• Journal of Microbiology and Biotechnology
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• 제26권1호
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• pp.61-65
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• 2016

From the lees of bokbunja wine (LBW) made from Rubus coreanus Miquel, we have identified six compounds (1: trans-4-hydroxycinnamic acid; 2: trans-4-hydroxy-3-methoxycinnamic acid; 3: 3,4-dihydroxycinnamic acid; 4: 4-hydroxy-3-methoxybenzoic acid; 5: 3,5-dimethoxy-4-hydroxybenzoic acid; and 6: 3,5-dimethoxy-4-hydroxycinnamic acid (sinapic acid)) through silica gel chromatography and UHPLC-MS. The compounds 1-6 showed strong anticoagulation and platelet aggregation inhibitory activities without hemolytic effect against human red blood cells. To date, this is the first report of the in vitro anti-thrombosis activity of sinapic acid. Our results suggest that different cinnamic and benzoic acid derivatives are closely linked to the anti-thrombosis activity of LBW, and sinapic acid could be developed as a promising anti-thrombosis agent.

• 한국응용과학기술학회지
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• 제33권3호
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• pp.474-482
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• 2016

The black raspberry (Rubus coreanus Miquel) contains anthocyanin, tannins, gallotannin, gallic acid, ferulic acid and phenolics. It brightens the eyes and protects the liver and kidneys. It was effective for anti-aging. Thus, the purpose of this study was to inform the excellence of black raspberry and to screen antioxidant activity to ensure the possibility as a functional material. In this study, bioactive compounds in black raspberry were determined. Additionally, black raspberry was extracted by CM (chloroform:methanol, 2:1, v/v), 70% methanol and 70% ethanol, and were investigated and compared in vitro methods. Total phenol and flavonoid contents were measured to compare each different solvents. In addition, DPPH radical scavenging activity and ABTS radical scavenging activity, ferric reducing antioxidant power, reducing power were determined to measure the antioxidant activity. The results were the highest in 70% ethanol extracts, and the higher the concentration showed a significantly higher antioxidant capacity. When observed the relationship between the study, antioxidant activity of black raspberry was supposed to affect by the anthocyanin, phenol and flavonoid contents.

• 한국식품영양과학회지
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• 제40권6호
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• pp.839-847
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• 2011

본 연구는 한약탕제찌꺼기를 친환경 퇴비자원으로서 재활용하기 위하여 발효과정을 거친 퇴비(MHWC: medicinal herb waste compost)와 계분퇴비(PMC: poultry manure compost) 및 이를 혼합한 퇴비(MHWC+PMC, 1:1)를 식재2년생 복분자 포장에 0(UC: untreated control), 20, 40 Mg/ha로 처리하여 꽃이 첫 개화한 시기를 기점으로 15, 20, 25 DAF(day after flowering)를 수확시기로 하여 수확한 복분자 과실의 이화학적 특성을 조사하였다. 조단백질이 UC에 비해 모든 처리구가 증가하였고, 조지방은 UC와 비교하여 모든 수확시기에서 부분적으로 증가되는 경향이었다. 총당 함량은 UC에 비해 MHWC 처리구가 크게 증가하였다. 유리당 중 fructose와 glucose 함량은 MHWC 처리구가 다른 처리구보다 0.08~0.31, 0.08~0.18% 증가되었다. 유기산은 MHWC 및 PMC 처리로 인한 증가 경향을 보였고, 가장 높은 유기산은 citric acid로서 전체 유기산의 92.67~93.59% 수준이었다. 이상의 결과에서 MHWC 처리가 복분자의 당함량, 유리당 및 유기산의 함량을 증가시켜 품질향상에 효과가 있는 퇴비자원으로 생각되었다.

• 동아시아식생활학회지
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• 제25권2호
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• pp.287-299
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• 2015

Antioxidant activity of factory eel teriyaki sauce with bokbunja was investigated by functional analysis. Sugar content analysis of eel teriyaki sauce-free with bokbunja showed fructose and glucose but not maltose. Anthocyanin content of eel teriyaki sauce with bokbunja juice concentrate was 40%. DPPH radical scavenging activity and total polyphenol content of bokbunja added eel teriyaki sauce increased with increasing amount of bokbunja and were 80% higher than control sample. Total polyphenol content and DPPH radical scavenging activity were closely related to electron donating ability. The preferred concentrations of eel teriyaki sauce for commercial production were 60% for bokbunja, 40% for bokbunja concentrate, 20% for bokbunja powder eel.

• 한국식품저장유통학회지
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• 제20권4호
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• pp.532-538
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• 2013

복분자 추출물을 이용하여 과립차를 제조하고자 가공특성을 조사하였다. 복분자 추출물과 당 및 산을 적용하여 추출물 함량($X_1$, 0.4~1.2 g), 당 함량($X_2$, 6~10 g) 및 구연산 함량($X_3$, 0.1~0.3 g)의 비율로 혼합하여 과립에 관능적 특성 및 Hunter's color를 반응표면분석을 통하여 모니터링 하였다. 색상에 대한 가장 우수한 관능평점을 나타내는 배합비는 추출물 함량 0.96 g, 당 함량 7.05 g 및 구연산 함량 0.232 g이었다. 관능적으로 가장 우수한 향미를 나타내는 배합비는 추출물 함량 0.86 g, 당 함량 6.04 g 및 구연산 함량 0.215 g이었다. 관능적으로 가장 우수한 맛을 나타내는 배합비는 추출물 함량 0.92 g, 당 함량 6.39 g 및 구연산 함량 0.251 g이었다. 전반적인 기호도가 가장 높은 배합비는 추출물 함량 0.86 g, 당 함량 6.65 g 및 구연산 함량 0.272 g이었다. Hunter's color b값의 반응표면은 전반적인 기호도의 반응표면과 가장 유사한 것으로 나타났다. 따라서 복분자 과립차의 Hunter's color와 기호도는 Hunter's color a값 6.0, 복분자 추출물 함량 0.8 g 및 당 함량 0.6 g에서 선호되었다.

• 생명과학회지
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• 제25권7호
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• pp.757-764
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• 2015

복분자는 당도가 낮고 신맛이 강해 과실주로 주로 제조되고 있으며, 복분자주 제조 후 부생되는 주박은 대부분 폐기되고 있다. 본 연구에서는 현재까지 생리활성이 보고된 바 없는 복분자주 주박을 대상으로 열수 추출물, ethanol 추출물 및 이들의 다양한 분획물을 조제하여 항균, 항산화 및 항혈전 활성을 평가하였다. 그 결과, 복분자주 주박의 ethylacetate (EA) 분획은 413~459 mg/g의 높은 polyphenol을 함유하고 있으며, 그람양성세균에 대한 강력한 성장억제 활성과, DPPH 음이온, ABTS 양이온에 대한 우수한 radical 소거능, nitrite 소거능 및 환원력을 나타내었다. 또한 혈액내의 thrombin, prothrombin, coagulation factor 에 대한 저해를 통한 혈액응고저해 활성이 우수하였다. 복분자주 주박의 butanol 분획은 우수한 항산화력과 함께 내인성 혈전 생성에 관련된 aPTT 연장활성을 나타내었으며, aspirin에 필적하는 강력한 혈소판 응집저해 활성을 나타내었다. 한편 복분자주 주박의 열수 추출물의 순차적 유기용매 분획 후의 물 잔류물은 aspirin보다 강력한 혈소판 응집저해를 나타내어 새로운 항혈소판제 개발 소재로 이용 가능함을 확인하였다. 본 연구결과는 폐기되고 있는 복분자주 주박을 이용한 고부가가치 식품 및 향장소재 개발이 가능함을 제시하고 있다.

• 한국식품조리과학회지
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• 제32권4호
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• pp.458-465
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• 2016

Purpose: Bokbunja (Rub coreanus Miquel), mulberry and blueberry have been reported to have powerful anti-antioxidant and anti-aging activities. The purpose of this study was to compare the quality characteristics and antioxidant activities of gravy sauce added with Bokbunja, mulberry and blueberry. Methods: Quality characteristics pH, brix, color and sensory characteristics of samples were evaluated. Antioxidant activities of samples were compared using total phenol, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical scavenging and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities. Results: Mulberry juice showed significantly (p<0.05) higher values of pH and $^{\circ}Bx$ compared to Bokbunja and blueberry juice. Bokbunja juice also had significantly (p<0.05) higher value of Color-L value than mulberry and blueberry juice. The total phenol contents and ABTS cation radical scavenging activity of Bokbunja juice were 236.50 mgGAE/100 g and 61.66%, respectively, which were significantly (p<0.05) higher than those of blueberry and mulberry juice. pH value of gravy sauce added with mulberry (MBS) was 4.67, which was significantly higher than that of the control gravy sauce (GS) and gravy sauce added with Bokbunja (BJS) which had the lowest (p<0.05) pH value 4.33. $^{\circ}Bx$ of GS (13.50) was not significantly different from that of MBS and BBS. However, BJS showed the lowest (p<0.05) value $12.33^{\circ}Bx$. Total phenol contents and ABTS cation radical scavenging activity of BJS were 78.09 mgGAE/100 g and 14.47%, respectively, which were significantly (p<0.05) higher than those of MBS or BBS. Values of sensory characteristics color, sour aroma and sour flavor of BJS were significantly (p<0.05) higher value than those of other samples. Conclusion: Bokbunja juice and gravy sauce showed higher antioxidant activities and higher acceptance test compared to blueberry and mulberry. Thus, Bokbunja has the potential to be added to as high value products in the food industry.

• 한국식품저장유통학회지
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• 제16권3호
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• pp.400-404
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• 2009

복분자 부산물(박)을 유용한 천연 색소원으로 활용하기 위하여, 복분자 부산물(박)의 추출조건에 따른 색소 및 폴리페놀의 추출 특성에 대해 조사하였다. 용매 조건별 추출한 색소액의 총 흡광도는 80% 에탄올 용매에서 9.51 unit로 높은 편이었고, 폴리페놀은 에탄올 함량이 40%일 때 최고 수율이었고 에탄올 농도가 높아감에 따라 감소하는 경향이었다. 추출액의 총 흡광도는 용매에 citric acid 및 lactic acid를 첨가하였을 때 높았고, 폴리페놀의 함량은 ascorbic acid를 첨가한 용매 조건에서 높았다. 추출조건에서 부산물(박)의 혼합량이 많을수록 부산물(박) 단위 중량에 대한 총 흡광도 및 부산물(박) 단위 중량에 대한 폴리페놀 함량은 감소하는 경향이었으며, 2차례의 추출과정을 반복하였을 때 색소와 폴리페놀은 $85{\sim}90%$ 이상의 추출효율을 나타내었다.

• 한국미생물·생명공학회지
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• 제47권1호
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• pp.54-63
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• 2019

Rubus coreanus Miquel (RCM), also known as Korean blackberry or bokbunja, is used as a South Korean traditional medicine to treat acne and inflammatory skin conditions. The antimicrobial activity of RCM root and its active compounds remain unclear. In this study, we prepared a 50% ethanol fraction, ethyl acetate fraction, and acid-treated ethyl acetate fraction (aglycone fraction) of RCM root, and evaluated antibacterial activities against the skin pathogens Staphylococcus aureus, Pseudomonas acnes, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. In a paper disc assay, all fractions of RCM root showed antimicrobial activities against the five skin pathogens. The ethyl acetate fraction displayed 6-, 12-, and 2-fold higher minimal inhibitory concentration (MIC) than the 50% ethanol fraction against S. aureus, E. coli, and P. acnes, respectively. The aglycone fraction displayed 2-fold higher MIC than methyl paraben against P. acnes, S. aureus, E. coli, and P. aeruginosa. The ethyl acetate fraction displayed a minimal bactericidal concentration (MBC) similar to that of methyl paraben, and the aglycone fraction showed 2- to 4-fold higher MBCs than those of methyl paraben. In particular, the ethyl acetate fraction was not cytotoxic and showed thermal stability after incubation at high temperatures ($60-121^{\circ}C$). Finally, the ethyl acetate fraction was separated and four components were identified: procyanidin C, propelagonidin dimer, ellagic acid, and methyl ellagic acid acetyl pentose. The compounds showed high antibacterial activities. These results suggest that RCM root is potentially applicable as a natural preservative in cosmetics.

• 동아시아식생활학회지
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• 제22권5호
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• pp.634-641
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• 2012

In this study, the physicochemical and quality characteristics of eel sauce added with bokbunja powder were evaluated. Crude protein, crude fat, crude ash, and crude fiber contents increased in eel sauce with increasing amount of bokbunja powder. The lowest pH and salinity were observed in eel sauce added with 80% bokbunja powder, whereas the solid content, titratable acidity, and viscosity were the highest. Regarding Hunter's color results, lightness L and redness a values increased with increasing amounts of bokbunja, and the red color was significantly different between each sample. In the sensory evaluation results, the color, bokbunja flavor, bokbunja taste, salty taste, and moistness significantly increased with higher amount of bokbunja powder. Overall acceptability from 80%<60%<40%<0%<20% sequence supplemented with 20% in group symbols 7.08 to higher. Eel sauce added with 20% bokbunja powder produced the most desirable characteristics compared to other amounts of added bokbunja powder.