• Asian-Australasian Journal of Animal Sciences
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• 제21권5호
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• pp.617-623
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• 2008

A fine-mapping method exploiting linkage disequilibrium was used to detect quantitative trait loci (QTL) on the X chromosome affecting milk production, body conformation and productivity traits. The pedigree comprised 22 paternal half-sib families of Black-and-White Holstein bulls in the Netherlands in a grand-daughter design for a total of 955 sons. Twenty-five microsatellite markers were genotyped to construct a linkage map on the chromosome X spanning 170 Haldane cM with an average inter-marker distance of 7.1 cM. A covariance matrix including elements about identical-by-descent probabilities between haplotypes regarding QTL allele effects was incorporated into the animal model, and a restricted maximum-likelihood method was applied for the presence of QTL using the LDVCM program. Significance thresholds were obtained by permuting haplotypes to phenotypes and by using a false discovery rate procedure. Seven QTL responsible for conformation types (teat length, rump width, rear leg set, angularity and fore udder attachment), behavior (temperament) and a mixture of production and health (durable prestation) were detected at the suggestive level. Some QTL affecting teat length, rump width, durable prestation and rear leg set had small numbers of haplotype clusters, which may indicate good classification of alleles for causal genes or markers that are tightly associated with the causal mutation. However, higher maker density is required to better refine the QTL position and to better characterize functionally distinct haplotypes which will provide information to find causal genes for the traits.

• Animal Bioscience
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• 제34권1호
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• pp.127-133
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• 2021

Objective: This study was conducted to evaluate the correlation between body size traits, carcass traits, and primal cuts in Hanwoo steers. Methods: Sixty-one beef carcasses were classified for conformation and primal cut weight. Additionally, carcass weight, fat thickness, carcass dimensions, and longissimus muscle area were determined to complement the grading. Results: The average live weight and cold carcass weight were 759 and 469 kg, respectively. The mean carcass meat, fat, and bone proportions were 551, 298, and 151 g/kg, respectively. Primal cuts weights showed significant positive correlations (p<0.001) of 0.42 to 0.82 with live weight, carcass weight, and longissimus muscle area and a significant negative correlation with carcass fat (without shank, -0.38 to -0.10). Primal cut weights were positively correlated (p<0.01) with carcass length (0.41 to 0.77), forequarter length (0.33 to 0.57), 6th lumbar vertebrae-heel length (0.33 to 0.59), 7th cervical vertebrae carcass breadth (0.35 to 0.58), 5th to 6th thoracic vertebrae breadth (0.36 to 0.65), 7th to 8th thoracic vertebrae girth (0.38 to 0.63), and coxae girth (0.34 to 0.56) and non-significantly related to cervical vertebrae length and coxae thickness. Conclusion: There was a high correlation among live weight, carcass weight, longissimus muscle area, carcass length, 7th cervical vertebrae carcass breadth, 5th to 6th thoracic vertebrae breadth, and 7th to 8th thoracic vertebrae girth of the primal cuts yield. The correlation between fat and primal cut yields was highly significant and negative. Carcass length and 7th to 8th thoracic vertebrae girth, appear to be the most important traits affecting primal cut yields.

• Asian-Australasian Journal of Animal Sciences
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• 제14권4호
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• pp.447-454
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• 2001

For determining the relative genetic effects of Duroc (D) and Taoyuan (T) breeds on the economic traits of their hybrids, 72 litters of pigs, from four mating types, namely TT (T♂$\times$T♀), DD (D♂$\times$D♀) and D-T hybrids (TD, T♂$\times$D♀ and DT, D♂$\times$T♀) were used in this study. The various crossbreeding parameters were estimated by comparisons among mating types using linear contrasts of least-square analysis. The results of reproductive traits analysis showed that T breed had contributed superior genetic effects on the total number of piglets at birth (TBN) (p<0.10) and number of live piglets at 21 days (LP21) (p<0.05) to the D-T hybrids. Estimates of maternal genetic effects showed that the T females were superior in TBN (p<0.05), but inferior in average birth weight (ABW) and average litter birth weight (LBW) (p<0.01) to the D females. Direct heterosis effects were significant for LBW, LP21 and LWT21 (p<0.01). Least-squares analysis of other economic traits showed that T breed had relative negative effects on all growth traits, withers height (WH), body type index (BTI), average backfat thickness (ABF), carcass length (LENG), loin eye area (longissimus) (LEARA), and lean percentage (LEAN) of D-T hybrids (p<0.05). Estimates of direct genetic effects showed that the D breed was superior to the T breed in all growth and carcass traits except the average backfat (BF). Estimates of maternal genetic effects showed that average body weight at 180 days (WT180) of progenies from T sows were lighter than from D sows. Progenies from D females had larger and leaner carcass than those from T females. Direct heterosis effects were significant for average daily weight gains from 150 to 180 days ($ADG_{150-180}$) (p<0.05) and for average body weights at 150 (WT150), and 180 days (WT180), average daily weight gains from birth to 150 and 180 days ($ADG_{150}$ and $ADG_{180}$, respectively), WH, body length (BL), ABF, BTI, and LENG (p<0.01). The results showed that D-T hybrids tended to have superior TBN and LP21 than D breed, and to be superior in all growth and most conformation and carcass traits to the T breed.

• 농업생명과학연구
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• 제51권6호
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• pp.89-105
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• 2017

본 연구는 부모의 혈통정보를 모두 아는 혈통 및 고등등록우로 등록된 한우 암소 25,516두의 선형 및 외모심사형질에 대해 유전모수를 추정하였다. 각 심사형질에 영향을 주는 것으로 판단되는 주요인을 선정하여 다형질 개체모형에 적용하여 EM-REML 알고리즘 분석방법을 통해 각 형질에 대한 유전력과 유전상관과 표현형상관을 추정하였다. 한우 암소의 17개 선형심사형질 및 10개 외모심사형질(종합점수 포함)의 유전력 추정치의 범위는 0.03(유두배열)에서 0.42(체장)까지로 추정되었다. 체고, 체장, 강건성, 체심, 윤곽성, 정강이두께, 피모의 색, 엉덩이기울기, 고장, 좌골폭, 넓적다리의 두께, 유방용적, 유두길이, 유두배열, 발굽기울기, 뒷다리 비절기울기, 뒤에서 본 뒷다리자세, 체적 균형, 자질 품위, 머리 목, 전구, 중구, 엉덩이, 넓적다리, 유기, 지제보양 및 종합점수의 유전력은 각각 0.4, 0.42, 0.27, 0.25, 0.06, 0.14, 0.22, 0.31, 0.19, 0.17, 0.29, 0.04, 0.07, 0.02, 0.11, 0.03, 0.16, 0.27, 0.08, 0.15, 0.14, 0.14, 0.19, 0.16, 0.05, 0.08 및 0.3으로 추정되었다. 한편, 다형질개체모형에 의해 추정된 17개 선형심사형질과 종합점수에 대한 유전상관추정 결과는 다음과 같다. 고장과 좌골폭간에 0.96의 가장 강한 양(+)의 유전적 관계를 나타내었다. 반면 발굽기울기와 뒷다리 비절기울기간에는 -0.57의 가장 큰 음(-)의 유전상관을 보였다. 전체외모 형질인 체고, 체장, 간겅성 및 체심은 각 형질간에, 그리고 기타 형질들과 강한 양(+)의 유전상관관계를 나타내었다. 특히 체고 및 체심과 고장 및 좌골폭간에 높은 양(+)의 상관관계를 나타내었으며, 체고와 엉덩이기울기간에는 0.32의 양(+)의 상관을 나타내었다.

• Asian-Australasian Journal of Animal Sciences
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• 제26권7호
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• pp.911-915
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• 2013

The aim of this study was to investigate allele frequencies at the CSN1S2 locus in two Chinese dairy goat breeds and the effects of its variation on dairy goat economic traits. Seven hundred and eight goats from Xinong Saanen (XS, n = 268) and Guanzhong (GZ, N = 440) breeds were selected. The milk samples of 268 XS goats were collected during the middle of lactation, body size parameters (708 goats) and daily milk yield (202 goats) were registered. The RFLP (restriction fragment length polymorphism) and SSCP (single strand conformation polymorphism) were used to detect the polymorphisms in CSN1S2. The Hardy-Weinberg (HW) equilibrium and the associations between body size, milk yield and composition and the genotypes were calculated. The results revealed that only A and F CSN1S2 alleles were found in the two Chinese dairy goat breeds. Allelic frequencies of A and F were 0.795, 0.205 and 0.739, 0.261 in Xinong Saanen and Guanzhong population respectively. Xinong Saanen breed was in Hardy-Weinberg equilibrium, while Guanzhong breed deviated from Hardy-Weinberg equilibrium (p<0.05). The association of polymorphism with economic traits indicated that the goats with FF genotype have higher milk fat and total solid concentration than those with AA and AF genotypes (p<0.05).

• Asian-Australasian Journal of Animal Sciences
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• 제18권9호
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• pp.1221-1225
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• 2005

The peroxisome proliferator-activated receptors (PPARs) are members of a superfamily of nuclear hormone receptors. Lots of studies in rodents and humans have shown that PPARs were involved in lipid metabolism and adipocyte differentiation. The main objective of this work was to detect the single nucleotide polymorphisms (SNPs) in whole coding regions of peroxisome proliferator-activated receptor alpha (PPAR-$\alpha$) and gamma (PPAR-$\gamma$) genes with approach of single strand conformation polymorphism (SSCP) in the chicken population of Arber Acres broiler, Hyline layer and three Chinese native breeds (Shiqiza, Beijing You, Bai'r). Two SNPs of C1029T and C297T were found in chicken PPAR-$\alpha$ and PPAR-$\gamma$ genes respectively and each SNP found three genotypes in the experimental populations. The results showed that the distribution frequency of 3 genotypes in Arber Acres broiler, Hyline layer and Chinese native breeds had significant differences on the PPAR-$\alpha$ and PPAR-$\gamma$ gene respectively (p<0.01). Furthermore, in the PPAR-$\alpha$ gene, the results of least square estimation for genotypes and body composition traits showed the BB genotype birds had higher abdominal fat weight (AFW) and percentage of abdominal fat (AFP) than AA genotype birds (p<0.05). From these we conjecture the PPAR-$\alpha$ and PPAR-$\gamma$ genes were suffered intensive selection during the long term commercial breeding and the PPAR-$\alpha$ gene may be a major gene or linked to the major genes that impact chicken fat metabolism and the SNPs could be used in molecular assistant selection (MAS) as a genetic marker for the chicken fatness traits.

• Asian-Australasian Journal of Animal Sciences
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• 제27권8호
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• pp.1075-1081
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• 2014

CRBP1 (cellular retinol binding protein 1) and CRBP3 (cellular retinol binding protein 3), are important components of the retinoid signaling pathway and take part in vitamin A absorption, transport and metabolism. Based on the role of vitamin A in chicken laying performance, we investigated the polymorphism of CRBP1 and CRBP3 genes in 349 chickens using single strand conformation polymorphism and DNA sequencing methods. Only one polymorphism was identified in the third intron of CRBP1, two polymorphisms were detected in CRBP3; they were located in the second intron and the third intron respectively. The association studies between these three SNPs and laying performance traits were performed in Erlang mountainous chicken. Notably, the SNP g.14604G>T of CRBP1 was shown to be significantly associated with body weight at first egg (BWFE), age at first egg (AFE), weight at first egg (WFE) and total number of eggs with 300 age (EN). The CRBP3 polymorphism g.934C>G was associated with AFE, and the g.1324A>G was associated with AFE and BWFE, but none of these polymorphisms were associated with egg quality traits. Haplotype combinations constructed on these two SNPs of CRBP3 gene were associated with BWFE and AFE. In particular, diplotype H2H2 had positive effect on AFE, BWFE, EN, and average egg-laying interval. We herein describe for the first time basic research on the polymorphism of chicken CRBP1 and CRBP3 genes that is predictive of genetic potential for laying performance in chicken.

• Asian-Australasian Journal of Animal Sciences
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• 제31권6호
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• pp.775-783
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• 2018

Objective: The purpose of this study was to investigate the genetic effects of six keratin (KRT) genes on the wool traits of 418 Chinese Merino (Xinjiang type) (CMXT) individuals. Methods: To explore the effects and association of six KRT genes on sheep wool traits, The polymerase chain reaction-based single-strand conformation polymorphism (PCR-SSCP), DNA sequencing, and the gene pyramiding effect methods were used. Results: We report 20 mutation sites (single-nucleotide polymorphisms) within the six KRT genes, in which twelve induced silent mutations; five induced missense mutations and resulted in $Ile{\rightarrow}Thr$, $Glu{\rightarrow}Asp$, $Gly{\rightarrow}Ala$, $Ala{\rightarrow}Ser$, $Se{\rightarrow}His$; two were nonsense mutations and one was a same-sense mutation. Association analysis showed that two genotypes of the KRT31 gene were significantly associated with fiber diameter (p<0.05); three genotypes of the KRT36 gene were significantly associated with wool fineness score and fiber diameter (p<0.05), three genotypes of the KRT38 gene were significantly associated with the number of crimps (p<0.05); and three genotypes of the KRT85 gene were significantly associated with wool crimps score, body size, and fiber diameter (p<0.05). Analysis of the gene pyramiding effect between the different genotypes of the gene loci KRT36, KRT38, and KRT85, each genotype in a gene locus was combined with all the genotypes of another two gene loci and formed the different three loci combinations, indicated a total of 26 types of possible combined genotypes in the analyzed population. Compared with the other combined genotypes, the combinations CC-GG-II, CC-HH-IJ, CC-HH-JJ, DD-HH-JJ, CC-GH-IJ, and CC-GH-JJ at gene loci KRT36, KRT38, and KRT85, respectively, had a greater effect on wool traits (p<0.05). Conclusion: Our results indicate that the mutation loci of KRT31, KRT36, KRT38, and KRT85 genes, as well as the combinations at gene loci KRT36, KRT38, and KRT85 in CMXT have significant effects on wool traits, suggesting that these genes are important candidate genes for wool traits, which will contribute to sheep breeding and provide a molecular basis for improved wool quality in sheep.

• 생명과학회지
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• 제28권7호
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• pp.795-801
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• 2018

본 연구는 제주마 체중 형질과 말의 3번 및 9번 염색체 단일염기변이마커간의 관련성을 분석하기 위해 수행되었다. 본 연구에 사용된 DNA 샘플은 제주특별자치도 축산진흥원에서 등록관리 중인 제주마 320두를 제공받았고, 생체중 자료는 한국마사회에서 시행한 경마에 출주한 제주마의 체중자료를 이용하였다. 기존에 말의 체형관련형질과의관련성이 보고된 바 있는 말의 3번 염색체상에 위치한 BIEC2-808466, BIEC2-808543, BIEC2-808967, BIEC2-809370 단일염기변이마커와 9번 염색체상에 위치한 BIEC2-1105370, BIEC2-1105372, BIEC2-1105377, BIEC2-1105505 and BIEC2-1105840 유전자형을 분석하였다. 관련성 분석을 위한 종속변수는 선형혼합모형을 이용해서 추정한 생체중 육종가를 이용하였다. 관련성 분석결과 6개의 마커(BIEC2-808543, BIEC2-808967, BIEC2-809370, BIEC2-1105370, BIEC2-1105372, and BIEC2-1105377)와 생체중 추정육종가간의 유의성이 인정되었다. 또한, BIEC2-808967과 BIEC2-1105377의 유전자형조합을 구성하여 생체중 추정육종가에 대한 효과를 분석한 결과, 두 마커의 유전자 조합은 생체중 변이에 유의한 효과가 있었다. 본 연구에서 유의성이 인정된 단일염기변이마커들은 제주마의 체중 개량을 위한 선발육종체계의 효율성을 높일 수 있는데 사용될 수 있을 것으로 사료되지만, 이 들 마커효과의 좀더 정확한 추정을 위한 추가의 연구가 필요할 것으로 생각된다.

• Journal of Animal Science and Technology
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• 제47권2호
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• pp.159-166
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• 2005

Porcine myostatin(MS1N) gene plays a key role in the differentiation of myoblast and muscle development. Genetic polymorphism was screened by single stranded conformation polymorphism(SSCP) analysis and subsequent DNA sequencing detected a nucleotide substitution(C2150T) in exon 3 of MSIN gene. Phenotypic association of the polymorphism was tested in a Landrace population and positive effects of the allele T for lean growth traits were found in the population. Even though it is not significant, the pigs have IT and TC genotypes were heavier for the body weight at birth and at twenty weeks of age than those containing genotype. Cc. However, the allele T was significantly associated with higher eye muscle area(P < 0.05). As a result of this study, we suggested that the allele T in exon 3 of MSTN gene comes a significant effect for increasing the eye muscle area without decreasing backfat thickness. This polymorphism did not change the amino acid but Taq I -RFLP matched to SSCP band patterns in exon 3 of MSTN gene, which will be an useful molecular marker for breeding of Landrace pigs.