• 대한한방부인과학회지
• /
• 제19권2호
• /
• pp.1-17
• /
• 2006

Purpose : The Purpose of this research was to investigate the effects of antithrombotic activities of Haenggyonghonghwatang (HGHHT). Methods : Measured the effect which was given to blood flow rate through the regular volume of glass tube after the blood was diluted five times with ACD soulution. Antithrombotic effect was calculated as a percentage of the experimental animal figure protected from the paralysis of hind legs or death of the mouse that is caused from the administration of platelet aggregation regent. Each of the groups consisted in 8 mice, was divided into Normal, Control, and HGHHT. All of these 3 group were supplied a saline solution and after an hour the control group brought the dextran extravasated blood. Also the HGHHT group was dosed to the experimental mice with Oral Zonde one day before the experiment. After that, the mice were abstained from food. And then we gave a measured amount of it before an hour. Finally, it gave rise to dextran extravasated blood as well as the Control group. Results : The results were obtained as follows. HGHHT inhibited platelet aggregation induced by ADP and epinephrine significantly as compared with the control group. HGHHT showed fibrinolytic activity insignificantly as compared with the control group. HGHHT increased blood flow rate significantly as compared with the control group in vitro, but insignificantly as compared with the control group in vivo. HGHHT inhibited pulmonary embolism induced by collagen and epinephrine(inhibitive rate 50%). HGHHT increased number of platelet, fibrinogen amount and shortened prothrombin time, activated partial thromboplastin time significantly as compared with the control group in thrombus model induced by dextran. Conclusion : HGHHT is effective antithrombotic activity from experimental result.

• 폴리머
• /
• 제25권3호
• /
• pp.343-348
• /
• 2001

Perfluorinated polyether diol(Fomblin ZDOL$ZDOL^{(R)}$)과 4,4'-diphenyl methane diisocyanate(MDI)로부터 합성된 불소화된 폴리우레탄 탄성체의 기계적 성질과 혈소판 점착 특성을 연구하였다. Fomblin 함량과 혼합 사용된 polyether polyol의 종류에 따른 기계적 성질의 검토를 인장실험 및 dynamic mechanical analysis를 통해 행하였다. 또한 혈소판 점착실험을 통해 불소화된 폴리우레탄 탄성체의 혈액적합성을 평가하였다. 기계적 물성은 혼합된 polyether polyol의 종류와 함량에 영향을 받았고, 혈소판 점착은 모든 불소화된 폴리우레탄에서 불소화 함량의 증가에 따라 감소하였다.

• Archives of Pharmacal Research
• /
• 제26권9호
• /
• pp.723-726
• /
• 2003

Five coumarins, isoimperatorin (1), pabulenol (2), isooxypeucedanin (3), oxypeucedanin hydrate (4) and osthol (5) were isolated from the MeOH extract of Angelica genuflexa in the course of searching for anti-platelet and anti-coagulant components from plants. Pabulenol (2) was isolated from A. genuflexa for the first time. The five compounds isolated from A. genuflexa, together with decursinol angelate (6), decursin (7) and nodakenin (8) from A. gigas were evaluated for their effects on platelet aggregation and blood coagulation. Compounds 2, 5, 6 and 7 were observed to be either equally effective or 2∼4 times more inhibitory than ASA in both arachidonic acid and U46619 ($TXA_2$ mimetic) induced platelet aggregations.

• 약학회지
• /
• 제58권5호
• /
• pp.322-327
• /
• 2014

In this study, we investigated whether the mixtures of onion water extract and aloe ethanol extracts have antiplatelet activities. The mixtures inhibited collagen- and thrombin-induced rat platelet aggregation in vitro. Additionally, the oral administration of the mixtures inhibited platelet aggregation induced by collagen ex vivo but not prolonged mouse tail vein bleeding time in vivo. These results suggest that the combination of onion and aloe extracts has a potential to be a preventive agent against platelet-mediated disorders.

• Journal of Chest Surgery
• /
• 제31권10호
• /
• pp.952-963
• /
• 1998

연구배경 : 체외 순환으로 인한 지혈 기전의 심각한 손상은 술후 과다 출혈을 일으켜 환자의 생명을 위협하고, 대량 수혈에 따른 부작용과 재수술의 우려마저 증가시킬 수 있다. 특히, 심장 수술동안 섬유소 용해계의 활성화와 혈소판의 숫적, 기능적 변화가 술후 상당 시간동안 출혈의 원인이 된다고 보고하고 있다 대상 및 방법 : 본 연구는 체외 순환 동안 및 그 후에 있어, 체외 순환에 의한 섬유소 용해계의 활성화를 포함한 다양한 혈액학적 변화를 조사하고, 이러한 변화들이 술후 비외과적 출혈의 정도와 어떤 상관성이 있는가를 규명하기위해 체외 순환을 실시할 성인 환자 20명을 대상으로 전향적으로 계획되었다. 혈소판 수, 섬유소원 및 plasminogen 농도, FDP, D-dimer, BT, aPTT, PT와 같은 혈액학적 변수의 측정을 위해 수술동안 또는 술후 시기에 혈액을 연속적으로 채취하였다. 출혈량은 흉부 삽관을 통한 배출량을 술후 3, 6, 12, 24, 48시간의 양과 총혈액량을 각각 측정하였다. 결과 : 연구 결과를 요약하면 다음과 같다:혈소판 수는 체외 순환으로 인해 급격히 감소되었다 (p<0.01). 총 체외 순환 시간이 길수록 혈소판 수의 감소률은 더 높았으며 (r=0.55, p=0.01) 술후 7일이 되어서야 비로소 술전치에 근접하였다. 체외 순환동안 FDP와 D-dimer의 농도가 의미있게 증가하였고 (p<0.0001), 섬유소원 및 plasminogen 농도 둘 다 서로간에 상관성을 보이면서 감소하였다 (r=0.57, p<0.01). 술후 2hr-BT, 2hr-PT, 2hr-aPTT 모두 술전치에 비해 유의하게 연장되었다(각각 p<0.05, p<0.0001, p<0.0001). 술후 총 출혈량과 환자의 연령, 대동맥 차단 시간, 체외 순환 시간은 각각 양의 상관관계 (positive correlation)가 있었다. 술전 기준치 혈소판 수와 술후 3시간 출혈량, 체외 순환 종료시의 혈소판 수와 술후 3시간, 6시간 및 총 출혈량 그리고 술후 1일의 혈소판 수와 48시간 출혈량 사이에는 각각 음의 상관관계 (negative correlation)가 있었다. 술후 2hr-aPTT는 술후 6시간 및 48시간대의 출혈량에 상관성이 있었고 (r=0.53, p=0.02) 술후 2hr-PT는 48시간 출혈량과 각각 양의 상관성이 있었으나 (r=0.43, p=0.05). 이 두가지 변수와 총 출혈량 간에는 상관성이 없었고, 술후 2hr-BT와 술후 출혈량 사이에도 유의한 상관성을 발견하지 못하였다. 결론 : 이상의 관찰 결과들은 체외 순환이 섬유소 용해계의 활성화와 혈소판 수의 심한 감소를 포함한 다양한 혈액학적 변화를 유도하고 환자의 연령, 혈소판 수, 대동맥 차단 시간, 체외 순환 시간, 술후 PT 및 aPTT와 같은 다인적 상황들이 술후 출혈에 영향을 미친다는 점들을 시사하고 있다. 본 연구 결과들은 체외 순환에 따른 지혈적 손상에 관한 이해와 술후 출혈을 감소시키기 위한 기초 자료로 활용 가능하리라 사료된다.

• Journal of Ginseng Research
• /
• 제47권5호
• /
• pp.638-644
• /
• 2023

Background: The anti-platelet activity of the saponin fraction of Korean Red Ginseng has been widely studied. The saponin fraction consists of the panaxadiol fraction (PDF) and panaxatriol fraction (PTF); however, their anti-platelet activity is yet to be compared. Our study aimed to investigate the potency of anti-platelet activity of PDF and PTF and to elucidate how well they retain their anti-platelet activity via different administration routes. Methods: For ex vivo studies, Sprague-Dawley rats were orally administered 250 mg/kg PDF and PTF for 7 consecutive days before blood collection via cardiac puncture. Platelet aggregation was conducted after isolation of the washed platelets. For in vitro studies, washed platelets were obtained from Sprague-Dawley rats. Collagen and adenosine diphosphate (ADP) were used to induce platelet aggregation. Collagen was used as an agonist for assaying adenosine triphosphate release, thromboxane B2, serotonin, cyclic adenosine monophosphate, and cyclic guanosine monophosphate (cGMP) release. Results: When treated ex vivo, PDF not only inhibited ADP and collagen-induced platelet aggregation, but also upregulated cGMP levels and reduced platelet adhesion to fibronectin. Furthermore, it also inhibited Akt phosphorylation induced by collagen treatment. Panaxadiol fraction did not exert any antiplatelet activity in vitro, whereas PTF exhibited potent anti-platelet activity, inhibiting ADP, collagen, and thrombin-induced platelet aggregation, but significantly elevated levels of cGMP. Conclusion: Our study showed that in vitro and ex vivo PDF and PTF treatments exhibited different potency levels, indicating possible metabolic conversions of ginsenosides, which altered the content of ginsenosides capable of preventing platelet aggregation.

• 약학회지
• /
• 제44권6호
• /
• pp.558-565
• /
• 2000

SK-1080 is one of the newly developed orally active nonpeptide angiotensinII $AT_1-receptor$ antagonist that selectively acts at $AT_1$ receptor with high affinity. The cardiac effect on ischemia/reperfusion injury of SK-1080 was compared with those of losartan, a prototype of this class, in isolated rat hearts. Isolated perfused rat heart was pretreated with drug for 10 min and then subjected to global ischemia for 30 min followed by reperfusion with- or without drug for 30 min. The possible additive effect of SK-1080 on the platelet aggregation and coagulation in human blood was also studied. We investigated whether SK-1080 effects the platelet aggregation induced by ADP, a platelet agonist partially dependent on $thromboxaneA_2$. The clotting times in the prothrombin time (PT) and activated partial thromboplastin time (APTT) were also examined in human plasma in vitro as coagulation screening test. SK-1080 improved reperfusion function (LVDP, left ventricular developed pressure; PRP, rate-pressure product) in a dose-dependent manner. SK-1080 reduced ADP-induced platelet aggregation compared with vehicle but less than losartan, and did not affect clotting times.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권14호
• /
• pp.5933-5937
• /
• 2015

Background: We aimed to investigate the prognostic value of baseline neutrophil, lymphocyte, and platelet counts along with the neutrophil-lymphocyte ratio (NLR) and platelet-lymphocyte ratio (PLR) in local and advanced gastric cancer patients. Materials and Methods: In this retrospective cross-sectional study, a total of 103 patients with gastric cancer were included. For all, patient characteristics and overall survival (OS) times were evaluated. Data from a complete blood count test including neutrophil, lymphocyte, monocyte, white blood cell (WBC) and platelet (Plt) count, hemoglobin level (Hb) were recorded, and the NLR and PLR were obtained for every patient prior to pathological diagnosis before any treatment was applied. Results: Of the patients, 53 had local disease, underwent surgery and were administered adjuvant chemoradiotherapy where indicated. The remaining 50 had advanced disease and only received chemotherapy. OS time was $71.6{\pm}6$ months in local gastric cancer patients group and $15{\pm}2$ months in the advanced gastric cancer group. Univariate analysis demonstrated that only high platelet count (p=0.013) was associated with better OS in the local gastric cancer patients. In contrast, both low NLR (p=0.029) and low PLR (p=0.012) were associated with better OS in advanced gastric cancer patients. Conclusions: This study demonstrated that NLR and PLR had no effect on prognosis in patients with local gastric cancer who underwent surgery and received adjuvant chemoradiotherapy. In advanced gastric cancer patients, both NLR and PLR had significant effects on prognosis, so they may find application as easily measured prognostic factors for such patients.

• Archives of Plastic Surgery
• /
• 제38권4호
• /
• pp.345-350
• /
• 2011

Purpose: Although platelet-rich plasma (PRP) potentiate the wound healing activity of adipose-derived stem cells (ADSCs), its effect cannot be sustained for a prolonged period of time due to short duration of action. This led us to design and produce platelet-rich fibrin (PRF), in an effort to develop a tool which lasts longer, and apply it on wound healing. Methods: Two symmetrical skin defects were made on the back of seven nude mice. ADSCs were applied to each wound, combined with either PRP or PRF. The wound area was measured over 14 days. By day 16, the wound was harvested and histologic analysis was performed including counting of the blood vessel. Results: The healing rate was more accelerated in PRP group in the first 5 days (p<0.05). However, PRF group surpassed PRP group after 6 days (p<0.05). The average number of blood vessels observed in the PRF group was $6.53{\pm}0.51$, compared with $5.68{\pm}0.71$ for the PRP group. Conclusion: PRF exerts a slow yet pervasive influence over the two-week course of the wound healing process. Thus, PRF is probably more beneficial for promoting the activity of ADSCs for a sustained period of time.

• 대한의생명과학회지
• /
• 제18권2호
• /
• pp.131-138
• /
• 2012

Autologous peripheral blood stem cell transplantation (PBSCT) has been used as a major treatment strategy for hematological malignancies. The number of CD34 positive cells in the harvested product is a very important factor for achieving successful transplantation. We studied the factors that can predict the number of CD34 positive cells in the harvested product of acute myelocytic leukemia (AML), multiple myeloma (MM) and Non-Hodgkin's lymphoma (NHL) patients after mobilizing them with chemotherapy plus G-CSF. A total of 73 patients (AML 19 patients, MM 28 patients, NHL 26 patients) with hematological malignancies had been mobilized with chemotherapy and granulocyte colony-stimulating growth factor from April, 2000 to February, 2012. Group's characteristics, checkup opinion of pre-peripheral blood on the day of harvest & outcome of PBSC were analyzed and evaluated using SPSS statistics program after grouping patients as below; group 1: CD34 cell counts < $2{\times}10^6/kg$ (n=16); group 2: $2{\times}10^6/kg{\leq}CD34$ cell counts < $6{\times}10^6/kg$ (n=32); group 3: CD34 cell counts ${\geq}6{\times}10^6/kg$ (n=25). We analyzed the clinical characteristics, the peripheral blood (PB) parameters and the number of CD34 positive cells in the PB and their correlation with the yield of CD34 positive cells collected from the mobilized patients. The total number of leukapheresis sessions was 263 (mean: 3.55 session per patient), and the mean number of harvested CD34 positive cells per patient was $7.37{\times}10^6/kg$. The number of CD34 positive cells in product was significantly correlated with the number of platelet and CD34 positive cells in peripheral blood (P<0.05). The number of PB CD34 positive cells was the best significant factor for the quantity of harvested CD34 positive cells on the linear regression analysis (P<0.05). Many factors could influence the mobilization of peripheral blood stem cells. Platelet count and PB CD34 positive cells count were the two variables which remained to be significant in multivariate analysis. Therefore, the number of platelet and CD34 positive cells in peripheral blood on the day of harvest can be used as an accurate predictor for successful peripheral blood stem cell collection.