• Title/Summary/Keyword: Biomaterial science

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Prediction and Annotation of ABC Transporter Genes from Magnaporthe oryzae Genome Sequence (벼도열병균 게놈서열로부터 ABC transporter 유전자군의 예측 및 특성 분석)

  • Kim, Yong-Nam;Kim, Jin-Soo;Kim, Su-Young;Kim, Jeong-Hwan;Lee, Jong-Hwan;Choi, Woo-Bong
    • Journal of Life Science
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    • v.20 no.2
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    • pp.176-182
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    • 2010

  • Magnaporthe oryzae is destructive plant-pathogenic fungus and causes rice blast. The pathogen uses several mechanisms to circumvent the inhibitory actions of fungicides. ATP-binding cassette (ABC) transporters are known to provide protection against toxic compounds in the environment. PC facilitated bioinformatic analysis, particularly with respect to accessing and extracting database information and domain identification. We predicted ABC transporter genes from the M. oryzae genome sequence with computation and bioinformatics tools. A total of thirty three genes were predicted to encode ABC transporters. Three of thirty three putative genes corresponded to three known ABC transporter genes (ABC1, ABC2 and ABC3). Copy numbers of the ABC transporter genes were proven by Southern blot analysis, which revealed that twenty genes tested exist as a single copy. We amplified the DNA complementary to RNA corresponding to eleven of these by reverse transcriptase polymerase chain reaction.

  • https://doi.org/10.5352/JLS.2010.20.2.176 인용 PDF KSCI

Enhancement of Low Molecular Ginsenoside Contents in Low Quality Fresh Ginseng by Fermentation Process (등외품 인삼(파삼)의 유산균 발효에 의한 저분자 진세노사이드 함량 증진)

  • Choi, Woon-Yong;Lee, Choon-Geun;Song, Chi-Ho;Seo, Yong-Chang;Kim, Ji-Seon;Kim, Bo-Hyeon;Shin, Dae-Hyun;Yoon, Chang-Soon;Lim, Hye-Won;Lee, Hyeon-Yong
    • Korean Journal of Medicinal Crop Science
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    • v.20 no.2
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    • pp.117-123
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    • 2012

  • This study compared the contents of low molecular ginsenoside according to fermentation process in low grade fresh ginseng. Low grade fresh ginseng was directly inoculated with a 24 h seed culture of $Bifidobacterium$ Longum B6., $Lactobacillus$ $casei$., and incubated at $36^{\circ}C$ for 72 h. $Bifidobacterium$ Longum B6 was specifically was found to show the best growth on $3,255{\times}10^6\;CFU/m{\ell}$ after 48 h of fermentation. The content of ginsenoside Rb1, Re and Rd were decreased with the fermentation but ginsenoside Rh2 and Rg2 increased after fermentation process. In the case of low molecular ginsenoside conversion yields were 56.07% of Rh2, 12.03% of Rg3 and 77.11% of Rg2, respectively. In addition, compound-K was irregular conversion yield as long as 72 h of fermentation. This results indicate that fermentation process could increase the low molecular ginsenoside in low grade fresh ginseng.

  • https://doi.org/10.7783/KJMCS.2012.20.2.117 인용 PDF KSCI