• 제목/요약/키워드: Biomolecule

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Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: Cloning, Sequencing, and Characterization

  • Basnet, Devi Bahadur;Oh, Tae-Jin;Vu, Thi Thu Hang;Sthapit, Basundhara;Liou, Kwangkyoung;Lee, Hei Chan;Yoo, Jin-Cheol;Sohng, Jae Kyung
    • Molecules and Cells
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    • 제22권2호
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    • pp.154-162
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    • 2006
  • The entire gene cluster involved in the biosynthesis of angucyclines Sch 47554 and Sch 47555 was cloned, sequenced, and characterized. Analysis of the nucleotide sequence of genomic DNA spanning 77.5-kb revealed a total of 55 open reading frames, and the deduced products exhibited strong sequence similarities to type II polyketide synthases, deoxysugar biosynthetic enzymes, and a variety of accessory enzymes. The involvement of this gene cluster in the pathway of Sch 47554 and Sch 47555 was confirmed by genetic inactivation of the aromatase, including a portion of the ketoreductase, which was disrupted by inserting the thiostrepton gene.