• 한국동물위생학회지
• /
• 제40권1호
• /
• pp.15-20
• /
• 2017

A two-tube reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was designed for the rapid visual detection of the M gene of all subtypes of avian influenza virus (AIV) and the H5 gene of the H5 subtype of highly pathogenic AIV (HPAIV). The reaction carried out in two tubes in a single step at $58^{\circ}C$ for 40 min, and the assay results could be visually detected by using hydroxynaphthol blue dye. Using M or H5 gene-specific primers, the assay successfully detected all subtypes or H5 subtypes of AIVs, including the Korean representative H5N1 and H5N8 HPAIVs. The detection limit of the assay was approximately $10^{2.0}$ $EID_{50}/reaction$ for the M and H5 genes of H5N1 HPAIV, respectively, and was more sensitive than that of previously reported RT-LAMP and comparable to that of real-time RT-PCR. These results suggest that the present RT-LAMP assay, with its high specificity, sensitivity, and simplicity, will be a useful diagnostic tool for surveillance of currently circulating H5 HPAIVs and other subtypes of AIV in bird population, even in under-equipped laboratories.

• 한국컴퓨터정보학회논문지
• /
• 제11권5호
• /
• pp.251-258
• /
• 2006

개인 정보의 탈취에 필요한 악성코드는 Phishing, Pharming메일, VoIP서비스를 이용하는 Vishing, 모바일 금융을 위장한 SMiShing 등에 이용되어진다. 악성코드의 삭제나 치료는 앤티바이러스나 스파이웨어 제거 프로그램을 사용한다. 그런데 DLL Injection 기법을 이용하여 기생 동작하는 악성코드는 윈도우 운영체제에서 반드시 실행되어야 하는 프로세스인 Isass.exe, winlogon.exe, csrss.exe와 연계되어 있어 치료가 되지 않는다. 사용자가 바이러스의 치료를 위하여 임의로 프로세스를 강제 종료하려 한다면, 운영체제 시스템 전체가 리부팅이 발생하거나 블루 스크린이 발생한다. 본 논문에서는 치명적인 결과를 발생하는 DLL Injection 기법을 이용하는 악성코드를 치료하는 새로운 치료방법을 제안한다. 새로운 치료를 위한 Thread에 종료함수를 임의로 삽입하고 Thread를 제어하여 DLL의 동작을 종료하고, 종료함수가 가지는 Thread를 다시 Injection 한 후 KILL DLL 클릭하여 삭제한다. 본 논문에서 실험을 한 치료방법과 해결하는 방안은 컴퓨터 바이러스의 대한 획기적인 차원의 연구가 될 것이며 경제와 금융사회의 유비쿼터스 보안을 강화하는 초석이 될 것이다.

• 농업과학연구
• /
• 제48권2호
• /
• pp.231-239
• /
• 2021

This study evaluated the effect of microwave-dried black soldier fly (Hermetia illucens) larvae meal (HILM) as a dietary protein source for broiler diets. A total of 250 male broilers were randomly allocated to five dietary treatment groups (10 replicates and 5 birds·pen^-1) which were respectively fed the following: a control diet (HI0) and four experimental diets in which soybean meal was replaced with 2 (HI2), 4, 6, and 8% HILM. At the end of the study (35 d), blood samples were collected from 10 randomly selected broilers (1 bird per pen) to determine serum parameters. Then, the broilers were slaughtered to determine volatile fatty acid (VFA) profiles in cecal digesta. The average daily gain and average daily feed intake linearly decreased (p < 0.01), and the feed conversion ratio linearly increased (p < 0.05) according to the inclusion level of HILM; however, there were no significant differences between the HI0 and HI2 groups. Dietary HILM increased (p < 0.01) the total VFAs in cecal digesta and decreased (p < 0.05) the triglyceride level in the blood. The broilers fed HILM had lower (p < 0.01) serum phosphorus levels; dietary HILM increased (p < 0.05) the serum calcium level. The total VFAs in cecal digesta were positively influenced by the dietary microwave-dried HILM. However, a low inclusion level (2%) of HILM in broiler diets is proper in terms of growth performance and health.

• Journal of Animal Science and Technology
• /
• 제65권3호
• /
• pp.638-651
• /
• 2023

The objective of this study was to quantitatively estimate the level of grazing area damage in outdoor free-range pig production using a Unmanned Aerial Vehicles (UAV) with an RGB image sensor. Ten corn field images were captured by a UAV over approximately two weeks, during which gestating sows were allowed to graze freely on the corn field measuring 100 × 50 m². The images were corrected to a bird's-eye view, and then divided into 32 segments and sequentially inputted into the YOLOv4 detector to detect the corn images according to their condition. The 43 raw training images selected randomly out of 320 segmented images were flipped to create 86 images, and then these images were further augmented by rotating them in 5-degree increments to create a total of 6,192 images. The increased 6,192 images are further augmented by applying three random color transformations to each image, resulting in 24,768 datasets. The occupancy rate of corn in the field was estimated efficiently using You Only Look Once (YOLO). As of the first day of observation (day 2), it was evident that almost all the corn had disappeared by the ninth day. When grazing 20 sows in a 50 × 100 m² cornfield (250 m²/sow), it appears that the animals should be rotated to other grazing areas to protect the cover crop after at least five days. In agricultural technology, most of the research using machine and deep learning is related to the detection of fruits and pests, and research on other application fields is needed. In addition, large-scale image data collected by experts in the field are required as training data to apply deep learning. If the data required for deep learning is insufficient, a large number of data augmentation is required.

• 환경영향평가
• /
• 제6권1호
• /
• pp.77-92
• /
• 1997

덕진공원의 생물상을 조사하고 환경 및 생물의 서식 상태를 개선한 수 있는 기초적인 대책을 수립하기 위하여 1996년 6월에 조사를 실시하였다. 조사는 식물상, 플랑크톤 조사, 곤충상, 척추동물상(어류, 양서류, 파충류, 조류, 포유류)으로 구분하여 실시하였다. 조사결과, 식물상은 모두 49과 84속 83총 1품종 11변종으로 확인되었다. 호수에는 연꽃 Nelembo nucifera과 개구리밥 Spirodela polyrhiza이 우점종을 차지하였고, 수질개선의 측면에서 동절기에 이들의 제거가 요구된다. 덕진공원 내에 플랑크톤은 6강 10목 18과 40속이 출현하였고 이중 부영양화 현상을 일으키는 Anacystis(=Microcystis)와 Anabaena가 출현하였다. 공원내에서 출현하는 곤충은 11목 30과 41종으로 확인되었고 보다 많은 곤충이 모이게 하기 위해서 다양한 화초류을 심는 것이 필요하다고 본다. 한편 어류는 7종, 양서류는 2종, 파충류는 3종, 조류는 7종 및 포유류는 1종이 관찰되었다. 이중에서 황소개구리와 청거북과 같은 외래도입종을 제거하기 위해서는 특별한 관심이 지속적으로 요구된다.

• 한국환경생태학회지
• /
• 제25권2호
• /
• pp.132-144
• /
• 2011

본 연구는 덕유산국립공원의 향적봉지역(A)과 백련사지역(B) 및 안성지역(C)을 대상으로 2010년 4월부터 9월까지 산림환경과 조류군집 특성을 조사하였다. 향적봉지역은 신갈나무 등의 낙엽활엽수림과 주목, 구상나무 등의 아고산대침엽수림과 원추리, 철쭉 등의 초지가 형성되어있고, 백련사지역과 안성지역은 대부분 낙엽활엽수림으로 구성되고 계곡부는 들메나무 군락이 형성되어 있다. 교목의 흉고직경분포는 11~20cm 경급이 가장 많았으며, 30cm이상 대경목 비율은 백련사지역에서 가장 높았고, 향적봉지역이 가장 낮았다. 또한 엽층별 피도량은 향적봉지역은 하층부 피도량이 매우 높았고, 백련사지역은 8~12m의 중층부 피도량이 높았으며, 안성지역은 18m이상 상층부 피도량이 높게 나타났다. 덕유산국립공원에서 조사기간동안 관찰된 조류는 총 53종이었다. 각 지역별 종수와 밀도는 각각 향적봉지역이 25종 45.20 Ind./km, 백련사지역이 50종 58.63 Ind./km, 안성지역 35종 66.89 Ind./km로 백련사지역의 종수가 가장 많았고, 밀도는 안성지역에서 높았다. 우점종은 향적봉지역이 휘파람새를 비롯한 초지와 관목에 서식하는 종들이 우점하였고, 백련사지역과 안성지역은 오목눈이를 비롯한 수관층에 서식하는 종들이 우점하였다. 길드 구조는 모든 지역에서 관목층 영소길드와 채이길드 종이 높게 나타났고, 수동 영소길드와 공중 채이길드 종의 비율이 낮게 나타났다. 이는 흉고직경 30cm이상의 대경목 비율이 낮아 수동 영소길드 종들의 둥지목이 부족한 결과로 생각된다.

• 한국환경과학회지
• /
• 제17권5호
• /
• pp.537-546
• /
• 2008

The experiment was conducted to determine the effect of the mixed herbal medicine for the substitution of antibiotics on the performance of laying hens. Day old hyline 1,500 layer chicks were randomly assigned to 4 treatments. Control were 600 and three each treatment was 300. Eggs were collected at every weeks for measuring egg production and week 26, 27, 28 for chemical analyses. Chemical analyses were done for fatty acid profile of egg yolks, amino acid, antibiotics residue in eggs(collect eggs after supplying OTC 21ppm/bird/day to control for four day at 26, 27, 28) during the laying period($19{\sim}77wk$). On $0{\sim}4$ week, T-3 showed higher feed intake than those fed the other diets and on $5{\sim}13$ week, T-2 showed highest feed intake among treatments. After birds were moved ($14{\sim}18$ wk) CT-3 showed highest, but for overall rearing period, there was not significantly different among treatments. In conclusion the mixed herbal medicine did not tended to influence palatability to rearing birds. Egg quality(egg yolk color, haugh unit, eggshell breaking strength) showed no difference among treatments. Ratio of unsaturated fatty acid (mg/100g)/saturated fatty acid (mg/100g) was not difference among treatments, but linolenic acid and docosahexaenoic acid(DHA) of the mixed herbal medicine treatments showed higher then control. The antibiotics residue of CT, T treatments egg was not detected and control was not detected or below allowance. In conclusion the mixed herbal medicine can be possible to feed laying hen without antibiotics.

• Parasites, Hosts and Diseases
• /
• 제52권5호
• /
• pp.513-520
• /
• 2014

Three echinostome species, i.e., Patagifer bilobus, Petasiger neocomense, and Saakotrema metatestis, are newly recorded in the trematode fauna of the Republic of Korea. They were recovered from 3 species of migratory birds (Platalea minor, Podiceps cristatus, and Egretta garzetta), which were donated by the Wildlife Center of Chungbuk (WCC) and the Conservation Genome Resource Bank for Korean Wildlife (CGRB). Only 1 P. bilobus specimen was recovered from the intestine of a black-faced spoonbill (P. minor), and characterized by the bilobed head crown with a deep dorsal incision and 54 collar spines. Twenty P. neocomense were recovered from the intestine of a great crested grebe (P. cristatus), and they had a well-developed head crown with 19 spines and 2 testes obliquely located at the posterior middle of the body. Total 70 S. metatestis were collected from the bursa of Fabricius of 1 little egret (E. garzetta). It is characterized by stout tegumental spines covered in the entire leaf-shaped body, posterior extension of the uterus, presence of the uroproct and a well-developed head crown with 12 pairs of collar spines on each side. By the present study, these 3 echinostome species are newly added to the trematode fauna in Korea.

• 대한수의학회지
• /
• 제52권4호
• /
• pp.285-288
• /
• 2012

Salmonella I 4,[5],12:i:- was a monophasic variant of Salmonella (S.) Typhimurium and notorious for re-emerging candidate which would replace S. Typhimurium DT104 for antibiotic resistance. Recently, isolation rate was increased on human and industrial animals but there was no case in domestic animals but human in Korea. This was first isolation case from domestic animals in Korea. The five isolates from feces of duck (n = 3), chicken (n = 1), and wild bird (n = 1) showed antibiotic resistance against cephems and aminoglycosides. These means that the spread of emerging bacterial pathogens to domestic animals and the need of systemic management for Salmonella I 4,[5],12:i:-.

• 한국환경과학회지
• /
• 제24권1호
• /
• pp.133-150
• /
• 2015

Wind profiler provides vertical profiles of three-dimensional wind vectors with high spatiotemporal resolution. The wind vectors is useful to analyze severe weather phenomena and to validate the various products from numerical weather prediction model. However, the wind measurements are not immune to ground clutter, bird, insect, and aircraft. Therefore, quality of wind vectors from wind profiler must be quantitatively evaluated prior to its application. In this study, wind vectors from UHF wind profiler at Ganwon Regional Meteorological Administration was quantitatively evaluated using 27 radiosonde measurements that were launched every two or three hours according to rainfall intensity during Intensive Observation Period (IOP) from June to July 2013. In comparison between two measurements, wind vectors from wind profiler was relatively underestimated. In addition, the accuracy and quality of wind vectors from wind profiler decrease with increasing beam height. The accuracy and quality of the wind vectors for rainy periods during IOP were higher than for the clear-air measurements. The moderate rainfall intensity lead to multi-peaks in Doppler spectrum. It results in overestimation of vertical air motion, whereas wind vectors from wind profilers shows good agreement with those from radiosonde measurements for light rainfall intensity.