• Title/Summary/Keyword: Biological evaluation

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Facile Synthesis and Biological Evaluation of Heterocyclic Compounds Containing Diazepam

  • Berghot, M.A.
    • Archives of Pharmacal Research
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    • v.24 no.4
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    • pp.263-269
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    • 2001
  • Diazepamoxadiazoles 4,5,6,12,14 and 22 were prepared with the binary form system. Diazepamthiadiazoles 15,20 and Diazepamtriazoles 7,8,9,17,18,19 and 21 were also shapely synthesized. Some of these compounds were screened to test their antibacterial activity against E. coli and B. subtilis compounds 15 and 20 show potent activity against these bacteria.

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A Study on Classification of Forest Wetlands Types and Inventory Establishment in Korea (한국의 산림습원 유형 구분 및 인벤토리 구축)

  • Lee, Jong-Won;An, Jong-Bin;Hwang, Tae Young;Yun, Ho-Geun
    • Journal of Wetlands Research
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    • v.24 no.1
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    • pp.1-24
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    • 2022
  • This study was conducted to perform efficient conservation and management by classifying the types of wetlands distributed in forests of Korea and assigning grades according to the evaluation system from 2015 until 2019. From 2005 to 2014, 1,264 sites derived from the first national forest wetland survey and 16 additional excavated sites were classified and also evaluated 455 out of a total of 1,280 forest wetlands. Forest wetlands are divided into four types: natural type, abandoned paddy field type, man-made type, and modified type, and by reclassifying them in detail, a total of 11 detailed wetland types could be distinguished. Based on this, evaluation was performed according to various items such as plants and ecology, hydrology and hydrology, humanities and society, and the degree of disturbance was graded. As a result, the forest wetland value was sorted at 30 A- grade sites, high-value B-grade 201 sites, moderate C-grade 184 sites, and low-value D-grade 40 sites. Forest Genetic Resource Reserve (FGRR) and other effective area -based conservation measures (OECMs) were designated for 231 sites that received a high grade of A or B, and a long-term monitoring system should be established to systematically conserve forest biodiversity hotspot. It is judged that wetlands need to be managed more effectively and at the national level.

Biological Monitoring of Arsenic Concentrations According to Exposure to Arsenic-contaminated Ground Water (모 지역 소규모급수시설 비소검출에 따른 생물학적 노출 평가)

  • Seo, Jeong-Wook;Choi, Jae-Won;Oh, Yu-jin;Hong, Young-Seoub
    • Journal of Environmental Health Sciences
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    • v.46 no.5
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    • pp.513-524
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    • 2020
  • Objective: The main purpose of this study is to evaluate the environmental and biological exposure of local residents who consumed arsenic-contaminated drinking water for less than one year. Methods: As a part of water quality inspections for small-scale water supply facilities, surveys were conducted of residents of two villages that exceeded the arsenic threshold for drinking water. The environmental impact survey consisted of surveys on water quality, soil, and crops in the surveyed area. Biological monitoring was performed by measuring the separation of arsenic species in urine and total arsenic in hair. Results: In the results of biological monitoring, the concentrations of AsIII and AsV were 0.08 and 0.16 ㎍/L, respectively. MMA and DMA were 0.87 and 36.19 ㎍/L. There was no statistically significant difference between the group who drank arsenic-removed groundwater or water from the small-scale supply facility and the group who drank tap water, purified water, or commercial bottled water. Some of the water samples exceeded the arsenic threshold for drinking water. There were no samples in the soil or rice that exceeded the acceptable threshold. Conclusion: In the case of short-term exposure to arsenic-contaminated drinking water for less than one year, there were no significant problems of concern from the evaluation of biological monitoring after arsenic was removed.

Evaluation of Sexing in Boar Sperm Using Chromogenic In Situ Hybridization (Chromogenic In Situ Hybridization을 이용한 돼지 정자의 성 선별)

  • Kim, H.H.;Roh, D.E.;Jo, T.K.;Byun, J.W.;Lee, J.W.;Kim, Y.S.;Hwang, Y.J.;Kim, D.Y.
    • Journal of Embryo Transfer
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    • v.22 no.3
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    • pp.173-178
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    • 2007
  • 돼지 정자의 성 선별에는 일반적으로 유속 세포 분석기를 이용한다. 유속 세포 분석은 DNA량의 차이에 기초하여 정자를 분리하는 기술로써 X 정자와 Y 정자를 90% 정확도로 분리할 수 있다. 그러나 이러한 유속 세포분석 기술은 정자의 손상을 야기해 정자의 기능과 수정능에 영향을 미치므로, 본 연구에서는 특정한 핵산 서열을 탐지할 수 있는 Chromogenic in situ hybridization(CISH)을 그와 비교하여 평가하였다. 유속 세포 분석을 수행하기 위해 정자를 SYBR 14와 PI로 염색하였고, histogram, dotplot, density, contour를 측정하였다. Y 염색체 특이적인 primer를 이용한 PCR로 유속 세포 분석의 정확도를 검사하였다. HRP/DAB 시스템에 기초한 CISH 분석에는 X 또는 Y 염색체에 상보적으로 결합하는 probe가 사용되었다. CISH 분석은 기존의 방법들보다 빠르고 쉬우며 비용이 적게 든다는 장점이 있다. 또한, CISH는 보다 정화한 정자의 선별을 가능하게 하는 것으로 나타났다. 본 연구에 따르면 CISH가 기존의 선별 방법들을 평가하는 기술로서만이 아니라 특정한 성별을 가진 포유동물의 생산에도 사용될 수 있을 것이다.

Growth Analysis of Chlamydomonas reinhardtii in Photoautotrophic Culture with Microdroplet Photobioreactor System (미세액적 광생물반응기를 활용한 광독립영양배양에서 Chlamydomonas reinhardtii의 성장성 분석)

  • Sung, Young Joon;Kwak, Ho Seok;Choi, Hong Il;Kim, Jaoon Young Hwan;Sim, Sang Jun
    • Korean Chemical Engineering Research
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    • v.55 no.1
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    • pp.80-85
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    • 2017
  • Recently, microalgae which can produce high-value products have attracted increasing attention for biological conversion of $CO_2$. However, low photosynthetic efficiency and productivity have limited the practical use of microalgae. Thus, we developed microdroplet photobioreactor for the analysis of photoautotrophic growth of model alga, Chlamydomonas reinhardtii. $CO_2$ transfer rate was increased by integrating micropillar arrays and adjusting height of microchamber. These results were identified by change of cell growth rate and fluorescence intensity. Lastly, the photoautotrophic growth kinetics of C. reinhardtii in microdroplet photobioreactor were investigated under different $CO_2$ concentrations and light intensities for 96 hours. As a result, microdroplet photobioreactor was efficient platform for isolation and rapid evaluation of microalgal strains which have enhanced productivity of high-value products and growth performance.