• Title/Summary/Keyword: Bottle ground

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Absence of AVP1 transcripts in wild type watermelon scions grafted onto transgenic bottle gourd rootstocks

  • Kim, Byung Oh;Han, Jeung-Sul;Park, Kyung Il;Jeon, Su Min;Kim, Chang Kil
    • Journal of Plant Biotechnology
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    • v.42 no.1
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    • pp.13-18
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    • 2015
  • In this study we confirmed the stable integration of Arabidopsis AVP1 in the genomes of bottle gourd $T_3$ homozygous lines and its transcription, and additionally evaluated possibility of translocation of the AVP1 mRNA from transgenic bottle gourd rootstocks to wild type watermelon scions. Each AVP1 gene in two bottle gourd T3 lines is abundantly expressed under a field condition. Given the grafting between wild type watermelon scions and AVP1-expressing bottle gourd rootstocks, no translocation of the AVP1 mRNA was detected in leaves, both sexual flowers, and fruits of the scions.

A Study on The Pattern shape(Figure) of the korean Trouser, Hanbok-Baji, in the Realation to its Fabric(Ground) - Emphasized on the Topological Aspect - (한복구조에 나타난 ′옷감′(ground)과 ′옷꼴′(figure)에 관한 연구-위상기하학적 측면을 중심으로-)

  • 임영자;문공화
    • Journal of the Korean Society of Costume
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    • v.52 no.3
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    • pp.149-160
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    • 2002
  • Symbolism found in a certain object inherits characteristics from the culture that contains the objects. The purpose of this study was investigate the formative beauty of Korean trousers twisted the pieces together based on the way of Korean thinks and the Topology. The shape is formed by cutting from the fabric. From the perspective of semiology, the fabric and the pattern shape correspond to ground and figure. Ground and figure are identical with the principle of the whole and the part, which is the same in Korea, China, and the West. But In Korea, the 3-dimensional garment is made by adding a twist. This is very important and defines the difference in the way of thinking and topology. Korean trousers consist of three parts : Hury, Marupok, and Sapok. The small Sapok can be made by removing the Marupok and large Sapok (figure) from the fabric (ground) when making Korean trousers. A Mobius strip is made when the large Sapok is adjoined with the small Sapok by reversing the small Sapok, making a 180$^{\circ}$twist and then stitching together. The theory of Mobius strip can be applied in Joining Bajiburi. thus when the trousers are completed the Klein's bottle is seen because of the 2 existing Mobius strop. The theory of Mobius strip can be applied in Joining Bajiburi, thus when the trousers are completed the Klein's bottle is seen because of the 2 existing Mobius strip. Hury is cylinder while the small and large Sapok make up the Mobius strip. As a result, Mobius strip, Kleins'bottle, protective plan can be applied in cutting Hanbok used in the countryside, so I have come to see that the traditional Korean way of thinking is closely related to the theory of topological.

Effect of Sampling and Analytical Methods on the Fibrous Materials from the Ground Water (시료 채취 조건 및 검사방법에 따른 지하수내 섬유상 물질 검출 양상에 관한 연구)

  • Kim, Ji-Yong;Kim, Jung Ran;Cheong, Hae-Kwan;Lim, Hyun-Sul;Paik, Nam-Won
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.7 no.2
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    • pp.209-222
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    • 1997
  • Authors surveyed the ground water near the waste disposed from a fiberglass production factory to confirm the presence of glassfiber in the water and to determine the effect of sampling conditions and storage on the recovery of fibrous materials in the ground water. Sample was collected at every 4 hours for 48 hours consecutively. After finishing the 48 hours sample, water sampling was done from each tap after repeated turning on and off the water for 30 seconds at each time. Sample was collected in the two 1.5 liter polyethylene bottle after vigorously shaking the bottle with the same water several times with the flowing tap water. At each paired sample, one bottle was stored stand still at room temperature, and the other sample was filtered immediately after sampling. Water was filtered on the Mixed Cellulose Ester filter with negative pressure. Each sample was divided into upper and lower layer. The other bottle was stored at room temperature standstill for 7 days and filtered in the same fashion as the other pair of sample did. Each MCE filter was divided into 4 pieces and one piece was treated with acetone to make it transparent. Each prepared sample was observed by two researchers under the light and polarizing microscopy, scanning electron microscopy and energy dispersive X-ra microanalysis. Fibers were classified by the morphology and polarizing pattern under the polarizing microscope, and count was done. 1. There was a significant fluctuation in number of the fibers, but there was no specific demonstrable pattern. 2. Non-polarizing fibers frequently disappeared after 7 days's storage. But cluster of fibers were found at the wall of the same container by scratching technique. 3. Polarizing fibers were usually found in between the filter and the manicure pasted area. Possible explanations for this phenomenon will be that either these fibers are very light or have electronic polarity. Hence, these fibers are not able to be attached on the surface of slide glass. 4. Under the scanning electron microscopic examination, the fibers which are not refractive under the light microscopy were identified as glassfiber. Other fibers which is refractive under the polarizing microscopy were identified as magnesium silicate fibers. It is strongly suggested that development of standardized method of sample collection and measurement of fibrous material in the water is needed.

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Detection of Cucumber green mottle mosaic virus in Bottle Gourd Seeds by RT-PCR (RT-PCR에 의한 박 종자의 오이녹반모자이크바이러스 검정)

  • Lee, Sook-Kyung;Song, Wan-Yeob;Kim, Hyung-Moo
    • Research in Plant Disease
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    • v.10 no.1
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    • pp.53-57
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    • 2004
  • Cucumber green mottle mosaic virus (CGMMV) was a major pathogen of watermelon and had affected seriously to watermelon production in Korea. Rapid and sensitive detection method of CGMMV associated with bottle gourd (Lagenafia siceraria) seeds was developed by using RT-PCR in this study. A pair of primeri Wmfl and Wmrl, specific for CGMMV was designed from coat protein gene sequences of CGMMV-W and used for amplifying 420 bp product in RT-PCR. To simplify the virus extraction procedure and reduce an inhibitor from the extract for the RT-PCR, some methods using ethanol precipitation, double filtration, polyethylene glycol precipitation and phenol/chloroform/isoamyl alcohol extraction procedure were compared and the phenol/chloroform/isoamyl alcohol extraction procedure was selected by its enhanced sensitivity. This detection method using the selected extraction step and the primers for RT-PCR could reliably detect an infected level of one CGMMV-infested seed in 1,000 seeds. This rapid and sensitive RT-PCR assay provides auseful tool for the specific detection of CGMMV in bottle gourd seed samples containing high levels of back-ground inhibitors.

Estimation of the Ground Surface Roughness Applied by Acoustic Emission Signal (AE 신호를 이용한 연삭 가공물의 표면 거칠기 예측)

  • 곽재섭;송지복
    • Journal of the Korean Society for Precision Engineering
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    • v.17 no.4
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    • pp.240-246
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    • 2000
  • An in-process estimation of the ground surface roughness is a bottle-neck and an essential field in conventional grinding operation. We defined the dimensionless average roughness factor (D.A.R.F) that exhibits a roughness characteristics of ground surface. The D.A.R.F was composed easily of the absolute average and the standard deviation values which were the analytic parameters of the acoustic emission (AE) signal generated during the machining process. The theoretical equation between the surface roughness and the D.A.R.F has been derived from the linear regressive analysis and verified its availability through the experimentation on the surface grinding machine.

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Extraction of the Surface Roughness in Grinding Operation by Acoustic Emission Signal (AE 신호에 의한 연삭가공 표면거칠기 검출)

  • Chung, Sung-Won
    • Journal of the Korean Society of Industry Convergence
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    • v.2 no.2
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    • pp.147-153
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    • 1999
  • An in-process extraction method of the ground surface roughness is a bottle-neck and essential field in conventional machining process. We define the D.A.R.F(Dimensionless Average Roughness Factor) that has a roughness characteristic of ground surface. D.A.R.F include the absolute average and the standard deviation values which are the analytic parameters of the AE(Acoustic Emission) signal generated during the grinding operation. The theoretical equation between the surface roughness and the D.A.R.F has been derived from the linear regressive analysis and verified its availability through the experimentation on the surface grinding machine.

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A STUDY ON BONE STRENGTH AND THE DEGREE OF CONVERSION OF DENTIN BONDING AGENTS (수종 상아질 접착제의 결합강도와 중합률에 관한 연구)

  • Kim, Byung-Hyun;Kwon, Hyuk-Choon
    • Restorative Dentistry and Endodontics
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    • v.23 no.1
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    • pp.68-93
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    • 1998
  • The physical properties of polymer are greatly influenced by the extent to which a resin cures. The presence of un reacted monomer can, have a plasticizing effect on the polymer, thereby altering the physical and mechanical properties of dentin bonding agent (DBA). If the DBA does not polymerize sufficiently, it will leave a weak bonding layer and lead to lower bond strength. The purpose of this study was to evaluate the shear bond strengths(SBS) and the degree of conversion (DC) of 4 commercialy avilable dentin bonding systems which are composed of 2 multi-bottle systems [Scotchbond Multi-Purpose (SMP), AeliteBond(AB)] and 2 onebottle systems [SingleBond(SB), One-Step(OS)]. For shear bond strength measurement, labial surfaces of freshly extracted bovine incisors were ground with # 600 grit SiC paper to expose dentin. Four different groups of samples were formed, with 10 samples. being made for each of the 4 commercial DBA in each group according to the curing sequences of DBA and overlayer thickness of composites: Group I (standard cure and 1mm thick composites) : The DBA was light cured and the composites of 1mm thickness was applied ; Group II (standard cure and 2mm thick composites) : The DBA was light cured and the composites of 2mm thickness was applied; Group III (simultaneous cure and 1mm thick composites) : The DBA was not light-cured and simultaneously cured with composites of 1mm thickness; Group N (simultaneous cure and 2mm thick composites) : The DBA was. not light-cured and simultaneously cured with composites of 2mm thickness. The SBS was measured immediately after the composites was bonded to the bovine dentin using an Instron machine. The DC of the DBA was examined in a thin film under simulated conditions of the experimental groups according to the curing sequences and overlayer thickness of composites in the SBS test. using a Fourier transform Infrared(FTIR) spectrometer. The following results were obtained from SBS tests and DC measurements 1. In SBS tests, the multi-bottle DBA(SMP, AB) had a generally higher bond strength values than the one bottle DBA(SB, OS). In DC measurements, the one bottle DBA(SB, OS) had a significantly higher DC than the multi-bottle DBA(SMP, AB). 2. In all DBAs except OS, there was no significant difference between the bond strength of group I (standard cure and 1mm thick composites) and that of group III (simultaneous cure and 1mm thick composites). SMP, SB in Group I had a significantly higher DC than those in group III, but AB, OS in group I had a significantly lower DC than those in group III 3. All DBAs in Goup II (standard cure and 2mm thick composites) had significantly higher bond strength and DC than those in Group N (simultaneous cure and 2mm thick composites). 4. In all DBAs, there was no significantly different SBS and DC between Group I and Group II, but all DBAs in Group III had significantly higher SBS and DC than those in Group IV.

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Effects of Low Dose Gamma Radiation and Seed Moisture Content on Germination and Early Growth of Vegetable Crops (저선량 방사선 조사 시 종자수분함량이 채소류의 발아와 초기생장에 미치는 영향)

  • Baek, Myung-Hwa;Lee, Young-Keun;Lee, Young-Bok;Yang, Seung-Gyun;Kim, Jae-Sung
    • Korean Journal of Environmental Agriculture
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    • v.22 no.3
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    • pp.215-219
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    • 2003
  • To investigate the effects of low dose gamma radiation and seed moisture content (SMC) on germination and early growth of vegetable crops, seeds of chinese cabbage (Brassica campestris L.), radish (Raphanus sativus L.), red pepper (Capcicum annuum L.), figleaf gourd (Cucurbita ficifolia Bouche) and bottle gourd (Lagenaria siceraria Standl), with different SMC were irradiated with different doses ($0{\sim}20\;Gy$) of gamma-ray by irradiator ($^{60}Co$, ca.150 TBq of capacity, AECL). Vegetable crops in which low dose gamma radiation was irradiated in seeds with different moisture content showed different response in seed germination and early growth to low dose gamma radiation. The germination rate of chinese cabbage, figleaf ground and bottle gourd irradiated with $2{\sim}8\;Gy$ showed interactive responses against relative SMC. Also, significant interactions occurred for the early growth between those factors. The stimulating effects of gamma radiation were more pronounced for hydrated seeds of chinese cabbage, radish, figleaf gourd and bottle gourd showing prominent responses with $2{\sim}10\;Gy$ irradiation, particularly for chinese cabbage and bottle gourd. These results suggest that radiation may promote germination and early growth of vegetable crops through interaction with SMC.

Study on Water Quality of Spring Water in Seoul (서울지역 약수터의 수질특성에 관한 연구)

  • Kim, Kwang-Rae;Gil, Hae-Kyung;Kim, Hyun-Kook;Kim, Eun-Sook;Roh, Bang-Sik;Hong, Ju-Hee;Lee, Jin;Kim, Jeong-Yeon;Lee, Man-Ho;Eom, Seok-Won;Lee, Jai-Young
    • Journal of Soil and Groundwater Environment
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    • v.15 no.6
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    • pp.99-106
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    • 2010
  • We investigated springs in Seoul in 2009 to know the change of water quality according to storage method of spring water, the concentration of chemical compounds and their correlation. Even spring water that originally satisfied national standard for drinking water could be exceeded national standard for drinking water by storage method such as storage bottle, temperature and period; especially used PET bottles could affect the increase of total colony counts. Therefore, spring water is desirable to be consumed on the spot, or to be stored in sterilized bottles in refrigerator rather than room temperature at home, and also to be consumed shortly not exceeding 24 hours. Total colony counts, coliform, yersinia, $F^-$, $Cl^-$, $NO_3^--N$, hardness, total Solids, pH, color and Al were exceeded national standard for drinking water at some springs. The result of correlation analysis shows that hardness and total solids, which are caused by several ionic compounds, had relatively high correlations with other chemical compounds.

Yield characteristics and medium composition of winter mushroom in cultivation farms in Korea (국내 팽이버섯 재배농가의 배지조성 및 수량 특성)

  • Lee, Chan-jung;Lee, Eun-ji;Park, Hye-sung;Lim, Ji-hoon;Min, Gyeong-Jin;Kong, Won-Sik
    • Journal of Mushroom
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    • v.18 no.1
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    • pp.120-124
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    • 2020
  • This study was conducted to provide basic data for setting a standard medium for winter mushroom cultivation. Investigation of medium composition in winter mushroom farms in Korea revealed that the types of medium used for each farm were slightly different and that the mixing ratio of the medium also varied. All farmers used corncob, rice bran, and beet pulp as the main media, and calcium carbonate and ground oyster shells were used at 1.0~3.8% to adjust the pH of the medium. Analysis of the physicochemical properties of the mixed media showed nitrogen content of 1.28~1.52%, carbon content of 45.7~48.5%, and C/N ratio of 30.7~37.9. The content of inorganic components was significantly different in each farm, depending on the type and amount of minerals in the mixed medium. Mycelial growth was the fastest at Farm 2, reaching 6.5 cm within 34 days of culture, followed by Farm 4 at 5.7 cm, whereas Farm 1, 3, and 5 showed similar growth. Mycelial density was high without any significant difference between farms. Survey of yield according to medium composition in each farm showed the highest yield in Farm 2 at 173.4 g/bottle, followed by Farm 1 at 168.4 g/bottle, whereas Farm 3 and 4 showed similar yield. However, Farm 4 had the lowest yield at 145.4 g/bottle. Therefore, mushroom yield was different depending on the medium composition in the farm, and thus selection of a medium that can be used universally in multiple mushroom farms was considered possible.