• Environmental Engineering Research
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• 제24권1호
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• pp.127-136
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• 2019

Land application of treated palm oil mill effluent (TPOME) could be used as an alternative tertiary wastewater treatment process. However, phenolic compounds in TPOME might be leached to the environment. This study investigated the ability of grasses on reducing phenolic compounds in the leachate after TPOME application. Several pasture grasses in soil pots were compared after irrigating with TPOME from stabilization ponds, which contained 360-630 mg/L phenolic compounds. The number of soil bacteria in planted pots increased over time with the average of $10^8CFU/g$ for mature grasses, while only $10^4-10^6CFU/g$ were found in the unplanted control pots. The leachates from TPOME irrigated grass pots contained lower amounts of phenolic compounds and had lower phytotoxicity than that of control pots. The phenol removal efficiency of grass pots was ranged 67-93% and depended on grass cultivars, initial concentration of phenolic compounds and frequency of irrigations. When compared to water irrigation, TPOME led to an increased phenolic compounds accumulation in grass tissues and decreased biomass of Brachiaria hybrid and Brachiaria humidicola but not Panicum maximum. Consequently, the application of TPOME could be conducted on grassland and the grass species should be selected based on the utilization of grass biomass afterward.

• 한국농림기상학회:학술대회논문집
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• 한국농림기상학회 2015년도 하계 학술발표논문집
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• pp.18-28
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• 2015

Apple is cultivated under various climatic conditions in many parts of the world. Better understanding of how climate, genotype, soil, and management factors interact to determine crop productivity will improve our ability to optimize crop selection, management strategies, and resource use efficiencies. We developed and applied a physiology-based apple canopy model to evaluate how climatic factors and crop phenotypes interact to determine biomass accumulation, radiation use efficiency (RUE), and water use efficiency (WUE) at multiple production sites between western and eastern states of the US including WA, CA, NY, WV, and PA. Our results indicate that solar radiation is a dominant factor limiting biomass production in the eastern states while VPD is the primary factor governing crop water use across eastern and western states during the peak growing season. Crop RUE and WUE were strongly correlated in the western states but not in the eastern states while VPD showed highly negative correlation with both RUE and WUE across all locations. The RUE improved with increasing fraction of diffuse radiation ($f_{df}$) and the $RUE-f_{df}$ relationships revealed distinctive responses between western and eastern states. Overall, the eastern locations exhibited slightly higher RUE and WUE than the western locations. However, overall productivity and total water use were greater in the western states. A clear decline of productivity with increasing temperature and afternoon VPD past an optimum was predicted in the western locations but this pattern was less clear in the eastern locations. We also discuss potential phenotypes with specific physiological and morphological traits that are differentially suitable for western and eastern locations. Our results provide plausible, spatially explicit explanations and insights to disentangle the complex relationships between crop productivity, resource use efficiencies, phenotype, and climate drivers in apple grown in the US.

• 한국산림과학회지
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• 제82권2호
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• pp.188-194
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• 1993

임령(林齡) 및 환경조건(環境條件)이 유사한 굴참나무천연림(天然林)과 현사시나무인공림(人工林)의 물질생산(物質生産)을 조사(調査) 비교(比較)하기 위하여 전라남도(全羅南道) 모후산지역(母后山地域) 순천대학교(順天大學校) 연습림(演習林)에 위치하고 있는 평균(平均) 20년생(年生) 굴참나무천연림(天然林)과 17년생(年生) 현사시나무인공림(人工林)을 대상으로 각각 $20m{\times}30m$ 조사구를 설치하고 10주씩의 표본목을 선정한 후 뿌리를 제외한 지상부(地上部)의 현존량(現存量), 순생산량(純生産量) 등을 조사하였다. 수종별(樹種別), 부위별(部位別) 현존량(現存量) 추정식(推定式)을 유도 검정한 결과 흉고직경(胸高直徑)(D)과 수고(樹高)(H)를 독립변수(獨立變數)로 하는 상대성장식(相對成長式)(logWt=A+BlogD+ClogH)이 흉고직경(胸高直徑)만을 독립변수(獨立變數)로 하는 상대성장식(相對成長式)(logWt=A+BlogD)에 비하여 적합도(適合度)가 다소 높은 경향을 보였으나 큰 차이는 없는 것으로 나타났다. 임분(林分) 전체(全體)의 지상부(地上部) 현존량(現存量)은 현사시나무림이 55,581kg/ha로서 굴참나무림의 31,275kg/ha보다 1.8배 정도 많았다. 부위별(部位別) 현존량(現存量) 구성비(構成比)는 굴참나무림과 현사시나무림 모두 줄기의 목질부(木質部), 가지, 수피(樹皮), 잎의 순(順)으로 높았으나, 굴참나무림의 경우 현사시나무림에 비하여 수피(樹皮), 가지, 잎의 구성비(構成比)는 높은 반면 줄기의 목질부(木質部) 구성비(構成比)는 낮았다. 임분(林分) 전체의 지상부(地上部) 순생산량(純生産量)은 굴참나무림이 4,267kg/ha/yr.이었으며 현사시나무림은 3,903kg/ha/yr.이었다. 부위별(部位別) 순생산량(純生産量) 구성비(構成比)는 굴참나무림의 경우 잎, 줄기의 목질부(木質部), 가지, 수피(樹皮)의 순으로 높았으며 현사시나무림은 줄기의 목질부(木質部), 잎, 가지, 수피(樹皮)의 순이었다. 순동화율(純同化率)은 현사시나무림이 3.376으로서 굴참나무림의 2.121보다 1.6배 정도 높았으며, 잎의 줄기 생산능률(生産能率)의 경우 현사시나무림이 굴참나무림에 비하여 2.4배 정도 높았다. 현사시나무림은 굴참나무림에 비하여 순동화율(純同化率)이 높은 반면 잎의 현존량(現存量)이 적기 때문에 지상부(地上部) 전체 순생산량(純生産量)은 적었으나, 지속적 축적기관(蓄積器官)인 줄기의 목질부 순생산량(純生産量)이 많기 때문에 임분(林分) 현존량(現存量)이 많은 것으로 나타났다.

• Journal of Crop Science and Biotechnology
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• 제11권2호
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• pp.111-118
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• 2008

Soybean is sensitive to waterlogging stress, leading to reduce their growth and yield significantly. The objective of this study was to characterize the relative sensitivities of biomass accumulation and specific nodule activity under waterlogging stress between supernoduating mutants, 'SS2-2' and 'Sakukei 4' and their wild-type soybeans, 'Sinpaldalkong 2' and 'Enrei', respectively. Flooding treatment was performed to soybean plants grown in a pot by waterlogging for 15 days from the beginning bloom(R1) stage under natural light. The nodule number and weight were considerably decreased by waterlogging stress. The bleeding sap rate of waterlogging soybean plants was decreased by 78-80% in supernodulating mutants and 65-74% in their wild types compared to control plants. The relative ureide-N content was also decreased by waterlogging and the reduction was high in supernodulating mutants. This may cause the massive reduction of shoot and root dry weight and leaf area in waterlogged soybean plants. There was a varietal difference in response to the waterlogging stress. During the waterlogging, supernodulating mutants maintained higher spad value than their wild types. Particularly, the difference between soybean varieties was clear in low rank leaves from the top. Also, supernodulating mutants showed a weak waterlogging tolerance than their wild types. Under waterlogging conditions, massive nodules were considerably destroyed and specific nodule activity after waterlogging may not be recovered when compared to their wild-type soybeans. Supernodulating mutants showed lower seed yield than their wild types in waterlogging conditions.

• Plant Biotechnology Reports
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• 제4권2호
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• pp.109-116
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• 2010

Morinda citrifolia adventitious roots were cultured in shake flasks using Murashige and Skoog medium with different types and concentrations of auxin and cytokinin. Root (fresh weight and dry weight) accumulation was enhanced at 5 $mg\;l^{-1}$ indole butyric acid (IBA) and at 7 and 9 $mg\;l^{-1}$ naphthalene acetic acid (NAA). On the other hand, 9 $mg\;l^{-1}$ NAA decreased the anthraquinone, phenolic and flavonoid contents more severely than 9 $mg\;l^{-1}$ IBA. When adventitious roots were treated with kinetin (0.1, 0.3 and 0.5 $mg\;l^{-1}$) and thidiazuron (TDZ; 0.1, 0.3 and 0.5 $mg\;l^{-1}$) in combination with 5 $mg\;l^{-1}$ IBA, fresh weight and dry weight decreased but secondary metabolite content increased. The secondary metabolite content (including 1,1-diphenyl-2-picrylhydrazyl activity) increased more in TDZ-treated than in kinetin-treated roots. Antioxidative enzymes such as catalase (CAT) and guaiacol peroxidase (G-POD), which play important roles in plant defense, also increased. A strong decrease in ascorbate peroxidase activity resulted in a high accumulation of hydrogen peroxide. This indicates that adventitious roots can grow under stress conditions with induced CAT and G-POD activities and higher accumulations of secondary metabolites. These results suggest that 5 $mg\;l^{-1}$ IBA supplementation is useful for growth and secondary metabolite production in adventitious roots of M. citrifolia.

• 한국약용작물학회지
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• 제14권6호
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• pp.371-375
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• 2006

To investigate effects on the growth and ginsenosides accumulation in ginseng hairy root, potassium phosphate was supplemented with 1.25, 2.5, and 5.0 mM concentration in 1/2 MS medium, respectively. Potassium phosphate supplement was increased the biomass and ginsenosides accumulation when it was dose at the concentration of 1.25 mM. And the growth rate of hairy root in the light condition was higher than in dark condition. The highest contents and productivity of ginsenosides were observed at the supplement of 1.25 mM potassium phosphate at the 7th day after the culture onset. Ginseng hairy root cultured in 20 L bioreactor supplemented with 1.25 mM KH$_2$PO$_4$ was increased the growth with 1,609 g (F${\cdot}$W) and ginsenosides content with 11.09 mg than those in control.

• 한국약용작물학회지
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• 제12권6호
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• pp.478-482
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• 2004

공기주입형 생물반응기를 이용하여 오미자 현탁배양세포로부터 Gomisin J의 생산 가능성을 확인 하였다. 시료의 초기접종 농도와 탄소원의 농도에 따른 세포증식과 Gomisin J의 생산성은 6% sucrose가 첨가된 MB5배지 에 100 g (FCW)의 현탁배양세포를 접종하였을 경우 세포중량 (dry biomass)과 Gomisin J의 함량은 각각 43.5 g DCW/과 $0.71\;{\times}\;10^{-3}\;{\mu}g/g$ DCW을 얻을 수 있었다. 배양기내 산소의 공급량은 0.5로 공급해 주는게 바람직 할 것으로 사료되었다.

• Journal of Microbiology and Biotechnology
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• 제23권9호
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• pp.1279-1286
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• 2013

Particle size and metal species are important to both soil microbial toxicity and phytotoxicity in the soil ecosystem. The effects of CuO and ZnO nanoparticles (NPs) and microparticles (MPs) on soil microbial toxicity, phytotoxicity, and bioaccumulation in two crops (Cucumis sativus and Zea mays) were estimated in a soil microcosm. In the microcosm system, soil was artificially contaminated with 1,000 mg/kg CuO and ZnO NPs and MPs. After 15 days, we compared the microbial toxicity and phytotoxicity by particle size. In addition, C. sativus and Z. mays were cultivated in soils treated with CuO NPs and ZnO NPs, after which the treatment effects on bioaccumulation were evaluated. NPs were more toxic than MPs to microbes and plants in the soil ecosystem. We found that the soil enzyme activity and plant biomass were inhibited to the greatest extent by CuO NPs. However, in a Biolog test, substrate utilization patterns were more dependent upon metal type than particle size. Another finding indicated that the metal NP uptake amounts of plants depend on the plant species. In the comparison between C. sativus and Z. mays, the accumulation of Cu and Zn by C. sativus was noticeably higher. These findings show that metal oxide NPs may negatively impact soil bacteria and plants. In addition, the accumulation patterns of NPs depend on the plant species.

• Environmental Engineering Research
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• 제14권4호
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• pp.226-236
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• 2009

The performance of phytoremediation has proven effective in the removal of nutrients and metals from aqueous systems. However, little information is available regarding the behavior of pesticides and their removal pathways in aquatic environments involving plant-uptake. A detailed understanding of the kinetics of pesticide removal by plants and information on compound/plant partition coefficients can lead to an effective design of the phytoremediation process for anthropogenic pesticide reduction. It was determined that the reduction rates of four organophosphorus (OP) and two organochlorine (OC) pesticides (diazinon, fenitrothion, malathion, parathion, dieldrin, hexachlorobenzene [HCB]) could be simulated by first-order reaction kinetics. The magnitude of k was dependent on the pesticide species and found within the range of 0.409 - 0.580 $d^{-1}$. Analytical results obtained by mass balances suggested that differential chemical stability, including diversity of molecular structure, half-lives, and water solubility, would greatly influence the removal mechanisms and pathways of OPs and OCs in a phytoreactor (PR). In the case of OP pesticides, plant accumulation was an important pathway for the removal of fenitrothion and parathion from water, while pesticide sorption in suspended matter (SM) was an important pathway for removal of dieldrin and HCB. The magnitude of the pesticide migration factor (${\Large M}_p^{pesticide}$) is a good indication of determining the tendency of pesticide movement from below- to above-ground biomass. The uncertainties related to the different phenomena involved in the laboratory phyto-experiment are also discussed.

• 한국환경과학회지
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• 제16권12호
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• pp.1345-1353
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• 2007

The effect of salicylic acid(SA) on antioxidant system and protective mechanisms against UV-B induced oxidative stress was investigated in cucumber(Cucumis sativus L.) leaves. UV-B radiation and SA were applied separately or in combination to first leaves of cucumber seedlings, and dry matter accumulation, lipid peroxidation and activities of antioxidant enzymes were measured in both dose and time-dependant manner. UV-B exposure showed reduced levels of fresh weight and dry matter production, whereas SA treatment significantly increased them. SA noticeably recovered the UV-B induced inhibition of biomass production. UV-B stress also affected lipid peroxidation and antioxidant enzyme defense system. Malondialdehyde(MDA), a product of lipid peroxidation, was greatly increased under UV-B stress, showing a significant enhancement of a secondary metabolites, which may have antioxidative properties in cucumber leaves exposed to UV-B radiation. Combined application of UV-B and SA caused a moderate increase in lipid peroxidation. These results suggest that SA may mediate protection against oxidative stress. UV-B exposure significantly increased SOD, APX, and GR activity compared with untreated control plants. Those plants treated with 1.0 mM SA showed a similar pattern of changes in activities of antioxidant enzymes. SA-mediated induction of antioxidant enzyme activity may involve a protective accumulation of $H_2O_2$ against UV-B stress. Moreover, their activities were stimulated with a greater increase by UV-B+SA treatment. The UV-B+SA plants always presented higher values than UV-B and SA plants, considering the adverse effects of UV-B on the antioxidant cell system. ABA and JA, second messengers in signaling in response to stresses, showed similar mode of action in UV-B stress, supporting that they may be important in acquired stress tolerance. Based on these results, it can be suggested that SA may participates in the induction of protective mechanisms involved in tolerance to UV-B induced oxidative stress.