• 웰빙융합연구
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• 제5권4호
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• pp.51-56
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• 2022

Purpose: By applying an ultrasonic mechanical device to the liquid fertilizer storage in the pig dropping treatment plant, the initial odor of the odor source is reduced, and the air dilution drainage of the complex odor is fundamentally recognized to facilitate odor treatment on the mechanical and chemical biological treatment devices at the rear. Research design, data and methodology: The odor concentration on the site boundary was measured to confirm the state of reduction. In order to prevent the spread of odor from the collection of the pig dropping treatment plant, it was measured by installing an ultrasonic generator inside the installation wall after installing the sealing wall. Results: The average value of the March and April measurement data remained close to neutral at 8.2 after 8.6 treatment before pH treatment, decreased 97.3% from 462 mg/L before SS treatment to 10.5 mg/L after treatment, and the composite odor was reduced by 85% from 20 to 3 before treatment. It was confirmed that ammonia (NH3) was reduced by 99% from 5.8 ppm to 0.09 ppm, and general bacteria were also reduced by 99% from 3,200 CFU/mL to 57 CFU/mL Conclusion: Applying the ultrasonic air ejector hybrid system and zigzag air complex module development product to resource circulation centers or sewage treatment facilities is thought to reduce inconvenience to residents due to odors caused.

• Journal of Microbiology and Biotechnology
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• 제32권4호
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• pp.531-540
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• 2022

Due to the high incidence of malignant melanoma, the establishment of in vitro models that recapitulate the tumor microenvironment is of great biological and clinical importance for tumor treatment and drug research. In this study, 3D printing technology was used to prepare GelMA/PEGDA composite scaffolds that mimic the microenvironment of human malignant melanoma cell (A375) growth and construct in vitro melanoma micro-models. The GelMA/PEGDA hybrid scaffold was tested by the mechanical property, cell live/dead assay, cell proliferation assay, cytoskeleton staining and drug loading assay. The growth of tumor cells in two- and three-dimensional culture systems and the anti-cancer effect of luteolin were evaluated using the live/dead staining method and the Cell Counting Kit-8 (CCK-8) method. The results showed a high aggregation of tumor cells on the 3D scaffold, which was suitable for long-term culture. Cytoskeleton staining and immunofluorescent protein staining were used to evaluate the degree of differentiation of tumor cells under 2D and 3D culture systems. The results indicated that 3D bioprinted scaffolds were more suitable for tumor cell expansion and differentiation, and the tumor cells were more aggressive. In addition, luteolin was time- and dose-dependent on tumor cells, and tumor cells in the 3D culture system were more resistant to the drug.