• Journal of Life Science
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• v.5 no.2
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• pp.63-69
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• 1995

Cockroach antigen have been known as a cause of allergic disease. German ockroach(Blattella germanica L.) was chosen because it has the highest distribution range and poulation density. To identify the common and specific antigens of adult and larval stage of german cockroach, we made monoclonal antibodies which were confirmen by SDS-PAGE and EITB. Anti-B. germanica antibody producing hybridomas were 24 among the total 960wells. Only 4 hybridomas did not have cross reaction to other species of cockroach and hluse dust mites(Dermatophagodies farinae and D. pteronyssius). SDS-PAGE revealed about 20 bands from 90Kd to 15Kd to 15Kd. ETB showed specific antigens a6 60, 72 and 82Kd which were experimented by the culture supernatant of 4 selected hybridomas. Especially 60Kd coincided with a band of immunized mouse sera.

• Journal of Life Science
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• v.5 no.2
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• pp.7-7
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• 1995

Cockroach antigen have been known as a cause of allergic disease. German ockroach(Blattella germanica L.) was chosen because it has the highest distribution range and poulation density. To identify the common and specific antigens of adult and larval stage of german cockroach, we made monoclonal antibodies which were confirmen by SDS-PAGE and EITB. Anti-B. germanica antibody producing hybridomas were 24 among the total 960wells. Only 4 hybridomas did not have cross reaction to other species of cockroach and hluse dust mites(Dermatophagodies farinae and D. pteronyssius). SDS-PAGE revealed about 20 bands from 90Kd to 15Kd to 15Kd. ETB showed specific antigens a6 60, 72 and 82Kd which were experimented by the culture supernatant of 4 selected hybridomas. Especially 60Kd coincided with a band of immunized mouse sera.

• Parasites, Hosts and Diseases
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• v.43 no.4 s.136
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• pp.149-156
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• 2005

Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About $42\%$ (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling path-ways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these rep-resent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.

• Korean journal of applied entomology
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• v.32 no.1
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• pp.24-29
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• 1993

Experiments were conducted to study effects of different bioassay methods on toxicity to the German cockroach (Blattella germanica L.) ith six insecticides. For male adults, by dry film method, the {TEX}$LC_{50}${/TEX} value of chlorpyrifos. fenvalerate, propoxur, permethin, DDVP, and hydramethylnon was 1.79, 1.87, 3.04, 4.37, 32.72, and 270.81 ppm, respectively and the same tendency was shown in the female. For male adults, by topical micro-application method, the {TEX}$LD_{50}${/TEX} value of DDVP and fenvalerate, chlorpyrifls, permethrin, propoxur, hydramethylnon was 2.63, 3.79, 4.51, 6.73, 44.99 $\mu\textrm{g}$/g, respectively and the same tendency was shown in the female. From the diet toxicity method against male adults, the CT value (Concentration%$\times${TEX}$LT_{50}${/TEX}) showed that chlorpyrifos was rapid effective and hydramethylnon was residual effective.

• Korean journal of applied entomology
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• v.32 no.2
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• pp.129-133
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• 1993

The German cockroach (Blattella germanical L.) populations were successively selected with chlorpyrifos and permethrin for xis generations. The resulting resistant strains, $R_{chlorpyrifos}$(Rc) and $R_{permethrin}$(Rp) were observed resistant development and the cross-resistance. The result indicated that the values of $LC_{50}$ were increased 3.32 and 2.10 times from the susceptible strain, respectively. In the Rc strains, the values of the $LC_{50}$ of fenvalerate, propoxur, permethrin were to 3.89, 2.97, and 1.31 times higher than that of the suseptible, respectively. In the Rp strains, the values of the $LC_{50}$ of fenvalerate, chlorpyrifos, and propoxur showed 5.23, 1.42, and 1.80 times cross-resistance.

• Korean journal of applied entomology
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• v.32 no.3
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• pp.265-270
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• 1993

The German cockroach(Blattelia germanica) population~ were successIVely selected with ch\orpyrifos and permethrin during the six generations. The resulting resistant $R_{chtorpenfos}$(Rc) and $R_{permethnn}$(Rp) stra.ins were studied to investigate the esterase activity by spectrophotometer, filter parper test, and electrophoresis. Esterase-$\alpha$ activities by filter paper test showed 2.65 and LBZ times higher in the Rc and Rp strains than the susceptible strain, respectively. ln the spectrophoLometer method, the esterase activit18s to $\alpha$-and $\beta$-naphthyl acetate were increased 2.34 and 5.28 times in the Rc than susceptible strain, and 1.48 and 2.92 times in the Rp Limn susceptible stram, respectlvely. Zymogram patterns of eslerase isozyme by agarose gel electrophoresis showed totally five bands. The Rc and Rp strains showed two additive bands as, Est-2 and Est-3, which were not shown in the susceptible strain. but the Rp strain dId not show Est-5 bands which was COlumon in the Rc and susceptible strams.

• Korean journal of applied entomology
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• v.43 no.3 s.136
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• pp.241-247
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• 2004

Susceptibility of Blattella germanica male adults to 61 commercial insecticides was evaluated by diet dipping method. Among them three insecticides of dichlorvos, fenitrothion, and fipronil showed over $90\%$ mortality. The insect was more susceptible to dichlorvos and fenitrothion in filter paper contact method than in diet dipping one, but fipronil was vice versa. Toxicities of the three chemicals were evaluated by application parts of the such as head, thorax, abdomen, and legs. Dichlorvos and fenitrothion were more toxic when applied to head, and flpronil was thorax. $LT_{50}$ values by diet dipping method showed that fenitrothion and fipronil acted more rapidly than boric acid and hydramethylnon. The values of the former two were 1.05 and 0.98 days, and those of the latter two were 3.92 and 2.26 days, respectively.

• Parasites, Hosts and Diseases
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• v.43 no.3 s.135
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• pp.101-110
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• 2005

In this study, the trypsin gene (bgtryp-1) from the German cockroach, Blattella germanica, was cloned via the immunoscreening of patients with allergies to cockroaches. Nucleotide sequence analysis predicted an 863 bp open reading frame which encodes for 257 amino acids. The deduced amino acid sequence exhibited $42-57\%$ homology with the serine protease from dust mites, and consisted of a conserved catalytic domain (GOSGGPLV). bgtryp-1 was determined by both Northern and Southern analysis to be a 0.9 kb, single-copy gene. SDS-PAGE and Western blotting analyses of the recombinant protein (Bgtryp-1) over-expressed in Escherichia coli revealed that the molecular mass of the expressed protein was 35 kDa, and the expressed protein was capable of reacting with the sera of cock-roach allergy patients. We also discussed the possibility that trypsin excreted by the digestive system of the German cockroach not only functions as an allergen, but also may perform a vital role in the activation of PAR-2.

• Journal of environmental and Sanitary engineering
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• v.8 no.1
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• pp.129-138
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• 1993

The marked spread of the cockraches of recent years has raised a great social problem in urban areas. The cockroach have to remove1 because transmit a disease to human as pest insect, but particulars are not yet reported on biological control agent for the cockroach removal. This study was tried for the first time on biological control for the cockroach removal. The obtained results were as follows : 1. The isolated were spore-forming bacillus 1098 strain in soil. The No. 109(TH 109) strain of the among spore-forming bacillus was showed the poisonous against Cockroach. 2. The biological characteristics and flagella antigenicity of the strain is similar to Bacillus thur-ingiensis subsp. indiana. 3. TH 109 strain have the delta-endotoxin of cuboid shap. 4. This delta-endotoxin of product by TH 109 strain was toxic to the cockroach(Blattella gemzanica. L).

• Korean journal of applied entomology
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• v.44 no.1 s.138
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• pp.37-41
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• 2005

Essential oil of five plants were screened for fumigation toxicity against Blattella germanica male adults. Among them, coriander oil showed $100\%$ fumigation toxicity against B. germanica male adults at 20 and $10{\mu}{\ell}/{\ell}$ (air) dose, respectively. Through the constituent analysis using GC and GC/MS, we confirmed that main constituents of coriander oil were ${\alpha}$-pinene ($6.1\%$), camphene ($1.1\%$), ${\beta}$-pinene ($0.5\%$), ${\beta}$-myrcene ($0.8\%$), limonene ($2.5\%$), ${\gamma}$-terpinene ($4.5\%$), ${\rho}$-cymene ($1.8\%$), ${\alpha}$-terpinolene ($0.5\%$), camphor ($4.9\%$), linalool ($70.5\%$), ${\alpha}$-tepineol ($0.7\%$), geranyl acetate ($2.8\%$) and geraniol ($1.4\%$). Among them, ${\gamma}$-terpinene and geranyl acetate showed $100\%$ fumigation toxicity at $10{\mu}{\ell}/{\ell}$ (air) dose, respectively. It can be concluded that coriander oil is potential control agents against B. germanica.