• 식물병연구
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• 제8권1호
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• pp.63-65
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• 2002

2001년 경상남도 남해군 창선면 잠두 재배포장에서 잎과 줄기에 붉은점무의 병이 심하게 발생하였다. 이 병의 병징은 잎에 원형의 붉은점무늬가 많이 생기고 병반의 크기는 1~4.2mm이며, 줄기에는 가늘고 긴 방추형 모양의 병반이 생기고 크기는 1~l5mm이었다. 포장에서 발생율은 16.4~84.6%이 었다. 분생포자와 분생포자병은 PDA배지 상에서 잘 형성되지 않았으나, PDA배지에 잠두 잎 추출액을 첨가하였을 때는 보다 양호하게 형성되었다. 분생포자는 원형 또는 난형이며 담황록색고 크기는 10~24$\times$8~22 $\mu$m 이었다. 분생포자병은 원통형, 담갈색이고 크기는 3.6~12.8 $\mu$m이었다. 균사생육은 적온은 2$0^{\circ}C$에서 균핵 형성은 15$^{\circ}C$-2$0^{\circ}C$에서 가장 잘 형성하였고, 검은색이고 구형 또는 부정형이며, 균핵의 크기는 1.2-11.8mm였다. 병원균의 균학적 특징과 병원성을 검정한 결과 이 병을 Botrytis fabae에 의한 잠두 붉은점무늬병으로 명명하고자 제안한다.

• Mycobiology
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• 제29권4호
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• pp.198-204
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• 2001

Analysis of leaf exudates of Vicia faba using paper chromatography to identify individual amino acids and sugars qualitatively was investigated. The results revealed that the number of identified amino acids detected in the leaf exudates of the susceptible plants was more than those of resistant plants. The results also showed an increase in the number of amino acids exuded by infected leaves, but no marked difference in sugars of infected and non infected plants. Lithium chloride application led to decrease in amino acid and sugar contents. The number of amino acids and sugars was also decreased with leaf age. Botrytis fabae and the selected fungal species(Alternaria alternata, Fusarium oxysporum and Aspergillus niger) were used to show the effect of individual amino acid and sugar on their spore germination. It was observed that all amino acids stimulated the fungal spore germination except serine which inhibited its spore germination. In case of A. alternata, spore germination was stimulated by all amino acids except serine, alanine, glutamic acid, arginine and methionine which caused the inhibition. In case of F. oxysporum, aspartic and glutamic acids inhibited spore germination but the other amino acids stimulated its spore germination. Aspartic acid and phenyl alanine inhibited the spore germination of A. niger. All the identified sugars(galactose, glucose, fructose and rhamnose) stimulated spore germination of all tested fungi.

• The Plant Pathology Journal
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• 제30권4호
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• pp.355-366
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• 2014

In this study, resistance responses were investigated during the interaction of Botrytis fabae with two faba bean cultivars expressing different levels of resistance against this pathogen, Nubaria (resistant) and Giza 40 (susceptible). Disease severity was assessed on leaves using a rating scale from 1 to 9. Accumulation levels of reactive oxygen species (ROS), lipid peroxidation and antioxidant enzymes (superoxide dismutase, catalase and ascorbate peroxidase) were measured in leaf tissues at different times of infection. The expression profiles of two pathogenesis-related proteins (PRPs) encoded by the genes PR-1 and ${\beta}$-1,3-glucanase were also investigated using reverse transcription RT-PCR analysis. The accumulation of these defense responses was induced significantly in both cultivars upon infection with B. fabae compared with un-inoculated controls. The resistant cultivar showed weaker necrotic symptom expression, less ROS accumulation, a lower rate of lipid peroxidation and higher activity of the enzymatic ROS scavenging system compared with susceptible cultivar. Interestingly, ROS accumulated rapidly in the resistant leaf tissues and peaked during the early stages of infection, whereas accumulation was stronger and more intense in the susceptible tissues in later stages. Moreover, the response of the resistant cultivar to infection was earlier and stronger, exhibiting high transcript accumulation of the PR genes. These results indicated that the induction of oxidant/antioxidant responses and the accumulation of PRPs are part of the faba bean defense mechanism against the necrotrophic fungus B. fabae with a different intensity and timing of induction, depending on the resistance levels.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.25-27
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• 2003

Since 1993, a total of 50 problematic plant diseases unrecorded in Korea were surveyed in Gyeongnam province. Totally 34 new host plants to corresponding pathogens investigated in this study were 5 fruit trees, 9 vegetables, 12 ornamental plants, 3 industrial crops, and 5 medicinal plants. Among the newly recorded fruit tree diseases, fruit rot of pomegranate caused by Coniella granati and Rhizopus soft rot of peach caused by Rhizopus nigricans damaged severely showing 65.5% and 82.4% infection rate. Among the vegetable diseases, corynespora leaf spot of pepper caused by Corynespora cassiicola and the crown gall of pepper caused by Agrobacterium tumefaciens, powdery mildew of tomato caused by Oidiopsis taurica were the most severe revealing 47.6%, 84.7%, and 54.5% infection rate in heavily infected fields, respectively. In ornamental plants, collar rot of lily caused by Sclerotium rolfsii, gray mold of primula caused by Botrytis cinerea, soot leaf blight of dendrobium caused by Pseudocercospora dendrobium, sclerotinia rot of obedient plant caused by Sclerotinia sclerotiorum showed 32.7 to 64.8% disease incidence. On three industrial plants such as sword bean, broad bean, and cowpea, eight diseases were firstly found in this study. Among the diseases occurring on broad bean, rust caused by Uromyces viciae-fabae and red spot caused by Botrytis fabae were the major limiting factor for the cultivation of the plant showing over 64% infection rate in fields. In medicinal plants, anthracnose of safflower caused by Collectotrichum acutatum was considered the most severe disease on the plant and followed by collar rot caused by Sclerotium rolfsii.(중략)

• Bulletin of the Korean Chemical Society
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• 제32권11호
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• pp.3996-4000
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• 2011

In an attempt to find a new class of anti microbial agents, a series of thiazolidinone and their 5-arylidene derivatives containing 4-(4-methyl benzamido)-benzoyl moiety were synthesized via the reaction of benzocaine with appropriate chemical reagents. These compounds were screened for their antibacterial activity against Gram-positive bacteria (Bacillus subtilis and Bacillus thuringiensis), Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa) and antifungal activity against Botrytis fabae, Fusarium oxysporan and Candida albicans. On the other hand the synthesized compounds were also screened for their anti tubercular activity. IR, $^1H$ NMR, $^{13}C$ NMR and MS spectral analyses established the structures of the newly synthesized compounds. The results revealed that some of these compounds have shown promising antimicrobial and anti tubercular activity in comparison with standard drugs.

• 미생물학회지
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• 제42권2호
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• pp.135-141
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• 2006

덩굴마름병균인 Didymella bryoniae KACC 40669에 대하여 높은 항진균 활성을 나타내는 Brevibacillus sp. KMU-391은 Russia의 Sanktpeterburg 지역의 토양 시료로부터 분리하였다. Brevibacillus sp. KMU-391이 생산하는 항진균 물질은 D. bryoniae의 생육을 강하게 억제할 수 있었으며 TSB를 기본배지로 하여 탄소원으로 1.0% sucrose와 1.0%의 polypeptone을 첨가한 후 pH를 7.0으로 조정하여 $30^{\circ}C$에서 180 rpm으로 2일간 배양하였을 때 가장 높은 항진균 활성을 나타내었다. 배양액에 $30{\sim}60%$의 ammonium sulfate를 처리하여 Brevibacillus sp. KMU-391이 생산하는 항진균 물질을 회수한 후 D. bryoniae를 액체배양을 통한 항진균 활성을 조사한 결과 대조구와 비교하여 41%의 생육을 저해하는 것으로 확인되었다. 또한 butanol을 이용하여 항잔균 물질을 회수한 후, 다양한 작물병원성 곰팡이를 대상으로 항진균 활성 spectrum을 조사한 결과 조사, 장미 잿빛곰팡이병, 잠두 붉은점무늬병, 고추 탄저병, 참외 탄저병, 수박 등의 박과작물의 덩굴마름병, 보리 질병, 토마토 시들음병, 글라디올러스 마른썩음병, 토마토 질병, 수박 덩굴쪼김병, 수박 질병, 사과나무 역병, 벼 잎집무늬마름병, 참외 줄기썩음병, 그리고 고추균핵병에 대하여 높은 항진균 활성을 나타내었다. 이러한 결과를 바탕으로 Brevibacillus sp. KMU-391을 이용하여 작물병원성 곰팡이에 대한 생물농약으로서의 응용 가능성을 확인 할 수 있었다.