• Archives of Pharmacal Research
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• 제25권6호
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• pp.801-806
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• 2002

Cyclicpeptides are important targets in peptide synthesis because of their interesting biological properties. Constraining highly flexible linear peptides by cyclization is one of the mostly widely used approaches to define the bioactive conformation of peptides. Cyclic peptides often have increased receptor affinity and metabolic stability over their linear counterparts. We carried out virtual screening experiment via docking in order to understand the interaction between HLE-Human Leukocyte Elastase and ligand peptide and to identify the sequence that can be a target in various ligand peptides. We made cyclic peptides as a target base on Metlle-Phe sequence having affinity for ligand and receptor active site docking. There are three ways to cyclize certain sequences of amino acids such as Met-lie-Phe-Gly-Ile. First is head-to-tail cyclization method, linking between N-terminal and C-terminal. Second method utilizes amino acid side chain such as thiol functional group in Cys, making a thioether bond. The last one includes an application of resin-substituted amino acids in solid phase reaction. Among the three methods, solid phase reaction showed the greatest yield. Macrocyclization of Fmoc-Met-Ile-Phe-Gly-Ile-OBn after cleavage of Fmoc protection in solution phase was carried out to give macrocyclic compound 5 in about 7% yield. In the contrast with solution phase reaction, solid phase reaction for macrocyclization of Met-Ile-Phe-Gly-Ile-Asp-Tentagel in normal concentrated condition gave macrocyclic compound 7 in more than 35% yield.

• Earthquakes and Structures
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• 제9권4호
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• pp.831-849
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• 2015

Seismic-design procedures for walls require that the confinement in the critical (plastic hinge) regions should extend over a length in the compression zone of the cross section at the wall base where concrete strains in the Ultimate Limit State (ULS) exceed the limit of 0.0035. In a performance-based framework, confinement is linked to required curvature ductility so that the drift demand at the performance point of the structure for the design earthquake may be met. However, performance of flexural walls in the recent earthquakes in Chile (2010) and Christchurch (2011) indicates that the actual compression strains in the critical regions of many structural walls were higher than estimated, being responsible for several of the reported failures by toe crushing. In this study, the method of estimating the confined region and magnitude of compression strain demands in slender walls are revisited. The objective is to account for a newly identified kinematic interaction between the normal strains that arise in the compression zone, and the lumped rotations that occur at the other end of the wall base due to penetration of bar tension yielding into the supporting anchorage. Design charts estimating the amount of yield penetration in terms of the resulting lumped rotation at the wall base are used to quantify the increased demands for compression strain in the critical section. The estimated strain increase may exceed by more than 30% the base value estimated from the existing design expressions, which explains the frequently reported occurrence of toe crushing even in well confined slender walls under high drift demands. Example cases are included in the presentation to illustrate the behavioral parametric trends and implications in seismic design of walls.

• 한국구조물진단유지관리공학회 논문집
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• 제23권4호
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• pp.131-136
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• 2019

굳지 않은 콘크리트의 적층성은 콘크리트 3D 프린팅의 핵심 요소로써 필라멘트의 변형 및 붕괴 없이 일정한 높이로 적층하는 성능이다. 적층성은 항복응력과 밀접한 관련이 있으며, 항복응력이 높을수록 우수하다. 또한, 굳지 않은 콘크리트는 압출된 후 시간경과에 따라 경화되면서 전단응력이 증가하기 때문에 적층성이 높아지게 된다. 따라서, 콘크리트 3D 프린팅 시 굳지 않은 콘크리트의 적층성 확보를 위해서는 출력되는 레이어 사이의 적절한 시간 간격(Printing Time Gap, 이하 PTG)이 필요하다. PTG가 증가함에 따라 적층성은 증가하지만, PTG가 과다하게 커지면 출력된 레이어 간의 부착성능이 감소하며, 출력시간이 길어짐에 따라서 압출성능이 저하될 수 있다. 이 연구에서는 100 MPa급 고강도 콘크리트 배합에 적합한 적층성을 확보하기 위하여 PTG를 변수로 한 3D 프린팅 실험을 수행하였으며, 이와 더불어 콘크리트 3D 프린팅 방법을 유사하게 모사할 수 있는 모의 적층실험 방법의 유효성을 검토하기 위하여 모의 적층실험을 수행하였다.

• 공업화학
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• 제27권4호
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• pp.366-370
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• 2016

본 연구에서는 1,3-bis(2-pyridyloxy)benzene과 $K_2PtCl_4$를 이용하여 백금을 중심으로 2개의 6원환을 이루는 백금 핀서 화합물을 합성하였다. NMR과 X-ray를 이용하여 구조를 규명하였고 백금원자를 중심으로 이루는 6원환 구조는 angle strain을 감소시켜 안정한 화합물을 형성하였다. 이 화합물을 알데하이드와 이민의 silylcyanation 반응에 적용하여 본 결과 수율 99%까지 나타내는 효과적인 촉매작용을 보여주었다.

• 대한기계학회논문집A
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• 제22권4호
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• pp.905-915
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• 1998

Static tensile tests using adhesive-bonded single-lap joints of aluminum alloy were conducted to investigate the effect of geometric factor, overlap length, adherend thickness, adhesive thickness and material composition of adherend/adhesive on the strength of adhesive joint. The average applied shear stress at joint fracture decreased with increasing lap length. However increasing the adherend thickness resulted in a higher joint strength. Higher yield strength of adherend and lower elastic modulus of adhesive is advantageous to the adhesive joint. Newly proposed modified joint factor could be well evaluated the influence of lap length, adherend thickness and adhesive thickness on the bond strength for adhesive joints.

• Journal of Microbiology and Biotechnology
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• 제10권6호
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• pp.753-758
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• 2000

Human leptin is identified as a 16kDa (146 amino acids) protein secreted from adipocytes which influences body weight homeostasis. In order to produce active leptin, the human obese gene coding for leptin was expressed in Bacillus subtilis WB600 strain which is deficient in six extracellular proteases. The recombinant leptin was produced in a culture supernatant, and in a culture supernatant, it was contained as high as 48% for total proteins. After simple purification steps, which consisted of ammonium sulfate precipitation and anion-exchange column chromatography, 2.3 mg of leptin with a purity greater than 95% was obtained from the 0.51 culture with the recovery yield of 38.3%. The purified leptin showed the correct folding structure with one disulfied bond.

• 대한안전경영과학회:학술대회논문집
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• 대한안전경영과학회 2006년도 춘계공동학술대회
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• pp.447-470
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• 2006

According to Granger causality test, yield of Cooperate Bond and export amount of Machinery have a meaning at statistical Confidence level of 10%. In case of index of the unit cost of export and export amount of Machinery, they have an interactive Granger cause. In yen dollar exchange rate and export amount of Machinery, former variable gives an unilateral Granger cause to latter that. Also, call rate gives an unilateral Granger cause to export amount of Machinery. In case of $M_3$ & export amount of Machinery, former variable have an influence on latter that at 5% Confidence level.

• 대한안전경영과학회:학술대회논문집
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• 대한안전경영과학회 2006년도 춘계공동학술대회
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• pp.471-484
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• 2006

The focus of analysis is effect on Non-electrical Machinery and Equipment of Macroeconomic variables through long-term and short-term periods. Also, this paper is related with implication on steady growth possibility of Non-electrical Machinery and Equipment. The period of variables is from 1985 to April in 2005. In case of not-available data is treated as missing figures. As spatial scope, these data are Non-electrical Machinery and Equipment on the basis of KSIC. In case of items, it composes MTI 1&3 digit of Korea International Trade Association (KITA), on the basis of HSK & classification of Korea Machinery industries. According to Granger causality test, yield of Cooperate Bond and export amount of Machinery have a meaning at statistical Confidence level of 10%. In case of index of the unit cost of export and export amount of Machinery, they have an interactive Granger cause. In yen dollar exchange rate and export amount of Machinery, former variable gives an unilateral Granger cause to latter that.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2004년도 춘계 학술발표회 제16권1호
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• pp.240-243
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• 2004

A new shear connection for the application of precast decks to PSC girders was proposed and push-off tests were conducted to evaluate the horizontal shear strength of the shear connection. Major parameters of the tests were connector type, shank area, vertical load, surface condition and bedding height. Judging from the test results, shear strength of the suggested shear connection was proportional to the shank area and yield strength of the connectors and was in inverse proportion to the bedding height. Shear connection with shear key at the surface showed better performance. An empirical equation for the evaluation of the shear strength of the shear connection without considering bond strength was proposed and it showed good correlation with the test results.

• 한국고분자학회:학술대회논문집
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• 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
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• pp.328-328
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• 2006

On mixing of 1,3-dehydroadamantane, 1, with electron-deficient vinyl monomers such as acrylonitrile and methyl acrylate in THF at r.t. in the absence of any initiator, the copolymerization spontaneously proceeded to give alternating copolymers in 28-88 % yield. By contrast, no reaction of 1 occurred, when isobutyl vinyl ether or styrene was mixed under the similar conditions. These contrastive results indicate the high electron density of a central s-bond in a strained [3.3.1]propellane derivative, 1. Alternating sequences of the resulting copolymers were characterized by NMR and MALDI-TOF-MS measurements. DSC and TGA measurements revealed the high thermal stability of the alternating copolymers containing bulky and stiff adamantane skeletons.