• 대한의생명과학회지
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• 제29권4호
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• pp.242-248
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• 2023

Platelets are activated at the sites of vascular injury by a number of molecules, including adenosine diphosphate, collagen and thrombin. The full platelet aggregation is absolutely essential for the normal hemostasis. Glycyrrhiza glabra is a well-known medicinal herb that grows in various parts of the world and is known to have various effects such as antioxidant, anti-inflammatory, anti-atherogenic, anti-osteoporotic and skin-whitening. However, the platelet inhibitory effect of Glycyrrhiza glabra extract (GGE) has not been identified. In this study, we investigated if GGE inhibited platelet aggregation. We observed that GGE inhibited collagen-induced platelet aggregation, Ca²⁺ mobilization, and thromboxane A2 generation. In addition, GGE suppressed phosphorylation of phosphatidylinositol-3 kinase (PI3K), Akt and elevated phosphorylation of inositol 1,4,5-trisphosphate receptor (IP3R), vasodilator stimulated phosphoprotein (VASP). Taken together, GGE showed strong antiplatelet effects and may be used to block platelet-mediated cardiovascular diseases.

• 대한임상검사과학회지
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• 제49권4호
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• pp.477-488
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• 2017

이 연구는 임상병리검사학(또는 임상검사과학, 의학검사과학, 의생명검사과학)에 대한 체계적 인 접근을 하기 위해 임상병리검사학의 정체성과 학문분류체계를 가지고 논의하였다. 임상병리검사학은 한국 연구재단의 학술연구분야분류에 등재되어 있지 않다. 국내에서는 1963년 임상병리검사학과 최초로 신설된 이후 전국에 임상병리검사학과가 52개에 이르고 있다. 학문적 정체성에도 불구하고 제도적으로 임상병리검사학은 전문적 영역을 확보하지 못하고 있는 실정이다. 학술연구분야분류를 보면 물리치료학, 작업치료학, 치위생학은 체계적으로 분류되어 그 학문성을 인정받고 있다. 이 연구는 임상병리검사학의 새로운 학문분류체계이다. 내용 연구는 다음과 같이 요약된다. 임상병리사의 학문은 대분류 의약학, 중분류 임상병리학, 소분류 임상병리검사학에 위치한다. 세분류의 학문용어는 "혈액수혈학, 면역생화학, 미생물기생충학, 유전분자생물학, 조직세포학, 심폐신경생리학"으로 구성한다.

• 대한의생명과학회지
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• 제22권4호
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• pp.127-139
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• 2016

A species of the fungal genus Cordyceps has been used as an ingredient of traditional Chinese medicine. In this study, we prepared cordycepin-enriched WIB-801CE, an ethanol extract from culture solution of Cordyceps militaris-hypha, and evaluated its antiplatelet effects on human platelet aggregation. WIB-801CE dose-dependently inhibited ADP-, collagen-, and thrombin-induced platelet aggregation. These antiplatelet effects by WIB-801CE were associated with the attenuation of thromboxane $A_2$ ($TXA_2$) production and serotonin release by ADP, collagen, and thrombin. The inhibition of $TXA_2$ production by WIB-801CE was due to the inhibition of cyclooxygenase-1, $TXA_2$ synthase, and cytosolic phospholipase $A_2$ activity. Therefore, these data suggest that WIB-801CE may be a beneficial component against protection from platelet aggregation-mediated thrombotic disease.

• 대한의생명과학회지
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• 제10권1호
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• pp.9-13
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• 2004

We have investigated the effects of hypha-water extracts (HWE), fruit body-water extracts (FWE) and cordycepin from Cordyceps militaris on serotonin release out of human platelets and human platelet aggregation. HWE and FWE inhibited the release of [$^3H$]-serotonin from human platelet stimulated by thrombin (2 U/ml) or collagen (20$\mu$g/ml) in a dose-dependent manner. Furthermore, cordycepin, a major component of Cordyceps militaris, inhibited the human platelet aggregation induced by collagen (10$\mu$g/ml) in a dose-dependent manner. These results suggest that cordycepin containing in HWE and FWE may inhibit the serotonin release by suppressing the collagen-induced human platelet aggregation. Accordingly, our data demonstrate that HWE and FWE containing much cordycepin might have antithrombotic and antimigrainous functions.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 2007년도 춘계 학술대회
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• pp.21-21
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• 2007

• 대한의생명과학회지
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• 제10권3호
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• pp.195-202
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• 2004

Lung cancer is a leading cause of cancer death worldwide; however, despite major advances in cancer treatment during the past two decades, the prognostic outcome of lung cancer patients has improved only minimally. This is largely due to the inadequacy of the traditional screening approach of diagnosis in lung cancer, which detects only well­established overt cancers and fails to identify precursor lesions in premalignant conditions of the bronchial tree. In recent years this situation has fundamentally changed with the identification of molecular abnormalities characteristic of premalignant changes; these concern tumour suppressor genes, loss of heterozygosity at crucial sites and activation of oncogenes. Basic knowledge at the molecular level has extremely important clinical implications with regard to early diagnosis, risk assessment and prevention, and therapeutic targets. In this study we used a 'cap-finder' subtractive hybridization method, 'long distance' polymerase chain reaction (PCR), streptavidin magnetic beads mediated subtraction, and spin column chromatography to detect differential expression genes of human small cell lung carcinoma. We have now isolated ninety two genes that expressed differentially in the human small cell lung carcinoma cells and analyzed of 12 clones with sequencing, nine cDNAs include tapasin (NGS-17) mRNA, BC200 alpha scRNA, chromosome 12q24 PAC RPCI3-462E2, protein phosphatase 1 (PPPICA), translocation protein 1 (TLOC1), ribosomal protein S24 (RPS24) mRNA, protein phosphatase (PPEF2), cathepsin Z, MDM2 gene and three novel genes. They may be oncogenesis­related proteins.

• 대한임상검사과학회지
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• 제42권2호
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• pp.103-109
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• 2010

The aim of this study was to investigate the problem areas in the current clinical training of biomedical laboratory science education which may guide the development of an effective strategy for managing clinical training for biomedical laboratory science students. A self-reporting questionnaire was developed by the researchers. This survey was conducted between May 1st and May 15st in 2009 and 92 questionnaires were collected from senior biomedical laboratory science students. In Analyzing data, chi-square test was used for categorical variables. 82.6% of respondents was satisfied with conditions of the training institutes. 30~40% of the respondents reported that they were not satisfied with the programs and supervision systems for training students even though the staff members of other disciplines were supportive in general. Although 83.7% of the respondents reported that they were obtaining information and knowledge for their professional carrier through the training, 70.7% of the respondents reported that they had negative experiences during the training. According to the result of the survey, it can be inferred that the structure of the training education needs to be more systematically organized in order to train the well prepared entry-level biomedical laboratory scientists.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 2006년도 춘계학술대회
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• pp.77-77
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• 2006

• 대한의생명과학회지
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• 제12권4호
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• pp.303-310
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• 2006

Ankyrins are a ubiquitously expressed family of intracellular adaptor proteins involved in targeting diverse proteins to specialized membrane domains in both the plasma membrane and the endoplasmic reticulum. We described here that the C-terminal domain of ankyrin-B interact specifically with Z-line portion of titin in yeast two-hybrid analysis, in vitro pull-down assays and localization experiments in COS-7 cells. In this study we provide the first experimental evidence that Z-line portion of titin is necessary for the localization of ankyrin-B and ankyrin-B links between the sarcolemma and the myofibril in costameres.

• 대한의생명과학회지
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• 제15권4호
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• pp.265-272
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• 2009

Recently, mesenchymal stem cells (MSCs) began to be utilized as a vehicle for ex vivo gene therapy based on their plasticity. Effective and safe transfection of therapeutic genes is a critical step for genetic modification of MSCs. Therefore, optimization of in vitro gene delivery into MSCs is essential to provide genetically modified stem cells. In this study, various cationic liposomes, O,O'-dimyristyl-N-lysyl aspartate (DMKD), DMKD/cholesterol, O,O'-dimyristyl-N-lysyl glutamate (DMKE), DMKE/cholesterol, and N-[1-(2,3-dioleoyloxy)]-N,N,N-trimethylammonium propane methyl sulfate (DOTAP)/cholesterol, were mixed with plasmid DNA encoding luciferase (pAAV-CMV-Luc) at varied ratios, and then used for transfection to MSCs under varied conditions. The MSCs were also transfected by electroporation under varied conditions, such as voltage, pulse length, and pulse interval. According to the experimental results, electroporation-mediated transfection was more efficient than cationic liposome-mediated transfection. The best MSC transfection was induced by electroporation 3 times pulses for 2 ms at 200 V with 10 seconds of a pulse interval.