• Proceedings of the Korean Society of Sericultural Science Conference
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• 한국잠사학회 2002년도 Join Meetings of Korean Society of Sericultural Science and Japanse Society of Sericultural Science
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• pp.75-75
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• 2002

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• Microbiology and Biotechnology Letters
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• 제25권4호
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• pp.359-366
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• 1997

The usefulness of host range expanded recombinant viruses for economical viral insecticide and expression vector system has been studied. Host range expanded recombinant viruses, RecS-B6 and RecB-8, constructed by cotransfection of Bombyx mori nuclear polyhedrosis virus (BmNPV) and Autographa californica NPV (AcNPV), and a host range expanded AcNPV recombinant, Ac-BH, constructed by substitution of the 0.6Kb fragment of the BmNPV helicase gene were compared. The restriction enzyme digestion patterns showed that RecS-B6 and RecB-8 had expanded host ranges by genomic recombination and were more similar to genome of AcNPV than that of BmNPV. SDS-PAGE and PCR analysis showed that the polyhedrin gene of RecS-B6 and RecB-8 was derived from BmNPV genomic DNA. The morphology of polyhedra of recombinant viruses showed a slight difference between the two host cells, Sf and BmN cells, indicating that the morphology of polyhedra was influenced by host cells. The bioassay data for insect larvae showed that Ac-BH, compared to wild type viruses, had superior pathogenicity against Bombyx mori larvae but inferior pathogenicity against Spodoptera exigua larvae. Although the pathogenicity was lower than that of wild type viruses in both larvae, RecS-B6 showed the pathogenicity in both larvae. These results suggested that Ac-BH was a less useful economical insecticide than random genomic recombinant virus RecS-B6.

• Korean journal of applied entomology
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• 제36권3호
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• pp.264-269
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• 1997

Four strains of Bacillus thuringiensis were isolated froin the dead larvae of mulberry longicorn beetle (Apriong germari) and dung beetle (Aphodius apicalis). One nf four B. thuringiensis isolates turned out to be subspecies darinstadiensis but the remains were not identified using 33 B. thuringiensts flgellar ( H ) antibodies. Furthermore. bioassays of spore-parasporal inclusion protein mixture conducted against third instar larvae of A. gerrntrri or A. apicalis, second instar larvae of Bombyx mori, and third instar larvae of Cu1ex pipiens pullens showed that the isolates were non-toxic. To further confirm, four isolates were characterized and analysed by SDS-PAGE and agarose gel electrophoresis. The results revealed that parasporal protein and plasmid DNA patterns of four isolates are different from those of darmstadiensis and 20 known non-toxic B. thuringiensis strains, suggesting that the four isolates are novel non-toxic B. thuringiensis.

• Journal of Sericultural and Entomological Science
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• 제37권1호
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• pp.52-56
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• 1995

To investigate the effect of hemolymph of silkworm larvae on the multiplication of Bombyx mori nuclear polyhedrosis virus (BmNPV) in BmN-4 cells, BmN-4 cells were infected with BmNPV, which were sequentially Heated with the hemolymph exracted from B. mori larvae. When the culture media TC-100 containing 3% fetal bovine serum was mixed with 10% hemolymph heated at 65$^{\circ}C$ for 30 minutes, the released polyhedra by multiplication of BmNPV in BmN-4 cells were increased more than those of non-treated. However, multiplication of BmNPV in BmN-4 cells treated with non-heated hemolymph was not effective, since non-heated hemolymph was toxic for the cell growth. The result of plaque assay showed that plaque forming units in BmN-4 cells treated with heated hemolymph are significantly increased, suggesting that efficiency of multiplication of BmNPV in BmN-4 cells is due to increase not of cell growth but of infectivity of BmNPV.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권1호
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• pp.95-99
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• 2004

Synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG dinucleotides in particular base contexts are known to induce immunity in vertebrate cells. In insect, however, it was recent to find out that ODNs induces insect immunity as other immune inducer such as lipopolysaccharide. However, the finding was solely based on one lepidopteran insect, Bombyx mori, and the expression of insect immunity was neither dependent on numbers of CpG repeats nor methylation of CpG repeats within ODNs. Instead, foreignness of DNA has been suggested to be a key factor governing induction of antibacterial peptide. In this study, we expanded our previous understanding to the potentiality of ODNs as an immune inducer for antifungal peptide in Galleria mellonella and B. mori. To do this, a defensin-type antifungal peptide gene, reported from G. mellonella was cloned and partially sequenced from G. mellonella and B. mori successfully and utilized as a probe in the Northern blot analysis. We found out that ODNs also work as an immune inducer for antifungal peptide in the fat body and midgut of G. mellonella and B. mori larvae. Also, induction pattern of antifungal peptide was irrelevant to the numbers of CpG repeats within ODNs as previously reported on the induction pattern of antibacterial peptides.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권2호
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• pp.129-133
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• 2004

The breeding work was initiated by utilizing two Japanese hybrids namely C135${\times}$N134 and N137${\times}$C146 along with two evolved Indian breeds, J14 and A24. The breed CSR16 which is characterized with marked larvae and white dumbbell cocoons was evolved from the Japanese hybrid C135${\times}$N134 by crossing with J14, while the breed CSR17 which is characterized with plain larvae and white oval cocoons was evolved from the Japanese hybrid N137${\times}$C146 by crossing with A24. The hybrid of these evolved breeds i.e., CSR16${\times}$CSR17 has shown superior over control hybrid $KA{\times}{NB_4}{D_2}$ and on par with the ruling hybrid of CSR2${\times}$CSR4. The hybrid CSR16${\times}$CSR17 was authorized during 1999 by Central Silk Board, Bangalore, Government of India for commercial exploitation during favourable months based on national level race authorization test.

• International Journal of Industrial Entomology and Biomaterials
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• 제20권2호
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• pp.79-85
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• 2010

Eight bivoltine inbred lines of silkworm Bombyx mori L. viz., AP8, AP10, AP17 and AP18 with marked female and plain male larvae and AP1, AP54, AP71 and AP72 with all plain larvae synthesized from Chinese commercial hybrids were evaluated for their general combining ability (gca), specific combining ability (sca) and reciprocal effects for eight quantitative traits. The line AP18 was found to be best general combiner by virtue of significant positive and positive gca effects for seven out of eight traits studied followed by the lines AP10, AP8 and AP17. Seven hybrids recorded significant positive and positive sca effects for all the traits under study reflecting the interaction of alleles, additive and non additive gene action and dominant effects expressed by genes. Among the 28 reciprocal hybrids, AP54$\times$AP18 and AP1$\times$AP17 revealed significant reciprocal effects for the six traits followed by AP54$\times$AP8 for five and AP10$\times$AP71 for four traits.

• International Journal of Industrial Entomology and Biomaterials
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• 제23권1호
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• pp.137-141
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• 2011

A previous data was provided information for tissuespecific expression genes by means of whole-genome oligonucleotide microarray in the silkworm. We analyzed the tissue-specific expression patterns in the hemocyte tissue on 5 days of 5th instar larvae during the development of $B.$ $mori$. Total 5 candidates pick out from the $Bombyx$ $mori$ Microarray Database (BmMDB; http://silkworm.swu.edu.cn/microarray). To verify the hemocyte-specific expression, we analyzed by semi-quantitative and real-time quantitative RT-PCR using the highly expressed endogenous $Actin$ RNA as an intrinsic reference. In this study, we confirmed that one gene-sw17255- out of 5 candidates expressed in the hemocyte tissue, which was consistent with the previous data. Circulating hemocytes in the body fluid of the $B.$ $mori$ are most powerful target organ for producing biomaterials. We need further studies to find hemocyte-specific promoter region from sw17255 gene. Finally, this result can be applied in creating transgenic silkworms as a biomedical insect.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권1호
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• pp.31-35
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• 2001

Ten newly evolved bivoltine hybrids of silkworm (Bombyx mori L) were evaluated with control hybrid KA${\pm}$NB4D2 during three seasons of a year for their seasonal performance. Analysis of variance and other statistical methods were employed and the performance was observed in respect of 10 quantitative traits. The results showed significant genotype${\pm}$environment interaction with respect to four quantitative characters viz. fecundity, yield/10,000 larvae, filament length and raw silk (%). Environmental effects were significant for nine characters out of ten characters evaluated. A 105${\pm}$J2 and B${\pm}$NB4D2 were considered as highly adaptable hybrids to local conditions with high mean for maximum characters studied and found suitable to rear in all seasons.

• International Journal of Industrial Entomology and Biomaterials
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• 제6권1호
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• pp.75-79
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• 2003

Oral supplementation of zinc chloride to silkworm larvae in different concentrations (30, 60 and 90 ${\mu}g$/ml) to the fourth and fifth instar resulted in a significant increase in commercial traits, like larval weight, silk gland weight, cocooning percentage in lower concentrartion (30 ${\mu}g$) treated group. The female cocoon weight, shell weight and its ratio were significantly increased in 30 and 60 ${\mu}g$ treated groups, male cocoon weight, and shell weight in 30 ${\mu}g$ treated group and its ratio in 60 and 90 ${\mu}g$ treated groups, filament length, weight and moth emergence percentage in all the treated groups and egg productivity in 90 ${\mu}g$ treated group were increased significantly. The remaining groups did not show any significant changes compared with that of carrier control.