• Journal of Microbiology and Biotechnology
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• 제26권6호
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• pp.1046-1056
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• 2016

RV-23 is a melittin-related antibacterial peptide (MRP) with lower cytotoxicity than either melittin or AR-23, another MRP. The aim of this study was to explore the mechanism of RV-23's antibacterial selectivity and its hemocompatibility. The results showed that all the peptides exhibited lytic activity against Staphylococcus aureus and Escherichia coli, with RV-23 showing the highest potency. Moreover, RV-23 had lower cytotoxicity than melittin or AR-23 at their minimal inhibitory concentration. In addition, CD experiments showed that melittin, RV-23, and AR-23 all had a typical α-helical structure, and RV-23 had the lowest α-helix content. The structural information showed that RV-23 has the lowest hydrophobicity and highest hydrophobic moment. Because hydrophobicity and α-helix content are believed to correlate with hemolysis, the results indicate that the selective lytic activity against bacteria of RV-23 may be due to its low hydrophobicity and α-helicity, which lead to low cytotoxicity without affecting antibacterial activity. Furthermore, RV-23 did not affect the structure and function of blood components such as red blood cells, platelets, albumin, and the blood coagulation system. In conclusion, RV-23 is a cell-selective antibacterial peptide with high hemocompatibility due to its unique structure.

• 대한한의학방제학회지
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• 제26권1호
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• pp.81-102
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• 2018

Objectives : I analysed daehwangmokdan-tang's compatibility principle by the system of chief, deputy, assistant, and envoy and investigated pharmacological activities by categorizing with chemical components, molecular level, cellular level, animal level and human level based on Korean and Chinese studies for this formula. Methods : Daehwangmokdan-tang's compatibiltity principle was examined by the system of chief, deputy, assistant, and envoy. I looked into studies that presented in Korea from 1956 to 2016 about daehwangmokdan-tang through KOREA INSTITUTE OF ORIENTAL MEDICINE, Korean medicine information system (OASIS) and in Chinese for 20 years about daehwangmokdan-tang through China National Knowledge Infrastructure, CNKI. Then classify into chemical components, molecular level, cellular level, animal level and human level to analyse. Results : According to the system of chief, deputy, assistant, and envoy, chief herb is Rhei Radix et Rhizoma and Moutan Cortex, deputy herb is Natrii Sulfas and Persicae Semen, assistant and envoy herbs are Trichosanthis Semen. The amount of extraction of paeonol, total anthraquinone, and conjugated anthraquinone from daehwangmokdan-tang with the formulation of the system of chief, deputy, assistant, and envoy was the highest, and in the formulation of chief herb and deputy herb, the extraction amount of paeonol and conjugated anthraquinone was the lowest. With other formulations, the amount of extraction of total anthraquinone and conjugated anthraquinone was improved, although the degree was different. In particular, when it is blended with Persicae Semen as a deputy herb, the extraction amount of total anthraquinone and conjugated anthraquinone of Rhei Radix et Rhizoma as a chief herb is greatly increased, and the extraction amount of paeonol is rather different, but it is lowered. It was found that the amount of Mg Ca K Na in daehwangmokdan-tang was the highest. Pharmacological activities can be detected in inflammatory mediators and enzymes for molecular level. For cellular level, it can be determined in lipopolysaccharide (LPS)-stimulated RAW 264.7 cell line. In mouse and rats for animal level and human level, in inflammatory diseases (acute appendicitis, acute pancreatitis, acute cholecystitis, acute abdominal disease, ect.), pharmacological activities was caught. Conclusions : From the above results, daehwangmokdan-tang is composed in line with the system of chief, deputy, assistant, and envoy, suggesting that there is certain rationality and scientific. Pharmacological activities of daehwangmokdan-tang are effective to anti-inflammation, improvement of sepsis, analgesic, muscle relaxation, and improvement of intestinal flora and its metabolites. Daehwangmokdan-tang is consistent with the action of diuresis and anti-inflammation in terms of the content of elements, suggesting that there is action of purging fire and removing blood stasis, defecation detoxification, cooling blood and clearing heat and activating blood and dispersing stasis.

• 한국진공학회지
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• 제6권S1호
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• pp.1-15
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• 1997

Titanium oxide films were prepared by ion beam enhanced deposition where the films were synthesized by deposition titianium atoms and simultaneously bombarding with xenon ion beam at an energy of 40 keV in an $O_2$ environ,ent. Structure and composition of titanium oxide films were investigated by X-ray Doffractopm (XRD) Ritjerfprd Backscattering Spectroscopy (RBS) and X-ray Diffraction(XRD) Rutherford Backscattering Spectroscopy (RBS) and X-ray photoelectron spectroscopy (XPS) The results show that thestructure of the prepared films exhibit a rutile phase structure wit high(200) orientation and the O/Ti ratio of the titanium oxide films was about 2:1 XPS anlysis shows that $Ti^{2+},Ti^{3+}\;and\;Ti^{4+}$ chemical states exist on the titanium oxide films. the blood compatibility of the titanium oxide films was studied by measurements of blood clotting time and platelet adhesion. The results show that the anticoagulation property of titanium oxide films improved significantly and better than that of LTI-carbon which was widely used to fabricate artificial heart valve.

• 대한한의학방제학회지
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• 제15권2호
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• pp.1-8
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• 2007

The source of Gwibitang(歸脾湯) is the book of Jesaebang(濟生方), The formulae is excluded Angelicae gigantis radix(當歸) and Polygalae radis(遠志) in the Jesaebang(濟生方), Jn the Guch iryuyo(口齒類要), Jeongcheryuyo(正體類要) and Gyojubuinyangbang(校註婦人良方), has been bec ame White Pria cocos Wolf(白茯笭) instead of Hoelen cum Pini Radix(白茯神), and is followed constitution of Uihagypmun(醫學入門) in the Donguibogam(東醫寶鑑). Basic formulae of Gwibitang(歸脾湯) consist of Sanjointang(酸棗仁湯), Sagunjatang(四君子湯). Hwanggitang, Danggwibohyeoltang(當歸補血湯), a kind of Jeongjihwan(定志丸類), a kind of Chongmyeongtang(聰明湯類). To deficiency of the heart and spleen, apply Sanjointang(酸棗 仁場), a kind of Jeongjihwan(定志丸), Sagunjatang(四君子湯) and Hwanggitang. To insufficiency of the spleen-gi(脾氣), apply Sagunjatang(四君子湯), Hwanggitang and a kind of Jeonssiigongsan(定志小丸). To deficiency of blood, apply Danggwibohyeoltang(當歸補血湯). Gwibitang(歸脾湯) is used for a mnesia and severe palpitation as a result of deficiency of the heart and spleen and deficiency of both gi(氣) and blood(血).

• 셀메드
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• 제5권4호
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• pp.27.1-27.6
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• 2015

Literatures and experimental studies have shown that Prunella has an effect on anti-hypertension, however, its components are complicated, so that it is still difficult to clear the specific roles of its various components in blood pressure regulation in. So we decide to systematically study the anti-hypertension mechanism of Prunella. We integrated multiple databases and constructed molecular interaction network between the chemical constituents of Prunella Vulgaris and hypertension based on entity grammar systems model. The network has 262 nodes and 802 edges. Then we infer the interactions between chemical compositions and disease targets to clarify the anti-hypertension mechanism. Finally, we found Prunella could influence hypertension by regulating apoptosis, cell proliferation, blood vessel development and vasoconstriction, etc. Thus this study provides reference for drug development and compatibility, and also gives guidance for health care at a certain extent.

• 자동차안전학회지
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• 제7권4호
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• pp.9-15
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• 2015

The relationship between BAC(Blood Alcohol Concentrations) and BrACs(Breath Alcohol Concentrations) and also partition ratio of healthy Korean adult males (96 males) are researched in this paper and its dependency is described according to TBW (total body water), BMI (body mass index), BFM (body fat mass), and PBF (percentage of body fat). Among the above four variables, TBW affects significantly to the partition ratio compared to the other variables. The partition ratio of Korean healthy males showed 1,913 (95 % Confidence Interval (C.I.) from 1,889 to 1,937) for the whole time intervals. However, when Q was averaged after 60 minutes later, its values was 2,011 (95 % C.I. from 1,982 to 2,040). Bland-Altman plots showed the compatibility of measurement methods of multi-gas analyzer, and the biases according to the partition ratios (Q=2,100 and Q=1,913) gave -0.0052 % (95 % CI from -0.0059 to -0.0045%) and -0.0004 % (95 % CI from -0.0011 to +0.0003%), respectively.

• 대한의용생체공학회:의공학회지
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• 제18권2호
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• pp.147-156
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• 1997

Various prosthetic heart valves have been developed and used clinically, but they have problems, such as thrombogenecity, hemoltsis, high cost and low durability. New types of trileaflet polymer heart valves have been developed in order to use them as inlet and outlet valves in a ventricular assist device. The aim of this study is to determine the hydrodynamic effectiveness of the newly designed trileaflet polymer valves and their feasibility for temporary use in the blood pumps. Trileaflet polymer valves are made of polyurethane, because of its good blood compatibility, high tonsil strength and good resistance to fatigue. An in vitro experimental investigation was perf'ormed in order to ev91ua1e hydrodynamic performance of the trileaflet polymer valves having different design and fabrication tech- niques. The St. Jude Medical valve (SJMV) and floating-type monoleaflet polymer valve (MLPV) were also tested The pressure drop across the valve, leakage volume, and the flow patterns mere investigated for valves. The result of comparative tests showed that the trileaflet polymer valves had a better hydrodynamic performance than the others. TPV which has two stable membrane shape showed the lowest back flow. The pressure hops of TPVs were lower than that of MLPV, but slightly higher than SJMV. The hydrodynamic performance of valves under the pulastile flow showed the similar results as steady flow. The velocity profiles and turbulent intensities were measured at the distal sites of valves using a hot-film anemometer. Central flow was maintained in trileaflet polymer valves, and the maximum turbulent intensities were lower in TPVs comparing to MLPV.

• Archives of Plastic Surgery
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• 제38권4호
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• pp.369-375
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• 2011

Purpose: Many hemostatic agents and dressings have been tested with variable degree of success. Chitosan has a positive charge, it attracts red blood cells, which have a negative charge. Our goal is to test the efficacy of new developed chitosan-based hemostatic materials in providing durable hemostasis in a high-flow arterial wound model. Methods: We compared each group with SD rats motality tests and in vitro blood compatibility test by blood clotting index (BCI). We devided the SD rats into 6 groups (N =15) by type of hemostatic agents. A: 100% nonwoven chitosan (degree of the deacetylation: 90%). B: 50% N-acetylation on nonwoven of chitosan gel (degree of the deacetylation: 50%). C: 60% N-acetylation on nonwoven of chitosan ge (degree of the deacetylation: 40%)l. D: Cutanplast$^{(R)}$. E: HemCon$^{(R)}$ F: Gauze. In vivo test, a proximal arterial injury was created in unilateral femoral arteries of 90 anesthetized SD rats. Each materials was made same size and thickness then applied to the injury site for 3 minutes. In vitro test, we compared each group with BCI in human blood. Results: In vivo test, group A showed lower motality rate of 46% than any other groups, Group B and C showed lower motality rate of 60% than group D and E's motality rate of 66%. In vitro test, BCI of group A ($30.6{\pm}1.2$) and B ($29.3{\pm}1.0$) were showed nearly about group D ($29.1{\pm}1.8$) and E ($27.4{\pm}1.6$). Group C ($37.1{\pm}2.0$) showed higher BCI than group A and B, it means group C decreased blood clotting. Conclusion: In conclusion, this study suggests a newly developed chitosan-based hemostatic materials induced durable hemostasis and increased blood clotting, and are considered as effective biologic hemostatic agents.

• 폴리머
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• 제25권3호
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• pp.343-348
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• 2001

Perfluorinated polyether diol(Fomblin ZDOL$ZDOL^{(R)}$)과 4,4'-diphenyl methane diisocyanate(MDI)로부터 합성된 불소화된 폴리우레탄 탄성체의 기계적 성질과 혈소판 점착 특성을 연구하였다. Fomblin 함량과 혼합 사용된 polyether polyol의 종류에 따른 기계적 성질의 검토를 인장실험 및 dynamic mechanical analysis를 통해 행하였다. 또한 혈소판 점착실험을 통해 불소화된 폴리우레탄 탄성체의 혈액적합성을 평가하였다. 기계적 물성은 혼합된 polyether polyol의 종류와 함량에 영향을 받았고, 혈소판 점착은 모든 불소화된 폴리우레탄에서 불소화 함량의 증가에 따라 감소하였다.

• 한국염색가공학회지
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• 제9권5호
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• pp.52-62
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• 1997

Abstract Polyurethanes have been designed and fabricated into membranes with unique separation properties. Moreover, polyurethane microcapsules also have been reported actively as controlled release materials for their excellent blood compatibility, tensile strength and permeability. In this study, polyurethane microcapsules were synthesized by interfacial polymerization in an aqueous poly(ethylene glycol) dispersion of toluene diisocyanate in perfume oil using poly(vinyl alcohol) as the stabilizing agent. The effect of a few important process conditions on the average particle size and distributions, morphologies, and thermal properties to design microcapsules for the sustained release system was investigated.