• Korean Journal of Veterinary Research
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• v.12 no.1
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• pp.25-35
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• 1972

The author used a dog preparation, in which either the left lung or the lower left lobe rebreathed continuously from a bag, to compare $CO_2$ and $O_2$ tensions in the rebreathed gas with those in blood entering or leaving the lung. In the 10 dogs studied, alveolar pressures for both gases were greater than in the blood; the "upstream" gradient (alveolar pulmonary arterial blood) exceeded the "downstream" gradient (alveolar-pulmonary venous blood) for both gases, but the difference was only significant for $CO_2$. The author attributed this effect to the production of $CO_2$ in lung tissue metabolism and its uptake by the blood. The difference in $CO_2$ tension between blood leaving and entering lung increased while the downstream gradient fell, an effect which was independant of the level of anesthesia. There was no evidence of a fall in base excess in blood passing through the lung, as would occur from the production of metabolic acids during anaerobic metabolism at the low levels found in the rebreathing lung in this preparation.

• Journal of Chest Surgery
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• v.13 no.2
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• pp.85-91
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• 1980

Extracorporeal circulation by hemodilution technique has been currently used with its clinical safety and good peripheral tissue perfusion in open heart surgery. There is no doubt that $O_{2}$ carrying capacity of the blood is disturbed by decreased hemoglobin level resulting from hemodilution of the circulating blood. From the view point of the blood gas exchange, these experimental studies were undertaken to determined the sate limit of hemodilution in the condition of cardiopulmonary bypass with a constant perfusion flow rate. Twelve adult mongrel dogs weighing 10 to 13 Kg. were anesthetized with pentobarbital and then respiration was controlled with Harvard volume respirator using room air. The cardiopulmonary by pass was performed by use of Sarns heart lung machine (console 5000, 5 head and 2 roller pumps) and Travenol pediatric bubble oxygenator. The perfusion rate during bypass was maintained at a constant rate of 80 ml/min/Kg of body weight. The ratio of oxygen gas flow to blood flow was kept in 3 to 1 constantly. International hemodilution was attained by serial blood withdrawals and immediate infusion of equal volumes of diluants composed of Ringer's lactate, 5% dextrose in water and 25% mannitol solution, proportionally 60%, 30%, and 10%. Arterial and venous blood samples were obtained between 15 and 20 minutes following each hemodilution. Hematocrits and hemoglobin values, $PO_{2}$, $PCO_{2}$ and pH were measured. Oxygen and carbon dioxide contents oxygen consumption and carbon dioxide elimination were calculated groups according to different hematocrit values and the correlations were evaluated. Result were as follows. 1. the arterial $O_{2}$ tension and $O_{2}$ saturation were maintained at the physiological level irrespective of the hematocrit value. 2. The venous $O_{2}$ tension and $O_{2}$ saturation showed a tendency to decline with the decrease in hematocrit value and positive correlation between them (r = +0.49, r = +0.76), The mean values of venous $O_{2}$ tension and $O_{2}$ saturation, however, were not decreased when the hematocrit levels were lower than 20%. 3. The arterial $O_{2}$ content declined lineally in proportion to the fall of hematocrit level with a positive correlation between them (r = +0.95). 4. The venous $O_{2}$ contents were decreased gradually as the hematocrit value decreased with positive correlation between them ( r =+0.89). The trend of diminution of venous $O_{2}$ content, however, was became low according to progressive decrease of hematocrit level. 5. Systemic oxygen consumption was in higher range than $O_{2}$ requirement of basal metabolism when the hematocrit value was above 20%, but abruptly decreased when the hematocrit value became to below 20%. 6. The arterial $CO_{2}$ tension and $CO_{2}$ content showed trend of increasing with progressive decrease of hematocrit value but exhibited a rather broad range and there was no relationship between those value and the hematocrit value. 7. The venous $CO_{2}$ tension and $CO_{2}$ content have also no correlation with change of Ht. value but related directly to those value of arterial blood with positive correlation between them (r = +0.78, r = +0.95_. 8. A-V difference of $CO_{2}$ content and $CO_{2}$ elimination wasnot significantly influenced by Ht. value. From the results, we obtained that feasible limit in inteneional hemodilution is above the hematocrit value of 20% under the given experimental condition.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.3 no.2
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• pp.188-193
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• 1993

Toluene in air and blood and hippuric acid in urine were checked for the 41 female workers who are exposed to toluene and have normal liver function in shoe making factories in July, 1993. Toluene in air was sampled with charcoal by personal air sampler at least 4 times and analyzed by gas chromatography. At the end of shift, blood and spot urine were collected with a vacuum tube containing EDTA and a polyethylene bottle for detecting blood toluene and urine hippuric acid and analyzed by gas chromatography and high performance liquid chromatography. Means of air and blood toluene and urine hippuric acid concentrations were 34.35ppm, 0.360mg/L, and 1.78g/g creatinine, respectively. Toluene in air showed a good correlation with hippuric acid in urine(r=0.4503) and toluene in blood(r=0.4596). The hippuric acid in urine and toluene in blood corresponded 10 exposure of 100ppm toluene in air were 2.628g/g creatinine and 0.481mg/L. Blood toluene and urine hippuric acid expected ratio were not correlated to the obesity index and working duration, however increased with age.

• Journal of Environmental Science International
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• v.23 no.8
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• pp.1429-1436
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• 2014

In this study, we investigated the effects of dietary supplementation (n = 40 pigs/treatment) with bamboo powder (0, 1, 2 and 3%) for 38 days. We evaluated growth performance, blood metabolites, and carcass characteristics of fattening pigs and gas emission and microbial populations in pig manure, to obtain data on pork producers for environmental management. We obtained the following results. First, supplementation with increasing amounts of bamboo powder had a significant (P < 0.05) effect on feed intake, feed efficiency, and glucose contents (except for initial and final body weight, weight gain, carcass characteristics, and blood urea nitrogen). In terms of blood metabolites, glucose and blood urea nitrogen tended to decrease with increasing amounts of bamboo powder. Second, the amounts of ammonia, methane, amine, hydrogen sulfide, and acetic acid were reduced by increasing amounts of bamboo powder when compared with the controls (P < 0.05). However, there were no significant differences in pH, propionic acid, iso-butyric acid, butyric acid, iso-valeric acid, and valeric acid among all treatments. The lowest gas emission was observed when 3% bamboo powder was used. Third, supplementation with increasing amounts of bamboo powder tended (P < 0.05) to increase the total number of bacteria, Lactobacillus spp., and yeast, but E. coli, Salmonella spp., and Shigella spp. were not detected in any treatment. In conclusion, the results of this study suggest that supplementation with bamboo powder was effective in reducing gas emission and inhibiting pathogen populations in pig manure by lowering the pH of the manure.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.4
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• pp.587-592
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• 2006

This study was conducted to evaluate the effects of supplementation with bacillus-based probiotic (Bacillus subtilis, $1.0{\times}10^7CFU/g$; Bacillus coagulans, $2.0{\times}10^6CFU/g$ and Lactobacillus acidophilus, $5.0{\times}10^6CFU/g$) on finishing pigs growth performance, nutrients digestibility, blood characteristics and fecal noxious gas content and to determine the optimal addition level of this probiotic preparation. A total of forty eight pigs with an initial body weight (BW) of $90.60{\pm}2.94kg$ were allotted to three dietary treatments (four pigs per pen with four pens per treatment) according to a randomized complete block design. Dietary treatment included: 1) CON (basal diet); 2) BP1 (basal diet+bacillus-based probiotic 0.1%) and 3) BP2 (basal diet+bacillus-based probiotic 0.2%). The experiment lasted 6 weeks. Through the entire experimental period, ADG was improved by 11% (p<0.05) in pigs fed diets supplemented with 0.2% bacillus-based probiotic compared to pigs fed the basal diet. ADFI and gain/feed were not affected by the treatments (p>0.05). Supplementation of bacillus-based probiotic did not affect either DM and N digestibilities or blood characteristics (p>0.05) of pigs. Fecal ammonia nitrogen ($NH_3$-N) measured at the end of experiment was reduced (p<0.05) when pigs were fed the diet with 0.2% bacillus-based probiotic. Fecal butyric acid concentration also decreased significantly (p<0.05) whereas acetic acid and propionic acid concentrations were not affected (p>0.05) when pigs were fed diets with added bacillus-based probiotic. In conclusion, dietary supplementation of bacillus-based probiotic can increase growth performance and decrease fecal noxious gas content concentration.

• Journal of Biomedical Engineering Research
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• v.24 no.2
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• pp.121-126
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• 2003

The purpose of this work was to assess and quantify whether the beneficial effects in long-term gas exchange at exciting frequency were obtained at different frequencies as well and then to develop a vibrating intravascular lung assist device(VIVLAD), for Patients suffering from acute respiratory distress syndrome(ARDS) or chronic respiratory problems. We investigate the optimal condition of the frequency band excited with new vibrator at state of limit hemolysis when blood hemolysis came to through a membrane vibration action. The experimental design and procedures were given for a device used to assess the effectiveness of membrane vibrations. Quantitative experimental measurements were performed to evaluate the performance of the device . and to identify membrane vibration dependence on blood hemolysis. We developed an analytical solution for the hydrodynamics of flow through a bundle of sinusoidally vibrated hollow fibers that is used to provide some insight into how wall vibrations might enhance the performance of the VIVLAD. In the result, it was measured that the effect of various excited frequencies in gas transfer rate and hemolysis from the maximum gas transfer rate at no vibration when the maximum gas transfer rates showed at module type 6, module type 6 consisted of 675 hollow fiber membranes The maximum oxygen transfer rate was caused by the occurrence of maximum amplitude and transfer of vibration to hollow fiber membranes when it was excited by the frequency band of 7Hz at each blood flow rate. because this frequency became the End mode resonance frequency of the flexible in blood flow. Also, when module type 6 was excited at an excited frequency of 7Hz. blood hemolysis was low. Therefore, we decided that the limit of hemolysis frequency is 7Hz . because maximum amplitude occurred at this frequency.

• Bulletin of the Korean Chemical Society
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• v.34 no.4
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• pp.1101-1107
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• 2013

In this study, total mercury and methyl mercury in whole blood of Korean was analyzed so as to investigate the correlation between total mercury (T-Hg) and methyl mercury (Me-Hg). 4000 whole blood samples were divided in four groups, according to T-Hg concentration in percentile: group I (p25-p50), group II (p50-p75), group III (p75-p95) and group IV (p95-p100). 100 samples were randomly selected from the each group, and Me-Hg concentration was measured. T-Hg concentration in whole blood was analyzed using a Direct Mercury Analyzer-80 and obtained limit of detection (LOD) was $0.2{\mu}gL^{-1}$. Me-Hg concentration was analyzed with ethylate derivatization using headspace-gas chromatography-mass spectrometry, and obtained LOD of methyl mercury was $0.5{\mu}gL^{-1}$. The geometric means of T-Hg and Me-Hg were $6.35{\mu}gL^{-1}$ and $4.44{\mu}gL^{-1}$, respectively, and 71.91% of T-Hg was presented as Me-Hg.

• Tuberculosis and Respiratory Diseases
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• v.48 no.5
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• pp.773-780
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• 2000

Background : pH measurement is an important test in assessing the etiology of pleurisy and in identifying complicated parapneumonic effusion. Although the blood gas analyzer is the gold standard method' for pleural pH measurement, pH meter & pH strip methods are also used for this purpose interchangably. However, the correlation among the pH data measured by the three different methods needs to be evaluated. In this study, we measured the pH of pleural fluid with the three different methods respectively and evaluated the correlation among the measured data. Methods : From August 1999 to March 2000, we measured the pleural fluid pH in 34 clinical samples with three methods-blood gas analyzer, pH meter, and pH strip. In the blood gas analyzer and pH meter methods, the temperature of pleural fluid was maintained around $0^{\circ}C$ in air-tight condition before analysis and measurement was performed within 30 minutes after collection. As for the pH strip method, the pleural fluid pH was checked in the ward immediately after tapping and in the clinical laboratory of our hospital. This part is unclear. Results : The causes of pleural effusion were tuberculosis pleurisy in 16 cases, malignant pleural effusion 5 cases, parapneumonic effusion 9 cases, empyema 3 cases, and congestive heart failure 1 case. The pH of pleural fluid (mean$\pm$SD) was 7.34$\pm$0.12 with blood gas analyser, 7.52$\pm$0.25 with pH meter, 7.37$\pm$0.16 with pH strip of immediate measurement and 6.93$\pm$0.201 with pH strip of delayed measurement. The pH measured by delayed pH strip measurement was lower than those of other methods (p<0.05). The correlation of the results between the blood gas analyzer and pH meter(p=0.002, r=0.518) and the blood gas analyzer and pH strip of immediate measurement(p<0.001, r=0.607). Conclusion : In the determination of pH of pleural fluid, pH strip method could be a simple and reliable method under immediate measurement conditions after pleural fluid tapping.

• Journal of Veterinary Clinics
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• v.32 no.6
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• pp.499-503
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• 2015

The objective of this study was to investigate arterial and venous blood gas, electrolytes, biochemical, and hematological values in healthy Korean native calves (KNC). The healthy 62 KNC within 3 weeks-old were examined. The arterial blood was collected from caudal auricular artery and the venous blood from jugular vein. The blood samples were analyzed immediately using a portable blood gas analyzer. The pH, $pO_2$, $pCO_2$, $cHCO_3{^-}$, BE, $cSO_2$, $Na^+$, $Ca^{2+}$, $Cl^-$, anion gap potassium (AgapK), Hct, cHgb, glucose, lactate and creatinine were determined. The normal values for blood gas, electrolytes, biochemical, and hematological variables determined in this study agree with other published values for normal calves. The mean concentration of glucose and lactate within 3 weeks old of KNC is higher than those of adult cattle. The blood values according to weeks of age within 3 weeks-old of arterial and venous blood variables were not significantly different (P > 0.05). Glucose (r = 0.927) had the strongest correlations between arterial and venous values. The correlation between the values of the arterial and the venous blood was strong in creatinine (r = 0.925), lactate (r = 0.815), $Ca^{2+}$ (r = 0.806), Hct (r = 0.799), $Na^+$ (r = 0.790), cHgb (r = 0.786), base excess (r = 0.749), pH (r = 0.710), $HCO_3{^-}$ (r = 0.710), and $cTCO_2$ (0.663). Analysis of blood samples in a field condition, using hand-held analyzer is rapid and useful in bovine practice.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.2
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• pp.218-225
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• 2017

To investigate the anti-hangover effects of Cudrania tricuspidata root extract (CTE), the blood alcohol metabolism and blood gas imbalance of CTE in rats treated with 10 ml/kg alcohol were examined. CTE (500 mg/kg and 750 mg/kg) was administrated after 30 minutes of alcohol consumption (10 ml/kg). Blood collection was implemented from the tails of the animals after 1, 3, and 5 hours post alcohol consumption. The Condition drink (a commercial anti-hangover beverage) was used as a positive control. Single administration by the oral route was performed. The consumption of CTE (500 mg/kg and 750 mg/kg) decreased the serum alcohol concentration by increasing and maintaining both the alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) enzyme activity levels in the blood and liver. In addition, CTE led to recovery from the imbalances in the blood gas levels, including carbon dioxide ($CO_2$) and changes in pH, bicarbonate ($HCO_3{^-}$) and lactic acid levels due to alcohol ingestion. In conclusion, CTE exerted a more pronounced anti-hangover effect than a commercial anti-hangover drink. Therefore, CTE can be a novel and safe anti-hangover natural product agent for the prevention or treatment of symptoms caused by excessive alcohol consumption.