• 한국당과학회:학술대회논문집
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• 한국당과학회 2006년도 제1회 연례학술대회
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• pp.53-54
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• 2006

• 한국고분자학회지:고분자과학과기술
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• 제18권5호
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• pp.450-457
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• 2007

• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1213-1216
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• 2007

The vector pCW5 with plasmid pC7, originally isolated in Lactobacillus paraplantarum C7 derived from kimchi, was constructed using a p32 strong promoter, the pC7 replicon, and green fluorescent protein (GFP) as the reporter. The constructed vector was transformed into E. coli and Leuconostoc mesenteroides, and GFP expression detected using a Western blot analysis. GFP fluorescence was recognized in E. coli and Leuconostoc mesenteroides using a confocal microscope. In addition, GFP fluorescence was also clearly detected in several industrially important lactic acid bacteria (LAB), including Lactobacillus bulgaricus, Lactobacillus paraplantarum, and Lactobacillus plantarum. Thus, pCW5 was shown to be effective for Leuconostoc mesenteroides when using GFP as the reporter, and it can also be used as a broad-host-range vector for other lactic acid bacteria.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.392-392
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• 2005

• 한국의류산업학회지
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• 제14권4호
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• pp.677-683
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• 2012

1-octanethiol and vinyl acetate telomers ($R_8S$-nVAc) were synthesized and hydrolyzed with sodium hydroxide subsequently, 1.2-epoxyhexane was then introduced to the telomers. In addition, we prepared cotelomers of multi-alkylated nonionic surfactants with a molecular structure of xRnMA-yVA (x; hydrophobic group, y; hydrophilic group, MA; methacrylic ester, VA; vinyl alcohol, R; and alkyl group) and cross-linked with sodium tetraborate decahydrate. Their active surface properties were investigated by several techniques such as surface tension, foaming property, and emulsification power measurements. The surface tension of $R_8S$-8.8VA decreased without the introduction of 1.2-Epoxy hexane, and the degree of emulsification and foaming abilities of $R_8S$-8.8VA increased without the introduction of 1.2-Epoxy hexane. However, the differences were insignificant. The epoxy groups were attached to a $R_8S$-8.8VA cotelomer with a limited variation of the active surface properties. The surface tension of $1.1R_6MA$-8.8VA decreased after cross-linking subsequently, the degree of emulsification and foaming abilities of $1.1R_6MA$-8.8VA increased after cross-linking. However, there was no clear difference between them. The B-O bonds were attached to a $1.1R_6MA$-8.8VA cotelomer with a limited variation of the active surface properties.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2006년도 제47회 학술심포지움 및 추계국제학술대회
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• pp.38.2-38.2
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• 2006

• 한국고분자학회지:고분자과학과기술
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• 제16권4호
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• pp.468-477
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• 2005

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2008년도 제49회 학술심포지움 및 국제학술대회
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• pp.49.1-49.1
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• 2008

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2007년도 제48회 학술심포지움 및 추계국제학술대회
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• pp.56.2-56.2
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• 2007

See Full Text

• Journal of Microbiology and Biotechnology
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• 제22권6호
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• pp.806-813
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• 2012

Adipocyte differentiation is strongly associated with obesity, which causes metabolic disorders. In this study, we investigated the inhibitory effects of widdrol on 3T3-L1 preadipocyte growth and differentiation. Widdrol decreased lipid droplet accumulation and down-regulated adipogenic transcription factors such as C/$EBP{\alpha}$, C/$EBP{\beta}$, and $PPAR{\gamma}$. Widdrol blocked preadipocyte proliferation and differentiation through the inhibition of mitotic clonal expansion, which was accompanied by the failure of degradation of p21, a cyclin-dependent kinase inhibitor. Cell-cycle analysis clearly indicated that widdrol actively induces cell-cycle arrest at the G1-S phage transition, causing cells to remain in the preadipocyte state. Moreover, widdrol increased p21 expression and inhibited Rb phosphorylation in preadipocyte incubated in a hormone medium. Therefore, these findings clearly suggest that widdrol blocks preadipocyte growth and differentiation through the inhibition of mitotic clonal expansion by p21-and Rb-dependent G1 arrest and can be developed as a potent anti-adipogenic agent for reducing obesity.