• The Korean Journal of Mycology
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• v.34 no.1
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• pp.59-61
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• 2006

Gray mold caused by Botrytis cinerea on Agastache rugosa was occurred at a field of Jinju from 2003 to 2005. The disease symptoms started with water-soaked lesions in the leaves and stem, then these infected lesions became withered, discolored, rotten and died eventually. The fungal pathogen was isolated from the lesions. The fungal conidia were one-celled and mostly ellipsoid or ovoid in shape and light gray in color and $4{\sim}20{\times}4{\sim}13\;{\mu}m$ in size. The fungal conidiophores were $14{\sim}28\;{\mu}m$ in width. The Pathogen formed sclerotia abundantly on PDA. The optimum temperature for mycelial growth and sclerotia formation was $20^{\circ}C$. Pathogenicity of the causal organism was proved according to Koch's postulates. On the basis of symptom, mycological characteristics and pathogenicity test, the fungus was identified as Botrytis cinerea. This is the first report on gray mold of Agastache rugosa caused by B. cinerea in Korea.

• The Korean Journal of Mycology
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• v.34 no.2
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• pp.122-124
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• 2006

In March of 2005, gray mold disease caused by Botrytis cinereu on Stachys sieboldii Miq. was occurred in the mud cellar storage of Gyeongsangnam-do Agricultural Research and Extension Services, Korea. The symptoms started with water-soaked and rotten in the tubers. The conidia were one celled and mostly ellipsoid or ovoid in shape and light gray in color. The conidia were $5{\sim}16{\times}4{\sim}12{\mu}m$ in size and the conidiophores were $14{\sim}30{\mu}m$ in length. The pathogen formed conidia and sclerotia abundantly on PDA. The optimal temperature for mycelial growth and sclelotia formation was $20^{\circ}C$. On the basis of symptom, mycological characteristics and pathogenicity test on host plants, the fungus was identified as Botrytis cinerea Persoon: Fries. This is the first report on gray mold of S. sieboldii caused by B. cinerea in Korea.

• Research in Plant Disease
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• v.9 no.1
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• pp.21-24
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• 2003

In May of 2002, the gray mold disease occurred severely on broad bean (Vicia faba) grown in farmer's fields located in Changseon-myon, Namhae-gun, Gyeongnam provice, Korea. The infected leaves, at first, discolored to gray or dark from the tip, and eventually died. Conidia of the fungus in mass were hyaline or gray, 1-celled, mostly ellipsoid or ovoid and sized 6~22$\times$4~16 ${\mu}{\textrm}{m}$. Thick conidiophores were formed on PDA with 15~37 ${\mu}{\textrm}{m}$ in width. The sclerotia were readily formed potato-dextrose agar and optimum temperature for sclerotial formation was 2$0^{\circ}C$. Pathogenicity of the causal organism was proved according to Koch's postulate. The causal organism was identified as Botrytis cinerea based on its mycological characteristics. This is tile first report on gray mold of broad bean (Vicia faba) caused by Botrytis cinerea in Korea.

• The Korean Journal of Mycology
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• v.45 no.3
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• pp.251-257
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• 2017

The occurrence of gray mold on Rubus crataegifolius has recently been reported in Pohang, Gyeongbuk province, Korea. The initial symptom was the appearance of small brown spots on the leaves, and these lesions became later covered with a gray fungus as the disease progressed. A fungus was isolated from symptomatic leaves and incubated. Through morphological and phylogenetic analyses, the causal agent of the disease was identified to be Botrytis cinerea. The fungal isolate was then used to inoculate on the leaves of healthy plants to determine its pathogenicity as the causal agent of gray mold as per Koch's postulates. The inoculated leaves showed the same symptoms as the originally infected plant, and the fungal pathogen re-isolated from the lesions showed the same morphological characteristics as the original pathogen. This is the first report on the occurrence of gray mold on R. crataegifolius caused by B. cinerea in Korea.

• Research in Plant Disease
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• v.23 no.4
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• pp.372-378
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• 2017

In this study, we identified the causative agent of post-harvest gray mold on broccoli that was stored on a farmers' cooperative in Pyeongchang, Gangwon Province, South Korea, in September 2016. The incidence of gray mold on broccoli was 10-30% after 3-5 weeks of storage at $3^{\circ}C$. Symptoms included brownish curd and gray-to-dark mycelia with abundant conidia on the infected broccoli curds. The fungus was isolated from infected fruit and cultured on potato dextrose agar. To identify the fungus, we examined the morphological characteristics and sequenced the rDNA of the fungus and confirmed its pathogenicity according to Koch's postulates. The results of the morphological examination, pathogenicity test, and sequencing of the 5.8S rDNA of the internal transcribed spacer regions (ITS1 and ITS4) and three nuclear protein-coding genes, G3PDH, HSP60, and RPB2, revealed that the causal agent of the post-harvest gray mold on broccoli was Botrytis cinerea. To our knowledge, this is the first report of post-harvest gray mold on broccoli in Korea.

• Research in Plant Disease
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• v.10 no.2
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• pp.117-120
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• 2004

In April of 2003, the gray mold disease occurred severely on statice (Limonium shinuatum Mill. cv. Sophia) grown in the commerical farms in Seosang-myon, Hamyang-gun, Gyeongnam Province, Korea, 2003. The infected plants were started with water-soaking lesions in the stems, the leaves and the flowers became withered and discolored to gray or dark from the tip then gradually expanded to die. The conidia and mycelia of the pathogen appeared on flowers, leaves, calyx and flower stalk. The conidia were gray, I-celled, mostly ellipsoid or ovoid in shape and were 8∼20 x 6∼14 ${\mu}{\textrm}{m}$ in size. Conidiophores were 14∼34 ${\mu}{\textrm}{m}$ in size. The sclerotia were formed abundantly on potato-dextrose agar. The optimum temperature for sclerotial formation was 2$0^{\circ}C$. Pathogenicity of the causal organism was proved according to Koch's postulate. The causal organism was identified as Botrytis cinerea Persoon: Fries based on mycological characteristics. This is the first report on gray mold of statice (Limonium shinuatum) caused by Botrytis cinerea in Korea.

• Research in Plant Disease
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• v.15 no.2
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• pp.127-129
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• 2009

Gray mold caused by Botrytis cinerea occurred on Astragalus sinicus in an experimetal rice field of Gyeongsangnam-do Agricultural Research and Extension Services in Korea from 2008 to 2009. The symptoms appeared on the leaves, petiole and stems of infected plants. and then started with water-soaked, rotted and eventually died blight on plants. The colony were grayish brown and the optimum temperature for sclerotial formation was $20^{\circ}C$ on potato dextrose agar. The conidia were one celled, mostly ellipsoid or ovoid in shape, and were colorless or pale brown in color. The conidia were $7{\sim}16{\times}5{\sim}10{\mu}m$ in size and conidiophores were $14{\sim}28{\mu}m$ in length. On the basis of mycological characteristics and pathogenicity test on host plants, the fungus was identified as Botrytis cinerea Persoon: Fries.

• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.167-170
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• 2008

A fungal strain BCP, which parasitizes Botrytis cinerea gray mold pathogen, was isolated and identified as Acremonium strictum. BCP strain overgrew the colonies of B. cinerea and caused severe lysis of the host hyphae. Frequent penetration and hyphal growth of A. strictum BCP inside the mycelia of B. cinerea were observed under light microscopy. In addition, some morphological abnormalities such as granulation and vacuolation of the cytoplasm were observed in mycelia and spores of B. cinerea. In dual culture test, A. strictum BCP strongly inhibited the mycelial growth of several plant pathogenic fungi as well as B. cinerea. To our knowledge, this is the first report on mycoparasitism of Acremonium species on B. cinerea.

• The Plant Pathology Journal
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• v.17 no.6
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• pp.357-360
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• 2001

Gray mold was observed on leaves of castor bean grown in Woniu and Okcheon in Korea in October 2000. Symptoms developed in the form of spot and blight with sporulation of the causal fungi at the marginal or central parts of the leaves. A total of 25 isolates were obtained from the infected leaves of castor bean. Out of the 25 isolates, 5 isolates which originated from Woniu were identified as Botrytis cinerea, while 20 isolates from Okcheon were identified as Amphobotrys ricini based on morphological and cultural characteristics. Two isolates each of B. cinerea and A. ricini were tested for their pathogenicity to castor bean plants. Gray mold symptoms similar to those observed in the fields were induced on leaves of castor bean by artificial inoculation. This is the first report of gray mold in castor bean caused by B. cinerea and A. ricini in Korea.

• The Plant Pathology Journal
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• v.21 no.1
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• pp.59-63
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• 2005

Bacillus subtilis S1-0210 was selected as a biological agent against Botrytis cinerea in strawberry. The isolate inhibited mycelial growth of B. cinerea in vitro tests. A wettable powder formulation of B. subtilis S1-0210 significantly reduced infection rates with lower than 5%, compared with higher than 70% of infection rates in untreated control. The formulation showed 85 to 89% control efficacies of gray mold incidences on fruits of strawberry in pots. Pre-treatment of the agent was more effective in controlling gray mold on fruits and leaves than post-treatment at the early stage of disease development. The formulation also showed 70% control efficacy of gray mold incidence on fruits of strawberry in a field trial. The results indicate that B. subtilis S1-0210 in the wettable powder formulation may be a potential biocontrol agent to control gray mold on strawberry.