• 제목/요약/키워드: Botany

검색결과 808건 처리시간 0.023초

Comparative Proteome Analysis of Two Antagonist Bacillus subtilis Strains

  • Zhang, C.X.;Zhao, X.;Han, F.;Yang, M.F.;Chen, H.;Chida, T.;Shen, S.H.
    • Journal of Microbiology and Biotechnology
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    • 제19권4호
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    • pp.351-357
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    • 2009
  • Natural wild-type strains of Bacillus subtilis are extensively used in agriculture as biocontrol agents for plants. This study examined two antagonist B. subtilis strains, KB-1111 and KB-1122, and the results illustrated that KB-1122 was a more potent inhibitor of the indicator pathogen than KB-1111. Thus, to investigate the intrinsic differences between the two antagonist strains under normal culture conditions, samples of KB-1111 and KB-1122 were analyzed using MALDI-TOF-MS. The main differences were related to 20 abundant intracellular and 17 extracellular proteins. When searching the NCBI database, a number of the differentially expressed proteins were identified, including 11 cellular proteins and 10 secretory proteins. Among these proteins, class III stress-response-related ATPase, aconitate hydratase, alpha-amylase precursor, and a secretory protein, endo-l, 4-beta-glucanase, were differentially expressed by the two strains. These results are useful to comprehend the intrinsic differences between the antagonism of KB-1111 and KB-1122.

Differential responses of two rice varieties to salt stress

  • Ghosh, N.;Adak, M.K.;Ghosh, P.D.;Gupta, S.;Sen Gupta, D.N.;Mandal, C.
    • Plant Biotechnology Reports
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    • 제5권1호
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    • pp.89-103
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    • 2011
  • Two rice varieties, viz. Nonabokra and Pokkali, have been evaluated for their responses to salinity in terms of some physiological and biochemical attributes. During the exposure to salinity (200 mM concentration of sodium chloride for 24, 48, and 72 h), a significant increase in sodium was recorded which was also concomitant with the changes of other metabolic profiles like proline, phenol, polyamine, etc. The protein oxidation was significantly increased and also varied between the two cultivars. The changes in activities of anti-oxidative enzymes under stress were significantly different to the control. The detrimental effects of salinity were also evident in terms of lipid peroxidation, chlorophyll content, protein profiles, and generation of free radicals; and these were more pronounced in Pokkali than in Nonabokra. The assessment and analysis of these physiological characters under salinity could unravel the mechanism of salt responses revealed in this present study and thus might be useful for selection of tolerant plant types under the above conditions of salinity.

A Survey of Termitomyces (Lyophyllaceae, Agaricales), Including a New Species, from a Subtropical Forest in Xishuangbanna, China

  • Ye, Lei;Karunarathna, Samantha C.;Li, Huli;Xu, Jianchu;Hyde, Kevin D.;Mortimer, Peter E.
    • Mycobiology
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    • 제47권4호
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    • pp.391-400
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    • 2019
  • A survey of mushrooms was conducted in Xishuangbanna, Yunnan Province, China, in the rainy season (May to October) of 2012, 2013, and 2014, during which 16 specimens of Termitomyces were collected. Preliminary macro- and micro-characteristics, together with ITS sequence data, showed that four of the specimens belonged to a new species (Termitomyces fragilis), while the other 12 belonged to T. aurantiacus, T. eurrhizus, T. globules, T. microcarpus, and T. bulborhizus. In this paper, T. fragilis is introduced as a species new to science based on morphological characterization and phylogenetic analyses. Macro- and micro- morphological descriptions, color photographs and line drawings of the new species, and a phylogenetic tree to show the placement of the new species are provided. T. fragilis is then compared with other closely related taxa in the genus Termitomyces.

Effect of nitrogen sources and 2, 4-D treatment on indirect regeneration of ginger (Zingiber officinale Rosc.) using leaf base explants

  • Mehaboob, Valiyaparambath Musfir;Faizal, Kunnampalli;Raja, Palusamy;Thiagu, Ganesan;Aslam, Abubakker;Shajahan, Appakan
    • Journal of Plant Biotechnology
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    • 제46권1호
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    • pp.17-21
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    • 2019
  • Ginger is an important monocotyledonous plant belonging to the family Zingiberaceae. The objective of this study was to investigate the regeneration potential of ginger using leaf base explants. Auxins such as 2, 4-D and NAA in combination with BA were used for initiation of callus. Different combinations of both ammonium ($NH^{4+}$) and nitrate ($NO^{3-}$) were also studied for efficient callus production. High frequency of white friable calli was observed on modified Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 2, 4-D, 0.5 mg/L NAA and 0.5 mg/L BA. The highest shoot induction (92.33%), shootlets number ($7.33{\pm}0.33$) and length ($88.33{\pm}4.40$) mm were achieved on MS media containing 0.5 mg/L BA. Regenerated shoots were transferred to in vitro rooting media containing 1.0 mg/L IBA. Afterwards, plantlets with well-developed root and shoot system were subjected to a twostep hardening process. 71% of plantlets survived after secondary hardening without any abnormal morphology.

Selaginella austrotibetica (Selaginellaceae), a new spikemoss from Xizang, China

  • Meng-Hua Zhang;Jie Yang;Aleksandr Petrovich Shalimov;Jong-Soo Kang;Xian-Chun Zhang
    • Journal of Species Research
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    • 제13권1호
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    • pp.1-9
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    • 2024
  • Selaginella austrotibetica, a new spikemoss species from China, is described. The species is epipetric and was found on a moss-covered rocky cliff by a riverbank. Morphologically, it is unlike any anisosporophyllous species in the genus Selaginella. However, it resembles species of the S. sanguinolenta group, especially similar to S. sajanensis in gross morphology, except for the characters of strobilus. The molecular phylogenetic analysis clearly showed that S. austrotibetica was nested within the anisosporophyllous species clade, forming a sister relationship with the clade of S. vaginata and S. xipholepis, and distinct from the S. sanguinolenta group, including S. sajanensis. Although S. austrotibetica is sister to the clade of S. vaginata and S. xipholepis, the species can be clearly distinguished by its sparse leaves on branches, dorsal leaves that are broad-ovate, base obtuse to rounded, margin denticulate or very shortly sparsely ciliolate, and leaves that are slightly thick and shiny. Therefore, we described Selaginella austrotibetica as a new species and discuss its phylogenetic relationships within the genus Selaginella.

Effects of Plant Extracts on Conidial Germination, Mycelial Growth and Sporulation of Fungi Isolated From Poultry Feed

  • Islam, M. Rafiqul;Alam, Shahidul;Rahman, M. Ziaur;Chowdhury, S.P.;Begum, M.F.;Akhter, Nargis;Alam, M.S.;Han, Kee-Don;Lee, Min-Woong
    • Mycobiology
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    • 제31권4호
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    • pp.221-225
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    • 2003
  • Effect of ethanolic extracts of Lawsonia inermis, Azadirachta indica, Vinca rosea, Tagetes patula, Ocimum sanctum, Colocasia antiquorum, Adhatoda vasica, Moringa oleifera, Datura metel and Curcuma longa leaf on conidial germination, mycelial growth and sporulation of Aspergillus flavus, A. niger and A. fumigatus were examined. The conidial germination of A. flavus and A. fumigatus were most inhibited by the extract of L. inermis, while that of A. niger was inhibited by A. indica. Other tested plant extracts have a good effect on conidial germination on the selected fungi. The highest mycelial growth of A. flavus(37 mm) was found in V. rosea, but in case of A. niger and A. fumigatus it(38 and 39 mm) was found in D. metel. The lowest(4, 9 and 6 mm) respectively mycelial growth of these fungi found in L. inermis. The highest sporulation($75{\times}10^4/ml$) of A. flavus was counted in V. rosea, but in case of A. niger and A. fumigatus those($45{\times}10^4$ and $55{\times}10^4/ml$) were in D. metel and the lowest($5{\times}10^4,\;12{\times}10^4\;and\;9{\times}10^4/ml$) respectively sporulation of these fungi counted in L. inermis plant extract medium.

The Effect of $Luteolin-7-O-{\beta}-D-Glucuronopyranoside$ on Gastritis and Esophagitis in Rats

  • Min, Young-Sil;Bai, Ki-Lyong;Yim, Sung-Hyuk;Lee, Young-Joo;Song, Hyun-Ju;Kim, Jin-Hak;Ham, In-Hye;Whang, Wan-Kyun;Sohn, Uy-Dong
    • Archives of Pharmacal Research
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    • 제29권6호
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    • pp.484-489
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    • 2006
  • This Study evaluated the inhibitory action of $luteolin-7-O-{\beta}-D-glucuronopyranoside$, luteolin which was isolated from Salix gilgiana leaves, and omeprazole on reflux esophagitis and gastritis in rats. Reflux esophagitis and gastritis were induced surgically and by the administration of indomethacin, respectively. The intraduodenal administration of $luteolin-7-O-{\beta}-D-glucuronopyranoside$ decreased the ulcer index, injury area, gastric volume and acid output, and increased the gastric pH compared with luteolin. $Luteolin-7-O-{\beta}-D-glucuronopyranoside$ significantly decreased the size of the gastric lesions that had been induced by exposing the gastric mucosa to indomethacin. The malondialdehyde content, which is the end product of lipid peroxidation, was increased significantly after inducing of reflux esophagitis. The malondialdehyde content was decreased by $Luteolin-7-O-{\beta}-D-glucuronopyranoside$ but not luteolin or omeprazole. $Luteolin-7-O-{\beta}-D-glucuronopyranoside$ has a more potent antioxidative effect than luteolin. $Luteolin-7-O-{\beta}-D-glucuronopyranoside$ is a promising drug for the treatment of reflux esophagitis and gastritis.