can generate biological brain information processing mechanism more precisely may have several abilities such as exact cognition, situation decision, learning and inference, and output decision. In this paper, to implement high level information processing and thinking ability in a complex system, the information processing layer based on the biological brain is introduced. The biological brain information processing mechanism, which is analyzed in this paper, provides fundamental information about intelligent engineering system, and the design of the layer that can mimic the functions of a brain through engineering definitions can efficiently introduce an intelligent information processing method having a consistent flow in various engineering systems. The applications proposed in this paper are expected to take several roles as a unified model that generates information process in various areas, such as engineering and medical field, with a dream of implementing humanoid artificial intelligent system.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.16 no.11
• /
• pp.3479-3492
• /
• 2022

Longitudinal quantification of brain changes due to development, aging or disease plays an important role in the filed of personalized-medicine applications. However, due to the temporal variability in shape and different imaging equipment and parameters, estimating anatomical changes in longitudinal studies is significantly challenging. In this paper, a longitudinal Magnetic Resonance(MR) brain image segmentation algorithm proposed by combining intensity information and anisotropic smoothness term which contain a spatial smoothness constraint and longitudinal consistent constraint into a variational framework. The minimization of the proposed energy functional is strictly and effectively derived from a fast optimization algorithm. A large number of experimental results show that the proposed method can guarantee segmentation accuracy and longitudinal consistency in both simulated and real longitudinal MR brain images for analysis of anatomical changes over time.

• Journal of Korean Neurosurgical Society
• /
• v.58 no.1
• /
• pp.22-29
• /
• 2015

Objective : Perinatal hypoxic-ischemic encephalopathy (HIE) and prolonged febrile seizures (pFS) are common neurologic problems that occur during childhood. However, there is insufficient evidence from experimental studies to conclude that pFS directly induces hippocampal injury. We studied cognitive function and histological changes in a rat model and investigated which among pFS, HIE, or a dual pathologic effect is most detrimental to the health of children. Methods : A rat model of HIE at postnatal day (PD) 7 and a pFS model at PD10 were used. Behavioral and cognitive functions were investigated by means of weekly open field tests from postnatal week (PW) 3 to PW7, and by daily testing with the Morris water maze test at PW8. Pathological changes in the hippocampus were observed in the control, pFS, HIE, and HIE+pFS groups at PW9. Results : The HIE priming group showed a seizure-prone state. The Morris water maze test revealed a decline in cognitive function in the HIE and HIE+pFS groups compared with the pFS and control groups. Additionally, the HIE and HIE+pFS groups showed significant hippocampal neuronal damage, astrogliosis, and volume loss, after maturation. The pFS alone induced minimal hippocampal neuronal damage without astrogliosis or volume loss. Conclusion : Our findings suggest that pFS alone causes no considerable memory or behavioral impairment, or cellular change. In contrast, HIE results in lasting memory impairment and neuronal damage, gliosis, and tissue loss. These findings may contribute to the understanding of the developing brain concerning conditions caused by HIE or pFS.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.21 no.5
• /
• pp.240-248
• /
• 2020

Over the past 20 years, brain science has developed rapidly thanks to new technologies such as functional magnetic resonance imaging (fMRI), leading to more accurate knowledge of human nature and behavioral changes. This knowledge is also actively applied in the field of management. This research aimed to gain insights into how neuroscience can be incorporated into management through the case of Midas IT Co. This construction software company has a separate organization with the purpose of studying brain science, and it makes and implements human resource management policies based on brain science. The founder Lee Hyung-woo has a humanist management philosophy, and the company's brain science research supports that philosophy. The case study method was adopted as the research method, and procedures such as interviews and direct observation, participation observation, and document information were carried out. The company's human resource management system can be explained by a brain science model called "SCARF", which combines various neuroscience discoveries. As this model suggests, the company has improved the trust and satisfaction of its members by reducing threat of status and by increasing certainty, autonomy, relationship, and fairness in the workplace, resulting in the creation of a platform for creativity, integrity, and high performance.

• Journal of the Institute of Electronics Engineers of Korea SC
• /
• v.47 no.5
• /
• pp.18-23
• /
• 2010

In this paper, we propose a computationally efficient method detecting the P300 wave for brain-machine interface. Electrophysiological researches have shown that the P300 wave's potential is decreased when human intention matches visual stimulation. Motivated by this fact, we can infer human intention for brain-machine interface by detecting the P300 wave's potential decrease. The P300 wave is recorded from EEG(electroencephalogram) electrodes attached on human brain skull after giving alphabetical stimulation. To detect the potential decrease in P300, firstly we statistically model the P300 wave's negative potential. Then we infer human intention based on maximum likelihood estimation. The proposed method was evaluated on the data recorded from three healthy human subjects. The method achieved an averaging accuracy of 98% from subject k, 90% from subject j and 79.8% from subject h.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.8 no.4
• /
• pp.246-251
• /
• 2003

The blood-brain barrier (BBB) is composed of the brain capillaries, which are lined by endothelial cells displaying extremely tight intercellular junctions. Several attempts at creating an in vitro model of the BBB have been met with moderate success as brain capillary endothelial cells lose their barrier properties when isolated in cell culture. This may be due to a lack of recreation of the in vivo endothelial cellular environment in these models, including nearly constant contact with astrocyte foot processes. This work is motivated by the hypothesis that growing endothelial cells on one side of an ultra-thin, highly porous membrane and differentiating astrocyte or astrogliomal cells on the opposite side will lead to a higher degree of interaction between the two cell types and therefore to an improved model. Here we describe our initial efforts towards testing this hypothesis including a procedure for membrane fabrication and methods for culturing endothelial cells on these membranes. We have fabricated a 1 $\mu\textrm{m}$ thick, 2.0 $\mu\textrm{m}$ pore size, and 55% porous membrane with a very narrow pore size distribution from low-stress silicon nitride (SiN) utilizing techniques from the microelectronics industry. We have developed a base, acid, autoclave routine that prepares the membranes for cell culture both by cleaning residual fabrication chemicals from the surface and by increasing the hydrophilicity of the membranes (confirmed by contact angle measurements). Gelatin, fibronectin, and a 50/50 mixture of the two proteins were evaluated as potential basement membrane protein treatments prior to membrane cell seeding. All three treatments support adequate attachment and growth on the membranes compared to the control.

• Journal of Korea Multimedia Society
• /
• v.17 no.12
• /
• pp.1412-1417
• /
• 2014

The corpus callosum is the largest part of the brain, which is related to many neurological diseases. Snake model or active contour model is widely used in medical image processing field, especially image segmentation they look into the nearby edge, localizing them accurately. In this paper, corpus callosum segmentation using the snake model, is proposed. We tested a snake model on brain MRI. Then we compared the result with an active shape approach and found that snake model had better segmentation accuracy also faster than active shape approach.

• The Journal of Internal Korean Medicine
• /
• v.33 no.4
• /
• pp.572-586
• /
• 2012

Objectives : This study was designed to investigate the effects of Sasim-tang (SST) on proinflammatory cytokine production in a photothrombotic ischemia mouse model. Methods : Photothrombotic ischemia was induced in stereotactically held male Balb/c mice using rose bengal (10 mg/kg) and cold light. The target of photothrombotic ischemic lesion was 1 mm anterior to bregma and 3 mm lateral to midline with 2 mm in diameter, which are decreased by oral administration of SST. Results : SST protected ischemic death of brain cells through inhibition of pro-inflammatory cytokines production and catalytic activation of caspase-3 protease in photothrombotic ischemia mouse model. Conclusions : The results of this study suggest that SST can have protective effects on brain cell damage in a photothrombotic ischemia mouse model.