• Journal of the Ergonomics Society of Korea
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• v.27 no.2
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• pp.83-89
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• 2008

Study was developed the metallic plate for fixation in the femur fracture and plates has a firm place in fracture treatment. This plates can be stabilized for fracture fixation as well as biological and dynamical device. The device's designation and sizing has a optimization with bending structural stiffness and strength, known meaning that is reliable regardless of the plate by the short type and long type. The bending strength of the curved metallic long plate has to evaluate a 11,000N and the bending strength of the curved metallic short plate has to evaluate a 6,525N. This see the X-ray image of bending angle made certain of 15$^{\circ}$ at number 2 and same 82.87$^{\circ}$ at number 2, 4, 5, 7, 8, 9, 10 by outside angle, and confirmed 25.26$^{\circ}$ at number 3, 3.68$^{\circ}$ at number 6, 15.64$^{\circ}$ at number 9 by inside angle. This study shows that keep up the metallic plate for fixation in the femur fracture through X-ray Image and the device can be used to support Revision case of Hip Implant and to use a case of Hip screw compression of Hip Neck Fracture. Short plate have a wrapping of femur and long plate have to preserve a pole of femur.

• The Journal of the Korean dental association
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• v.55 no.10
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• pp.676-687
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• 2017

Objectives : The aim of this study was to evaluate the effects of a composite of bone substitute and collagen barrier membrane (bone patch) for local ridge augmentation at peri-implant dehiscence defects on the clinical efficacy and positional stability in dogs. Materials and methods : Implant placement and ridge augmentation procedure were performed at surgically created peri-implant dehiscence defects in canine mandible (n=6). Four treatment modalities were randomly applied: i) bone patch group, ii) Guided bone regeneration (GBR) without pin fixation group (bone graft and collagen membrane), iii) GBR with pin fixation group, and iv) negative control group. After 12 weeks, clinical, micro-CT and histological analyses were performed. Results : Histologic analysis showed that bone patch group had similar results to GBR group and GBR with fixation group in terms of new bone formation. Micro-CT analysis revealed similar results to histologic analysis in terms of total volume maintenance. Operating time was shorter in bone patch group compared to GBR group and GBR with fixation groups. Conclusions : GBR using bone patch could simplify the ridge augmentation procedure with reduced operating time and equivalent biological performance compared to the conventional procedure.

• Applied Chemistry for Engineering
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• v.21 no.3
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• pp.243-251
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• 2010

Nitrogen oxides (NOx) in combustion flue gas are currently mitigated by chemical processes such as catalytic reduction, absorption and adsorption. However, development of environmentally sustainable biological processes is necessary in the near future. In this paper, the up-to-dated R&D trend of biological methodologies regarding NOx removal was reviewed, and their advantages and disadvantages were discussed. The principles and applications of bacterial system including nitrification and denitrification and photosynthetic microalgae system were compared. In order to enhance biological treatment rate and performance, the insoluble nitric oxide (NO) should be first absorbed using a proper solubilization agent, and then microbial degradation or fixation is to be followed. The use of microalgal system has a good prospect because it can fix $CO_2$ and NOx simultaneously and requires no additional carbon for energy source.

• The Korean Journal of Zoology
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• v.38 no.3
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• pp.417-425
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• 1995

The present study was carried out to investigate the effect of microwave fixation in comparison with that of chemical fixation in preparing the microscopic samples. The microwave fixator was equipped with infrared-temperature sensor, and that was designed to compensate air temperature in the microwave fixator. In the microwave fixation, rat tongue was well preserved in terms of muscular fasciculus and pancreas stained by Feulgen reagents showed clear reaction products in the nucleus. Reaction products by PAS method in duodenal villi appeared specifically at the goblet cells. In electron microscopy, pancreatic cellular components such as secretory granules and collagen bundles were well preserved in both fixations. In aspect of histochemical reaction and electron microscopy, high quality was due to the protein content of microwave fixed specimen. The microwave fixation method saved total duration engaging microscopic preparation.

• Applied Microscopy
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• v.51
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• pp.16.1-16.7
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• 2021

Electron microscopy (EM) is an essential imaging method in biological sciences. Since biological specimens are exposed to radiation and vacuum conditions during EM observations, they die due to chemical bond breakage and desiccation. However, some organisms belonging to the taxa of bacteria, fungi, plants, and animals (including beetles, ticks, and tardigrades) have been reported to survive hostile scanning EM (SEM) conditions since the onset of EM. The surviving organisms were observed (i) without chemical fixation, (ii) after mounting to a precooled cold stage, (iii) using cryo-SEM, or (iv) after coating with a thin polymer layer, respectively. Combined use of these techniques may provide a better condition for preservation and live imaging of multicellular organisms for a long time beyond live-cell EM.

• Journal of Applied Biological Chemistry
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• v.56 no.4
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• pp.235-239
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• 2013

This study was carried out to investigate the plant growth promoting activity of hairy vetch (Vicia villosa Roth) on reclaimed land. At the previous research, Rhizobium sp. RH84 was isolated and selected for further study from hairy vetch. For the investigation of plant growth promoting effects by the Rhizobium sp. RH84, production of indole acetic acid (IAA), siderophore, phosphate solubilization and nitrogen fixation were tested and other characters were examined. As results, RH84 produced $9.03{\mu}g$ IAA per mL and showed nitrogen fixation activity. With the treatment of Rhizobium sp. RH84 to hairy vetch showed good growth at 0.3% salty reclaimed soil, and the production yield was increased up to 56% at field test. From these results, it was confirmed that the Rhizobium sp. RH84 would be used as a green manure for hairy vetch under the salty condition of reclaimed land.

• Journal of Microbiology and Biotechnology
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• v.25 no.8
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• pp.1339-1348
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• 2015

Until now, considerable effort has been made to engineer novel nitrogen-fixing organisms through the transfer of nif genes from various diazotrophs to non-nitrogen fixers; however, regulatory coupling of the heterologous nif genes with the regulatory system of the new host is still not well understood. In this work, a 49 kb nitrogen fixation island from P. stutzeri A1501 was transferred into E. coli using a novel and efficient transformation strategy, and a series of recombinant nitrogen-fixing E. coli strains were obtained. We found that the nitrogenase activity of the recombinant E. coli strain EN-01, similar to the parent strain P. stutzeri A1501, was dependent on external ammonia concentration, oxygen tension, and temperature. We further found that there existed a regulatory coupling between the E. coli general nitrogen regulatory system and the heterologous P. stutzeri nif island in the recombinant E. coli strain. We also provided evidence that the E. coli general nitrogen regulator GlnG protein was involved in the activation of the nif-specific regulator NifA via a direct interaction with the NifA promoter. To the best of our knowledge, this work plays a groundbreaking role in increasing understanding of the regulatory coupling of the heterologous nitrogen fixation system with the regulatory system of the recipient host. Furthermore, it will shed light on the structure and functional integrity of the nif island and will be useful for the construction of novel and more robust nitrogen-fixing organisms through biosynthetic engineering.

• Proceedings of the KOSOMBE Conference
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• v.1997 no.11
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• pp.484-488
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• 1997

Three-dimensional finite element analyses were used to compare the stress distribution and the stability of the fixation among seven different tibial components and to investigate the effect due to implant materials in total knee arthroplasty. The components included an intact tibia(Type I), Cemented Cobalt-Chromium tibial tray implanted with a PMMA cemented Co-Cr stem(Type II), Cemented Co-Cr tibial tray with a uncemented Co-Cr stem(Type III), Cemented Ultra High Molecular Weight Polyethylene (UHMWPE) tibial tray with a cemented UHMWPE stem (Type IV), Cemented UHMWPE tray with a uncemented UHMWPE stem(Type V), Cemented Co-Cr tray without a stem(Type VI), and Cemented UHMWPE tray without a stem(Type VII). Uncemented components were assumed to have complete bony in growth and a rigid state of fixation between component and bone. The interface between bone/cement/component of cemented components was also assumed to be fully bonded. Bi-condylar forces were applied. The results indicated that Uncemented stem components provided lower bone stress shielding and stress concentration. The UHMWPE tray and stem component showed better agreement with the intact tibia than the Co-Cr Alloy tray and stem components. If the implant tray can be fixed firmed without a stem, Cemented PE tray without a stem(Type VII) may be recommended to give the best characteristics in the sense of stress distribution and stability.

• Journal of the Korea Society of Computer and Information
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• v.12 no.5
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• pp.285-292
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• 2007

This study was developed the metallic plate for fixation in the femur fracture for the orthopedic region and rigid fixation with plates has a firm place in fracture treatment. Most plates can be used for rigid as well as biological and dynamical fracture fixation. The device's designation and sizing has a specific with bending structural stiffness and strength, known meaning that is reliable regardless of the plate by the short type and long type. Short plate have a wrapping of femur and long plate have to preserve a pole of femur. The bending strength of the curved metallic long plate has to evaluate a 11,000N and The bending strength of the curved metallic short plate has to evaluate a 6,525N. The tensile stress through to press a plate is $1573N/m^2\;and\;1539N/m^2$. The device can be used to support Revision case of Hip Implant and to use a case of Hip screw compression of Hip Neck Fracture.

• Korean Journal of Agricultural Science
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• v.41 no.4
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• pp.275-281
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• 2014

The ozone depletion has caused plants to be exposed to an increased penetration of solar ultraviolet-B (UV-B) radiation. Enhanced UV-B radiation may have influence on biological functions of plant in many aspects including inhibition of photosynthesis. It is evident that UV-B can potentially impair the performance of all three main component processes of photosynthesis, the photophosphorylation reactions of the thylakoid membrane, the $CO_2$-fixation reactions of the Calvin cycle and stomatal control of $CO_2$ supply. Owing to these depressed reactions, the production and allocation of carbohydrates might be markedly affected, and therefore, the growth and development of plant are distinctly reduced. In this review paper, we provide basic theory and further researches in terms of photosynthesis and carbohydrate synthesis in response to elevated UV-B radiation.