• 한국자원식물학회지
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• 제31권5호
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• pp.522-530
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• 2018

Brachypodium distachyon has been developed as a monocot model plant for temperate grasses and bioenergy crops. Although B. distachyon research is moving forward rapidly, the study of photoresponses has not been explored. To extend our knowledge of responses to light in monocots, we performed photoresponse analysis of B. distachyon using two inbred lines, Bd21 and Bd21-3. In this study, we first compared growing phenotypes between the two lines and investigated coleoptile and primary leaf growths under dark, far-red, red, and white light conditions. The results showed that the growth of the two lines were similar until tillering stage, but other developmental stages from heading to senescence were much delayed in Bd21-3, which resulted in increased height and tiller numbers. Under different light conditions, primary leaf lengths were kept increasing during the growth period, whereas the coleoptile extension was inhibited 4 to 7 days after growth depending on the light conditions applied. These results suggest that the responses to light in B. distachyon can be examined by measuring coleoptile lengths approximately 7 days after seedling growth. Moreover, we selected light-responsive genes known in Arabidopsis thaliana, such as chlorophyll A/B binding protein (CAB), light-harvesting chlorophyll binding protein (Lhcb) and chalcone synthase (CHS), and confirmed their light-induced gene expression in B. distachyon. Therefore, the present study suggests that the inhibition of coleoptile growth can be used as the parameter to analyze photoresponses in the monocot model plant, and also provide the reference genes whose expression is induced by far-red and red light treatment.

• 한국육종학회지
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• 제42권4호
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• pp.351-356
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• 2010

Brachypodium distachyon is rapidly emerged in biological study and has been currently used as a model system for genetics and functional studies for crop improvement and biofuel production. Phosphinothricin (PPT) has been widely used as a selectable agent, which raises ammonium content and induces toxicity in non-transformed plant cells. However PPT selection is not much effective on Brachypodium callus consequently reducing transformation efficiency. In order to identify the efficient conditions of PPT selection, calli obtained from mature seeds of Brachypodium (PI 254867) were cultured on the callus inducing medium (CIM) or regeneration medium (ReM) containing serial dilutions of the PPT (0, 2, 5, 10, and 15 mg/l) in dark or light condition. Callus growth and ammonium content of each treatment were measured 2 weeks after the treatment. Although callus growth and ammonium content did not show much difference in CIM, slow callus growth and increased ammonium accumulation were found in ReM. No significant difference of ammonium accumulation in response to PPT was found between dark and light conditions. In order to identify major factors affecting increased ammonium accumulation, callus was cultured on the media in combined with phytohormones (2,4-D or kinetin) and carbon sources (sucrose or maltose) containing with PPT (5 mg/l). The highest ammonium content in callus was found in the kinetin and maltose media.

• 농업과학연구
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• 제47권2호
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• pp.305-313
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• 2020

Root hair development has the potential to increase crop yields and at the same time to decrease fertilizer use, which will be required in the next 30 years to meet the demand for crop-derived commodities in a world with decreasing available natural resources. Root hair defective six (RHD6) encoding a basic helix-loop-helix transcription factor, is associated with root hair differentiation, and its roles are root hair initiation and elongation. Grass plants, rice and Brachypodium have been used as model plants to study the gene function of the root hair defective six like (RSL) subfamily which is orthologous to AtRHD6. The RSL subfamily has an identical gene function with AtRHD6 which is involved with root hair differentiation as well. Plants with longer root hairs within a species should have an improved Pi uptake efficiency; therefore, we would expect that a plant with a high Pi uptake could contribute to increasing the plant yield. We achieved increased root hair length by manipulating the RSL subfamily genes. It is expected that in these transgenic plants, the long root hairs would be sufficient to improve the Pi uptake and hence improve biomass and yield component (tiller, spikelet number, and spikelet weight) of the plant. Here, we demonstrate that Brachypodium transgenic plants overexpressing the BdRSL subfamily genes have an improved biomass and grain yield. The result of this study could be applied to important crop plants like rice.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 춘계학술발표회
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• pp.138-139
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• 2011