Age and growth of the mudskipper, Scartelaos gigas were investigated using the second actinost bone of the pectoral girdle, based on an analysis of 560 individuals collected from the mud flats in south west Korea. Specimens were collected semimonthly from March to September of 2003. Actinost and ring radii of each ring group showed a direct one-to-one relationship, with ring radius increasing in tandem with actinost radius. Monthly change in the marginal indices showed that ring formation occurred between May and July(mainly June), being supported by the monthly change in the gonadosomatic(highest in June) and hepatosomatic(lowest in July) indices. Because the species has not been found since November, when air temperature was less than about
Purpose of study; The purpose of this study is specifically classified as two parts. The one is to attempt the chronological annals of Quaternary topographic surface through the study over the formation process of alluvial surfaces in our country, setting forth the alluvial surfaces lower-parts of Han River area, as the basic deposit, and comparing it to the marginal landform surfaces. The other is to attempt the classification of micro morphology based on the and condition premising the land use as a link for the regional development in the lower-parts of Han river area. Reasons why selected the Lower-parts of Han river area as study objects: 1. The change of river course in this area is very serve both in vertical and horizontal sides. With a situation it is very easy to know about the old geography related to the formation process of topography. 2. The component materials of gravel, sand, silt and clay are deposited in this area. Making it the available data, it is possible to consider about not oかy the formation process of topography but alsoon the development history to some extent. 3. The earthen vessel, a fossil shell fish, bone, cnarcoal and sea-weed are included in the alluvial deposition in this area. These can be also valuable data related to the chronological annals. 4. The bottom set conglometate beds is also included in the alluvial deposits. This can be also valuable data related to the research of geomorphological development. 5. Around of this area the medium landform surface, lower landform surface, pediment and basin, are existed, and these enable the comparison between the erosion surfaces and the alluvial surfaces. Approach : 1. Referring to the change of river beds, I have calculated the vertical and horizontal differences comparing the topographic map published in 1916 with that published in 1966 and through the field work 2. In classifying the landform, I have applied the method of micro morphological classification in accordance with the synthetic index based upon the land conditions, and furthermore used the classification method comparing the topographic map published in 1916 and in that of 1966. 3. I have accorded this classification with the classification by mapping through appliying the method of classification in the development history for the field work making the component materials as the available data. 4. I have used the component materials, which were picked up form the outcrop of 10 places and bored at 5 places, as the available data. 5. I have referred to Hydrological survey data of the ministry of Construction (since 1916) on the overflow of Han-river, and used geologic map of Seoul metropolitan area. Survey Data, and general map published in 1916 by the Japanese Army Survbey Dept., and map published in 1966 by the Construction Research Laboratory and ROK Army Survey Dept., respectively. Conclusion: 1. Classification of Morphology: I have added the historical consideration for development, making the component materials and fossil as the data, to the typical consideration in accordance with the map of summit level, reliefe and slope distribution. In connection with the erosion surface, I have divided into three classification such as high, medium and low-,level landform surfaces which were classified as high and low level landform surfaces in past. furthermore I have divided the low level landform surface two parts, namely upper-parts(200-300m) and bellow-parts(
Pluripotency of human embryonic stem cell (hESC) is one of the most valuable ability of hESCs for applying cell therapy field, but also showing side effect, for example teratoma formation. When transplant multipotent stem cell, such as mesnchymal stem cell (MSC) which retains similar differentiation ability, they do not form teratoma in vivo, but there exist limitation of cellular source supply. Accordingly, differentiation of hESC into MSC will be promising cellular source with strong points of both hESC and MSC line. In this study, we described the derivation of MSC like cell population from feeder free cultured hESC (hESC-MSC) using direct differentiation system. Cells population, hESC-MSC and bone marrow derived MSC (BM-MSC) retained similar characteristics in vitro, such as morphology, MSC specific marker expression and differentiation capacity. At the point of differentiation of both cell populations, differentiation rate was slower in hESC-MSC than BM-MSC. As these reason, to verify differentially expressed molecular condition of both cell population which bring out different differentiation rate, we compare the molecular condition of hESC-MSC and BM-MSC using 2-D proteomic analysis tool. In the proteomic analysis, we identified 49 differentially expressed proteins in hESC-MSC and BM-MSC, and they involved in different biological process such as positive regulation of molecular function, biological process, cellular metabolic process, nitrogen compound metabolic process, macromolecule metabolic process, metabolic process, molecular function, and positive regulation of molecular function and regulation of ubiquitin protein ligase activity during mitotic cell cycle, cellular response to stress, and RNA localization. As the related function of differentially expressed proteins, we sought to these proteins were key regulators which contribute to their differentiation rate, developmental process and cell proliferation. Our results suggest that the expressions of these proteins between the hESC-MSC and BM-MSC, could give to us further evidence for hESC differentiation into the mesenchymal stem cell is associated with a differentiation factor. As the initial step to understand fundamental difference of hESC-MSC and BM-MSC, we sought to investigate different protein expression profile. And the grafting of hESC differentiation into MSC and their comparative proteomic analysis will be positively contribute to cell therapy without cellular source limitation, also with exact background of their molecular condition.
자가치아이식이란 자신의 치아를 구강 내의 한 위치에서 다른 발치와나 외과적으로 형성된 치조와로 이동시키는 술식이다. 통상적으로 이 술식은 상실되거나 보존하기 힘든 제1대구치를 다른 치아로 대체하거나, 매복된 견치를 악궁의 정상 위치로 이동시키거나, 전치부와 같이 심미적으로 중요한 부위의 상실된 치아를 소구치로 대체하는 경우에 많이 사용된다. 자가치아이식을 성공적으로 수행하기 위해서는 세심한 수술 기법과 더불어 적절한 증례 선택이 중요한데, 이식할 치아는 치근 발육이 1/2~3/4 정도 이루어진 미성숙 치아가 유리하고 근단공이 넓을수록 치수 재혈관화의 가능성도 높아져 이후의 근관 치료의 필요성도 줄일 수 있다. 본 증례들은 소아에서 치근단 미완성 치아의 자가치아이식을 통해 임상적, 방사선학적으로 양호한 치유 결과를 얻었고, 별도의 근관 치료 없이 양호한 치수 치유 결과를 보였기에 보고하는 바이다.
Purpose: The purpose of this study is to find the effect of rare earth magnet's magnetic field of to the osteoblast around the implant by the means of observation number, and distribution around the implant which is connected to the permanent magnet but not, counted and compared by the number of cells attached to the surface of the implant. Material and method: The permanent magnets, made in the healing cap form, were connected to the implant future, and placed on the culture plate, The osteoblast-like cell: MC3T3-E1 were used for cell culture. As the control group, the implant were connected to normal healing cap, and cultured in the same conditions. 48 hours later, using inverted microscope, the number and distribution of osteoblast around the implant were observed, and 72 hours later, the number of the cells attached to the implant were counted. Results: As a result, the implant connected to the permanent magnet had proved to have a more concentrated cell distribution rate than the control group. The implant connected to the permanent magnet, neck area : which has about 10 gauss magnetic force, had more cells than apex area. The implant connected to the permanent magnet had proven to attach to the osteoblast more productively than control group's implant. Conclusions: This research showed that the magnetic field of the permanent magnet affected the distribution and growth rate of the osteoblast around the implant. In order to support this study, it also had need to monitor the progress of the permanent magnet specifically shown on the neck area, which has10 gauss magnetic force. So after additional research on the distribution and attachment of the cells, and further more, on bone formation, it will be concluded that the clinical applications ,such as immediate loading of implant treatment are possible.
칼슘과 인은 동물의 골격형성 및 반 평형유지에 필수적일 뿐 아니라 란각의 중요간 구성물질이다. 그러나 가금의 주요 원료사료인 식물성사료는 대부분 칼슘과 인의 함량이 낮으며 그 이용성도 낮아 별도로 추가공급이 필요하다. 따라서 칼슘과 인의 기능을 밝히고 요구량을 찾아내며, 그 공급원 및 이용성을 조사하는 것은 매우 중요하다. 이제까지 칼슘과 인의 영향적 특성 및 공급에 관해 고찰하였는 바 결과를 요약하면 다용과 같다. 1. 칼슘과 인은 정상적인 기능을 수행하기 위해 비타민D 및 Na 등과 긴밀한 상호작용을 가지고 있다. 2. 대부분의 식물성 사료내에 들어 있는 인은 피 틴태로서 단위 동물에는 이용성이 낮으며 가축의 년령이 증가하거나 비타민D
치아의 맹출은 일반적으로 치근의 1/2~2/3이 완성(Nolla stage 7)되었을 때 임상적으로 출은(emergence)하여 치근 형성이 완성되기 전에 교합평면에 도달한다. 조기 맹출이란 맹출이 가속화되어 정상 맹출 시기 보다 이른 시기에 맹출하는 것으로, 일반적으로 정상 범주를 벗어나는 영구치의 조기 맹출은 매우 드물며 유치의 만성 치근단 주위염 등의 국소적 원인으로 인한 계승 영구치의 상방 치조골이 광범위하게 파괴되었을 때 치아의 치관이나 치근의 형성이 거의 이루어지지 않은 채 계승 영구치가 조기 맹출하는 것을 관찰할 수 있다. 또한 영구치의 조기 맹출에 영향을 미칠 수 있는 여러 전신 질환이 있는 경우에도 영구치의 조기 맹출이 가능하다. 조기맹출 치아의 임상 소견으로는 심한 동요도와 저작 시 동통, 법랑질의 저석회화, 치아의 편향과 변위, 회전 등이 있으며, 방사선학적 소견으로는 미발육된 치근과 골지지의 부족 등이 있다. 조기 맹출 영구치의 발생 가능한 합병증으로는 만성적인 외상과 동통 및 부종이 있을 수 있고, 맹출 순서의 변경과 인접치의 이동으로 인한 소구치 매복율의 증가, 치아의 변위와 회전이 야기될 수 있으며, 영구치의 탈락도 있을 수 있다. 따라서 증상이 있는 조기 맹출 영구치의 경우 공간 유지 및 영구치 고정과 주위 조직의 회복, 치근 발육의 유도를 위해 적절한 처치가 필요하다. 전신 질환이 없는 만7세 여환이 상악 양쪽 제 1소구치의 심한 동요도와 동통을 주소로 치과 의원에서 의뢰되었다. 임상 및 방사선 검사상 치근 형성이 거의 없이 양측 상악 제 1소구치들이 조기 맹출되어 심한 동요도와 부종 및 저작 시동통을 나타내어 상악 제 2유구치에 교정용 band와 spur를 이용한 구내 고정식 장치를 제작하여 14개월 간 장착하여 조기 맹출한 치아들을 안정화시키고 성공적으로 치근 형성을 도모하였기에 이를 보고하는 바이다.
본 연구팀에서는 방사선에 의한 면역조혈계 및 위장관계 손상에 대한 방호를 위하여 당귀, 천궁, 작약 혼합물의 열수 추출물로부터 그 다당체 함량을 강화한 생약조성물 Hemo HIM을 개발한 바 있다. 본 연구에서는 열수 추출물에 기반을 두어 제조된 HemoHIM에 비하여 지용성 폴리페놀 성분을 강화함으로써 더 뛰어난 생리활성을 갖는 생약조성물을 개발하고자 30% 에탄올 추출과 열수 추출을 함께 시행하여 얻은 추출물을 기반으로 생약조성물 MH-30을 제조하였다. MH-30과 HemoHIM의 에탄올 분획 내의 성분을 HPLC로 비교 분석한 결과 수용성 성분에는 큰 차이가 없었으나 여러가지 지용성 성분이 MH-30에서 크게 증가하는 것을 확인하였으며, 특히 decursin의 함량은 8.7배로 크게 증가하였다. 다음으로 MH-30의 시험관 내 항산화 및 면역세포 활성화 효과와 방사선 조사 마우스에서 면역조혈계 및 재생조직의 방호 효과를 HemoHIM과 비교하여 관찰하였다. 항산화 활성을 비교하기 위한 시험관 내 hydroxyl radical 및 superoxide anion 소거 활성 평가에서 MH-30이 HemoHIM보다 높은 항산화 활성을 보였다. 림프구 증식능을 이용한 시험관 내 면역세포 활성화 시험에서는 MH-30과 Hemo HIM은 거의 비슷한 활성을 나타내었다. 방사선 조사 마우스에서 MH-30은 내재성 비장 조혈세포 집락수를 증가시키고 골수조직 내 세포사멸을 줄였으며, 위장관 재생조직인 소장움의 생존율을 증가시키는 등 방사선에 의한 면역조혈계와 재생조직 손상을 방호하는 효과를 보여주었다. 또한, 종합적인 방사선 방호 효과를 평가하는 지표인 방사선 조사 마우스의 30일 생존율도 MH-30 투여로 유의하게 증가함을 확인하였다. 특히 상기한 모든 마우스 실험에서 MH-30은 Hemo HIM보다 더 뛰어난 방호 효과가 있음을 관찰할 수 있었다. 이상의 결과들은 새롭게 개발한 생약조성물 MH-30이 면역 조혈계와 재생조직의 방사선 손상을 줄여주는 효과가 있으며 기존에 개발된 HemoHIM에 비해 더 뛰어난 활성을 갖고 있음을 보여주었다. 따라서 MH-30은 방사선 사고 또는 암환자의 방사선치료 시 발생할 수 있는 면역조혈계 및 재생조직의 손상을 경감시킬 수 있는 방사선 방호 물질로서 유용하게 활용될 수 있을 것으로 생각한다.
The wall shear stress in the vicinity of end-to end anastomoses under steady flow conditions was measured using a flush-mounted hot-film anemometer(FMHFA) probe. The experimental measurements were in good agreement with numerical results except in flow with low Reynolds numbers. The wall shear stress increased proximal to the anastomosis in flow from the Penrose tubing (simulating an artery) to the PTFE: graft. In flow from the PTFE graft to the Penrose tubing, low wall shear stress was observed distal to the anastomosis. Abnormal distributions of wall shear stress in the vicinity of the anastomosis, resulting from the compliance mismatch between the graft and the host artery, might be an important factor of ANFH formation and the graft failure. The present study suggests a correlation between regions of the low wall shear stress and the development of anastomotic neointimal fibrous hyperplasia(ANPH) in end-to-end anastomoses. 30523 T00401030523 ^x Air pressure decay(APD) rate and ultrafiltration rate(UFR) tests were performed on new and saline rinsed dialyzers as well as those roused in patients several times. C-DAK 4000 (Cordis Dow) and CF IS-11 (Baxter Travenol) reused dialyzers obtained from the dialysis clinic were used in the present study. The new dialyzers exhibited a relatively flat APD, whereas saline rinsed and reused dialyzers showed considerable amount of decay. C-DAH dialyzers had a larger APD(11.70
The wall shear stress in the vicinity of end-to end anastomoses under steady flow conditions was measured using a flush-mounted hot-film anemometer(FMHFA) probe. The experimental measurements were in good agreement with numerical results except in flow with low Reynolds numbers. The wall shear stress increased proximal to the anastomosis in flow from the Penrose tubing (simulating an artery) to the PTFE: graft. In flow from the PTFE graft to the Penrose tubing, low wall shear stress was observed distal to the anastomosis. Abnormal distributions of wall shear stress in the vicinity of the anastomosis, resulting from the compliance mismatch between the graft and the host artery, might be an important factor of ANFH formation and the graft failure. The present study suggests a correlation between regions of the low wall shear stress and the development of anastomotic neointimal fibrous hyperplasia(ANPH) in end-to-end anastomoses. 30523 T00401030523 ^x Air pressure decay(APD) rate and ultrafiltration rate(UFR) tests were performed on new and saline rinsed dialyzers as well as those roused in patients several times. C-DAK 4000 (Cordis Dow) and CF IS-11 (Baxter Travenol) reused dialyzers obtained from the dialysis clinic were used in the present study. The new dialyzers exhibited a relatively flat APD, whereas saline rinsed and reused dialyzers showed considerable amount of decay. C-DAH dialyzers had a larger APD(11.70