• 대한임상검사과학회지
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• 제43권3호
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• pp.113-119
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• 2011

This study was designed to determine the effects of larval extracts from Protaetia brevitarsis seulensis shade dried larva from Korea and China on liver tissues of hepatic injury groups. The experimental groups are divided into five groups; Normal, carbon tetrachloride single injection ($CCl_4$), Pb-CHI ($CCl_4$ + P. brevitarsis shade dried larva from China), Pb-KOR ($CCl_4$ + P. brevitarsis shade dried larva from Korea) and SIL ($CCl_4$ + 0.35% silymarin) groups. Sprague Dawley rats were oral injected with $CCl_4$ at a dose l mg/kg (20% in corn oil) for induction of liver damage for 4 weeks. Each experimental group was fed with a dose 50 mg/kg of larval extracts based on medicinal preparations from 3 weeks to 4 weeks after $CCl_4$ treatment. At the end of 4 weeks, we evaluated the serum levels of glutamate-oxaloacetate transaminase (GOT), glutamate-pyruvate transferase (GPT), alkaline phosphatase (ALP) and blood urea nitrogen (BUN) in serum and the cytokine levels of tumor necrosis factor-${\alpha}$) (TNF-${\alpha}$) and transforming growth factor-${\beta}$ (TGF-${\beta}$) in the cells isolated from spleen and liver. The histological analysis was also conducted. The $CCl_4$ injection reduced body weight, induced congestion of middle lobe and hepatocytic degeneration, resulting in disintegration of hepatic cords, and increased biochemical markers of blood related to hepatic injury. On the other hand, the Pb-CHI and Pb-KOR group decreased the levels of biochemical markers in blood and cytokine levels in spleen and liver. Especially, the Pb-KOR group facilitated the recovery of biochemical values of blood related to hepatic injury, hepatic lesions and fibrosis. Taken together, larval extract from P. brevitarsis might prevent acute hepatotoxicity and enhance the recovery of liver fibrosis and cirrhosis induced by $CCl_4$, and the ingredients could be a promising candidate for the prevention and treatment of hepatic disorders.

• KSBB Journal
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• 제24권2호
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• pp.170-176
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• 2009

한국산 장류콩 종자로부터 최종적으로 Sephadex G-100상에서 분리한 lectin의 분자량, 적혈구 응집력, 온도, 열 안정성, 및 pH의 생화학적 특성을 조사하였다. 분리된 lectin을 SDS-PAGE한 결과, 분자량이 32 kDa와 22 kDa인 2개의 band가 나타났으며, Native PAGE에 의해서 108 kDa의 tetramer임을 알 수 있었다. Lectin은 trypsin을 처리한 사람의 A, B, AB, O 혈액과 쥐 혈액에서 혈구응집 반응이 일어나지 않았으나 토끼에서는 응집 반응이 일어났으며, trypsin을 처리하지 않은 모든 혈액에서는 응집반응이 일어나지 않았다. 이 lectin은 최적온도가 20-$50^{\circ}C$이며, 10-$60^{\circ}C$ 에서 열 안정성을 보였다. 최적 pH는 7.2로서 6.2 이하와 8.0 이상에서는 활성이 상설되었다.

• 한국임상수의학회지
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• 제28권2호
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• pp.219-224
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• 2011

서로 다른 고도에서 사육하고 있는 2-3세령의 수컷, 건강한 비육 한우를 세 그룹(600 m, n=50; 200-400 m, n=58; 0 m n=49)으로 나누어 혈액 및 혈청검사 그리고 면역항체가 검사를 실시 하였다. 고도를 제외한 일반 사육 환경과 식이 및 축사의 형태는 매우 비슷한 조건이였으며 질병이 확인 된 개체는 통계에서 제외 하였다. 경정맥에서 채취한 혈액은 일반혈액검사 중 WBC, RBC, Hb, PCV 를, 혈청화학검사 중 AST, LDH, TBL를 집중적으로 비교 분석하였으며 또한 면역학적 검사를 실시하였다. 고도가 높은 그룹이 낮은 그룹에 비하여 CBC에서는 조금 높게 나타났으며, 혈청검사에서는 평지에서 사육된 한우가 조금 높게 나왔다. 그러나 모두가 정상범위를 벗어나거나 통계적으로 유의한 수준의 차이를 보이지 않았다. 이러한 결과는 지금까지 알려진 대부분의 연구결과와는 차이가 있는 것이다. 본 연구와 지금까지의 알려진 연구와의 이러한 차이는 과거의 연구들은 고도의 차이가 현저한 경우가 대부분이었으나 본 연구에서 조사한 지역은 서로 고도가 비슷한 점에서 생긴 결과라고 여겨지며, 특히 고도가 600m 이하인 지형에서는 서로 큰 영향을 받지 않는 것으로 생각된다. 따라서 한우의 경우 목장 시설의 건축시 지형에 따른 어려움과 주변에서 들여오는 조사료의 수송에 따른 경제적 불리, 축산폐수 처리는 물론 퇴비의 활용 등 전반적인 목장 관리 면에서 고도가 높은 산지 보다는 평지가 권장된다.

• 한국산학기술학회논문지
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• 제21권12호
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• pp.395-400
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• 2020

해부학적, 생리학적으로 인간과 유사한 특성을 지닌 돼지를 이용한 실험은 의학적 분야에서 폭넓게 이용되고 있다. 돼지에서 혈액의 일반적인 성상과 이화학적 수치는 의학적 연구 및 수의학적 치료에서도 중요한 부분으로 인정되고 있으나, 형질전환 돼지에 대한 연구는 미비한 실정이다. 따라서, 본 연구에서는 htPA 형질전환 돼지의 혈액을 이용한 일반성상 및 이화학적 성상을 비교 분석하여 형질전환 돼지에 대한 기초 자료로 활용하고자 조사하였다. 일반돼지 7(LY)두와 형질전환 돼지 8(LY)두의 혈액을 각각 분석하였다. 혈액의 일반 성상은 16종을 분석하였으며, 혈청을 이용한 이화학 분석의 경우 15종 항목을 조사하였다. 그 결과 혈액의 일반 성상 분석에서는 적혈구(RBC), 평균적혈구 혈색소량(MCH)과 임파구(LYM)에서 두 그룹간 유의적 차이를 나타내었다. 이화학적 성상 분석에서는 혈중뇨소질소(BUN), 총단백질(TP), 콜레스테롤(CHOL), (ALT), 크레아틴(CREA), 감마글루타밀전이효소(GGT), 글로빈(GOB) 그리고 아밀라아제(AMYL)가 두 그룹간 유의적 차이를 나타내었다. 앞으로 지속적인 형질전환 돼지에 대한 생체정보를 조사함으로써, 기초 자료로 이용할 수 있을 뿐만 아니라, 더 나아가 의학적 연구 분야에 적용 시 참고할 수 있을 것으로 여겨진다.

• Journal of Nutrition and Health
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• 제35권5호
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• pp.512-523
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• 2002

The objective of this study was to investigate the nutritional status, biochemical parameters, lipid and electrolytes concentrations of the enteral nutrition patients according to the duration of enteral nutrition. Eighteen neurosurgery patients in the intensive care unit (ICU) at K University Hospital were subjected in this study. The duration of enteral nutrition was classified into under or over six month of period. Anthropometric, biochemical, clinical, and dietary assessments were performed. Patients' intakes of energy and protein were insufficient, from 82% to 95% of their requirements. Mid-arm muscle circumference (MAMC) and mid-am muscle area (MAMA) were significantly lower in patients over six months of enteral nutrition than those in patients under six months. The subjects were malnourished as indicated by nutrition-related parameters such as hemoglobin, albumin, total lymphocyte count (TLC), tricep skinfold thickness (TSF), mid-arm circumference (MAC), MAMC, and MAMA. Serum chloride level of the patients eve, six months of enteral nutrition was lower (94.7 $\pm$ 3.4 mmo1/1) significantly as compared to that of patients (99.3 $\pm$ 3.5 mmol/ 1) under six months. Urinary sodium and chloride levels were lower in the longer time of enteral nutrition patients than those of shorter period of enteral nutrition patients (p < .05). While serum phospholipid level was higher in the patients over six months of enteral nutrition, other blood biochemical parameters and electrolyte concentrations did not show any differences with the duration of enteral nutrition. Neurosurgery patients in the ICU undergoing long-term enteral nutrition tube-feeding were malnourished and had a variety of metabolic complications. The duration of enteral nutrition could affect the patients' nutritional status, biochemical parameters, and electrolytes balance. The patients who require nutritional support over an extended time need the continuous follow-up care and monitoring by the nutrition support team for laboratory, clinical, and nutritional assessments.

• 대한지역사회영양학회지
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• 제6권5호
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• pp.765-772
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• 2001

To delineate the relationship between the nutrient intake from diet and the serum biochemical markers of bone metabolism, 56 postmenopausal women of 50 to 77 years of age were recruited. The biochemical markers including osteocalcin, calcium, phosphorus, estradiol and free testosterone were measured in fasting blood. Bone mineral density(BMD) was measured also by dual energy X-ray absorptiometry, and the nutrient intake of earth individual subject was estimated by 24-hour recall of 3 days. The age of the subjects was 64.8 $\pm$ 7.7 years, and the BMDs of the subject were 0.86 $\pm$ 0.26g/$cm^2$(Lumbar spine), 0.60 $\pm$ 0.10g/$cm^2$ (Femoral neck), 0.49 $\pm$ 0.10g/$cm^2$(Trochanter), and 0.41 $\pm$ 0.14g/$cm^2$(Ward's triangle). There were no significant differences among age and nutrient intake level groups due to the small sample size. The biochemical markers showed certain degree of relationship with nutrient intake levels. The results were compared among 3 groups with different nutrient intake level classified by the percentage of Recommended Daily Allowances(RDA) for Koreans as follows low < 75% RDA, 75% RDA $\leq$ adequate < 125% RDA, high $\geq$ 125% RDA. The low energy and low riboflavin groups showed significantly higher serum osteocalcin levels than those of the high intake groups(p < 0.05). On the other hand, there was a trend for serum Ca level to be higher with high nutrient intake. In this case, protein and thiamin were the only nutrients that reached a statistical significance(p < 0.05). And the groups with low intake for protein and Ca showed significantly lower serum free testosterone levels than that of other intake groups(p < 0.05). This study suggests an important role of nutrient intake levels on blood biochemical markers of bone metabolism.

• Journal of Community Nutrition
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• 제7권3호
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• pp.130-134
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• 2005

This study was conducted by surveying 616 male workers living in Ulsan City regarding their health status based on lifestyles such as alcohol consumption, smoking and exercising as well as physical measurements and biochemical tests. The average height, weight and BMI(body mass index, $kg/m^2$) of the subjects was 170.9cm, 70.2kg and 24.2, respectively. The rate of drinking was $80.9\%$ and the rate of smoking was $53.4\%$. Seventy four percent of subjects responded that they exercise regularly. The results of the blood biochemical tests revealed that the average hemoglobin concentration was 14.7g/dl, and the levels of GPT(glutamic pyruvic transaminase) and GOT (glutamic oxaloacetic transaminase) were 32.74unit/l, 26.99 unit/l, respectively. The average hemoglobin concentration for the subjects aged in the 50s was 14.39g/dl, which was significantly lower than those in the 20s(14.81g/dl), 30s(14.69g/dl) and 40s(14.73g/dl). The blood glucose level and the cholesterol level also increased with age. Also investigated was the blood pressure of the subjects increased with age,. and there was a significant increase(p < 0.05) for the subjects in the 50s compared to those in the 20s. The frequency of alcoholic beverages was significantly correlated with systolic/ diastolic blood pressure(p < 0.05) and $\gamma-GTP(gamma\;glutamyl\; transpeptidase)$(p<0.01). The duration of smoking showed a negative correlation(p < 0.05) with the hemoglobin and positive correlations with diastolic blood pressure, cholesterol and $\gamma-GTP(p<0.01)$. The study shows that blood pressure, blood glucose level, cholesterol level, GOT, GPT and $\gamma­GTP$ level, increase with age, which indicates higher possibility of degenerative diseases, calling for nutritional education in terms of advisable lifestyles regarding eating habits, alcohol consumption, smoking and regular exercise.

• Asian-Australasian Journal of Animal Sciences
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• 제24권12호
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• pp.1681-1689
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• 2011

The beneficial effects of tea catechins (TCs) are related not only to their antioxidant potential but also to the improvement of animal meat quality. In this study, we assessed the effects of dietary TC supplementation on plasma biochemical parameters, hormone responses, and glutathione redox status in goats. Forty Liuyang goats were randomly divided into four equal groups (10 animals/group) that were assigned to four experimental diets with TC supplementation at 4 levels (0, 2,000, 3,000 or 4,000 mg TC/kg DM feed). After a 60-day feeding trial, all goats were slaughtered and sampled. Dietary TC treatment had no significant effect on blood biochemical parameters, however, low-density lipoprotein cholesterol (p<0.001), triglyceride (p<0.01), plasma urea nitrogen (p<0.01), and glucose (p<0.001) decreased and total protein (p<0.01) and albumin (p<0.05) increased with the feeding time extension, and day 20 was the turning point for most of changes. Interactions were found in glutathione (p<0.001) and the ratio of reduced and oxidized glutathione (p<0.05) in whole blood between treatment and feeding time. Oxidized glutathione in blood was reduced (p<0.05) by 2,000 mg TC/kg feed supplementation, and a similar result was observed in longissimus dorsi muscle. Though plasma glutathione peroxidase (p<0.01) and glutathione reductase (p<0.05) activities were affected by treatment and feeding time interactions, and glutathione S-transferases activity increased with feeding day extension, no changed values appeared in longissimus dorsi muscle. In conclusion, dietary TC supplementation affected the concentrations of some blood metabolites and accelerated GSH depletion in the blood of goats. In terms of less high-density lipoprotein cholesterol, the highest insulin and IGF-I concentrations, the highest ratio of reduced and oxidized glutathione in plasma, the dosage of 2,000 mg TC/kg feed might be desirable for growing goats to prevent glutathione depletion and keep normal physiological metabolism.

• 대한지역사회영양학회지
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• 제11권3호
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• pp.346-360
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• 2006

This study was conducted to evaluate the effects of home-visiting nutrition education for the elderly with high fasting glucose level in an urban community. The study subjects were 40 elderly people, whose information on general characteristics, anthropometric measurement, biochemical indices, nutrition knowledge, nutrition attitude, dietary habit, food intake and nutrient intake were obtained at baseline. The education group received 6 weekly visits of home-visiting nutrition education from 15 March to 25 April 2004. In the baseline-survey, the education group and non-education group showed no differences in their general characteristics, health-related characteristics, anthropometric measurements, biochemical indices, nutrition knowledge, nutrition attitudes, dietary habits, and food and nutrient intake levels. The difference of mean change of nutrition knowledge, nutrition attitude and dietary habit after home-visiting nutrition education had been studied. The nutrition knowledge score increased by 1.4 in the education group; however, those in the non-education group which increased by 0.4, thus, the differences of mean change were statistically significant (p < 0.05). The nutrition attitude score increased by 1.2 in the education group; however, those in the non-education group decreased by 0.4, thus, the differences of mean change were statistically significant (p < 0.01). The dietary habit score increased by 1.7 in the education group; however, those in the non-education group decreased by 2.8, thus, the differences of mean change were statistically significant (p < 0.01). The difference of mean change of anthropometric indices and biochemical indices in education group and the non-education group was not significant. Looking over the zcereals and their products, vegetables, seaweeds, meats and their products, and fish than the non-education group. The MAR increased by 0.06 in the education group; however, that in the non-education group increased by 0.01, thus, the differences of mean change were statistically significant (p < 0.05). The differences of mean change of fasting blood glucose and biochemical indices after home-visiting nutrition education were studied. Fasting blood glucose decreased by 7.6 mg/dL in the education group; however, in the non-education group which increased by 0.4 mg/dL, the difference of mean change was not significant (p = 0.051). The above findings suggest that home visiting nutrition education increases the nutrition knowledge and nutrition attitude, as well as, it is effective to change dietary habits. If the education period is extended, not fasting blood glucose improvement was insignificant, but fasting blood glucose improvement ability could be found by changing dietary habits.

• 한국가금학회지
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• 제42권4호
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• pp.307-314
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• 2015

본 연구는 한방생약부산물을 이용한 육계의 사료첨가제를 개발하기 위해 대조군(CON), HM1(한방생약부산물 0.15%), HM2(한방생약부산물 0.3%) 및 HM3(한방생약부산물 0.5%) 등 4개군에 육계 96수를 완전임의 배치하여 생산성, 장내 미생물 균총, 혈액 생화학적 지표, 면역글로블린 및 백혈구 성상의 변화에 대하여 조사하였다. 한방생약부산물 0.15% 첨가군(HM1)에서 대조군에 비해 육계전기(3~21일령) 사료요구율이 유의적(p<0.05)으로 개선되었으나, 전 기간에서는 차이가 없었다. 장내 소화물의 E. coli 및 Lactobacili 총 균수에서도 한방생약부산물 급여는 영향을 미치지 않았다. 혈액 생화학적 지표에서는 한방생약부산물 급여 시 AST 및 glucose 수준을 유의하게(p<0.05) 감소시켰다. 면역글로불린(IgG 및 IgA)과 백혈구 감별계수에서는 처리에 따른 차이는 없었다. 결론적으로 육계에서 0.15~0.3% 수준의 한방생약 부산물 첨가는 육계전기에 사료이용성 및 간 조직의 기능을 개선하는 효과가 있는 것으로 사료된다.