• Title/Summary/Keyword: Blood collection

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Changes in the Saliva Cortisol Concentration and Blood Pressure of Patients with High Blood Pressure and Diabetes After Participating in Agro-healing Activities

  • Jang, Hye Sook;Yoo, Eunha;Jeong, Sun-Jin;Kim, Jae Soon;Gim, Gyung Mee;Ryu, Doo Young
    • Journal of People, Plants, and Environment
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    • v.22 no.5
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    • pp.445-454
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    • 2019
  • This study was conducted to find out physical changes in the blood pressure and saliva cortisol of patients with high blood pressure and diabetes after participating in agro-healing activities. The saliva cortisol and blood pressure of 14 males and females aged 49.79±10.48 on average were measured after participating in agro-healing activities. The saliva collection system was used, and their satisfaction level for the program was also surveyed. Changes in the values before and after participating in the agro-healing activities were compared and analyzed. The cortisol concentration, a stress hormone, was reduced from 6.76nmol/L before the program to 4.86nmol/L after the program, down by 1.9nmol/L, which was a statistically significant difference (z=-3.170, p<.01). In addition, the cortisol concentration was gradually reduced through the entire program from Session 1 to Session 7. Changes in the blood pressure of subjects before and after participating in agro-healing activities were examined, and the total systolic and diastolic blood pressure continuously decreased after participating in agro-healing activities through the entire program from Session 1 to Session 7. Their systolic blood pressure was reduced statistically significantly (z=-1.947, p<.05). Also, participants' satisfaction was analyzed and it was found that more than 95% of participants answered 'satisfied' and 'very satisfied' with agro-healing activities. Therefore, these results indicate that agro-healing activities performed in the healing-farm had a positive impact on the cortisol concentration and blood pressure of patients with chronic diseases such as diabetes and high blood pressure. In addition, their high satisfaction for this program seemed to improve the psychological and physiological conditions of subjects.

A Study on Nutritional Status of Iron in the Rural Elderly (일부 지역 농촌 노인의 철분 영양상태에 관한 연구)

  • 신소영;최미경;이윤식;승정자
    • Journal of the East Asian Society of Dietary Life
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    • v.6 no.2
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    • pp.135-142
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    • 1996
  • The purpose of this study was to examine nutritional status of iron in 27 rural elderly subjects. The daily intake, urinary excretion and serum level of iron were determined by 24-hr food collection method, and 24-hr urine and serum collection for 3 days. The results were summarized as follows. 1. The mean age, height, weight, and blood pressure were 67.7 years, 163.2cm, 55.9kg, and 121.1/80. 0mmHg in men and 69.9 years, 146.5cm, 47.6kg, and 108.9/71.1mmHg in women, respectively 2. The daily intake of energy, protein, vitamin A, vitamin B$_1$, vitamin B$_2$, niacin, vitamin C, and iron were lower than Korean recommended dietary allowances. 3. The daily iron intake was 7.82mg. The proportion of heme iron and nonheme iron to the total iron intake were 8.05% and 91.94%, respectively Major iron sources of the rural elderly were cooked rice, Ko Chu Jang, beef blood, anchovy, soybean paste, and leafy radish. 4. The hemoglobin, hematocrit, serum iron, and total iron binding capacity were 14.3g/㎗, 42.9%, 79.4 $\mu\textrm{g}$/㎗, and 268.4 $\mu\textrm{g}$/㎗ in men and 12.9g/㎗, 38.8%,92.4 $\mu\textrm{g}$/㎗, and 277.9$\mu\textrm{g}$/㎗ in women, respectively. 5. There was a significant positive correlation between dietary intake and urinary excretion of iron.

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Prevalence of Feline Blood Types in Seoul and Kangwon Area of Korea (서울 및 강원지역 고양이의 혈액형 빈도 조사)

  • Ban, Ji-Min;Shin, Ji-Hye;Kim, Jae-Young;Hyun, Chang-Baig;Kim, Doo;Pak, Son-Il
    • Journal of Veterinary Clinics
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    • v.25 no.4
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    • pp.227-230
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    • 2008
  • To determine the distribution of feline blood types and then to estimate the risk of neonatal isoerythrolysis (NI) in non-pedigree cats, we typed blood of 482 cats of both genders and various breeds (336 domestic shorthair cat and 146 pedigree) from August 2005 through July 2007. Blood samples from Seoul and Kangwon province were typed within 5 days after collection by the simple tube method. High-titer anti-A antiserum and anti-B reagent, prepared with Triticum vulgaris lectin, were used to determine type A and type B blood, respectively. The majority of cats were type A (n = 465, 96.5%) and only 3.5% (n = 17) were type B. No type AB blood were detected. Blood type distributions among the non-pedigree and pedigree cats were similar: for non-pedigree cats, 96.4% were type A and 3.6% were type B, whereas for pedigree cats, 96.6% were type A and 3.4% were type B. All type B cats had a very strong agglutination reaction to anti-A antiserum: 8 sample for 3+ and 9 for 4+. Assuming 19% of estimated frequency for the type-B allele in domestic cats, the calculated proportion of random mating from this population at risk for developing NI was 3.4%. Based on this finding, it is strongly recommended that blood typing be performed prior to any blood transfusion or breeding to minimize blood type incompatibilities. Further comprehensive studies on the titer of naturally occurring antibodies in cat populations in Korea and the prevalence of possible NI in practice are clearly required.

Preanalytical Stability of Adrenocorticotropic Hormone Depends on Time to Centrifugation (전혈에서 시간과 온도에 따른 Adrenocorticotropic Hormone의 안정성 평가)

  • Shin, Sun-Young;Lee, Hyun-Joo;Min, Gyung-Sun;Lee, Ho-Young
    • The Korean Journal of Nuclear Medicine Technology
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    • v.13 no.1
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    • pp.116-119
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    • 2009
  • Background: Preanalytical factors can affect reliability of hormone assay results. Adrenocorticotropic hormone (ACTH) in blood is considered highly unstable because of proteolytic degradation, so storage of blood samples on ice until analysis is recommended. In clinical practice, however, this procedure may present logistical problems because most samples for ACTH measurement must be shipped from the place of sample collection to the laboratory. Therefore, we studied the impact of time and temperature before plasma separation and analysis on the results of ACTH assays. Methods: A total number of 22 patients were enrolled in this study. We obtained 2 blood samples. ACTH concentrations were 35~126 pg/mL. ACTH concentrations were measured by immunoradiometric assay (IRMA) using commercial kits (CIS Biointernational, Gif-sur-Yvette, France). Results: ACTH levels showed a significant difference between the samples of $22^{\circ}C$ EDTA and $4^{\circ}C$ EDTA. Measured ACTH concentrations significantly decreased with time before freezing at $-20^{\circ}C$. ACTH levels showed no significant difference between the groups of after storage for 24 hr without centrifugation at $22^{\circ}C$ and $4^{\circ}C$. Conclusion: We recommend that blood samples be obtained on pre-chilled EDTA collection tubes. The shortest possible time between sample collection and processing is always the best laboratory practice.

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Blood Mercury Concentration and Related Factors in an Urban Coastal Area in Korea

  • Jo, Eun-Mi;Kim, Byoung-Gwon;Kim, Yu-Mi;Yu, Seung-Do;You, Chang-Hun;Kim, Joon-Youn;Hong, Young-Seoub
    • Journal of Preventive Medicine and Public Health
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    • v.43 no.5
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    • pp.377-386
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    • 2010
  • Objectives: This study was carried out for the purpose of evaluating the blood mercury concentration of the residents of Busan, Korea, as well as the relationship between the mercury concentration and the pattern of fish consumption along with other epidemiological factors. Methods: Two hundred ninety-three subjects (147 men and 146 women), who were aged 40 years or more, were recruited into this study between June and October 2009. The mean age of the subjects was 54.3 years (with a range of 40-70 years). Mercury concentrations in blood samples were measured using a gold-amalgam collection method. Results: The geometric mean concentration of mercury in the total subjects was $8.63\;{\mu}g/L$ [range: $1.48{\sim}45.71\;{\mu}g/L$]. The blood mercury concentration of the men ($9.55\;{\mu}g/L$) was significantly higher than that of the women ($7.76\;{\mu}g/L$). The blood mercury concentration of those who eat fish more than 4 times per week was higher than others, and was statistically significant (male p = 0.0019, female p = 0.0002). According to the multiple analysis, the blood mercury concentration was significantly affected by the consumed fish but other epidemiological factors were not related. Conclusions: It was found that the subjects who have consumed a large amount of fish may have high blood mercury concentration. It appears that fish consumption can influence blood mercury concentration. Therefore, guidelines for fish consumption that will decrease blood mercury concentration might be necessary in Korea.

Clinical features of COVID-19 as presented in journal articles : A Korean Medical Approach (COVID-19 임상표현에 대한 한의학적 접근 -국내외 논문을 중심으로-)

  • Kim, Jong-hyun;Ahn, Jinhee;Kim, Sanghyun
    • Journal of Korean Medical classics
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    • v.35 no.1
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    • pp.1-32
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    • 2022
  • Objectives : This paper examines major symptoms representation in COVID-19 patients as groundwork for development of an effective clinical data collection format in Korean Medicine. Methods : Major symptoms representation in COVID-19 related papers published worldwide were collected. Corresponding symptoms in Korean Medicine were then examined, followed by discussion of symptomatic features that require further consideration in regards to a more systematic clinical data collection. Results : Of 256 papers, most papers listed fever and cough while symptoms such as difficulty breathing, diarrhea, muscle pain, headache, nausea, fatigue, chest pain, phlegm, nasal discharge were also mostly listed. Clinical representations could be categorized into general symptoms, throat symptoms, chest symptoms, head and facial symptoms, gastrointestinal symptoms, musculo-skeletal and cutaneous symptoms, psychiatric symptoms and sensory problems. Conclusions : Although each clinical representation could be likened to certain clinical representations of Korean Medicine, the variety of symptoms were too limiting and lacking in detail to be applied in the pattern identification[辨證] of Korean Medicine. For effective clinical data collection and analysis in the future, symptom change according to time, comparison between location, climate and ethnicity, existence of interior symptoms when diagnosing exterior symptoms, deficiency-excessiveness of blood patterns, consciousness levels, etc., need to be considered in establishing criteria for symptom evaluation.

Factors Affecting University Students' Motivation to Participate in Blood Donation (대학생의 헌혈참여 동기에 영향을 미치는 요인)

  • Jo, Nam-Hee;Lee, Soon-Young
    • Journal of Convergence for Information Technology
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    • v.10 no.11
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    • pp.232-239
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    • 2020
  • The purpose of this study was to identify the factors affecting the motivation for blood donation participation of university students. The subjects of this study were 200 university students in one area, and a self-report questionnaire was used for data collection. The data analysis used frequency, percentage, mean, standard deviation, Independent T-test, One-way ANOVA, Pearson Correlation Coefficient, and Multiple Regression Analysis. As a result of the study, the factors influencing the subjects' motivation to participate in blood donation were significant in the order of attitude toward blood donation, future blood donation plan, perceived health status, altruism, and number of blood donation experiences. The results of this study can be used as basic data for the development of educational programs to increase the motivation for blood donation participation of university students. It also suggests that there is a need to develop strategies that can increase university students' attitudes toward blood donation, altruism, and health status.

A Case of Epidural Blood Patch to Treat Headache Due to Spontaneous Intracranial Hypotension -A case report- (경막외 혈액봉합술을 이용한 자연성 두개내 저압성 두통 환자의 치험 1예 -증례 보고-)

  • Park, Soo-Seog;Jang, Yeon;Cho, Eun-Chung;Jee, Seung-Eun;Song, Ho-Kyung;Jung, Sung-Woo
    • The Korean Journal of Pain
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    • v.11 no.2
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    • pp.338-342
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    • 1998
  • Postural headache due to spontaneous intracranial hypotension occurs without any diagnostic lumbar puncture, myelography, cranial or spinal injury, or spinal anesthesia. The clinical characteristics of the syndrome are disappearance of the headache or a notable decrease in its severity with recumbency, the finding of meningeal enhancement and subdural fluid collection on brain MRI, the pleocytosis and the increased CSF protein concentration. We report a case of a 40-year-old woman who exhibited the signs and symptoms of postural headache due to low CSF pressure. Her headache started suddenly at the occiput and radiated to frontal head. Magnetic resonance imaging (MRI) of her brain showed enhancement of the meninges and subdural fluid collection. Intrathecal radionuclide cisternography showed the delayed appearance of the isotope in the cranium and the minimal CSF leak at the left upper thoracic region. Her headache was relieved completely after a lumbar (L2-L3) epidural injection of 12 ml of autologous blood and remained asymptomatic.

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Milk Yield and Immune Response of Periparturient and Early Lactation Friesian Cows Fed Diets Supplemented with a High Level of Amino-acid Chelated Chromium

  • Terramoccia, S.;Bartocci, S.;Lillini, E.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.8
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    • pp.1098-1104
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    • 2005
  • The trial was carried out on twenty-one Friesian cows at the end of eight months gestation, nine multiparous and twelve primiparous; allocated into three groups (1 control, 2 and 3 experimental). The same diet was administred to all three groups before partum (12.8 kg DM/head/day) and after partum (18.8 kg DM/head/day). The cows in groups 2 and 3 received two different daily quantities of amino-acid chelated chromium (0.6 and 1.2 mg Cr/kg DM) from 4 weeks prior to presumed parturition to 6 weeks after. The milk yield control was carried out at 15, 30, 42 and 60 days. All animals were immunised two weeks prior to the presumed parturition and two weeks after with the following antigens: ovalbumin and brucellergene. Blood samples were collected weekly to monitor humoral and cell-mediated immune responses. When analysing the results of antibody immunity (ovalbumin) in the sixth blood collection both treated groups significantly increased compared to group 1 (0.5230 and 0.4536 vs. 0.1812 OD; p<0.05). The results of the cell-mediated immune response (brucellergene) had significant differences (p<0.10) in correspondence to the third (between group 2 and control) and the fifth (between groups 3 and 2) blood collection. Significant differences in fat corrected milk were observed at 42 days between group 3 and the other two groups (31.01 vs. 26.99 and 28.66 kg/d, p<0.05) and at 60 days between group 3 and control (30.88 vs. 26.69 kg/d, p<0.05). Before partum and at partum a positive immune response was obtained with a lower dose of chromium. After partum a positive immune response, anti-OVA indicator, was obtained with the higher dose of chromium while, $\gamma$-IFN indicator, with the lower dose. A significant increase of the milk yield resulted at both 42 and 60 days with the highest level of chromium.

Relationship among Dietary Intakes, Blood Levels, and Urinary Excretions of Ca, P, Mg and Serum Lipid Levels in Korean Rural Adult Men and Women (일부 농촌 성인 남녀의 칼슘, 인, 마그네슘의 섭취량, 혈액수준 및 소변중 배설량과 혈청지질수준과의 관계)

  • 승정자;노숙령;김애정;최미경;이주희
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.6
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    • pp.709-715
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    • 1993
  • This study was carried out to estimate the relationship among dietary intakes, blood levels, and urinary excretions of Ca, P, Mg and serum lipid levels in 30 healthy adults living area of Korea(12males and 18 females). Analyses for the nutritional status of the subjects were performed by 3-day dietary intake record, duplicated diet collection, 24-hours urine collection, and venous blood sampling before anthropometry. The mean daily intakes of Ca, P and Mg estimated for 3 days were 452.5$\pm$159.5mg, 725.6$\pm$ 567.8mg and 240.4$\pm$78.5mg and the serum concentrations of Ca, P and Mg were 8.9$\pm$ 0.5mg / dl, 3.3$\pm$0.6mg / dl, and 2.1$\pm$0.8mg /dl, respectively. The 24-hour urinary excretions of Ca, P and Mg were 80.4$\pm$24.0mg, 399.0$\pm$161.1mg and 64.8$\pm$35.3mg, respectively. The serum concentrations of total cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol were 158.8$\pm$32.9mg/dl, 95.6$\pm$43.9mg/dl, 45.7$\pm$9.9mg/dl and 92.2$\pm$ 28.5mg/dl, respectively. In the relation between Ca, P, Mg and serum lipids it showed positive correlation with serum Ca and HDL-cholesterol at the level of significance of 5%. It can be concluded that the high level of Ca in serum may be useful in the prevention of cardiovascular disease, but the study verifies the need for more systematic studies.

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