• The Journal of the Korean Society for Microbiology
• /
• v.18 no.1
• /
• pp.59-65
• /
• 1983

To investigate the effects of placental serum to in vitro culture of the normal human lymphocytes the peripheral blood lymphocytes were isolated by ficoll-hypaque separation method in 20 healthy human adults. The blast transformation respone of lymphocytes to mitogens were observed by stimulation with PHA($25{\mu}g/ml$) and Con A($25{\mu}g/ml$) using RPMI 1640 media containing 20% placental serum(PS), tetal calf serum(FCS), or humans AB serum(AB). And one-way mixed lymphocyte culture test was performed between these unrelated person compounded into stimulators and responders to investigate the effect of placental serum. The following results were obtained. 1. In 20 experimental cases, these were no significant diffenence between FCS, AB, and PS in untreated control cultures. 2. In PHA-treated cultures, whereas the blast transformation rate of the FCS groups and AB groups were $40.8{\pm}4.3%$ and $44.6{\pm}4.3%$ respectively, that of PS groups were $21.7{\pm}3.4%$, Similar results were obtained in Con A-meated cultures. Therefore, placental serum inhibited the mitogenic response of lymphocytes significantly. 3. In MLC tests, stimulation index of the FCS groups and AB groups were $18.5{\pm}3.5$ and $20.1{\pm}3.3$ respectively. But placental serum inhibited MLC response of lymphocytes significantly($7.4{\pm}1.9$).

• Korean Journal of Veterinary Research
• /
• v.35 no.4
• /
• pp.769-776
• /
• 1995

This study was undertaken to develop the monoclonal antibody(MAb) for lymphocytes of Korean native cattle by the cell hybridization of myeloma P3/NS-1/ 1-Ag-4-1 and spleen cells from BALB/c mice hyperimmunized with nylon wool column eluted peripheral T lymphocytes of Korean native cattle. The isotype of MAb KCT-14 against T lymphocyte was mouse $IgG_1$. KCT-14 positivity of mononuclear cells(MNC) from peripheral blood lymphocytes, nylon wool nonadherent and adherent-lymphocytes was 41.7%, 58.4% and 22.6%, respectively. And that of mesenteric lymph node-, spleen and thymus-MNC was 43.3%, 40.2% and 33.6%, respectively. Immunoperoxidase staining of frozen tissue sections showed that the MAb positive cells were located in the medulla of the thymus and in the paracortical area and the mantle zone of the germinal center in the lymph nodes. These results indicated that KCT-14 was one of the MAb for investigate of T lymphocyte subpopulations in the Korean native cattle.

• Asian-Australasian Journal of Animal Sciences
• /
• v.12 no.7
• /
• pp.1031-1034
• /
• 1999

Circulating lymphocytes collected from control and heat-stressed buffaloes were subjected to in vitro culture with glucocorticoids, epinephrine or serotonin and their effect, if any, on the release of intracellular prolactin (PRL) was studied using ELISA and C-ELISA techniques. It was noted from the study that PRL level was higher in lymphocytes than in plasma of the control and heat-stressed animals, and that the PRL levels increased in the plasma of heat-stressed animals compared to that of non stressed animals with a significant decrease in lymphocytic PRL content by heat stress. Epinephrine and serotonin significantly increased the release of intracellular PRL from the lymphocytes of both in the control and the heat-stressed buffaloes but release of PRL from lymphocyte was not significantly changed by cortisol treatment in both control and heat-stressed buffaloes as compared to epinephrine and serotonin in vitro. When lympocytes were incubated with serotonin, it caused drastic lysis of the lymphocytes but epinephirine and cortisol did not show any lysis. It may be concluded from this study that hormones like epinephrine or serotonin known to increase during stress, release intracellular PRL from lymphocytes, the satellite PRL storage/synthesizing organ of blood, although the mechanism of the release is different.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.9
• /
• pp.5257-5261
• /
• 2013

The micronucleus frequency (MNF) in peripheral blood lymphocytes (PBL) is a biomarker of chromosomal damage and genome instability in human populations.The relationship of micronucleus frequency with prognosis of patients with acute leukemia is not clear. We therefore investigated MNF in mitogen-activated peripheral blood lymphocytes from patients with hematologic diseases and solid tumours. Patients included 50 with acute leukemia, 49 diagnosed with myelodysplastic syndrome (MDS), 54 with benign blood diseases, and 45 with solid tumours, examined with 50 healthy controls. The mean MNF was significantly higher in cases of hematologic diseases and solid tumor patients than in healthy controls (P<0.001). There was no evident difference between MNF in the acute leukemia ($7.15{\pm}2.18$) and solid tumor groups ($7.11{\pm}1.47$), but both were higher than in the MDS group ($5.12{\pm}1.29$) and benign blood diseases group ($3.08{\pm}1.08$). Taking 7.15‰, the average MNF of the acute leukemia group as standard, and dividing 50 cases of acute leukemia patients into high MNF group ($MNF{\geq}7.15$‰) and low MNF group (MNF<7.15‰). The overall response (complete remission + partial remission) rates of the low MNF group were significantly higher than in the high MNF group (P=0.001). The high MNF group further showed lower overall survival rates than the low MNF group. MNF expression and progression-free survival seemed to have a opposite relationship, with a correlation coefficient of -0.702. These data indicate that MNF in peripheral blood lymphocytes is important for evaluation of prognosis of acute leukemia patients, and it can reflect progression of disease to a certain degree.

• Analytical Science and Technology
• /
• v.27 no.5
• /
• pp.248-253
• /
• 2014

This study aimed to evaluate the effects of exposure to manganese (Mn) and lead (Pb) on immune system. The subjects were 42 male workers, among whom 13 office workers (Group I) had never been occupationally exposed to heavy metals, 21 were worked in manufacturing factories (Group II) and 8 were welders (Group III). The mean blood Mn and Pb level by groups were significantly different. The numbers of CD19+ and total lymphocytes in Group I were significantly higher than those in other groups, but no significant differences were found in other T lymphocytes subpopulation. Mn and Pb concentrations showed negative correlation with T lymphocytes subpopulation, but Mn concentrations were statistical significances with T lymphocytes subpoplation except CD4+CD45RO+ and natural killer cell. Pb concentration was only statistical significance with total lymphocytes. Our results suggest that occupationally exposed to Mn and Pb can affect the cellular immune response.

• Korean Journal of Veterinary Research
• /
• v.28 no.1
• /
• pp.49-58
• /
• 1988

To investigate the effects of T-2 toxin on the blastogenesis of lymphocytes, pathology, hemogram and blood chemistry in the goat, the korean native goats were treated orally with T-2 toxin for 21 days with a dosage of 0.6mg per kg body weight. The results were as follows: 1. The total count of leukocytes and lymphocytes decreased significantly from 14 to 21 days after treatment. 2. Mryeloid: erythroid ratios increased significantly on days 12 after treatment. 3. Delayed-type hypersensitivity skin reactions to tuberculin were reduced predominantly. 4. T-2 toxin induced prolonged prothrombin time. 5. Mitogenic responses of lymphocytes to both lipopolysaccharide and phytohemagglutinin were significantly depressed on days 7 and 14 after treatment. 6. Treatment of T-2 toxin caused marked depletion of lymphocytes in the thymus, mesenteric lymph node, peyer's patchs and spleen.

• The Korean Journal of Cytopathology
• /
• v.4 no.2
• /
• pp.146-149
• /
• 1993

Warthin's tumor is a benign salivary gland tumor, occurring primarily in the parotid gland. It's incidence is 4% of all salivary gland tumors. The cytologic finding of Warthin's tumor is characteristic and it consists of sheets of oncocytes and scattered lymphocytes in the background of granular material. We reports three cases of Warthin's tumor showing characteristic cytologic findings. The patients consisted of two males and a female and their ages were 60, 73, and 69 years, respectively. All the lesions were in the parotid gland. The cytologic findings showed sheets of oncocytic epithelial cells and scattered lymphocytes in the back-ground of granular materials and many red blood cells. In one case, the back-ground was composed of many Inflammatory cells including lymphocytes, neutrophils and macrophages.

• Tuberculosis and Respiratory Diseases
• /
• v.81 no.4
• /
• pp.351-351
• /
• 2018

• Journal of fish pathology
• /
• v.9 no.1
• /
• pp.95-109
• /
• 1996

This work was carried out to investigate the functional heterogeneity of peripheral blood lymphocytes(PBLs) in carp, Cyprinus carpio. PHA, Con A, LPS and BCG were injected intraperitoneally into carp to determine the blastogenic response and rosette formation activity. In each group of fish treated with stimulators, the cell numbers and DNA contents of lymphocytes were higher than those of untreated control group and reached the highest level between 1 week and 2 weeks after injection with mitogens. These results showed that BCG and Con A were strong stimulators of proliferation compared to PHA and LPS. However, PHA-treated fish twice showed the highest rosette formation response among the consecutive stimulations with the same mitogen. Alase, the results on consecutive mitogen stimulation revealed that carps reinjected by different mitogens led to an increased stimulation higher than the one reinjected after 1 week with same mitogen. It seems that different mitogens may stimulate different cell populations and implies functionally separated subpopulations of lymphocytes in carp.

• Korean Journal of Veterinary Research
• /
• v.29 no.4
• /
• pp.525-530
• /
• 1989

In order to enumerate the T-lymphocytes in bovine peripheral blood lymphocytes (PBL) by E rosette assay, KGRBC were treated with various concentrations of 2-aminoethylisothiouronium bromide(AET) and dextran(Dex), singly or in combination. To further standardize the assay, optimum concentration of AET- and/or Dex-treatment and incubation time for rosette forming cell(RFC) counts were determined. The levels of B-lymphocytes in the PBL were evaluated by erythzocyte-antibody($EA_{Fc}$)- and erythrocyte-antibody-complement (EAC)-rosetting techniques. The results obtained were as follows; The PBL from 20 clinically normal Korean cattle were formed as low percentage of spontaneous E-rosette ($6.7{\pm}2.4%$) in control group, whereas in KGRBC treated with 0.1M AET for 20 minutes and 8% Dex were formed as $37.3{\pm}2.7%$ and $45.1{\pm}2.1%$, respectively. And the synergistic effects were noted no less than $66.5{\pm}5.6%$ when the KGRBC treated with 0.1M AET and 8% Dex subsequently and rate of RFR did not change significantly between 3~24 hours incubation time at $4^{\circ}C$, EA-and EAC-RFR were $23.3{\pm}9.1%$ and $23.1{\pm}7.9%$, respectively. These results suggest that the KGRBC would be a useful agent for the enumeration of T-lymphocytes by E rosette assay and B-lymphocytes by EA- or EAC-rosette assay in cattle-PBL.