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Angucyclines Sch 47554 and Sch 47555 from Streptomyces sp. SCC-2136: Cloning, Sequencing, and Characterization  

Basnet, Devi Bahadur (Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, Sun Moon University)
Oh, Tae-Jin (Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, Sun Moon University)
Vu, Thi Thu Hang (Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, Sun Moon University)
Sthapit, Basundhara (Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, Sun Moon University)
Liou, Kwangkyoung (Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, Sun Moon University)
Lee, Hei Chan (Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, Sun Moon University)
Yoo, Jin-Cheol (Department of Pharmacy, Chosun University)
Sohng, Jae Kyung (Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, Sun Moon University)
Publication Information
Molecules and Cells / v.22, no.2, 2006 , pp. 154-162 More about this Journal
Abstract
The entire gene cluster involved in the biosynthesis of angucyclines Sch 47554 and Sch 47555 was cloned, sequenced, and characterized. Analysis of the nucleotide sequence of genomic DNA spanning 77.5-kb revealed a total of 55 open reading frames, and the deduced products exhibited strong sequence similarities to type II polyketide synthases, deoxysugar biosynthetic enzymes, and a variety of accessory enzymes. The involvement of this gene cluster in the pathway of Sch 47554 and Sch 47555 was confirmed by genetic inactivation of the aromatase, including a portion of the ketoreductase, which was disrupted by inserting the thiostrepton gene.
Keywords
Aculose; Amicetose; Angucycline; Polyketide; Sch 47554; Sch 47555; Streptomyces;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 11  (Related Records In Web of Science)
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